1 Compared to
an ail mutant, additional deletion of psaA (encoding Psa) l
2 phenotype, repressing the expression of
both ail transcript levels and cell surface-expressed Ail pro
3 However, a KIM5
Delta ail mutant had decreased binding to host cells, while a
4 berculosis homologs of the Y.
enterocolitica ail and the Y. pestis psa loci were identified.
5 group of mini-Tn10 mutants, which
expressed ail in stationary phase at 28 degrees C.
6 indicated that only mutations in psa, not
in ail, resulted in defects for thermoinducible binding, wi
7 pentapeptide instead of the QACRG present
in ail known ASCPs.
8 that is highly correlated with virulence
is ail, a gene identified along with inv in a screen for Y.
9 rmal rat serum, but not mouse serum, to
kill ail-negative Y. pestis in vitro.
10 lasmid pYV and the attachment invasion
locus ail.
11 Analysis
of ail transcripts in the wild-type and clpP mutant strains
12 Expression
of ail in stationary phase at 28 degrees C was also derepre
13 Inactivation
of ail resulted in loss of fH binding.
14 The expression level
of ail was also the highest of the three adhesins in infect
15 Thus, ClpP has a role in the modulation
of ail transcription in Y. enterocolitica.
16 letion and point mutations in the regions
of ail predicted to encode the cell surface loops.
17 e is a single start site of transcription
of ail and that the effect of the clpP mutation was on the
18 A Y.
pestis ail deletion mutant was rapidly killed by sera obtained
19 The Y.
pestis ail mutant was attenuated for virulence in both rodent m
20 In stationary
phase,
ail transcript is only detected when bacteria are grown
21 stA, responsible for enterotoxin
production,
ail, attachment invasion locus gene, and rfbC, O-antigen
22 Mutations in the Y.
pseudotuberculosis ail and psa loci were constructed and tested for thermoi
23 The ail gene of Y. enterocolitica is regulated by temperatur
24 Although
the ail mutant showed only a modest decrease in cell binding
25 Here, we deleted
the ail gene (encoding the attachment-invasion locus) from w
26 tructed mutant with a single deletion in
the ail locus (y1324) was severely defective for delivery of
27 Together, deletion of
the ail gene from the Deltalpp DeltamsbB double mutant sever
28 Disruption of
the ail gene in Y. pseudotuberculosis resulted in loss of C4
29 Intradermal infection with
the ail mutant resulted in an atypical, subacute form of bub
30 that emerged contained insertions within
the ail (attachment and invasion locus) gene of Y. pestis.
31 ne shares limited homology with the
Yersinia ail locus and significant homology with the hra1 aggluti