ライフサイエンスコーパス: affinity label

1 , we used 4'-azido-warfarin-3H-alcohol as an affinity label.

2 e and in determining the specificity of this affinity label.

3 re and well-positioned for reaction with the affinity label.

4 n of small peptide substrates into potential affinity labels.

5 osol and nuclei using fluorogenic assays and affinity labeling.

6 erved residues, Y230 and W232, important for affinity labeling.

7 Glu-68 as the only residue to participate in affinity labeling.

8 telomerase RNA (TER) have been localized by affinity labelling.

9 coli enzyme has been investigated using the affinity label [(14)C]-p-fluorosulfonylbenzoyl adenosine

10 on is reinforced by the observation that the affinity label, 2-butynoyl-CoA, stoichiometrically modif

11 zyme, are stoichiometrically modified by the affinity label, 2-butynoyl-CoA.

12 Previous studies used the photoreactive affinity label 3-[3H]azido-2-methyl-5-methoxy-6-geranyl-

13 inhibition studies with the substrate-based affinity label 3-bromopyruvate (3-BP).

14 he basis of the X-ray structures of free and affinity-labeled 4-OT.

15 ainst IAP specifically immunoprecipitate the affinity-labeled 52-kDa protein from lysates of IAP-posi

16 absence of NAD), and of inactivation by the affinity label 6-chloro-purine-ribotide (6-Cl-PRT).

17 d on the sequence RFYVVM from the CBD of TS1 affinity label a 52-kDa cell surface glycoprotein, which

18 n alkylating derivative, quinacrine mustard, affinity labeled a subset of the substituted cysteines i

19 A photoreactive peptide is synthesized that affinity-labels a single site in the antibody variable d

20 F) both inhibited heat-induced apoptosis and affinity-labeled activated initiator caspase-2, -8, and

21 ytometry, immunoblotting, enzyme assays, and affinity labeling after treatment with EGF, paclitaxel,

22 Titrating C161 of LRAT with a specific affinity labeling agent at varying pH values shows that

23 inally, 11-cis-retinyl-bromoacetate, a known affinity-labeling agent of isomerohydrolase, also blocks

24 Potent and specific chloroketone containing affinity labeling agents have been developed.

25 end, chemical probes that incorporate known affinity labeling agents have facilitated the activity-b

26 y labeled with all-trans-retinyl ester based affinity labeling agents, suggesting a retinyl ester bin

27 We have examined the effect that an affinity-label analog of the synergistic anion has on ch

28 125I-TGF-beta1 affinity labeling analysis of cell-surface TGF-beta rece

29 The combined utilization of a reporter affinity label and site-directed mutagenesis offers a mo

30 Using both affinity labeling and fluorescence quenching, we have ob

31 Affinity labeling and geometric constraints ensure that

32 ty of the enzyme is exceedingly sensitive to affinity labeling and group-specific reagents directed t

33 Affinity labeling and immunochemical studies characteriz

34 Photo-affinity labeling and mutagenesis studies have identifie

35 identified Asp-23 through the procedures of affinity labeling and site-directed mutagenesis as a cat

36 er membrane protein detected by folate-based affinity labeling and with anti-mouse RFC-1 peptide anti

37 From affinity labelling and mutagenesis studies, the ANP-bind

38 e kinetic parameters of active-site-directed affinity labels and have also been applied to a few suic

39 ing these protocols together with well known affinity labels and mechanism-based inactivators should

40 determined by surface protein biotinylation, affinity labeling, and immunoelectron microscopy studies

41 alytic features of FAAH through mutagenesis, affinity labeling, and steady-state kinetic methods.

42 Results show that when the affinity-label anion is utilized, strictly hyperbolic da

43 cturally different antagonists including the affinity labeling antiglucocorticoid dexamethasone 21-me

44 Our synthetic receptor-mediated affinity labeling approach broadens the scope of PTM det

45 cements for His141, previously identified by affinity labeling as being in the active site.

46 s of naphthalene dicarboxaldehyde-containing affinity labels as kinetic probes.

47 and the other by a more discrete and higher affinity labeling at Trp134-Gly135.

48 ng-site directed analogs of 1,25(OH)2D3, and affinity-labeled baculovirus-expressed recombinant human

49 -Asp192 and gamma-Asp190 had previously been affinity labeled by a reactive ADP analogue and alpha-As

50 Finally, the pH dependence of kinact/KI for affinity labeling by 3-BP yields a pKa value of 6.7 +/-

51 ach reverses the paradigm of ligand-directed affinity labeling by making the receptor the synthetic c

52 1965 region of domain IV, around the site of affinity labeling by the 3' end of tRNA, and the second

53 Seprase could be affinity-labeled by [3H]diisopropyl fluorophosphate, but

54 The approach of affinity labeling can be used to identify amino acid par

55 Immunoanalysis of affinity labeled caspases demonstrated that caspase-3 wa

56 ment and subsequent affinity purification of affinity-labeled caspases confirmed that at least three

57 Here, we report a general strategy in using affinity-labeled chemical probes to enrich, identify, an

58 -1-BE and [3H]-1alpha,25(OH)2D3-1,3-di-BE to affinity label DBP.

59 The affinity labels did not bind to receptors known to effec

60 active site in the crystal structure of the affinity-labeled enzyme, Arg-61 does not play a signific

61 te tautomerase in the X-ray structure of the affinity-labeled enzyme.

62 inding to analogues of substrates and ATP in affinity labeling experiments and displayed a high level

63 lop a reliable model based on the results of affinity labeling experiments that provide atomic coordi

64 Affinity labeling experiments using specific compounds w

65 Affinity labeling experiments, using either [17-3H]10 or

66 Therefore, this peptide is a potent affinity label for further study of delta opioid recepto

67 ntAdo may also prove useful as a fluorescent affinity label for other ATP binding sites.

68 on these results, we have designed a potent affinity label for PKG (KI = 21.1 +/- 4.7 microM), that

69 ctive active site-directed irreversible cAMP affinity label for platelet PDE3A and can be used to ide

70 e studies show that FSBMantAdo is a specific affinity label for the ATP site of the EGF receptor.

71 l)adenosine (5'-FSBAdo) is a highly specific affinity label for the ATP site of the kinase domain of

72 that 2-methyl-4-bromopyridine is a quiescent affinity label for the nitric oxide controlling enzyme d

73 iloyl)adenosine (FSBMantAdo), as fluorescent affinity labels for adenine nucleotide binding sites, an

74 To develop affinity labels for delta opioid receptors based on pept

75 Phe(3) or Phe(4) were prepared as potential affinity labels for delta-opioid receptors.

76 tides are the highest affinity peptide-based affinity labels for MOR reported to date.

77 c procedure which allows for introduction of affinity labeling groups late in the synthesis of a vari

78 r of the ICE/CED-3 family is demonstrated by affinity-labeling GT1-7 extracts from apoptotic controls

79 A vitamin A containing affinity-labeling haloacetate is described which facilit

80 ilized mutagenesis, receptor chimeras, photo-affinity labeling, hydrogen-deuterium exchange, and crys

81 A series of potent electrophilic affinity labels (IC(50) = 0.1-5 nM) containing either a

82 he IL-15R utilized covalent cross-linking to affinity label IL-2R and IL-15R on YT cells and OKT3 bla

83 Additionally, a designed affinity label inhibits LpxC in a time-dependent manner.

84 cleobase derivatives bearing fluorophores or affinity labels into a short RNA stem loop recognition m

85 Affinity labeling is a powerful tool to establish spatia

86 High-affinity labeling is demonstrated by covalent attachment

87 and-binding domain of AR by replacing a high-affinity labeled ligand (IC(50) 0.4 muM).

88 g domain within this enzyme, we employed the affinity labeled long-chain fatty acid [(3)H]9-p-azidoph

89 p-amine group in the peptides to the desired affinity labeling moieties.

90 an isothiocyanate group as the electrophilic affinity labeling moiety.

91 um [3H]borohydride shows that 1.2 mol of the affinity label/mol of enzyme was incorporated.

92 The combined approach of photo-affinity labeling/MS analysis with site-directed mutagen

93 s study, covalent modification of CS2 by the affinity label N-bromoacetyl-L-arginine near His297, whi

94 The first example of the use of a reporter affinity label (NNA) that contains a fluorogenic naphtha

95 These results indicate that TIA reacts as an affinity label of a distinguishable tocopherol binding s

96 BITC also acts as an affinity label of hGST P1-1 in the absence of glutathion

97 Bromopyruvate, a known synergistic anion and affinity label of ovotransferrin (oTF), was used to prob

98 auxin indole-3-acetic acid, functions as an affinity label of the dimeric pi class glutathione S-tra

99 iodoacetoxy-estradiol-3-sulfate reacts as an affinity label of the nonsubstrate steroid site rather t

100 Affinity labeling of FAAH with a specific nucleophile re

101 This is the first identification by affinity labeling of non-reactive amino acids within the

102 These data are consistent with affinity labeling of other members of the class B G prot

103 ion spectroscopy (FCS), we demonstrated high-affinity labeling of the active receptor (R*) conformati

104 -CoA binding domain of FadR was localized by affinity labeling of the full-length protein and an amin

105 sed; displacement ligand binding studies and affinity labeling of the IGF-1R alpha subunit indicated

106 Affinity labeling of the protease activity with an irrev

107 The 125I-IGFBP-3 affinity labeling of the putative receptor and IGFBP-3-i

108 ding the previously most informative site of affinity labeling of this receptor.

109 nylated inactivator peptides are active-site affinity labels of PKC.

110 The 52-kDa protein is affinity labeled on IAP-positive but not IAP-negative ce

111 t of mature absorptive cells with either the affinity label or the anti-RFC-1 peptide antibodies inhi

112 type V TGF-beta receptor after 125I-TGF-beta affinity labeling or Trans35S-label metabolic labeling o

113 degradation are temporally linked, and pulse affinity-labeled p29 is internalized and sequestered in

114 ith a specific anti-p29 IgG shows that pulse affinity-labeled p29 reappears on the plasma membrane ap

115 merizable small molecule, or photoswitchable affinity label (PAL), that specifically targets K+ chann

116 Affinity labels patterned in part after the potent antie

117 ptides generated by tryptic digestion of the affinity-labeled proteins identified one peptide common

118 Using a modification of a highly selective affinity labeling protocol, we demonstrated that the alp

119 that the approximately 400-kDa 125I-IGFBP-3 affinity-labeled putative IGFBP-3 receptor is immunoprec

120 The 125I-IGFBP-3 affinity-labeled putative receptor can only be detected

121 Three affinity labels, (R,S)-N-benzyl-2-acetamido-6-isothiocya

122 The affinity labeling reagent, 3'-O-(5-fluoro-2,4-dinitrophe

123 t both Arg131 and Arg303 are modified by the affinity-labeling reagent.

124 rambucil have been used as sequence-directed affinity labeling reagents to investigate the length and

125 ults in partial cleavage of the enzyme-bound affinity label, restoration of enzymatic activity (60-80

126 In this article, our affinity labeling results present the first evidence tha

127 ar selectivity was observed in intact cells, affinity labeling revealed that the active caspase speci

128 rate protection to validate specificity, the affinity labeling secosteroid has identified peptides in

129 ther by X-ray or by NMR, inactivation by two affinity labels showed half-site stoichiometry, and titr

130 t the first report of tandem applications of affinity labeling, site-directed mutagenesis, and intera

131 ctural integrity based on Mn(2+) binding and affinity labeling stoichiometry.

132 A double affinity-labeling strategy was used to isolate dimers th

133 Affinity labeling studies revealed that, unlike the mamm

134 role of Pro-1 in the MIF-catalyzed reaction, affinity labeling studies were performed with 3-bromopyr

135 structure-activity relationship studies, and affinity labeling studies.

136 Results of affinity-labeling studies and mutational analyses provid

137 stagnalis and the results from site-directed affinity-labeling studies were used as the basis for mod

138 A recent affinity labeling study has suggested that amino acids 7

139 In addition, 125I-IGFBP-3 affinity-labeled TbetaR-V in Mv1Lu cells is immunoprecip

140 Affinity labeling techniques revealed that RII-transfect

141 ocannabinol (AM841), a classical cannabinoid affinity label that incorporates an isothiocyanate subst

142 2) and [Phe(p-NCS)(4)]TIPP (4) represent two affinity labels that may prove useful in the study of de

143 y available reagents are used to selectively affinity label the alpha-amine that characterizes the pr

144 25-OH-D3-3-BE and [3H]-1alpha,25(OH)2D3-3-BE affinity labeled the protein.

145 rent work, we have developed a new probe for affinity labeling the secretin receptor through a photol

146 ect on CCK binding, stimulated signaling, or affinity labeling through a residue within the pharmacop

147 In this method nuclei are affinity-labeled through transgenic expression of a biot

148 d ADP analog, has been previously used as an affinity label to examine the structure/function relatio

149 wledge, 2 represents the first peptide-based affinity label to utilize an isothiocyanate group as the

150 The aim of this work was to utilize affinity labeling to determine spatial approximations wi

151 ificities, immunoevasins can be exploited as affinity labels to identify host-encoded molecules of pr

152 o)glucan oligosaccharides and an active-site affinity label, together with detailed kinetic analysis

153 The LC/MS/MS analysis of the affinity-labeled tryptic peptides purified from HPLC, id

154 atizes the larger subunit of active ICE, can affinity label up to five active IRPs in S/M extracts.

155 enkephalin analogue 2 emerges as a potential affinity label useful for the further study of delta opi

156 An active site-directed peptide-based affinity label was designed, synthesized, and employed t

157 Furthermore, the affinity labeling was UV-dependent and saturable, indica

158 cs probes for enzyme classes lacking cognate affinity labels, we describe here a combinatorial strate

159 npeptide opioid agonists and antagonists and affinity labels were fit into the binding pockets of the

160 tructure and antigenicity in acrylic resins, affinity labels were repeatedly applied, imaged, removed

161 l isomerase domain (previously identified by affinity labeling) were created, expressed, and purified

162 Mutation of C288 to glycine abolished this affinity labeling, whereas the VDR-LBD mutants C337G and

163 arboxyl terminal, assembles with SUR1 and is affinity labeled, while deletion of 10 or more amino-ter

164 ltered spectrum of active caspases that were affinity labeled with N-(Nalpha-benzyloxycarbonylglutamy

165 entification of the protein was confirmed by affinity labeling with 14CN- and isoelectrofocusing.

166 Affinity labeling with 2-(14)C-labeled bromopyruvate ind

167 Affinity labeling with [(14)C]CRIDs and affinity chromat

168 se activity was identified as cathepsin L by affinity labeling with an activity-based probe for cyste

169 Affinity labeling with biotin-VAD-fmk of all active casp

170 Likewise, affinity labeling with N-(N(alpha)-benzyloxycarbonylglut

171 racts containing active caspases followed by affinity labeling with N-(N-benzyloxycarbonylglutamyl-N-

172 ing strategy, which integrates site-directed affinity labeling with structure-activity knowledge to c

173 red membrane currents before and after photo-affinity labeling with the agonist 2'(3')-O-(4-benzoylbe

174 Inositol deacylase was affinity labelled with [3H]DFP and purified.

175 fold and identified as a serine hydrolase by affinity labelling with a biotinylated fluorophosphonate

176 The chemical reactivity of the affinity labels with nucleophiles was assessed, and the

177 f the alphaB crystallin Walker-B motif photo-affinity-labeled with azido-ATP-EDANS confirming the bet

178 and C-prodomain (amino acids 1000-1453) was affinity-labeled with human placental alkaline phosphata

179 t generated by cathepsin D cleavage could be affinity-labeled with the active site probe biotin-VAD-f