ライフサイエンスコーパス: agarose gel

1 .22 in solution to 1.2 +/- 0.04 in a 3 w/v % agarose gel.

2 bilayer is sandwiched between two layers of agarose gel.

3 native conditions through polyacrylamide or agarose gel.

4 slationally immobile in a low weight percent agarose gel.

5 double-stranded DNA that was detected on an agarose gel.

6 resembles a simple salt solution as in a 4% agarose gel.

7 ia diffusion into the network that forms the agarose gel.

8 Expulsion occurs in an agarose gel.

9 e and subjected to electrophoresis on a 1.5% agarose gel.

10 the density of MIMIC to target cDNA bands on agarose gel.

11 lectrophoresis of digestion products in 1.5% agarose gel.

12 idyl-prolyl isomerase-were immobilized on an agarose gel.

13 s of iron(III) immobilized on iminodiacetate-agarose gel.

14 ere identified after electrophoresis in 1.5% agarose gel.

15 s as easy, convenient, and inexpensive as an agarose gel.

16 digestion, and detection of fragments on an agarose gel.

17 ass substrate while they are diffusing in an agarose gel.

18 rcoiled (sc) form of plasmid DNA (pDNA) from agarose gel.

19 ering was evaluated by electrophoresis on 3% agarose gel.

20 lso used to extract the sc form of pDNA from agarose gel.

21 as quantitated by radial diffusion in fibrin-agarose gel.

22 Titin isoform expression was evaluated with agarose gels.

23 restriction of translational mobility in 1% agarose gels.

24 to resemble spherical obstacles embedded in agarose gels.

25 esis in 20% polyacrylamide-8 M urea gels and agarose gels.

26 PCR products were detected in agarose gels.

27 per band in post-electrophoretically stained agarose gels.

28 formation of supershifted species on native agarose gels.

29 nds, having slightly different mobilities in agarose gels.

30 und to be applicable with 0.8, 1.0, and 2.0% agarose gels.

31 ansformants had an aberrant mobility through agarose gels.

32 ysis of restriction digests on nondenaturing agarose gels.

33 de fluorescence of PCR products excised from agarose gels.

34 hich uses SYBR Green I to stain DNA in dried agarose gels.

35 gested with MspI and were electrophoresed on agarose gels.

36 tes yielded similar results when analyzed on agarose gels.

37 ed only simple Y patterns in two-dimensional agarose gels.

38 ctrophoretic transport of lambda-DNA through agarose gels.

39 DNA that can be visualized in vivo using 2D agarose gels.

40 etected as the corresponding PCR amplicon in agarose gels.

41 ecular mass as low as approximately 9 kDa in agarose gels.

42 breast cancer cells seeded into nonadhesive agarose gels.

43 ion in the Darcy permeability of 3 vol/vol % agarose gels.

44 rystals can be achieved by crystal growth in agarose gel, a naturally occurring chiral polysaccharide

45 els, reconstituted basement membrane matrix, agarose gels, alginate gels, and fibrin gels, but not in

46 replication models based on two-dimensional agarose gel analyses.

47 The conditions used for 2D agarose gel analysis are favorable for branch migration

48 was assessed by Western blotting and native agarose gel analysis in Huh7 cells, and the human immune

49 have been experimentally validated by QPCR, agarose gel analysis, sequencing and BLAST, and all vali

50 fined plasmid substrates and two-dimensional agarose gel analysis, we examined the collision of an ac

51 rk integrity as monitored by two-dimensional agarose gel analysis.

52 205 squamous carcinoma cells embedded in an agarose gel and cell spheroids in Matrigel.

53 determined from 32 phantoms constructed with agarose gel and in eight concentrations from each of the

54 maging studies, in infusion experiments with agarose gel and in vivo rat brain studies simulating cli

55 Ligation was verified by agarose gel and small-angle X-ray scattering.

56 The same irradiated samples were analyzed by agarose gel and SSB yields were determined by convention

57 es derived from densitometric scanning of an agarose gel and those derived from the SCFluo method wer

58 tilting angle of the microtubules buried in agarose gel and to find the precise surface plasmon reso

59 adily assayed by electrophoresis on standard agarose gels and because a public database of over 25,00

60 ing the uptake of (45)Ca by isolated ACVs in agarose gels and by ACVs in situ in freeze-thawed cartil

61 ells were identified by DNA fragmentation in agarose gels and by Hoechst staining.

62 migrated with undigested parental capsids on agarose gels and cosedimented with undigested capsids by

63 results in enhanced 3 dimensional growth in agarose gels and in long-term cultures within matrigel.

64 nated the need to load, run, stain, and read agarose gels and provided the advantage of instant detec

65 fI, followed by submarine electrophoresis in agarose gels and staining with ethidium bromide, produce

66 ladder-like fragmentation of genomic DNA in agarose gels and the intense blue fluorescence exhibited

67 on to all AP-PCR profiles was extracted from agarose gels and then cloned and sequenced.

68 DNA laddering (agarose gels) and terminal deoxynucleotidyl transferase-

69 13 were positive only by the assay with the agarose gel, and 3 were positive only by the assay with

70 g protein calmodulin (CaM) immobilized in an agarose gel, and we have demonstrated the application of

71 d (female) were isolated, electrophoresed in agarose gels, and transferred to nylon membranes.

72 or 12 h, separated by electrophoresis on 2 % agarose gels, and visualized with ethidium bromide stain

73 Gels are run horizontally in a standard mini-agarose gel apparatus.

74 Agarose gels are viscoelastic soft solids that display a

75 10-, 20-, and 30-mmol/L NaCl solution and 5% agarose gel as a reference.

76 Using carboxylated agarose gels as a screening platform, we demonstrate tha

77 ligonucleosomal fragmentation was visible on agarose gels as early as 60 or 30 min after PDT, respect

78 Native agarose gel assays corroborated this finding.

79 in protocol A by increasing the sigma on the agarose gel at a constant rate to define the cardiocyte

80 ision and integration, we first developed an agarose gel-based assay for CTnDOT recombination, which

81 PCR with urine combined with agarose gel-based detection was 66.9% sensitive and 98.3

82 ene, designated UROC28, was identified by an agarose gel-based differential display technique, and it

83 sis-based five-enzyme (SNaPshot) method, the agarose gel-based one-enzyme method, and the automatic s

84 is of 0.1% (w/v) 90% esterified pectin in an agarose gel by diffused, commercial PME were log-linear

85 n be directly visualized after separation in agarose gels by ethidium bromide staining.

86 monella specific on ethidium bromide-stained agarose gels by Southern hybridization with a 20-mer oli

87 The pore size, a, of a 2% agarose gel cast in a 0.1 M PBS solution was estimated.

88 Using two-dimensional agarose gels, chemical probing and atomic force microsco

89 MVm and C5a) compared with WT using an under-agarose gel chemotaxis assay.

90 Selected PCR products were eluted from agarose gels, cloned, and sequenced.

91 nded DNA fragments with various DNA markers, agarose gel concentrations, and field strengths.

92 DNA agarose gels confirmed morphological identification of a

93 In this protocol, droplets of agarose gel containing different chemokines are applied

94 s ever observed for compression of uncharged agarose gel controls.

95 trap the synaptic complex observed on native agarose gels correlated with its potency for inhibiting

96 present work proposes the improvement of an agarose gel DNA electrophoresis in order to allow for a

97 the presence of nucleosomal DNA fragments on agarose gels (DNA ladder) and in situ nick end labeling.

98 describe the development of 2D intact mtDNA agarose gel electrophoresis (2D-IMAGE) for the separatio

99 eosomal arrays were determined by analytical agarose gel electrophoresis (AAGE) and single molecules

100 HPLC analysis was validated in parallel with agarose gel electrophoresis (AGE), enzyme digestion, and

101 rformance liquid chromatography (AEC) and an agarose gel electrophoresis (AGE)-based method developed

102 in BPAEC treated with 2-ME was identified by agarose gel electrophoresis (DNA ladder) as well as in s

103 d in vitro using a low-temperature EDTA-free agarose gel electrophoresis (LTEAGE) procedure.

104 luated PCR product detection by using either agarose gel electrophoresis (PCR-gel) or dot blot hybrid

105 d tissue was measured using a combination of agarose gel electrophoresis and a radiometric assay.

106 block architectures were characterized by 1% agarose gel electrophoresis and atomic force microscope

107 d tubules and 20 glomeruli were separated by agarose gel electrophoresis and by isoelectric focusing,

108 Apoptosis was determined using agarose gel electrophoresis and by measuring the cytopla

109 We used two-dimensional agarose gel electrophoresis and electron microscopy to a

110 With alkaline agarose gel electrophoresis and filter blot hybridizatio

111 NE increased DNA laddering on agarose gel electrophoresis and increased the percentage

112 ling, aggregation, loss of resolution during agarose gel electrophoresis and loss of transformation a

113 n comparing MIRU-VNTR profiles obtained from agarose gel electrophoresis and PCRs analyzed on a WAVE

114 The quantity and quality were confirmed by agarose gel electrophoresis and polymerase chain reactio

115 DNA fragmentation determined by agarose gel electrophoresis and quantitation of [3H]thym

116 y a dye-doped silica shell were separated by agarose gel electrophoresis and scanned by a conventiona

117 urse of infection by one and two-dimensional agarose gel electrophoresis and Southern hybridization.

118 t method, the DNA segments were separated by agarose gel electrophoresis and stained with ethidium br

119 ucts (e.g., open circle and linear forms) by agarose gel electrophoresis and subsequently quantified

120 csL was also examined at pH 8.0 by using SDS-agarose gel electrophoresis and transmission electron mi

121 Agarose gel electrophoresis and ultraviolet spectrophoto

122 reaction (PCR) are exploited using on-column agarose gel electrophoresis as separation and inductivel

123 By adopting a native agarose gel electrophoresis assay that can specifically

124 Native agarose gel electrophoresis confirmed that FliH and FliI

125 ation of density gradient centrifugation and agarose gel electrophoresis coupled with probes specific

126 A direct comparison of SSDS with agarose gel electrophoresis for +/- screening shows that

127 capsids that migrated more slowly in native agarose gel electrophoresis from A36V mutant than from t

128 e liver using atomic force microscopy and 2D agarose gel electrophoresis in order to resolve this iss

129 the SDS-treated full-length particles during agarose gel electrophoresis is most likely caused by dis

130 In the current work, an improved agarose gel electrophoresis method for analysis of high

131 The widely used agarose gel electrophoresis method for assessing radiati

132 However, here, non-denaturing agarose gel electrophoresis of bacteriophage T7 reveals

133 Agarose gel electrophoresis of DNA and RNA is routinely

134 over, DNA laddering was shown in myocytes by agarose gel electrophoresis of DNA fragments.

135 their retinas were evaluated by morphology, agarose gel electrophoresis of DNA, in situ terminal deo

136 onucleosomal DNA degradation was assessed by agarose gel electrophoresis of DNA, which showed DNA fra

137 inhibitor, lovastatin, and was evaluated by agarose gel electrophoresis of genomic DNA, morphologica

138 d using classical morphological features and agarose gel electrophoresis of genomic DNA.

139 minimize errors and is broadly applicable to agarose gel electrophoresis of RNA samples and their sub

140 loci was determined by PCR amplification and agarose gel electrophoresis of the amplicons.

141 articles, were stable during purification by agarose gel electrophoresis or sucrose density gradient

142 An agarose gel electrophoresis pre-screening strategy ident

143 cles successfully separated by using a novel agarose gel electrophoresis procedure.

144 Agarose gel electrophoresis provided further biochemical

145 -screening non-sequence verified clones with agarose gel electrophoresis provides an inexpensive and

146 In addition, agarose gel electrophoresis revealed a 49% increase (P <

147 ild-type and mutant plants by single-nucleus agarose gel electrophoresis revealed that bleomycin-indu

148 Agarose gel electrophoresis revealed that KA (2.5 nmol)

149 Two-dimensional agarose gel electrophoresis revealed that ORC2 depletion

150 alysis of mammalian mtDNA by two-dimensional agarose gel electrophoresis revealed two classes of repl

151 We verified these AFM results by agarose gel electrophoresis separation of UV-irradiated

152 Agarose gel electrophoresis showed that high molecular w

153 d sequences were used in conjunction with an agarose gel electrophoresis system incorporating an AT-b

154 We show by two-dimensional (2D) agarose gel electrophoresis that replication forks natur

155 degrees C and 37 degrees C and shown by SDS-agarose gel electrophoresis to be comprised of a large,

156 mplified, and the amplicons were analyzed by agarose gel electrophoresis to determine the copy number

157 We used plasmid pBR322 and two-dimensional agarose gel electrophoresis to examine the collision of

158 genetics and high resolution two-dimensional agarose gel electrophoresis to examine the torsional ten

159 We apply agarose gel electrophoresis to sensitively evaluate prot

160 ation forks recover, we used two-dimensional agarose gel electrophoresis to show that replication-blo

161 Agarose gel electrophoresis was subsequently used to sep

162 Using two-dimensional agarose gel electrophoresis we also show that UvsW acts

163 om the present method and those derived from agarose gel electrophoresis were compared.

164 was more sensitive than conventional PCR and agarose gel electrophoresis with ultraviolet transillumi

165 cts from all of the reactions, visualized by agarose gel electrophoresis, allowed immediate identific

166 ts of 0.95, 1.3, and 1.8 kb were detected by agarose gel electrophoresis, although the transcripts hy

167 ed cells, using two-dimensional (2D) neutral agarose gel electrophoresis, and in a cell-free SV40 DNA

168 PCR amplification, agarose gel electrophoresis, and sequencing methods were

169 ed with DNA fragmentation when determined by agarose gel electrophoresis, as seen in the case of THP-

170 ic internucleosomal ladder of genomic DNA by agarose gel electrophoresis, by finding nuclear fragment

171 Agarose gel electrophoresis, circular dichroism and diff

172 analytical ultracentrifugation, quantitative agarose gel electrophoresis, electron cryomicroscopy, an

173 are based on two-dimensional, non-denaturing agarose gel electrophoresis, followed by structure deter

174 chromatin condensation, DNA fragmentation by agarose gel electrophoresis, or terminal deoxynucleotidy

175 nnector or the procapsid, as investigated by agarose gel electrophoresis, SDS-PAGE, sucrose gradient

176 nation of TUNEL staining and pulse-field and agarose gel electrophoresis, suggesting a predominantly

177 ple criteria, including DNA fragmentation by agarose gel electrophoresis, terminal deoxynucleotidyltr

178 The amplified DNA was separated by agarose gel electrophoresis, transferred to nylon membra

179 Here, by native agarose gel electrophoresis, using recombinant IN with a

180 ragment length polymorphism (RFLP) typing by agarose gel electrophoresis, we compared the analyzer wi

181 Using two-dimensional agarose gel electrophoresis, we examined DNA replication

182 Using two-dimensional agarose gel electrophoresis, we show that mitochondrial

183 Using one- and two-dimensional agarose gel electrophoresis, we show that the linear mtD

184 Using 2D agarose gel electrophoresis, we show that the six o'cloc

185 ported by analysis of mtDNA molecules by 2-D agarose gel electrophoresis, which indicated the presenc

186 blot and DNA fragmentation was determined by agarose gel electrophoresis.

187 ry, terminal dUTP nick-end labeling, and DNA agarose gel electrophoresis.

188 SA and apo(a) size by Western blot after SDS-agarose gel electrophoresis.

189 light of new data using two-dimensional (2D) agarose gel electrophoresis.

190 rphisms were detected at 9 of the 11 loci by agarose gel electrophoresis.

191 ells was analyzed by native, two-dimensional agarose gel electrophoresis.

192 om the amplification product of viral RNA by agarose gel electrophoresis.

193 nd the restriction fragments are resolved by agarose gel electrophoresis.

194 cies-specific fingerprints for comparison by agarose gel electrophoresis.

195 SA and apo(a) size by Western blot after SDS-agarose gel electrophoresis.

196 hese DNA fragments are typically analyzed by agarose gel electrophoresis.

197 leosomal DNA degradation was assessed by DNA agarose gel electrophoresis.

198 osomal fragmentation (ladder pattern) by DNA agarose gel electrophoresis.

199 quirement of 2 days to examine 96 samples by agarose gel electrophoresis.

200 size distributions using denaturing glyoxal-agarose gel electrophoresis.

201 dUTP terminal nick-end labeling assay and by agarose gel electrophoresis.

202 nt length polymorphisms (RFLPs) generated by agarose gel electrophoresis.

203 nick end labeling (TUNEL) histochemistry and agarose gel electrophoresis.

204 as associated with DNA laddering as shown by agarose gel electrophoresis.

205 mplicons with sizes easily differentiated by agarose gel electrophoresis.

206 onfirmed by restriction enzyme digestion and agarose gel electrophoresis.

207 viability and the fragmentation of DNA using agarose gel electrophoresis.

208 ved with HindIII and HaeIII and subjected to agarose gel electrophoresis.

209 idence of DNA fragmentation when analyzed by agarose gel electrophoresis.

210 opoisomerase I (Topo I), and two-dimensional agarose gel electrophoresis.

211 ation was investigated in tissue extracts by agarose gel electrophoresis.

212 enzymes HhaI, MboI, and AluI and analyzed by agarose gel electrophoresis.

213 gher sensitivity as compared to conventional agarose gel electrophoresis.

214 termediates were examined by two-dimensional agarose gel electrophoresis.

215 ng transmission electron microscopy and 1.2% agarose gel electrophoresis.

216 ugation, and retarded mobility during native agarose gel electrophoresis.

217 wed by amplifications with ISSR and SCoT and agarose gel electrophoresis.

218 when positive, and results were verified by agarose gel electrophoresis.

219 ed, as revealed by semi-denaturing detergent agarose gel electrophoresis.

220 d transfer complex can be isolated by native agarose gel electrophoresis.

221 generate monovalent quantum dots (QDs) using agarose gel electrophoresis.

222 used to detect apoptosis, including: a) DNA agarose gel electrophoresis; b) terminal deoxynucleotidy

223 purifying DNA-origami nanostructures rely on agarose-gel electrophoresis (AGE) for separation.

224 d-UTP nick-end labeling (TUNEL) staining and agarose-gel electrophoresis of extracted slice DNA.

225 Agarose-gel electrophoresis was performed on all serum s

226 Using two-dimensional agarose-gel electrophoresis, we show that there is no sp

227 rmed by "deoxyribonucleic acid laddering" on agarose-gel electrophoresis.

228 e-specific PCR qualitative assay followed by agarose-gel electrophoresis.

229 Based on a combination of two-dimensional agarose gel electrophoretic analysis and mapping of 5' e

230 Arabidopsis roots grown on inclined agarose gels exhibit a sinusoidal growth pattern known a

231 medium model permitting the estimation of an agarose gel fiber radius and hydraulic permeability of t

232 ries of twofold dilutions of total DNA in an agarose gel followed by ethidium bromide staining, and s

233 rpo105-rpo273), followed by analysis on a 4% agarose gel for a 168-bp product.

234 lysis of the UsCPV genome segments (using 1% agarose gels) generated a migration pattern (electropher

235 Selective adsorption onto agarose gels has become a powerful method to separate si

236 its immobilization in low-weight-percentage agarose gels; however, fusion of CaM to MBP via a flexib

237 ng software package to automatically analyze agarose gel images of polymorphic DNA markers.

238 by bovine pancreatic trypsin immobilised on agarose gel in 100 mM ammonium hydrocarbonate buffer, pH

239 sing an electrophoretic separation on native agarose gels in combination with polymerase chain reacti

240 documented by DNA nick-end labeling, or DNA agarose gels in xenografts of human hematopoietic tumors

241 Aqueous agarose gels infused with 48 wt % NaClO3 at 6 degrees C,

242 In studies of in vitro agarose gel infusion, the use of functionalized Gd(3)N@C

243 rget binding functionality of CaM assayed in agarose gels is in good agreement with solution assays.

244 ing kinetics and compare the drying speed of agarose gels loaded with various non-gelling saccharides

245 the mobility of natural linear HA chains on agarose gels, making the complexes useful as defined siz

246 Immobilization in an agarose gel matrix eliminates potential interactions of

247 The YAC is maintained in an agarose gel matrix to avoid damage until the final steps

248 and only one-third the time compared to the agarose gel method.

249 orescence analysis of treponemes embedded in agarose gel microdroplets revealed that only minor porti

250 ings imply that the SSB yields inferred from agarose gels need reevaluation, especially when they wer

251 on fragment cloned from a band visible in an agarose gel of Pinus lambertiana (sugar pine) genomic DN

252 tall the extension of the acrosome bundle in agarose gels of different concentrations.

253 The reptation in agarose gels of H3/H4 tetramer arrays is essentially ind

254 tudy by in-situ interferometry the drying of agarose gels of various compositions cast in Petri dishe

255 %) and without contaminants such as residual agarose gel or DNA intercalating dyes.

256 these cultivars were killed by excess Al in agarose gels or in simple salt solutions.

257 sed interaction of proteoglycan with HCII in agarose gels paralleled increased activity in thrombin-H

258 oefficients for acetylcholine and choline in agarose gel perfused with physiological solutions were d

259 The computational results are validated with agarose gel phantom experiments.

260 n were preloaded with fluo-4, cast into a 1% agarose gel, placed above the compound sheets, and image

261 ssure-hypertrophied cats were embedded in an agarose gel, placed on a stretching device, and subjecte

262 purchased contained the desired cDNA clone, agarose gel pre-screening, colony isolation and similari

263 mical laser-induced nucleation of an aqueous agarose gel prepared with supersaturated potassium chlor

264 e restriction enzyme, HinfI, was run on 0.7% agarose gels, probed with radiolabeled (AATCCC)4, and ex

265 ted by the electrophoresis in polyacrylamide/agarose gel profile.

266 s measured, and the results suggest that the agarose gel reduces the effective supersaturation of the

267 Analysis of RIs on two-dimensional agarose gels revealed a rapid loss in the EBV bubble arc

268 SDS-agarose gels revealed small N2B (stiff) and large N2BA (

269 ent pH changes throughout a nanowire network/agarose gel sample during external solution pH changes,

270 ons were produced following infusions in six agarose gel samples at 2.4 T and from direct brain infus

271 ere analyzed by electrophoresis performed on agarose gel; samples with a discrete or localized band w

272 lly occurring X and M13 ssDNAs (as judged by agarose gel-shift assays and electron microscopic analys

273 A simple method for extracting DNA from agarose gel slices is described.

274 were separated by electrophoresis on a 0.8% agarose gel stained with ethidium bromide.

275 forms were produced and observed directly in agarose gels stained with Vistra Green and imaged with a

276 n these experiments, mice were given vaginal agarose gel suppositories containing either 5 mg OVA or

277 lts demonstrate that any modification to the agarose gel surface and, consequently, the permanent dip

278 A native agarose gel system was used to evaluate telomere DNA-bin

279 cted a new nucleoprotein complex on a native agarose gel that was produced in the presence of >200 nM

280 aging of arrays of qdots localized in dilute agarose gel, the blinking of qdots was measured across f

281 On agarose gels, the buffers in various concentrations were

282 al diameter, 0.579 mm) shallowly embedded in agarose gel, then to a collection reservoir.

283 Due to their migration in agarose gels, these incomplete physical forms of DNA hav

284 that saccharides systematically decrease the agarose gel thinning rate up to a factor two, and exempl

285 Isolated hepatocytes were cast in agarose gel threads and perfused with Krebs-Henseleit bi

286 obleaching (FRAP) both in solution and in 2% agarose gels to compare transport properties of these ma

287 fragments were size separated on analytical agarose gels to create DNA fingerprints.

288 technique employs topographically patterned agarose gels to deliver various membrane preparations to

289 sensors in conjunction with collagen-coupled agarose gels to detect subcellular activities of SFK and

290 ection of 10 muL cell inclusions in cm-sized agarose gels used here as phantom models of microtumors.

291 nanes that migrate in different bands on the agarose gels used to analyse the products of the reactio

292 n situ for 96 h to 200 microM total Al in an agarose gel was significantly less than that of cv Dade

293 To probe that question, U(IV) immobilized in agarose gels was exposed to conditions allowing biologic

294 Heterogeneity within agarose gels was modeled by assuming that fiber-rich, sp

295 domly distributed fluorescent nanospheres in agarose gel were obtained and fitted with the theoretica

296 electrophoresis and zone electrophoresis on agarose gel were used to monitor reaction conditions for

297 ctional blots to evaluate band modulation on agarose gels which are initially run to evaluate the rea

298 We use micropatterned, enzyme-laden agarose gels which are stamped on polyacrylamide films c

299 125I-LDL bound to HL-saturated heparin-agarose gel with a Kd of 52 nM, and somewhat surprisingl

300 ns < or = 0.011) were covalently attached to agarose gels with volume fractions of 0.040 or 0.080.