ライフサイエンスコーパス: agarose

1 0.5 mug (for polyacrylamide) to 2.5 mug (for agarose).

2 be mechanically robust when inserted into 2% agarose.

3 he aldehyde-modified protein using hydrazide-agarose.

4 ty-purified using human beta(2)-GP1 bound to agarose.

5 onventional aqueous gels such as gelatin and agarose.

6 ous growth factors or form large colonies in agarose.

7 ptides are affinity-captured on streptavidin-agarose.

8 onto an activated support of glutaraldehyde agarose.

9 specimen from a pin or embedding it in 1-2% agarose.

10 ties to hydrolyze the glycosidic linkages in agarose, a linear polymer comprising the repeating disac

11 ional sites on ion-exchange ligands based on agarose, a support matrix routinely used in protein chro

12 was expressed in E. coli, purified by Ni-NTA agarose affinity chromatography and functionally charact

13 d directly to PrPC was demonstrated by hemin-agarose affinity chromatography and UV-visible spectrosc

14 pressed in E. coli, and purified with Ni-NTA agarose affinity chromatography.

15 rface proteins were isolated by streptavidin agarose affinity purification and then separated by elec

16 rface proteins were isolated by streptavidin-agarose affinity purification.

17 g viable and functional pancreatic islets in agarose-agarose macrobeads (diameter 6-8 mm) might provi

18 orporated into two different hydrogel films, agarose and a copolymer of acrylamide and 2-(dimethylami

19 ucleic acid probes embedded within permeable agarose and alginate hydrogels.

20 Adenosine triphosphate-agarose and calmodulin-agarose pull-down assays show tha

21 carbohydrate but not related substrates like agarose and carrageenan.

22 inase pathway results in slow movement under agarose and cells that produce few blebs, though actin p

23 hly sensitive toward carbohydrates-on native agarose and chemically modified agarose in the gel phase

24 The film of blended agarose and chitosan allows good dispersion of C18, prev

25 KHDC1A and 1B bind polyU agarose and form oligomers like other KH-domain proteins

26 Agarose and gelatin form non-interactive bicontinuous ph

27 re then affinity-purified using streptavidin-agarose and identified by multi-dimensional protein iden

28 using nickel-nitrilotriacetic acid (Ni-NTA) agarose and incubate the immobilized TFs with fluorescen

29 Hybrid films of agarose and lipids even enabled the formation of giant l

30 Hydrating the films of agarose and lipids in aqueous salt solutions resulted in

31 tion of giant liposomes from hybrid films of agarose and lipids in comparison to established methods

32 ows that gentle hydration of hybrid films of agarose and lipids is a simple, rapid, and reproducible

33 oceeded from hybrid films of partially dried agarose and lipids.

34 UVs spontaneously swell from hybrid films of agarose and lipids.

35 n optimized to migrate appropriately on both agarose and native polyacrylamide, unlike many currently

36 d degradation fragments were quantified with agarose and polyacrylamide gel electrophoresis and immun

37 Agarose and polyacrylamide gel electrophoresis systems f

38 Most of the GIP were retained by the G12-agarose and represented the largest part of the immunoge

39 nity chromatography with scaffolding protein-agarose and scaffolding protein shell re-entry experimen

40 ons have on the selective adsorption between agarose and SWCNTs suspended with sodium dodecyl sulfate

41 otin-N-maleimide, purified with streptavidin-agarose, and resolved by 2-dimensional gel electrophores

42 ized biocatalyst was developed using glyoxyl-agarose as support; it exhibited high performance in deg

43 dded fluorescent microplastics in artificial agarose-based food and offered the food to marine isopod

44 The agarose-based gels were "doped" with sodium poly(styrene

45 For agarose-based systems, the suitability of different agar

46 with prevascularization of the graft bed by agarose-basic fibroblast growth factor.

47 gest that, due to the porous interior of the agarose bead, internal analyte transport is both diffusi

48 CypD(-/-) mice to immunoprecipitation using agarose beads coupled to antiacetyl lysine antibodies fo

49 Monocytes migrating toward agarose beads impregnated with conditioned media from M.

50 led to nickel nitrilotriacetic acid (Ni-NTA) agarose beads, specifically recruited F-actin in the pre

51 was determined by pull-down assays with PAK-agarose beads.

52 mouse GPIHBP1 and used that antibody to coat agarose beads.

53 teraction using metabolite immobilization on agarose beads.

54 g cells with how the same process unfolds on agarose beads.

55 n and affinity chromatography on pepstatin-A agarose bed the aspartyl proteases were purified and con

56 as immobilized on sodium alginate as well as agarose bed.

57 mutant PTEN to bind ATP was assessed by ATP-agarose-binding assays.

58 e procedure of introducing gaps by digesting agarose blocks imbedded in the cell sheets.

59 talyzes a critical step in the metabolism of agarose by S. degradans through cleaving agarose oligome

60 Heme agarose captured ZnuD in enriched outer membrane fractio

61 ing muscle movements by embedding embryos in agarose caused similar defects in peripheral RB axon gui

62 Agarose-chitosan-immobilized octadecylsilyl-silica (C18)

63 ed from murine serum by gelatin cross-linked agarose chromatography and subsequently was enzymaticall

64 ysophosphatidyl glycerol (LPG) by Ni(2+)-NTA agarose chromatography to >95% purity with high yield.

65 he flow-through fraction upon subsequent ATP-agarose chromatography.

66 antithrombin that are resolvable by heparin-agarose chromatography.

67 Cells were grown in agarose-coated 96 well plates, forming reproducible 1-mm

68 NA (ssDNA) probe sequence was immobilized on agarose-coated magnetic beads, that in turn can be local

69 d the deposition of complement components on agarose-coated plates, although it could not remove prev

70 separated on a nickel-nitrilotriacetic acid-agarose column based on the number of His tags present i

71 Escherichia coli BL21 and purified by Ni-NTA agarose column.

72 on agarose was also found equal to 120 s at agarose concentration of 1.2% (w/v) and optical density

73 volumes were used, but was not influenced by agarose concentration or the presence of ethidium bromid

74 the range of 1:170 to 1:3100 for beads with agarose concentration ranging from 0.5% to 8% for the se

75 the suitability of different agarose types, agarose concentrations, and buffer systems was determine

76 the passive diffusion response of dextran in agarose confirms the applicability of Fick's law of diff

77 Chondrocyte-agarose cultures with well-established extracellular mat

78 In porcine chondrocyte-agarose cultures, a 37-kd ADAMTS-4 isoform appears to be

79 cellular matrix formed by 3-week chondrocyte-agarose cultures.

80 e developed and tested a method using hollow agarose cylinders designed to accommodate for embryonic

81 The mechanical properties of agarose-derived hydrogels depend on the scaffolding of t

82 Diffusion coefficients (D) through agarose diffusive gels ranged from (1.02 to 4.74) x 10(-

83 to loading using a model system of acellular agarose disks and dextran in phosphate-buffered saline (

84 ntly enhanced the rate of solute uptake into agarose disks, relative to static loading.

85 Edges that are bordered by agarose do not induce activation of the EGFR, indicating

86 o-processive mode of action of Aga50D on the agarose double helix.

87 onsequently, the permanent dipole moments of agarose drastically reduces the retention of SWCNTs.

88 d due to substantial release of encapsulated agarose during temperature treatment.

89 We report that crosslinked agarose (e.g., Sepharose) chromatography medium that has

90 we developed a single-cell protocol based on agarose-embedded bisulfite treatment, which allows inves

91 -dependent intracellular Ca(2+) signaling in agarose-embedded chondrocytes, and then used this model

92 rometer-resolution 3D images of paraffin- or agarose-embedded whole organs with high fidelity, achiev

93 including how to hold mouse embryos without agarose embedding, how to transfer embryos without air e

94 ased on a mixture of neutral, coarse fibers (agarose fibrils), and fine, charged fibers (GAG chains).

95 Further fractionation of the ATP-agarose flow-through on Sephacryl S-300 separates free t

96 are heated above the melting temperature of agarose for 2 h before use, vesicle response is (partial

97 h as ribosomal RNAs and their precursors, on agarose-formaldehyde gels.

98 lowed down for agarose-GUVs when compared to agarose-free GUVs.

99 replication models based on two-dimensional agarose gel analyses.

100 was assessed by Western blotting and native agarose gel analysis in Huh7 cells, and the human immune

101 have been experimentally validated by QPCR, agarose gel analysis, sequencing and BLAST, and all vali

102 rk integrity as monitored by two-dimensional agarose gel analysis.

103 205 squamous carcinoma cells embedded in an agarose gel and cell spheroids in Matrigel.

104 determined from 32 phantoms constructed with agarose gel and in eight concentrations from each of the

105 Ligation was verified by agarose gel and small-angle X-ray scattering.

106 The same irradiated samples were analyzed by agarose gel and SSB yields were determined by convention

107 tilting angle of the microtubules buried in agarose gel and to find the precise surface plasmon reso

108 10-, 20-, and 30-mmol/L NaCl solution and 5% agarose gel as a reference.

109 MVm and C5a) compared with WT using an under-agarose gel chemotaxis assay.

110 In this protocol, droplets of agarose gel containing different chemokines are applied

111 s ever observed for compression of uncharged agarose gel controls.

112 present work proposes the improvement of an agarose gel DNA electrophoresis in order to allow for a

113 describe the development of 2D intact mtDNA agarose gel electrophoresis (2D-IMAGE) for the separatio

114 eosomal arrays were determined by analytical agarose gel electrophoresis (AAGE) and single molecules

115 d in vitro using a low-temperature EDTA-free agarose gel electrophoresis (LTEAGE) procedure.

116 block architectures were characterized by 1% agarose gel electrophoresis and atomic force microscope

117 We used two-dimensional agarose gel electrophoresis and electron microscopy to a

118 The quantity and quality were confirmed by agarose gel electrophoresis and polymerase chain reactio

119 y a dye-doped silica shell were separated by agarose gel electrophoresis and scanned by a conventiona

120 urse of infection by one and two-dimensional agarose gel electrophoresis and Southern hybridization.

121 ucts (e.g., open circle and linear forms) by agarose gel electrophoresis and subsequently quantified

122 Agarose gel electrophoresis and ultraviolet spectrophoto

123 reaction (PCR) are exploited using on-column agarose gel electrophoresis as separation and inductivel

124 By adopting a native agarose gel electrophoresis assay that can specifically

125 capsids that migrated more slowly in native agarose gel electrophoresis from A36V mutant than from t

126 In the current work, an improved agarose gel electrophoresis method for analysis of high

127 The widely used agarose gel electrophoresis method for assessing radiati

128 Agarose gel electrophoresis of DNA and RNA is routinely

129 minimize errors and is broadly applicable to agarose gel electrophoresis of RNA samples and their sub

130 ild-type and mutant plants by single-nucleus agarose gel electrophoresis revealed that bleomycin-indu

131 Two-dimensional agarose gel electrophoresis revealed that ORC2 depletion

132 We verified these AFM results by agarose gel electrophoresis separation of UV-irradiated

133 Agarose gel electrophoresis showed that high molecular w

134 We show by two-dimensional (2D) agarose gel electrophoresis that replication forks natur

135 genetics and high resolution two-dimensional agarose gel electrophoresis to examine the torsional ten

136 We apply agarose gel electrophoresis to sensitively evaluate prot

137 Agarose gel electrophoresis was subsequently used to sep

138 Using two-dimensional agarose gel electrophoresis we also show that UvsW acts

139 PCR amplification, agarose gel electrophoresis, and sequencing methods were

140 Agarose gel electrophoresis, circular dichroism and diff

141 are based on two-dimensional, non-denaturing agarose gel electrophoresis, followed by structure deter

142 Here, by native agarose gel electrophoresis, using recombinant IN with a

143 Using two-dimensional agarose gel electrophoresis, we examined DNA replication

144 Using one- and two-dimensional agarose gel electrophoresis, we show that the linear mtD

145 gher sensitivity as compared to conventional agarose gel electrophoresis.

146 ng transmission electron microscopy and 1.2% agarose gel electrophoresis.

147 ugation, and retarded mobility during native agarose gel electrophoresis.

148 wed by amplifications with ISSR and SCoT and agarose gel electrophoresis.

149 when positive, and results were verified by agarose gel electrophoresis.

150 ed, as revealed by semi-denaturing detergent agarose gel electrophoresis.

151 d transfer complex can be isolated by native agarose gel electrophoresis.

152 generate monovalent quantum dots (QDs) using agarose gel electrophoresis.

153 by bovine pancreatic trypsin immobilised on agarose gel in 100 mM ammonium hydrocarbonate buffer, pH

154 %) and without contaminants such as residual agarose gel or DNA intercalating dyes.

155 The computational results are validated with agarose gel phantom experiments.

156 mical laser-induced nucleation of an aqueous agarose gel prepared with supersaturated potassium chlor

157 ted by the electrophoresis in polyacrylamide/agarose gel profile.

158 s measured, and the results suggest that the agarose gel reduces the effective supersaturation of the

159 ent pH changes throughout a nanowire network/agarose gel sample during external solution pH changes,

160 were separated by electrophoresis on a 0.8% agarose gel stained with ethidium bromide.

161 lts demonstrate that any modification to the agarose gel surface and, consequently, the permanent dip

162 cted a new nucleoprotein complex on a native agarose gel that was produced in the presence of >200 nM

163 that saccharides systematically decrease the agarose gel thinning rate up to a factor two, and exempl

164 domly distributed fluorescent nanospheres in agarose gel were obtained and fitted with the theoretica

165 al diameter, 0.579 mm) shallowly embedded in agarose gel, then to a collection reservoir.

166 ision and integration, we first developed an agarose gel-based assay for CTnDOT recombination, which

167 s as easy, convenient, and inexpensive as an agarose gel.

168 digestion, and detection of fragments on an agarose gel.

169 ass substrate while they are diffusing in an agarose gel.

170 rcoiled (sc) form of plasmid DNA (pDNA) from agarose gel.

171 ering was evaluated by electrophoresis on 3% agarose gel.

172 lso used to extract the sc form of pDNA from agarose gel.

173 as quantitated by radial diffusion in fibrin-agarose gel.

174 .22 in solution to 1.2 +/- 0.04 in a 3 w/v % agarose gel.

175 bilayer is sandwiched between two layers of agarose gel.

176 native conditions through polyacrylamide or agarose gel.

177 ere analyzed by electrophoresis performed on agarose gel; samples with a discrete or localized band w

178 purifying DNA-origami nanostructures rely on agarose-gel electrophoresis (AGE) for separation.

179 e-specific PCR qualitative assay followed by agarose-gel electrophoresis.

180 ing the uptake of (45)Ca by isolated ACVs in agarose gels and by ACVs in situ in freeze-thawed cartil

181 migrated with undigested parental capsids on agarose gels and cosedimented with undigested capsids by

182 Agarose gels are viscoelastic soft solids that display a

183 Using carboxylated agarose gels as a screening platform, we demonstrate tha

184 trap the synaptic complex observed on native agarose gels correlated with its potency for inhibiting

185 Selective adsorption onto agarose gels has become a powerful method to separate si

186 ing kinetics and compare the drying speed of agarose gels loaded with various non-gelling saccharides

187 ings imply that the SSB yields inferred from agarose gels need reevaluation, especially when they wer

188 tall the extension of the acrosome bundle in agarose gels of different concentrations.

189 tudy by in-situ interferometry the drying of agarose gels of various compositions cast in Petri dishe

190 technique employs topographically patterned agarose gels to deliver various membrane preparations to

191 sensors in conjunction with collagen-coupled agarose gels to detect subcellular activities of SFK and

192 ection of 10 muL cell inclusions in cm-sized agarose gels used here as phantom models of microtumors.

193 To probe that question, U(IV) immobilized in agarose gels was exposed to conditions allowing biologic

194 Heterogeneity within agarose gels was modeled by assuming that fiber-rich, sp

195 We use micropatterned, enzyme-laden agarose gels which are stamped on polyacrylamide films c

196 els, reconstituted basement membrane matrix, agarose gels, alginate gels, and fibrin gels, but not in

197 d (female) were isolated, electrophoresed in agarose gels, and transferred to nylon membranes.

198 or 12 h, separated by electrophoresis on 2 % agarose gels, and visualized with ethidium bromide stain

199 de fluorescence of PCR products excised from agarose gels.

200 ctrophoretic transport of lambda-DNA through agarose gels.

201 DNA that can be visualized in vivo using 2D agarose gels.

202 etected as the corresponding PCR amplicon in agarose gels.

203 ecular mass as low as approximately 9 kDa in agarose gels.

204 breast cancer cells seeded into nonadhesive agarose gels.

205 ion in the Darcy permeability of 3 vol/vol % agarose gels.

206 Titin isoform expression was evaluated with agarose gels.

207 orescently labeled flagellar filaments at an agarose-glass interface.

208 Agarose-glycosaminoglycan (GAG) membranes were synthesiz

209 n be purified using Galanthis nivalis lectin agarose (GNA), but this technique is suboptimal for glob

210 lasts could no longer form large colonies in agarose, grow in reduced levels of serum, or form tumors

211 When the agarose-GUVs are heated above the melting temperature of

212 ngs reveal potential artifactual behavior of agarose-GUVs in processes involving morphological change

213 racterize the mechanical properties of these agarose-GUVs in response to electric pulses, which induc

214 f poration, is significantly slowed down for agarose-GUVs when compared to agarose-free GUVs.

215 calcite crystals by a matrix composed of an agarose hydrogel on top of a carboxylate-terminated self

216 nsor Lumisens III using immobilized cells in agarose hydrogel, allowed to detect artificial mercury c

217 is formed between an aqueous droplet and an agarose hydrogel, which allows imaging in addition to el

218 mechanical properties of chondrocyte-seeded agarose hydrogels relative to unloaded free swelling con

219 on of the vesicles were found to encapsulate agarose in the form of a gel-like meshwork.

220 es-on native agarose and chemically modified agarose in the gel phase for the first time.

221 rmed using 7.5% (w/w) gelatin and 1.5% (w/w) agarose in the presence of variable amounts of polydextr

222 In our strategy, monodisperse 1.5 nL agarose-in-oil droplets are produced with a high frequen

223 Agarose-in-plug and capillary assays showed that these t

224 BGAF-Glu1 complex could still bind lactosyl-agarose, indicating that the sugar-binding site of BGAF

225 oes not require the sample to be embedded in agarose; instead, samples are prepared conventionally on

226 However, agarose is left encapsulated in the vesicles in differen

227 physically constrained by an inert material (agarose), is sufficient to induce formation of purse str

228 s of hydrogels derived from polysaccharides (agarose, kappa-carrageenan) having an alpha-helical back

229 as been sandwiched between two air-insulated agarose layers which gel in situ.

230 M gene reduced bacterial replication on 0.3% agarose low Mg(2+) media but not in low Mg(2+) liquid me

231 acillus thermocatenulatus lipase 2, BTL2) on agarose macroporous beads, followed by covalent coupling

232 CC formed more spheroids (orospheres) in 3-D agarose matrices or ultra-low attachment plates than con

233 The nuclei are then embedded in an agarose matrix containing numerous pores, allowing the a

234 Immobilization of a potent GSM onto an agarose matrix quantitatively recovered Pen-2 and to a l

235 a growth-restricting hydrogel composed of an agarose matrix with a second coating of agarose to form

236 aryotic cells, but because it is based on an agarose matrix, it is not always optimal for all protein

237 purified and subsequently manipulated in the agarose matrix.

238 now report that Salmonella can move on 0.3% agarose media in a flagella-independent manner when expe

239 ere found to readily germinate even on water agarose medium.

240 on mass spectrometric imaging (MALDI-MSI) of agarose micro-beads randomly arrayed at high-density in

241 Here, 3D-concave agarose micro-wells were used to culture robust pancreat

242 n aminoglycoside library immobilized onto an agarose microarray was probed for binding to a 3 x 3 nuc

243 Porous agarose microbeads, with high surface to volume ratios a

244 rmed using alpha-chymotrypsin immobilised on agarose microparticles.

245 uents of sweat) are collected into hydrogel (agarose) micropatches.

246 dissociated zebrafish retinal progenitors in agarose microwells.

247 HA mobility in 0.5% agarose minigels was found to be linearly related to the

248 ls how random, three-dimensional networks of agarose nanofibers are incorporated into single crystals

249 ydextrose prevents the formation of a stable agarose network, with the polysaccharide chains dispersi

250 carbon nanotubes (SWNTs) confined in porous agarose networks.

251 of agarose by S. degradans through cleaving agarose oligomers into neoagarobiose products that can b

252 is encased either in a rectangular block of agarose or between Formvar films suspended on a wire loo

253 nhibiting cell-free virus transmission using agarose or neutralizing antibodies, we show that EGCG in

254 munoprecipitated with the HC-HA complex from agarose-overlaid AM cell extracts by an anti-human Ialph

255 Using an agarose overlay to trap the HA-containing matrix, the HC

256 roblasts form the HC-HA-PTX3 complex with an agarose overlay.

257 tly pseudopods to blebs when migrating under agarose overlays of increasing stiffness.

258 IMER) is compared to that of a conventional, agarose packed bed, pepsin IMER column commonly used in

259 volves seeding and growing bacteria on small agarose pads and imaging the resulting microcolonies.

260 In this study, agarose particles were modified with MANAE, PEI and glyo

261 obilized on the surface of a 50 muM magnetic agarose particles, the limit of detection of rcHRPII was

262 he proposed technique was validated by using agarose phantoms.

263 rabidopsis thaliana) seedling roots grown on agarose plates.

264 ight (HMW) DNA, are isolated and embedded in agarose plugs.

265 In the presence of poration, agarose polymers prevent complete pore closure and lead

266 t inhibited when plasmids are immobilized in agarose prior to addition of egg extract.

267 tform, we demonstrate that soft-carboxylated agarose provides a unique environment for the polarizati

268 ootprinting, immunoprecipitation, and an ATP-agarose pull-down assay, EGCG was found to directly modu

269 denosine triphosphate-agarose and calmodulin-agarose pull-down assays show that the TRPV6-ARD does no

270 Microcystin-agarose pull-downs suggested that a phosphatase binds to

271 SA-agarose pulldown and immunoblotting for IRF5 were used t

272 vacuolar hemin exporter, results with hemin-agarose pulldown assays showed that Abc3 binds to hemin.

273 nalysis by absorbance spectroscopy and hemin-agarose pulldown assays showed that Shu1 interacts with

274 T1A into giant unilamellar vesicles using an agarose rehydration method.

275 of S. marcescens secretomes with polymyxin B agarose rendered secretomes unable to inhibit epithelial

276 n their P3 coat protein were bound to nickel agarose resin and were subsequently challenged with a pr

277 ancer cells (OEC-M1) were encapsulated in 3D agarose scaffold and cultured in a miniaturized chamber

278 Agarose sensors showed a 40 nm wavelength shift from 0 t

279 Comparison of cells in 1% and 3% agarose showed that cells in the stiffer gels rapidly de

280 Affinity assays using cGMP-immobilized agarose showed that only activated PKGIalpha binds RhoA,

281 The melted agarose solution containing a redox dye tetranitroblue t

282 urthermore, we show that migration under the agarose spot can be modulated by selective small molecul

283 against this gradient by crawling under the agarose spots towards their centre.

284 okine gradients by a simple stamping method: agarose stamps were soaked with chemokine solution to fo

285 s in monolayer culture and three-dimensional agarose, suggesting a role for cell adhesion.

286 e integrative repair by GFP+ cells seeded in agarose, supporting their potential use in cartilage the

287 Derivatized-agarose supports are suitable for enzyme immobilization

288 We showed that on the agarose surface under physiological conditions, E-cadher

289 f an agarose matrix with a second coating of agarose to form 6- to 8-mm diameter macrobeads.

290 -based systems, the suitability of different agarose types, agarose concentrations, and buffer system

291 rase AgaD requires at least four consecutive agarose units (DP8) and is highly intolerant to modifica

292 ably softer hydrogels made from carboxylated agarose use a scaffold of unpaired beta-strands.

293 The bacteria that metabolize agarose use multiple enzymes of complementary specificit

294 We show that CbpA binds to cAMP-conjugated agarose via its C-terminal CAP domain.

295 roitin sulfate) that was covalently bound to agarose via terminal amine groups, and the variables exa

296 response time of immobilized T. thioparus on agarose was also found equal to 120 s at agarose concent

297 ctyostelium cells were observed moving under agarose, which efficiently induces blebbing, and the dyn

298 helix in the highly rigid hydrogel of native agarose, while the considerably softer hydrogels made fr

299 circuit boards, made with centimeter-length agarose wires.

300 We encapsulated bacteria in agarose with a user-defined stiffness, measured the grow