ライフサイエンスコーパス: afadin

1 vated contractility by increasing junctional afadin.

2 AJ reannealing mediated by the Rap1 effector afadin.

3 ynapses through the scaffolding protein AF-6/afadin.

4 y associates with the nectin-binding protein afadin.

5 d with the cytoplasmic actin-binding protein afadin.

6 h protein harbors a cryptic binding site for afadin.

7 It was also previously shown that AF-6 (afadin), a PDZ domain-containing protein, associates wit

8 In this study, we find that afadin, a component of N-cadherin.beta-catenin.alpha-N-c

9 that lumen formation and elongation require afadin, a nectin adaptor protein implicated in adherens

10 Infectious-center growth is facilitated by afadin, a protein connecting the adherens junction and t

11 We propose that afadin acts as a robust protein scaffold that maintains

12 Together, these results suggest that afadin acts upstream of the Par complex to regulate the

13 neuroblasts), the scaffolding protein Canoe (Afadin/Af-6 in mammals) regulates spindle orientation, b

14 vo and phosphorylates Canoe, the ortholog of Afadin/AF-6, in vitro.

15 cell adherens junctions and interacts with l-afadin, an F-actin-binding protein.

16 through their cytoplasm-associated proteins, afadin and catenins, it is not fully understood how nect

17 we show that the adherens junction proteins afadin and CDH2 are critical for the control of cell pro

18 ll-cell adhesion sites after both the nectin-afadin and E-cadherin-catenin systems had been assembled

19 nin-binding protein that connects the nectin-afadin and E-cadherin-catenin systems through alpha-acti

20 hat LMO7 was associated with both the nectin-afadin and E-cadherin-catenin systems.

21 Both Afadin and PDZ-GEF2 colocalized and coimmunoprecipitated

22 tion facilitates formation of a complex with Afadin and PDZ-GEF2 that activates Rap1A, which regulate

23 ted with the PDZ domain-containing molecules Afadin and PDZ-guanine nucleotide exchange factor (GEF)

24 Our study uncovers essential roles of Afadin and RhoA in pancreatic central lumen morphogenesi

25 In this issue, Choi et al. show that afadin and ZO-1 regulate tension and maintain zonula adh

26 lial permeability via association with ZO-2, afadin, and PDZ-GEF1 to activate Rap2c and control contr

27 physical interactions between MAGI-1, AFD-1/afadin, and SAX-7/L1CAM, which are part of a functional

28 iates directly with ZO-2 and indirectly with afadin, and this complex, along with PDZ-GEF1, activates

29 These results indicate that LMO7 is an afadin- and alpha-actinin-binding protein that connects

30 of the human LIM domain only 7 (LMO7) as an afadin- and alpha-actinin-binding protein.

31 e Rap1 and the actin-junctional linker Canoe/afadin are essential for polarity establishment, as both

32 the synaptic localization of nectin-1 and l-afadin are F-actin-dependent and that the shedding of ne

33 Our findings identify nectins and afadin as components of the reelin signaling pathway and

34 data demonstrate that activation of the Rap1-afadin axis is a physiological mechanism driving restora

35 elix bundles, both of which are required for afadin binding.

36 also generated a nectin-4 mutant without the afadin-binding site in its cytoplasmic tail.

37 lpha-catenin, residues 385-651, contains the afadin-binding site.

38 upts the synaptically localized nectin-1 and afadin cluster at an early stage and elicits nectin-1 ec

39 Nectin-1 and afadin cluster at developing synapses between hippocampa

40 These nectin-afadin clusters uniformly colocalize with N-cadherin-cat

41 The nectin-afadin complex also localizes to AJs and links to the cy

42 elial cells, MV spread requires the nectin-4/afadin complex and is based on cytoplasm transfer betwee

43 its role in promoting cadherin recruitment, afadin deletion resulted in 70% fewer and less intense N

44 ether, these results support a model whereby afadin determines lumen placement by directing apical-ba

45 We further demonstrate that afadin directly interacts with AMPA receptors and that l

46 We show that loss of the scaffolding protein Afadin disrupts de novo lumenogenesis and lumen continui

47 age and roles of the linkage regulator Canoe/afadin during Drosophila germband extension (GBE), a con

48 we studied the distribution of nectin-1 and afadin during hippocampal synapse formation using cultur

49 between nectin-1 and the cytoplasmic protein afadin for HSV entry and spread as well as the effects o

50 ed to dissociation of cell junction scaffold afadin from the adherens junctions.

51 oncomitant shuttling of the scaffold protein afadin from the cytosol to the nucleus and synapses.

52 Mammalian afadin has been suggested to be essential for adhesion a

53 In contrast, Drosophila melanogaster's afadin homologue Canoe (Cno) has suggested roles in sign

54 Mice that lack afadin in nephron precursors show evidence of Par3-expre

55 Forced expression of recombinant afadin in pulmonary endothelium attenuated CS-induced VE

56 Absence of afadin in vivo leads to misorientation of apical-basal c

57 Thus, afadin is a key intracellular signaling molecule for cad

58 Afadin is a Rap-regulated, actin-binding protein that pr

59 Phosphorylation studies demonstrate that afadin is a substrate for AMPK.

60 Here, we demonstrate that afadin is required for lumen continuity by orienting the

61 ently found that the F-actin-binding protein afadin is required for lumen continuity in developing re

62 Furthermore, we demonstrate that afadin is required for Par complex formation.

63 Collectively, these data suggest that afadin is required for the maintenance of dendritic stru

64 noe (Cno), the Drosophila orthologue of AF-6/Afadin, is essential for Slit-Robo signaling.

65 Although ZO/afadin knockdown did not prevent contractile array assem

66 acts with the putative actin regulator AFD-1/afadin; knocking down magi-1 or afd-1 function in a hypo

67 Absence of afadin led to delayed and diminished integration of nect

68 that LMO7 localized at cell-cell AJs, where afadin localized, in epithelial cells of rat gallbladder

69 sing an in vitro 3D cyst model, we find that afadin localizes to the cell cortex adjacent to the spin

70 ether with the intracellular binding partner afadin, mediate adhesion and signaling at a variety of i

71 role in mediating synaptogenesis, we deleted afadin (mllt1), using a conditional allele, in postmitot

72 The alpha-catenin fragment 385-651 binds afadin more strongly than the full-length protein, sugge

73 Critically, blocking afadin nuclear accumulation attenuated activity-dependen

74 Moreover, activity-dependent afadin nuclear translocation coincides with phosphorylat

75 Activity-dependent afadin nuclear translocation peaked 2 h post-stimulation

76 Inactivation of afadin or CDH2 in the dorsal telencephalon leads to a ph

77 Loss of JAM-A, Afadin, or PDZ-GEF2, but not ZO-1 or PDZ-GEF1, similarly

78 equired for the colocalization of the nectin/afadin/ponsin adhesion system to adherens junctions, and

79 pletion of the actomyosin regulator RhoA and Afadin results in defects in the central lumens and arre

80 Furthermore, the nectin-afadin system is required for the deposition of tight ju

81 motes neuronal Cdh2 function via nectin3 and afadin, thus directing the broadly expressed homophilic

82 Furthermore, association of PDZ-GEF2 with Afadin was dependent on the expression of JAM-A.

83 To assess the relevance of the protein afadin, which connects nectin-4 to the actin cytoskeleto