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1 teins to aid in their purification via metal affinity chromatography.
2 cells and purified by immobilized metal ion affinity chromatography.
3 The enzyme was purified using cobalt ion affinity chromatography.
4 ed immunoprecipitation and immobilized metal affinity chromatography.
5 lated from crude cell membrane fractions via affinity chromatography.
6 he detergent Fos-choline-12, and purified by affinity chromatography.
7 coli and purified to homogeneity by means of affinity chromatography.
8 per motif transcription factor, by G-box DNA affinity chromatography.
9 ied by nickel-nitrilotriacetic acid (Ni-NTA) affinity chromatography.
10 f immobilized metal affinity and metal oxide affinity chromatography.
11 m a variety of species and tissue sources by affinity chromatography.
12 binant His(6)-tagged TcNr was purified by Ni affinity chromatography.
13 and easily purified from Escherichia coli by affinity chromatography.
14 glycated species can be enriched by boronate affinity chromatography.
15 tag, and the protein was purified by nickel affinity chromatography.
16 in surface labeling followed by streptavidin-affinity chromatography.
17 antibodies were removed from immune sera by affinity chromatography.
18 rotein could be purified in a single step by affinity chromatography.
19 and mTORC2 from HEK-293 cells using FLAG-M2 affinity chromatography.
20 Neurexin1 as a receptor for LRRTM2 based on affinity chromatography.
21 endothelial Ag, in this study purified by Ab-affinity chromatography.
22 nt PrtP copurifies with PrcB-6xHis in nickel affinity chromatography.
23 purified from a bacterial host using nickel affinity chromatography.
24 chromatography and immobilized bTf antibody affinity chromatography.
25 (DE3) and purified the enzyme by cobalt ion affinity chromatography.
26 s purified from human placental membranes by affinity chromatography.
27 rs and CUG-BP1 to the exonic silencer by RNA affinity chromatography.
28 g to CCPs 7-8 and 19-20 bind well in heparin-affinity chromatography.
29 ermus thermophilus HB27 was purified by Ni2+ affinity chromatography.
30 d Cryptosporidium hominis sporozoites by Gal-affinity chromatography.
31 d with wild-type (WT) p51 RT and purified by affinity chromatography.
32 with mild detergents and purified rapidly by affinity chromatography.
33 ich might have been co-purified during metal affinity chromatography.
34 sed recombinantly in E. coli and purified by affinity chromatography.
35 estimates of dissociation constants and FcRn affinity chromatography.
36 uent pulldown experiments with biotin-avidin affinity chromatography.
37 lene glycol precipitation followed by lectin affinity chromatography.
38 re exploited to purify the native protein by affinity chromatography.
39 red by surface plasmon resonance and heparin affinity chromatography.
40 and purified by nickel-nitrilotriacetic acid affinity chromatography.
41 lot and ELISA, and OPN was purified using Ni affinity chromatography.
42 nium sulfate precipitation, ion exchange and affinity chromatography.
43 e, A1AT (Zemaira) or M-type A1AT purified by affinity chromatography.
44 sed in a heterologous system and purified by affinity chromatography.
45 ividual conformers could be isolated by bNAb affinity chromatography.
46 xpressed in Escherichia coli and purified by affinity chromatography.
47 mplex purified under anaerobic conditions by affinity chromatography.
48 chia coli and then purified by metal chelate affinity chromatography.
49 erexpressed protein by immobilized metal ion affinity chromatography.
50 in E. coli, and purified with Ni-NTA agarose affinity chromatography.
51 otein (CabP) in S. pneumoniae using c-di-AMP affinity chromatography.
52 was purified by nickel-nitrilotriacetic acid affinity chromatography.
53 PSF) as an interacting protein with Fox-3 by affinity-chromatography.
54 que that couples isotachophoresis (ITP) with affinity chromatography (AC) to achieve rapid, selective
55 dy of coupling of isotachophoresis (ITP) and affinity chromatography (AC) to effect rapid, selective
58 cific IgGs from four representative sera via affinity chromatography, allowing us to determine the co
60 ication as well as tandem size exclusion and affinity chromatography analyses provided the first bioc
61 specifically interacts with DnaA protein by affinity chromatography and a solid phase binding assay,
62 nrich the modified peptides by avidin-biotin affinity chromatography and analyze them by nanoRP-UPLC-
63 labeled proteins are purified by mini-scale affinity chromatography and analyzed by gel electrophore
64 ng Fab/Rev complex was purified by metal ion affinity chromatography and characterized by analytical
65 rs, secreted into culture media, purified by affinity chromatography and characterized by biolayer in
66 n, as demonstrated by 1) binding assays (DNA affinity chromatography and ChIP) showing specific IGF-I
67 associate with human DNA polymerase delta by affinity chromatography and coimmunoprecipitation assays
68 nteraction assays of recombinant kinases and affinity chromatography and competition affinity assays.
70 To study resin fouling in situ, we coupled affinity chromatography and Fourier transform infrared (
71 essed in E. coli, purified by Ni-NTA agarose affinity chromatography and functionally characterized i
74 e-catecholic adducts, that were separated by affinity chromatography and high performance liquid chro
75 he crosslinking activity was purified by RNA affinity chromatography and identified as nucleolin by m
76 d by the inhibitory antibody was purified by affinity chromatography and identified by liquid chromat
80 enient purification by immobilized metal-ion affinity chromatography and labeling with [(99m)Tc(CO)3]
81 hese common alleles, we used oligonucleotide affinity chromatography and liquid chromatography-mass s
82 l transactivating factors by oligonucleotide affinity chromatography and mass spectrometry and confir
100 -enriched fractions, using immobilized metal affinity chromatography and tandem mass spectrometry.
102 Yersinia pseudotuberculosis were purified by affinity chromatography and used as immunogens to determ
103 3 proteins were not detected in the RNP, but affinity chromatography and yeast two-hybrid experiments
104 r derivatized to IMAC (immobilized metal ion affinity chromatography) and is investigated by loading
105 hia coli, purified in a one-step fashion via affinity chromatography, and activity as well as specifi
106 e enzyme-linked immunosorbent assay (ELISA), affinity chromatography, and bio-layer interferometry (B
107 expression, purified using immobilized metal affinity chromatography, and characterized for T cell bi
108 mogeneity by mutated streptavidin (SoftLink) affinity chromatography, and confirmed by mass spectrome
109 a pastoris, purified the proteins via tandem affinity chromatography, and determined MDR3-specific AT
110 ecipitation and nickel-nitrilotriacetic acid affinity chromatography, and further supported by co-loc
111 6 GRE and identify via UV cross-linking, RNA affinity chromatography, and mass spectrometry that is b
112 munoaffinity purification, immobilized metal affinity chromatography, and nanoflow liquid chromatogra
113 vels in Escherichia coli, purified by nickel affinity chromatography, and partially characterized.
114 tagenesis, coupled with splicing assays, RNA-affinity chromatography, and protein overexpression, and
115 tured in urea, purified by immobilized metal affinity chromatography, and renatured by a two-step dia
116 erum IgGs were subjected to standard antigen-affinity chromatography, and resulting elution, wash, an
119 purified from seeds, were separated by metal affinity chromatography, and their properties (GTPase ac
120 native gel electrophoresis, traditional and affinity chromatography assays and surface plasmon reson
121 When AUG3 was enriched from seedlings by affinity chromatography, AUG1 was detected by immunoblot
122 ly(dimethylsiloxane) (PDMS), implements cell-affinity chromatography based on the selective cell-capt
123 Two fractions of transferrin, separated by affinity chromatography based on their binding or not to
124 A simple, cheap, and highly reproducible affinity chromatography-based method has been developed
126 emonstrated for the first time how anti-IgG4 affinity chromatography can be used to prepare physiolog
127 easured using three primary assays (boronate-affinity chromatography, capillary isoelectric focusing
128 atographic phase to create cellular membrane affinity chromatography (CMAC) columns, CMAC(1321N1) and
129 ationary phase to form two cellular membrane affinity chromatography (CMAC) columns, CMAC(hOCT1(G465R
130 ey specifically interact, as demonstrated by affinity chromatography, co-immunoprecipitation, biosens
132 i-CD14, or anti-CD19 antibodies were used as affinity chromatography columns to separate target blood
134 ins from human U2OS osteosarcoma cells using affinity chromatography coupled to mass spectrometry emp
136 we describe the use of peptide aptamer-based affinity chromatography coupled with mass spectrometry t
139 tor (NF) 90 and NF45, as demonstrated by DNA affinity chromatography coupled with tandem mass spectro
141 xample, to fluorescent tags or 'handles' for affinity chromatography--directly on complex molecular s
142 ied a set of V-genes heavily enriched in the affinity chromatography elution, constituting the serum
143 Tryptic phosphopeptides (immobilized metal affinity chromatography-enriched) were identified and qu
151 ing partners for CaBP5 were identified using affinity chromatography followed by mass spectrometry an
152 d a comprehensive set of Drosophila Rabs for affinity chromatography followed by mass spectrometry to
153 of nonspecific binding in immobilized metal affinity chromatography for enrichment of phosphorylated
154 as the proteolytic enzyme, immobilized metal affinity chromatography for phosphopeptide enrichment, a
155 ocedure that combines membrane flotation and affinity chromatography for the purification of autophag
156 igned a metal-directed immobilized metal ion affinity chromatography for the sequential enrichment of
157 r region were purified by biotin-labeled DNA affinity chromatography from a large amount of host cell
158 '-UTR) binding proteins were purified by RNA affinity chromatography from cytosolic fractions of TNF-
159 The recombinant human Hsp72 was purified by affinity chromatography from insect cells, and purity wa
160 een isolated by origin sequence-specific DNA affinity chromatography from total chloroplast proteins.
161 (v)1.2 channels by CaBP5 were analyzed using affinity chromatography, gel overlay assays, and patch-c
163 system, named High Performance Multi-Lectin Affinity Chromatography (HP-MLAC), is composed of a seri
164 e rates involves the use of high-performance affinity chromatography (HPAC) and estimates of band-bro
168 ichment by TiO2 and Ti(4+)-immobilized metal affinity chromatography (IMAC) by a single operator.
170 C) employing gallium-based immobilized metal affinity chromatography (IMAC) in conjunction with titan
171 etry-compatible, iron(III)-immobilized metal affinity chromatography (IMAC) methods that use either n
172 ng phosphopeptides include immobilized metal affinity chromatography (IMAC) or titanium dioxide (TiO2
174 e goal of developing a new immobilized metal affinity chromatography (IMAC) stationary phase for sepa
175 e sequential Ga(3+)-Fe(3+)-immobilized metal affinity chromatography (IMAC) strategy displayed a 1.5-
179 5-/- embryos, using an Immobilized Metal-Ion Affinity Chromatography (IMAC), which specifically binds
180 TRAIL was accomplished by immobilized metal affinity chromatography (IMAC), which was followed by tr
182 ed by protective antibodies, we selected, by affinity chromatography, immunoglobulins against immobil
186 +/- 0.4 muM) and close to that determined by affinity chromatography in the literature (6 +/- 3 muM).
187 ins by immobilized metal ion and metal oxide affinity chromatography (in parallel and sequentially) f
188 n, we developed an approach that uses lectin affinity chromatography, ion-exchange chromatography, hy
193 ibody and protein A with in-line multilectin affinity chromatography (M-LAC) which consists of three
197 n IgG2 monoclonal antibody was studied using affinity chromatography, mass spectrometry, and chemical
198 ure in each candidate small molecule enables affinity chromatography-mass spectrometry, which produce
199 IBA) column as a viable small-molecule-based affinity chromatography method for antibody purification
206 All steps including immobilized metal ion affinity chromatography of protein on cobalt-loaded bead
209 ase-specific COMP fragments were isolated by affinity chromatography of synovial fluids from patients
212 ctin was purified from the coelomic fluid by affinity chromatography on a GlcNAc-derivatized column a
213 ilized broad-spectrum kinase inhibitors, (3) affinity chromatography on immobilized (R)-roscovitine a
215 Using ammonium sulphate precipitation and affinity chromatography on pepstatin-A agarose bed the a
217 ted proteins in the male genital tract using affinity chromatography on PSG2, an anti-sulfotyrosine m
218 (2)-dependent adhesion and migration; and 2) affinity chromatography on purified alpha(M)beta(2).
219 lin from serum, involving PEG precipitation, affinity chromatography on Sepharose derivatized with ac
221 n this paper, an open-tubular capillary cell affinity chromatography (OT-CAC) method to enrich and se
222 tion system with open-tubular capillary cell affinity chromatography (OT-CAC), although any separatio
224 ahistidines are very common tags used in the affinity chromatography purification of recombinant prot
226 dem Mass Spectrometry (LC-MS/MS) analysis of affinity chromatography purified antigens resulted in id
227 f heterologously expressed and metal-chelate-affinity chromatography-purified ZmEXPB6 on growth-reduc
228 industrial scale, replacing fouled Protein A affinity chromatography resin accounts for a large propo
229 r device requires only microliter volumes of affinity chromatography resin-a condition that maximizes
230 B was coated onto an IMAC (immobilized metal affinity chromatography) resin and successfully bound Co
231 is by similarity search, protein arrays, and affinity chromatography revealed four native ligand-rece
232 itochondrial membrane of T. brucei by tandem affinity chromatography revealed that TbTim17 associates
234 dem affinity purification vector followed by affinity chromatography, SDS-PAGE, and identification of
235 can be improved by application of a combined affinity chromatography shotgun immunoproteomic approach
239 tial elution protocol from immobilized metal affinity chromatography (SIMAC) employing gallium-based
240 Overexpressed SHI was purified by a single affinity chromatography step using the Strep-tag II, and
242 Optical methods include fluorescent frontal affinity chromatography, surface plasmon resonance metho
243 otein in mycobacterial species, and by using affinity chromatography techniques, we identify specific
245 enrichment by Ub-affinity and nickel chelate-affinity chromatography, the ubiquitylated proteins were
247 an alpha(2)beta(2)gamma(2) hexamer by nickel affinity chromatography through a "His(6)" affinity tag
248 capillary isoelectric focusing and boronate affinity chromatography to assess the formation and diss
249 m BsAbs that were purified through protein A affinity chromatography to demonstrate industrial applic
250 etry-based workflow that incorporates lectin affinity chromatography to enrich for proteins that carr
251 on of titanium dioxide and immobilized metal affinity chromatography to enrich phosphopeptides, we ex
252 ded protein was then re-isolated by means of affinity chromatography to identify and quantify the cap
257 which can be used in conjunction with avidin affinity chromatography to purify biotinylated peptides
258 ecombinant hSMVT in Pichia pastoris and used affinity chromatography to purify the detergent-solubili
259 geneous RNCs are isolated by combining metal affinity chromatography (to isolate ribosome-bound speci
261 in was purified from the bacterial lysate by affinity chromatography using a biotinylated promoter re
262 ly, the hydrazide product can be enriched by affinity chromatography using aldehyde resins, thus dras
263 ding to the SOSIP.664 design and purified by affinity chromatography using broadly neutralizing antib
264 b) was separated and quantitated by boronate affinity chromatography using optimized shielding reagen
265 phosphoethanolamine and could be purified by affinity chromatography using phosphoethanolamine-conjug
268 change chromatographies and then purified by affinity chromatography using Sepharose-immobilized kiwi
269 To identify binding factors, we conducted affinity chromatography using the A-box element and foun
270 targets in an unbiased fashion, we performed affinity chromatography, using tandem liquid chromatogra
271 ile Cic was not identified through our A-box affinity chromatography, utilization of the same site, t
276 tal proteomics approach involving dye-ligand affinity chromatography, we confirm NTP binding to 47 di
282 mple of the purification strategy via tandem affinity chromatography, we purified ABCC3 (MRP3) whose
285 expression in yeast and purification by CaM-affinity chromatography, we show that, unlike other ACAs
287 glycated materials, separated using boronate affinity chromatography, were fully characterized using
288 The columns were characterized using frontal affinity chromatography with [(3)H]-epibatidine as the m
289 step method to purify native-like trimers by affinity chromatography with a trimer-specific bNAb, PGT
290 either fluorescence spectrometry or boronate affinity chromatography with detection by specific fluor
291 c acid (NMDA), was established using frontal affinity chromatography with flunitrazepam and diazepam
295 in more weakly than wild-type as observed by affinity chromatography with scaffolding protein-agarose
297 l antibodies by combining the selectivity of affinity chromatography with the simplicity of salt-indu
298 n of partially assembled F(O)F1 complexes by affinity chromatography with the use of mutants synthesi
299 action of CaBP4 and Unc119 was studied using affinity chromatography, yeast two-hybrid system, coimmu
300 the precursor protein, followed by negative affinity chromatography, yielded a purified peptide.
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