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1 erved residues, Y230 and W232, important for affinity labeling.
2 Glu-68 as the only residue to participate in affinity labeling.
3 osol and nuclei using fluorogenic assays and affinity labeling.
4 ytometry, immunoblotting, enzyme assays, and affinity labeling after treatment with EGF, paclitaxel,
6 inally, 11-cis-retinyl-bromoacetate, a known affinity-labeling agent of isomerohydrolase, also blocks
8 end, chemical probes that incorporate known affinity labeling agents have facilitated the activity-b
9 y labeled with all-trans-retinyl ester based affinity labeling agents, suggesting a retinyl ester bin
13 ty of the enzyme is exceedingly sensitive to affinity labeling and group-specific reagents directed t
16 identified Asp-23 through the procedures of affinity labeling and site-directed mutagenesis as a cat
17 er membrane protein detected by folate-based affinity labeling and with anti-mouse RFC-1 peptide anti
18 determined by surface protein biotinylation, affinity labeling, and immunoelectron microscopy studies
19 alytic features of FAAH through mutagenesis, affinity labeling, and steady-state kinetic methods.
20 cturally different antagonists including the affinity labeling antiglucocorticoid dexamethasone 21-me
24 Finally, the pH dependence of kinact/KI for affinity labeling by 3-BP yields a pKa value of 6.7 +/-
25 ach reverses the paradigm of ligand-directed affinity labeling by making the receptor the synthetic c
26 1965 region of domain IV, around the site of affinity labeling by the 3' end of tRNA, and the second
28 inding to analogues of substrates and ATP in affinity labeling experiments and displayed a high level
29 lop a reliable model based on the results of affinity labeling experiments that provide atomic coordi
32 c procedure which allows for introduction of affinity labeling groups late in the synthesis of a vari
33 r of the ICE/CED-3 family is demonstrated by affinity-labeling GT1-7 extracts from apoptotic controls
35 ilized mutagenesis, receptor chimeras, photo-affinity labeling, hydrogen-deuterium exchange, and crys
44 ion spectroscopy (FCS), we demonstrated high-affinity labeling of the active receptor (R*) conformati
45 -CoA binding domain of FadR was localized by affinity labeling of the full-length protein and an amin
46 sed; displacement ligand binding studies and affinity labeling of the IGF-1R alpha subunit indicated
50 type V TGF-beta receptor after 125I-TGF-beta affinity labeling or Trans35S-label metabolic labeling o
51 Using a modification of a highly selective affinity labeling protocol, we demonstrated that the alp
54 rambucil have been used as sequence-directed affinity labeling reagents to investigate the length and
56 ar selectivity was observed in intact cells, affinity labeling revealed that the active caspase speci
57 rate protection to validate specificity, the affinity labeling secosteroid has identified peptides in
58 t the first report of tandem applications of affinity labeling, site-directed mutagenesis, and intera
62 role of Pro-1 in the MIF-catalyzed reaction, affinity labeling studies were performed with 3-bromopyr
65 stagnalis and the results from site-directed affinity-labeling studies were used as the basis for mod
68 rent work, we have developed a new probe for affinity labeling the secretin receptor through a photol
69 ect on CCK binding, stimulated signaling, or affinity labeling through a residue within the pharmacop
72 l isomerase domain (previously identified by affinity labeling) were created, expressed, and purified
73 Mutation of C288 to glycine abolished this affinity labeling, whereas the VDR-LBD mutants C337G and
74 entification of the protein was confirmed by affinity labeling with 14CN- and isoelectrofocusing.
77 se activity was identified as cathepsin L by affinity labeling with an activity-based probe for cyste
80 racts containing active caspases followed by affinity labeling with N-(N-benzyloxycarbonylglutamyl-N-
81 ing strategy, which integrates site-directed affinity labeling with structure-activity knowledge to c
82 red membrane currents before and after photo-affinity labeling with the agonist 2'(3')-O-(4-benzoylbe
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