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1 ed active site residues essential for ligase adenylylation.
2 onounced lag phase in the progress of target adenylylation.
3 trogen-excess conditions due to excessive GS adenylylation.
4 397 to Phe, Ala, or Ser was found to prevent adenylylation.
5 induce changes previously thought to require adenylylation.
6 he hydroxyl group to AMP in a process termed adenylylation.
7 y is inversely proportional to the extent of adenylylation.
8 eleased after formation, and then rebind for adenylylation.
9 racteristics similar to the form obtained by adenylylation.
10 d associated with the phosphocholination and adenylylation activities of the enzymes AnkX and DrrA/Si
13 volves three distinct chemical steps: enzyme adenylylation, adenylyl transfer to DNA, and nick sealin
14 of a post-translational modification termed adenylylation/AMPylation in regulating signal transducti
15 ates the activity of glutamine synthetase by adenylylation and deadenylylation in response to signals
16 ranferase (ATase, EC 2.7.7.49) catalyzes the adenylylation and deadenylylation of glutamine synthetas
18 glutamine synthetase (GS) by catalyzing the adenylylation and deadenylylation of GS in response to s
19 Escherichia coli is regulated by the cyclic adenylylation and deadenylylation of Tyr-397 in each of
20 lysis revealed distinct requirements for the adenylylation and end-sealing reactions catalyzed by Lig
23 of mutational effects on the isolated ligase adenylylation and phosphodiester formation reactions rev
24 -285 and Phe-286 in the catalysis of the DNA adenylylation and phosphodiester synthesis reactions.
26 Examples of modification by N-acetylation, adenylylation and proteolytic processing were characteri
27 ling-defective mutants were active in ligase adenylylation and sealing a preadenylylated nick, thereb
29 example of a PII protein that is modified by adenylylation, and demonstrates that this reaction is pe
31 the deadenylylation of GS was to inhibit GS adenylylation, and this was due to the allosteric regula
32 f the nick 3' nucleoside for catalysis of 5' adenylylation; and (ii) EcoLigA's potential to embed mut
33 characterized the kinetics of DrrA-catalyzed adenylylation as well as SidD-catalyzed deadenylylation
39 ponent steps of the ligation pathway (ligase adenylylation, DNA adenylylation, and phosphodiester syn
40 in light of available structural data on the adenylylation domains of ATP- and NAD-dependent ligases.
43 domains of IbpA catalyze a unique reversible adenylylation event that uses ATP to add an adenosine mo
45 coli glutamine synthetase (GS) by reversible adenylylation has provided one of the classical paradigm
48 of proteins that undergo phosphorylation or adenylylation in signal transduction cascades might be s
49 and suggests the intriguing possibility that adenylylation in the pathogenic versus non-pathogenic my
51 scherichia coli and is subject to reversible adenylylation (inactivation) by a bifunctional GS adenyl
53 suppressed overall nick ligation and ligase adenylylation, it did not compromise sealing at a preade
54 tacts at the nick and with ATP during ligase adenylylation; (iv) the role of Phe-44 in forming the pr
55 s (k(step3) = 25 s(-1)) exceeds that for DNA adenylylation (k(step2) = 2.4 s(-1)) and that Mg(2+) bin
57 report that Escherichia coli RtcA catalyzes adenylylation of 5'-phosphate ends of DNA or RNA strands
63 autoinhibition and thus allowing subsequent adenylylation of its target, the DNA gyrase subunit GyrB
66 g these is DrrA/SidM, which catalyzes stable adenylylation of Rab1b, a regulator of endoplasmatic ret
67 sence of [alpha-(32)P]ATP, which resulted in adenylylation of Rho GTPases and cytoskeletal disruption
71 rates the second step of the ligase pathway (adenylylation of the 5'-PO4 strand) by a factor of 1000,
73 protein, and the reversible modification as adenylylation of the conserved tyro-sine 51 residue that
75 D) cofactor biosynthesis, which catalyze the adenylylation of the nicotinic acid mononucleotide (NaMN
76 mechanism based on the loop motions in which adenylylation of the Tyr397 loop reverses the effect of
77 Tase is Tyr-406, as indicated by the lack of adenylylation of the Y406F mutant, and, as expected, is
78 of Tyr-326 together with either nitration or adenylylation of Tyr-397 leads to inactivation of the en
81 by cyclic AMP (FIC)-domain enzymes catalyze adenylylation or other posttranslational modifications o
82 rovide important structural insight into the adenylylation reaction mechanism catalyzed by Fic domain
83 amide mononucleotide (NMN) occurs before the adenylylation reaction, which converts this alternative
86 sting that the 3' de-guanylylation and 5' de-adenylylation reactions follow the same pathway of nucle
87 bolished by mutation of the predicted lysine adenylylation site (Lys-165) in the C-terminal domain an
88 lutarate concentration, the regulation of GS adenylylation state by glutamine was sharper and occurre
91 s reconstituted bicyclic cascade system, the adenylylation state of GS was regulated reciprocally by
97 fs I, IV, and V and suffices for both enzyme adenylylation (step 1 of the ligation pathway) and phosp
98 tional effects on the isolated steps of Rnl1 adenylylation (step 1) and phosphodiester bond formation
99 3'OH/5'PO(4) nicks in duplex RNAs via ligase adenylylation (step 1), AMP transfer to the nick 5'PO(4)
100 eps of the ligation pathway including ligase-adenylylation (step 1), RNA adenylylation (step 2) and p
101 including ligase-adenylylation (step 1), RNA adenylylation (step 2) and phosphodiester bond synthesis
102 3'-OH nucleoside in the catalysis of DNA 5'-adenylylation (step 2) and phosphodiester synthesis (ste
103 dysfunctional by virtue of defects in ligase adenylylation: T163A, H167A, G168A, K186A, E230A, F281A
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