ライフサイエンスコーパス: adherent cell

1 tion and the projected area of an individual adherent cell.

2 ack the masses of single suspended cells and adherent cells.

3 t the growth and behavior of delicate/scarce adherent cells.

4 says of small numbers of either suspended or adherent cells.

5 ion of high-affinity LFA-1 in focal zones of adherent cells.

6 nding site, and target to focal adhesions in adherent cells.

7 chemoattractant formyl-Met-Leu-Phe (fMLP) in adherent cells.

8 ar networks to stress fibers are observed in adherent cells.

9 we explored integrin regulation of Bit-1 in adherent cells.

10 lly more scalable platform system than using adherent cells.

11 to the control of protrusion and motility in adherent cells.

12 properties, mass, and growth rate of single adherent cells.

13 riched for stem-like cells) when compared to adherent cells.

14 t the metaphase mitotic spindle of mammalian adherent cells.

15 g membrane (ILM), providing polarity cues to adherent cells.

16 ry (DHC) permits label-free visualization of adherent cells.

17 ng technique for transient pore formation in adherent cells.

18 at cell borders and the formation of tightly adherent cells.

19 ring in the Dictyostelium cleavage furrow of adherent cells.

20 anical models and physiological behaviors of adherent cells.

21 cell spreading are fundamental processes of adherent cells.

22 bute when the pallet arrays are used to sort adherent cells.

23 ures from primary NSCLC through isolation of adherent cells.

24 fabrication of arrays of pallets for sorting adherent cells.

25 oth suspended immobilized cells and cultured adherent cells.

26 ly low throughput for the analysis of single adherent cells.

27 known to affect cytoskeletal organization in adherent cells.

28 cell, as a function of varying the number of adherent cells.

29 kinase that localizes to focal adhesions in adherent cells.

30 hat recognizes fibronectin assembly sites on adherent cells.

31 e time prevent nonspecific interactions with adherent cells.

32 inase blocked Rac GTP loading in fibronectin-adherent cells.

33 he LR5 cells compared with drug-sensitive or adherent cells.

34 ells treated in suspension, as compared with adherent cells.

35 ic protein Bim were significantly reduced in adherent cells.

36 microrheometry (MBM) measurements on living adherent cells.

37 the activity of Fyn, but not Lyn, in laminin-adherent cells.

38 ia enhanced tyrosine phosphatase activity in adherent cells.

39 with MEK1 but not with MEK2 in serum-starved adherent cells.

40 bers and focal adhesions in fibronectin (FN)-adherent cells.

41 hat formed naturally at the ruffled edges of adherent cells.

42 (397)) when adherent to CBD compared with FN-adherent cells.

43 of these molecules restore Rac signaling in adherent cells.

44 tion from rapid to slow, similar to those of adherent cells.

45 ducing signal complex after CD95 ligation in adherent cells.

46 ctively than into macrophages or transfected adherent cells.

47 the treatments reduced the number of firmly adherent cells.

48 itiated in rounded interphase cells, but not adherent cells.

49 activation were still impaired compared with adherent cells.

50 uclear cells, gave rise to clusters of small adherent cells.

51 ive retrovirus particles onto the surface of adherent cells.

52 ent of thymocytes or splenocytes depleted of adherent cells.

53 c the stiffness and topographical profile of adherent cells.

54 plexes were lower in FN-adherent than in PLL-adherent cells.

55 eleton impact basal membrane fluctuations in adherent cells.

56 e used to perform mechanical measurements on adherent cells.

57 n on the metabolic activity and viability of adherent cells.

58 a remains after Arp2/3 complex inhibition in adherent cells.

59 sequently resulted in an increased number of adherent cells.

60 y requirements differ between suspension and adherent cells.

61 contractility found at the interface between adherent cells.

62 d 141-fold more in CSC spheres than daughter adherent cells.

63 s localized near the plasma membrane of live adherent cells.

64 ted osteosarcoma stem cells and the daughter adherent cells.

65 although they were not similar when grown as adherent cells.

66 phase in adherent monolayers or even single adherent cells.

67 ells/min, 2.07 +/- 0.04 versus 0.62 +/- 0.05 adherent cells/100 microm per minute; p < 0.001).

68 T cells, we examined WC1 endocytosis in the adherent cell 293T and Jurkat T cell lines using a fusio

69 selective isolation of single suspended and adherent cells according to the fluorescence imaging and

70 rk is used to model the deformability of the adherent cell actin cytoskeleton.

71 In contrast to the results with adherent cells, although MSP also prevented apoptosis of

72 ositioned perpendicular to the surface of an adherent cell and a constant-rate aspiration pressure is

73 important regulator of integrin signaling in adherent cells and accordingly its activity is significa

74 th delays and defective spindle formation in adherent cells and cell death in suspension.

75 ween paxillin and ILK occurs in vivo in both adherent cells and cells in suspension.

76 are underlain by different configurations of adherent cells and extracellular matrix (ECM).

77 utrophils with confocal microscopy of single adherent cells and flow cytometry of cell suspensions th

78 paxillin localizes to the focal adhesions of adherent cells and has been implicated in the regulation

79 e the low throughput of CE-based analysis of adherent cells and increase its utility in evaluating ce

80 , 0.1 and 1.0 microM was observed at 24 h in adherent cells and increased at 48 h.

81 ogy is appropriate for label-free imaging of adherent cells and is particularly suitable for reportin

82 the cyclin A promoter was seen in both ts13 adherent cells and Jurkat T lymphocytes.

83 -EPC solution density affected the number of adherent cells and larger cells preferentially adhered a

84 erized pulses and waves of Rho activation in adherent cells and proposed excitable Rho signaling netw

85 ngements of beta1-integrin subunits occur in adherent cells and that conversion from a bent to extend

86 activation in cells in suspension but not in adherent cells and the CRBP suppression of anchorage-ind

87 ith shear stress, with the maximum number of adherent cells and the shear stress at maximum adhesion

88 The mechanical interaction between adherent cells and their substrate relies on the formati

89 essed as a dephosphorylated protein in round adherent cells and was phosphorylated at the onset of ch

90 optimized it for fibroblasts (as a model of adherent cell) and rat basophilic leukemia cells (as a m

91 flow, vasoconstriction, vascular plugging by adherent cells, and hemorrhages.

92 This 1-2-d protocol is applicable to adherent cells, and it is adaptable for use with several

93 high resting Ca(2+) levels, rounded, poorly adherent cells, and loss of membrane integrity.

94 activity of Lyn, but not Fyn, in vitronectin-adherent cells, and the activity of Fyn, but not Lyn, in

95 umber of rolling cells, the number of firmly adherent cells, and the duration of both rolling and fir

96 cid transporter protein xCT for infection of adherent cells, and the xCT molecule is part of the cell

97 ned suspensions of cells or small numbers of adherent cells ( approximately 10).

98 ce when force-indentation curves obtained on adherent cells are analysed using our combined strategy,

99 density-dependent pathway control: when the adherent cells are at low density, the culture medium is

100 d mechanical interactions between matrix and adherent cells are important for cellular remodeling as

101 ndependent growth, and their growth rates as adherent cells are increased.

102 In this paper, the stiffness of single adherent cells are optomechanically characterized using

103 the RBR binding partner is internalized when adherent cells are placed in suspension.

104 lands acting as drug depots colocalized with adherent cells are surrounded by a nonfouling background

105 nduced stiffening over tens of minutes after adherent cells are suspended.

106 Adherent cells are synaptophysin-negative and express pR

107 Firmly adherent cells arresting for at least 0.2 s were well fi

108 A physical model of the adherent cell as a contractile gel under a uniform bound

109 ys of micropallets have been used to pattern adherent cells as well to sort mixtures of cells.

110 acterize the mechanical properties of single adherent cells at multiple frequencies with high through

111 Local signaling by implant-adherent cells at the implant-bone interface may indirec

112 tool, which enables the selection of certain adherent cells based on their fluorescence, and their sp

113 ely 1 nM when assayed as monolayers of 10(6) adherent cells bathed in 1 ml of medium.

114 ided novel methods for biological studies of adherent cells because the small features of these new b

115 ls, cells arrested in mitosis, and naturally adherent cells brought into suspension, stiffen and beco

116 cell migration enabling rear de-adhesion of adherent cells by cleaving structural components of the

117 Cell binding was confirmed by visualizing adherent cells by fluorescence microscopy.

118 Thus Met promotes survival of laminin-adherent cells by maintaining integrin alpha3beta1 via a

119 Here we show that immobilizing non-adherent cells by optical tweezers is sufficient to achi

120 an important role in mechanotransduction in adherent cells by providing a means to sense the directi

121 ) cascade functions as a survival pathway in adherent cells by regulating c-Flip expression.

122 Imaging non-adherent cells by super-resolution far-field fluorescenc

123 select and 'paint' individual Pdot-labelled adherent cells by turning on their fluorescence, then pr

124 n seen in sickle cell disease as mediated by adherent cells can now be rationalized, and surprisingly

125 orted persistent rotational behavior between adherent cell-cell pairs cultured on micropatterned subs

126 tools for optimizing DHC for kinetic single adherent cell classification.

127 nd led to the formation of immobile, tightly adherent cell colonies.

128 compartment as they are isolated within the adherent cell component.

129 Eight hours following removal from adherent cell contact BLIN cells undergo a decrease in m

130 As few as five adherent cells could be specifically selected for analys

131 in filament assembly in the lamellipodium in adherent cells crawling on planar 2-dimensional (2D) sub

132 es are fundamentally reliant on high-quality adherent cell culture, but current methods to cryopreser

133 -cooling cryomicroscopy, IIF was measured in adherent cells cultured in micropatterned linear constru

134 liably measuring kinase activities of single adherent cells cultured in nanoliter volume microwells.

135 second pulsed laser microbeam irradiation in adherent cell cultures.

136 At the leading edge of adherent cells, curvature waves are associated with prot

137 e show that knockdown of endogenous Bit-1 in adherent cells decreased cell survival and re-expression

138 elevates RhoA-GTP levels in nonadherent and adherent cells, delays maximal RhoA activation upon cell

139 Human dental pulp cells (DPCs), adherent cells derived from dental pulp tissues, are pot

140 iation of lung fibroblasts is blocked in non-adherent cells despite the preservation of TGF-beta rece

141 ass spectrometry (MS) can be used to analyze adherent cells directly, while still attached to the cul

142 Because adherent cells do not have a simple and consistent shape

143 rom resting, nonadherent cells to activated, adherent cells during these cell-mediated responses.

144 and rear to enable productive locomotion of adherent cells during wound repair and tumor invasion.

145 hey derive from the original tumors; 0.5% of adherent cells express detectable levels of CD133.

146 ration, collection, and expansion of single, adherent cells from a heterogeneous cell population.

147 cells that can be isolated as proliferating, adherent cells from bones.

148 Mononucleated and adherent cells from donor-matched rat dental pulp (denta

149 e process of isolating clonal populations of adherent cells from heterogeneous mixtures retaining the

150 Adherent cells from spleens of infected BR mice produced

151 esent a microfluidic probe that lyses single adherent cells from standard tissue culture and captures

152 ubtype of apoptosis induced by detachment of adherent cells from the extracellular matrix.

153 IL-12 production by adherent cells from the spleens of burn or sham burn mic

154 sis, whereas wild-type survivin protects non-adherent cells from TNF-alpha-induced apoptosis.

155 Adherent cells generate forces through acto-myosin contr

156 The adherent cells generated contractile forces that deform

157 ight-sheet fluorescence microscopy (LSFM) of adherent cells, glass substrates are advantageously rota

158 rian cancer cells decreased adherent and non-adherent cell growth and induced phenotypic changes incl

159 We found that: (i) CRBP did not inhibit adherent cell growth but suppressed foci formation in po

160 Single-cell transfection of adherent cells has been accomplished using single-cell e

161 bstrate, is reverse-transfected locally into adherent cells, has become a standard tool for parallel

162 for KSHV are widely distributed on cultured adherent cells, (ii) that latency is the default pathway

163 easures the total mass of single or multiple adherent cells in culture conditions over days with mill

164 it has not been possible to track individual adherent cells in physiological conditions at the mass a

165 the reorganization of actin stress fibers in adherent cells in response to diverse patterns of mechan

166 than fluorescence or cell sorting to analyze adherent cells in situ.

167 stable maintenance of a small population of adherent cells in spite of HMW adhesin specific antibody

168 measure the cell dry mass of many individual adherent cells in various conditions, over spatial scale

169 r encapsulating, manipulating, and observing adherent cells in vitro.

170 eas integrin antagonists induce apoptosis of adherent cells in vivo.

171 uncharacterised mixed population of plastic-adherent cells) in the treatment of neural injury.

172 %, 42%, and 44%, respectively, compared with adherent cells, in vitro.

173 n glass slides, overlaid with a monolayer of adherent cells, incubated to allow reverse transfection,

174 Ectopic expression of KSHV TK in adherent cells induced striking morphological changes an

175 Restoration of c-Myc in non-adherent cells induces the expression of E2F-1, and hype

176 Furthermore, when cocultured with adherent cells infected with the LA vector, the human T-

177 These biofilm colonies release adherent cells into the medium, and the released cells c

178 The proliferation of all nontransformed adherent cells is dependent upon the development of mech

179 d elasticity on collective cell migration by adherent cells is highly physiologically relevant, but r

180 Although mitotic rounding of adherent cells is necessary for proper cell division, ou

181 chanical cues affect collective migration of adherent cells is not well understood.

182 (n = 6; 50 to 60 kg), selected culture-flask adherent cells, labeled them with the gene for green flu

183 Adherent cells lacking alpha6beta4 require an inducer, s

184 stems which enable impedance measurements on adherent cell layers under label-free conditions are con

185 d virus replication in a panel of T-cell and adherent cell lines and in primary lymphocytes and monoc

186 and SFV-pseudotyped lentivirus vectors into adherent cell lines can be significantly increased by us

187 ort that the binding of MYXV or VACV to some adherent cell lines could be partially inhibited by hepa

188 Studies using adherent cell lines have shown that glucose transporter-

189 S-transferase (GST) isozyme, hGSTA4-4, into adherent cell lines HLE B-3 and CCL-75, by either cDNA t

190 transduction of reporter genes is reduced in adherent cell lines that are deficient in cellular DNA-r

191 KSHV can establish latent infection in many adherent cell lines, including human and nonhuman cells

192 hat it enhances the DMSO cryopreservation of adherent cell lines.

193 trate that FADD is mainly nuclear in several adherent cell lines.

194 or technology is deemed only compatible with adherent cell lines.

195 method for normalizing metabolomic data from adherent cell lines.

196 special mode of apoptosis induction in which adherent cells lose contact with the extracellular matri

197 for the first time, was readily apparent in adherent cells, lost following cellular detachment, and

198 In the vitronectin-adherent cells, Lyn was associated preferentially with a

199 3) An adherent cell may influence the flow streams in its vici

200 7 adherent cells/min, with NMA vs. 4.3+/-0.7 adherent cells/min, after L-arginine; n = 5; p < .05).

201 leukocyte adhesion in septic rats (5.4+/-0.7 adherent cells/min, with NMA vs. 4.3+/-0.7 adherent cell

202 this phenotype could proliferate and produce adherent cell monolayers capable of chondrogenic, osteog

203 ed cell adhesion to extracellular matrix and adherent cell monolayers plays a key role in many physio

204 alpha-tubulin modifications are critical for adherent cell motility and implicated in numerous pathol

205 However, in non-adherent cells no molecular mechanism has yet been propo

206 hin mesenchymal stem cells-as a prototypical adherent cell-nonmuscle myosin IIA and vimentin are just

207 proviral genomes from single-round-infected adherent cells of lymphocytes by using a lambda phage li

208 ogy to measure the growth rate of individual adherent cells of various sizes has been lacking.

209 ve measurement of the dynamic fluctuation of adherent cells, often referred to as the cellular microm

210 which determines traction forces exerted by adherent cells on a thin, elastic polyacrylamide gel emb

211 n on a density gradient and then cultured as adherent cells on plastic that proliferated in culture t

212 hours showed a significant reduction of the adherent cells on the microslides.

213 Traction forces exerted by adherent cells on their microenvironment can mediate man

214 In adherent cells only, mDia1 and VASP also contribute to f

215 ized either by decreasing the density of the adherent cells or by bringing the cells into suspension,

216 ediated apoptosis was abolished by depleting adherent cells or Mac-1(+) spleen cells or by inhibiting

217 Furthermore, incubation of marrow adherent cells or mesenchymal stem cells with LCN2 incre

218 ed alveolar macrophages and peripheral blood adherent cells (PBAC).

219 ours after intracardiac injection, and a few adherent cells persisted until colonization occurred.

220 eflected by decreased gene expression in the adherent cell population.

221 Examination of plastic-adherent cell populations in bronchoalveolar lavage samp

222 or the nontargeted in situ analysis of small adherent cell populations.

223 Unlike primary adherent cells, primary and secondary tumors heterogeneo

224 IL-1beta decreases the adherent cells, produces DNA ladders, but fails to cleav

225 F stimulation (83 pm, 10 min) of vitronectin-adherent cells promoted the specific recruitment of inte

226 so demonstrate that the on-chip assay, using adherent cells, provides the possibility of faster scree

227 Adherent cells remodel their cytoskeleton, including its

228 take by 4.4 and 9.9 fold in non-adherent and adherent cells, respectively.

229 o visualize the freezing process in pairs of adherent cells, revealing that the initial IIF event in

230 re captured from flow, a small proportion of adherent cells rolled, and the majority transmigrated th

231 The computational model is assessed using an adherent cell, rolling and deforming along the vessel wa

232 Ectopic expression of FOXD3 in non-adherent cells significantly reduced cell death in respo

233 However, this effect of adaphostin in adherent cells/solid tumor models has not been examined.

234 including cells embedded inside a 3D matrix, adherent cells subjected to shear flows, and cells insid

235 In migrating adherent cells such as fibroblasts and endothelial cells

236 We and others previously reprogrammed human adherent cells, such as postnatal fibroblasts to iPS cel

237 concentrated in clusters in the rear of the adherent cells, suggesting that [Ca(++)](i) transients a

238 Epithelia are polarized layers of adherent cells that are the building blocks for organ an

239 eet-like, leading edge protrusions in firmly adherent cells that contain Arp2/3-generated dendritic a

240 ax5(-/-) spleen cells yields a population of adherent cells that grow spontaneously in culture withou

241 growth factor-beta were localized to implant-adherent cells that included macrophages and foreign bod

242 surfaces of cells that are normally tightly adherent, cells that ingress during gastrulation failed

243 all-less plate produced similar results with adherent cells, the advantage of the DropArray technolog

244 In isolated adherent cells, the orientation of mitotic spindles is s

245 uations are affected by cellular activity in adherent cells, their spatial regulation and the corresp

246 determined leader-trailer polarized pair of adherent cells, they are capable of migrating individual

247 Since cell shape is maintained in weakly adherent cells, this state might induce survival signals

248 ates the formation of actin stress fibers in adherent cells through activation of its effector protei

249 This transformation of adherent cells to a motile phenotype has been associated

250 The pallet array system permits adherent cells to be inspected using conventional micros

251 ries, or micropipets positioned near single, adherent cells to increase transiently the membrane perm

252 The ability of adherent cells to later detach and again become prolifer

253 assays are based on the inherent ability of adherent cells to move into adjacent cell-free areas, th

254 onal motion seen in systems ranging from two adherent cells to multicellular assemblies.

255 erms of their cytotoxicity on suspension and adherent cells to prove their applicability as cancer na

256 beta1A integrin is necessary to protect non-adherent cells transduced with survivin from TNF-alpha-i

257 revent apoptosis induced by TNF-alpha in non-adherent cells transduced with survivin.

258 ize ERK to the nucleus in suspended cells or adherent cells treated with CCD.

259 otein apparatus, applicable as well to other adherent cell types and amyloid compounds.

260 an cell lines demonstrated that a variety of adherent cell types could be cultured and effectively sp

261 ion methods and overcomes the need for flat, adherent cell types that are required for immunofluoresc

262 Ebola virus infects a wide variety of adherent cell types, while nonadherent cells are found t

263 These studies demonstrate that biomaterial-adherent cells undergo material-dependent apoptosis in v

264 Conversely, the proportion of adherent cells undergoing apoptosis was increased signif

265 Adherent cells use forces at the cell-substrate interfac

266 we developed a method to displace nuclei in adherent cells using centrifugal force.

267 s of localized O(2) gradients in cultures of adherent cells, using three phosphorescent Pt-porphyrin

268 s with an emphasis on in vitro delivery into adherent cells utilizing mechanical penetration or elect

269 The number of adherent cells varied with shear stress, with the maximu

270 the stiffness optima for different kinds of adherent cells vary widely, it is generally true that ce

271 e adenylate energy charge (0.90 +/- 0.09 for adherent cells vs 0.09 +/- 0.03 for suspended cells).

272 The effect of shear forces on the adherent cells was evaluated in a flow chamber.

273 Second, whereas HIV-1 fusion with adherent cells was insensitive to actin inhibitors, post

274 s load of RV-B (RV-B52, RV-B72, or RV-B6) in adherent cells was lower than that of RV-A or RV-C.

275 s approach, the metabolic activity of single adherent cells was monitored continuously over time, and

276 electin was unaltered although the number of adherent cells was reduced by 60%.

277 en chromatin landscapes with a low number of adherent cells, we demonstrate that the BAF complex is e

278 To test the applicability of this model in adherent cells, we used a fluorescent resonance energy t

279 With this aim, adherent cells were analyzed in the SECM feedback mode i

280 choalveolar lavage was performed and plastic-adherent cells were cultured, characterized and then del

281 Rapidly growing cell suspension and adherent cells were effectively labeled by means of endo

282 t or nonadherent cultures, and in both cases adherent cells were found to be significantly more susce

283 Adherent cells were grown in either 96- or 384-well plat

284 locity was markedly decreased and numbers of adherent cells were increased in the mutant mice.

285 te rolling velocity (Vwbc) and the number of adherent cells were not altered with normal ventilation

286 ripheral blood mononuclear cells (PBMCs) and adherent cells were obtained from 27 patients with major

287 Adherent cells were quantified using crystal violet.

288 ty of the first group was observable only in adherent cells when confluence was approximately 100%.

289 Furthermore, they were more tumorigenic than adherent cells when grafted to mice.

290 s exhibit extensive MCSP-rich microspikes on adherent cells, where it also colocalizes with alpha4 in

291 ized enzymes and reversible encapsulation of adherent cells--which offer promise for incorporation wi

292 fore, present the same topographical cues to adherent cells while varying substrate rigidity only thr

293 Treatment of ECM-adherent cells with EGF, or overexpression of EGFR or My

294 probes (TPs) that report traction forces of adherent cells with high spatial resolution, can in prin

295 r could do so less efficiently in lysates of adherent cells with increased ERK activity.

296 cell interactions via direct manipulation of adherent cells with micrometer-scale precision.

297 er liquid, and/or inability to select single adherent cells with specific phenotypes.

298 to screen and then isolate adherent and non-adherent cells with very high efficiency and excellent v

299 es that recognize markers expressed on live, adherent cells within a microfluidic channel.

300 optosis and differentiation of the remaining adherent cells without affecting their cell cycle progre