ライフサイエンスコーパス: adrenodoxin

1 lian counterparts, adrenodoxin reductase and adrenodoxin.

2 residues also crucial in P450scc for binding adrenodoxin.

3 rate ferredoxin family which includes bovine adrenodoxin.

4 ochrome P450 oxidoreductase or mitochondrial adrenodoxin.

5 The purified P450MT2 showed a preference for adrenodoxin + adrenodoxin reductase electron donor syste

6 associated fusion protein is functional with adrenodoxin + adrenodoxin reductase.

7 . the genes and proteins for P450scc enzyme, adrenodoxin, adrenodoxin reductase and MLN64.

8 activity of recombinant h-P450cc24 required adrenodoxin, adrenodoxin reductase, and NADPH.

9 It requires electron transfer from the adrenodoxin/adrenodoxin reductase system to catalyze the

10 dentical and very similar to those of bovine adrenodoxin (Adx) and Escherichia coli ferredoxin.

11 ystal structure of the homologous ferredoxin adrenodoxin (Adx) and loose restraints determined from p

12 e vertebrate-type ferredoxins, the mammalian adrenodoxin (Adx) and the bacterial ferredoxins putidare

13 Adrenodoxin (Adx) belongs to the family of Cys(4)Fe(2)S(

14 Co-expression with wild type P4501A1 and adrenodoxin (Adx) cDNAs resulted in 5-7-fold higher eryt

15 (cAMP) regulates transcription of the bovine adrenodoxin (Adx) gene in the adrenal cortex.

16 and spectral studies show that the wild-type adrenodoxin (Adx), but not the mutant Adx, binds to P450

17 o mitochondria (P450MT2) which interact with adrenodoxin (Adx), cytochrome P450 reductase (CPR) and b

18 oxycholesterol and the protein redox partner adrenodoxin (Adx).

19 using hydrophobic chromatography followed by adrenodoxin affinity binding.

20 responsible for its observed preference for adrenodoxin and adrenodoxin reductase electron transfer

21 g recombinant human CYP11B2 as well as human adrenodoxin and adrenodoxin reductase was established to

22 In concert with its cofactors, adrenodoxin and adrenodoxin reductase, and the transcrip

23 450 2D6 (CYP2D6), supported by mitochondrial adrenodoxin and adrenodoxin reductase, can efficiently c

24 hondrial enzyme was supported exclusively by adrenodoxin and adrenodoxin reductase.

25 icosatetraenoic acid when reconstituted with adrenodoxin and adrenodoxin reductase.

26 YP1B1 supported by mitochondrial ferredoxin (adrenodoxin) and ferredoxin reductase showed high aryl h

27 embrane-bound enzymes: aldosterone synthase, adrenodoxin, and adrenodoxin reductase.

28 tructure contains a large dipole moment like adrenodoxin, and appears to have a similar interaction d

29 reconstituted with the iron-sulfer protein, adrenodoxin, and the flavoprotein, adrenodoxin reductase

30 y, to serine also decreased the affinity for adrenodoxin approximately 20-fold.

31 results in virtually complete inhibition of adrenodoxin binding, in P450c27 there are three of such

32 s of the approximately 30-fold difference in adrenodoxin binding, two sets of P450c27 mutants were ge

33 354A/K358A/R418S mutation profoundly reduced adrenodoxin binding.

34 ved in mitochondrial P450s are important for adrenodoxin binding.

35 utations of one set were within the putative adrenodoxin-binding site containing conserved lysine res

36 ximately 20-fold increase in apparent Ks for adrenodoxin, confirming that these two positively charge

37 posed of 105 residues with similarity to the adrenodoxin family of [2Fe-2S] bacterial ferredoxins.

38 ture of a closely related ferredoxin, bovine adrenodoxin, has been published.

39 Lys-354, Lys-358, and Arg-418) important for adrenodoxin interaction.

40 n ferredoxin, the human equivalent of bovine adrenodoxin, is a small iron-sulfur protein with one [2F

41 multiple ancient connections between Fe2S2- (adrenodoxin-like) and heme- (cytochrome c) binding domai

42 fer is a shared mechanistic feature with the adrenodoxin P450 and photosynthetic electron-transfer sy

43 minal domains to [2Fe-2S] ferredoxins of the adrenodoxin/putidaredoxin subfamily of ferredoxins.

44 ximal surface identifies the determinants of adrenodoxin recognition as a constellation of conserved

45 e intensively studied monoflavin reductases, adrenodoxin reductase (AdR) and ferredoxin-NADP+ reducta

46 proteins, cytochrome P450 11A1 (CYP11A1) and adrenodoxin reductase (AdR).

47 ribe the Drosophila dare gene, which encodes adrenodoxin reductase (AR).

48 dox couple and their mammalian counterparts, adrenodoxin reductase and adrenodoxin.

49 nd proteins for P450scc enzyme, adrenodoxin, adrenodoxin reductase and MLN64.

50 450MT2 showed a preference for adrenodoxin + adrenodoxin reductase electron donor system and exhibite

51 its observed preference for adrenodoxin and adrenodoxin reductase electron transfer proteins.

52 Finally, the expression of Drosophila Adrenodoxin Reductase increases during mid-oogenesis and

53 man CYP11B2 as well as human adrenodoxin and adrenodoxin reductase was established to help maximize t

54 recombinant h-P450cc24 required adrenodoxin, adrenodoxin reductase, and NADPH.

55 protein, adrenodoxin, and the flavoprotein, adrenodoxin reductase, and show the NADPH and time-depen

56 concert with its cofactors, adrenodoxin and adrenodoxin reductase, and the transcription factor ster

57 , supported by mitochondrial adrenodoxin and adrenodoxin reductase, can efficiently catalyze the meta

58 Presence of P450scc, adrenodoxin reductase, cytochrome P450 17-hydroxylase (P

59 ymes: aldosterone synthase, adrenodoxin, and adrenodoxin reductase.

60 was supported exclusively by adrenodoxin and adrenodoxin reductase.

61 ion protein is functional with adrenodoxin + adrenodoxin reductase.

62 acid when reconstituted with adrenodoxin and adrenodoxin reductase.

63 Adrenodoxin reduction and hydrogen abstraction both cont

64 higher affinity for the common redox partner adrenodoxin than another mitochondrial P450, P450scc (pr

65 oteins, CsmI, CsmJ, and CsmX, are related to adrenodoxin-type ferredoxins that ligate [2Fe-2S] cluste

66 Ferric P450 27C1 reduction by adrenodoxin was 3-fold faster in the presence of the sub

67 Oxidized human [2Fe-2S] ferredoxin (adrenodoxin) was used to test the approach.

68 n the oxidized (Adx(o)) and reduced (Adx(r)) adrenodoxin were probed by measurement of (15)N relaxati