ライフサイエンスコーパス: alanine residue

1 teine residue on human TS to a corresponding alanine residue.

2 he KMSKS motif is replaced by a serine or an alanine residue.

3 cysteine residue, C477 was replaced with an alanine residue.

4 d the amino acid terminus was extended by an alanine residue.

5 eased when this proline was replaced with an alanine residue.

6 e, Asp121 was replaced with an asparagine or alanine residue.

7 hed by conversion of Cys481 to a nonreactive alanine residue.

8 e of the active site has been replaced by an alanine residue.

9 e in the position adjacent to the N-terminal alanine residue.

10 ctive site nucleophile in this family, to an alanine residue.

11 onversion of mutated Cys(37) into the native alanine residue.

12 nded to the 13(1)-keto group of P(A) with an alanine residue.

13 sters of charged amino acids were changed to alanine residues.

14 ent on the proximity and the location of the alanine residues.

15 ated glycine-based peptides containing three alanine residues.

16 nd 719, with either aspartate, glutamine, or alanine residues.

17 phosphorylated during mitosis substituted by alanine residues.

18 of PrP in which cysteines were replaced with alanine residues.

19 ed six BPTI mutants containing from 21 to 29 alanine residues.

20 h contains proline residues in place of some alanine residues.

21 ding the consensus sequence were replaced by alanine residues.

22 ered by exchanging two arginine residues for alanine residues.

23 ine is replaced independently with a pair of alanine residues.

24 of the protein, a region rich in proline and alanine residues.

25 all three cysteine residues were replaced by alanine residues.

26 in the zinc finger motif were replaced with alanine residues.

27 s7 and Arg10 (P2 subsite) were replaced with alanine residues.

28 ng the codons for bilin-binding cysteines to alanine residues.

29 mAChR with residues Thr307-Ser311 mutated to alanine residues.

30 ny other C-terminal loop, were replaced with alanine residues.

31 H2, three leucines are substituted for three alanine residues.

32 te resistance pump was changed to serine and alanine residues.

33 amino acid phosphinothricin attached to two alanine residues.

34 tin-3, selectively labeled with (15)N at all alanine residues.

35 eu28, Pro29, and Ile34 were substituted with alanine residues.

36 h of the three sites were mutated to neutral alanine residues.

37 casein kinase 1 (CK1) sites are replaced by alanine residues.

38 ino acids of the Tra domain were replaced by alanine residues.

39 quired but could be conferred by a series of alanine residues.

40 His-186 and Asn-189 have been exchanged for alanine residues.

41 esidues were replaced by nonphosphorylatable alanine residues.

42 90)RLSIGE(396)) was converted to a series of alanine residues.

43 utation of integral amino acid residues with alanine residues.

44 Pbeta in vivo and that a Z mutant altered at alanine residue 204 in the bZIP domain is impaired for t

45 ers the third position of a codon specifying alanine residue 293, without changing the predicted amin

46 Mutation of alanine residue 315 within the TM3 to tryptophan increas

47 onal NH...S(Cys) hydrogen bond involving the alanine residue 44.

48 g temperature of mutants containing up to 27 alanine residues (48 % of the total number of residues)

49 When the alanine residue (A6) in the atypical Walker-like A box o

50 antidotes or replacement of Cys-179 with an alanine residue abolished dye binding to and arsenite in

51 Substitution of the RHRRR sequence with alanine residues abolished raft localization of the CD4

52 predicted DXD glycosyltransferase motif with alanine residues abolished UDP-GlcNAc binding and lympho

53 putative catalytic carboxylate Asp44 with an alanine residue abolishes activity.

54 Thus, the alanine residue Ala(254) determines voltage-dependent re

55 bending the pore helix at a highly conserved alanine residue (Ala-621) below the gate is responsible

56 t to hydroxyapatite was investigated for two alanine residues (Ala(46) and Ala(49)) using (13)C{(31)P

57 e FVO strain Plasmodium falciparum AMA1 with alanine residues (ALA).

58 glycine residues for the corresponding PDE5 alanine residues, Ala(608) and Ala(612).

59 ever, mutagenesis of Asp-854 to a permissive alanine residue allows phosphorylation of Ser-853 on mGl

60 on retaining a high level of ester-linked D-alanine residues along the polyglycerol phosphate backbo

61 Addition or deletion of an alanine residue also causes a pronounced decrease in eff

62 d mutagenesis, we have changed His-252 to an alanine residue and analyzed the effect of this mutation

63 ble glutamate with either an aspartate or an alanine residue and determined the impact of the point m

64 ol positions were individually changed to an alanine residue and the binding affinities of the mutant

65 d upon mutation of either R571 or D753 to an alanine residue and thus these amino acids do not appear

66 e nascent peptide in the ribosome carries an alanine residue and, to a lesser extent, serine or threo

67 the ECD of a synthetic peptide containing 10 alanine residues and 6 lysine residues uniformly distrib

68 troanilide substrates most effectively after alanine residues and also displayed aminopeptidase activ

69 are small basic proteins rich in lysine and alanine residues and contain 9-amino-acid cleavable pres

70 harged amino acids in one or both loops with alanine residues and found that the positive charges are

71 residues at the C-terminal end of MCP-1 with alanine residues and tested these mutants for their abil

72 s containing single-cysteine replacements by alanine residues and the above three RP mutants have bee

73 mutagenesis, including changes of charged to alanine residues and uncharged to charged residues.

74 lethal mutations, and the presence of nearby alanines residues and nonlethal mutations.

75 ifferent cysteine residue was replaced by an alanine residue, and one luciferase mutant in which all

76 and Lys-104 were replaced individually by an alanine residue, and the resulting enzymes were assayed

77 lly mutagenized to create uncharged pairs of alanine residues, and 32 recombinant mutant viruses were

78 and 683 were replaced by nonphosphorylatable alanine residues, and a 5D mutant carrying aspartic acid

79 s at positions 658 or 679 were mutated to di-alanine residues, and the mutant receptors were stably e

80 nal peptide sequences with cleavage sites at alanine residues, and transmembrane domains on the C-ter

81 emains essentially unchanged when up to four alanine residues are changed to valine.

82 In the model of TL, four alanine residues are positioned in the binding site as c

83 ent to specify the fold of an LA module when alanine residues are present at all other positions.

84 n analogue peptides in which combinations of alanine residues are substituted for prolines.

85 this paper, the effect of the penultimate D-alanine residue (as opposed to a glycine residue) has be

86 creased affinity for ATP; 2) mutation of the alanine residue at 1331 to cysteine (A1331C) in the Walk

87 titution of an aspartic acid for a conserved alanine residue at amino acid 2655 (A2655D).

88 This mis-sense substitution results in an alanine residue at codon 49 being replaced with threonin

89 A Z mutant containing an alanine residue at position 186 [Z(S186A)] was significa

90 It is the substitution of the alanine residue at position 339 with proline that is pri

91 ty, indicating that interactions between the alanine residue at position 35 and these C-terminal regi

92 In this fashion, it was determined that the alanine residue at position 49 in FR-alpha was critical

93 the six acidic residues in the context of an alanine residue at position 98 (Y98A) relative to an aro

94 A conjugate containing a beta-alanine residue at the C terminus of the polyamide moiet

95 Insertion of an alanine residue at the N-terminus of the mature vWF subu

96 Furthermore, a glutamate at +2 and two alanine residues at -3 and -1 to the second cleavage sit

97 LA variants with alanine residues at as many as 18 of 37 positions fold t

98 cal LA module by constructing sequences with alanine residues at nonconserved positions in the module

99 l and calcium were decreased by insertion of alanine residues at position 51 of the mature protein an

100 Alanine residues at position 98 (located near the [4Fe-4

101 When the three alanine residues at positions -3 to -1 in the native sig

102 t, recombinant VZVs that expressed IE62 with alanine residues at S686, the suspected target by which

103 omewhat longer helices may be made by adding alanine residues at the C terminus.

104 iparallel tetramers shows that the burial of alanine residues at the e positions between the neighbor

105 epared dual-cavity basket 1 to carry six (S)-alanine residues at the entrance of its two juxtaposed c

106 e protein (Ala-14) that contains exclusively alanine residues at the hydrophobic a and d positions of

107 s were performed in this study: insertion of alanine residues between the cysteine residues of the Cy

108 ion within the seco-cryptophycin unit C beta-alanine residue, but strict structural requirements at t

109 466 of choline oxidase was replaced with an alanine residue by site-directed mutagenesis and the bio

110 28, were each independently replaced with an alanine residue by site-directed mutagenesis.

111 bstitution of the RRKR motif of MT-MMP1 with alanine residues by site-directed mutagenesis resulted i

112 e phosphatase sequence motif were changed to alanine residues by site-directed mutagenesis.

113 ination of cysteine residues was replaced by alanine residues (C47A, C101A, C47A/C101A, C14A/C47A/C10

114 rategy predicts that a viable HIV clone with alanine residues can genetically dominate the viral popu

115 Substitution of N353 and D354 in loop I with alanine residues caused the loss of binding ability and

116 can be attributed to the methyl group of the alanine residue clashing with a critical carboxyl group

117 , and substitution of these amino acids with alanine residues completely abolished the Ang II-induced

118 that a mutation of the KRK cluster to three alanine residues completely blocked the localization of

119 SNP at position 83 in the protein changes an alanine residue, conserved in NHLH2 orthologs through th

120 hemical identities of the tryptophan and two alanine residues contained in pacidamycin D have been sh

121 atives with individual cysteines replaced by alanine residues (Cys-->Ala).

122 by an amino acid substitution for one of the alanine residues (D for A at position 45 [A45D]), by del

123 d the aspartic and glutamic acid residues to alanine residues (DE572AA) lacked ATPase and helicase ac

124 At pH 4.0, replacing His12 with an alanine residue decreases the stability of the complex w

125 or other extracellular-facing histidines to alanine residues did not abolish the pH dependence of Zn

126 at the replacement of Trp(52) and Asp(55) by alanine residues diminished the growth inhibitory activi

127 uilt from BPTI sequences that contained many alanine residues distributed throughout the protein.

128 h both Trp52/Arg52 and Asp55 are replaced by alanine residues, do not have TGF-beta antagonist activi

129 Yet, replacing Asp121 with an asparagine or alanine residue does not have a substantial effect on ei

130 Replacing Asp121 with an asparagine or alanine residue does not perturb the overall conformatio

131 f these cysteine-serine-glutamate repeats by alanine residues does not similarly affect H2O2 and diam

132 sion of these serines to nonphosphorylatable alanine residues enhances cell migration, whereas their

133 mportantly, mutation of these three sites to alanine residues enhances the transcriptional activity o

134 of a single helical turn in the form of four alanine residues following Ile339 at the carboxyl termin

135 te three mutants of BsPFK that substitute an alanine residue for Glu-161, Arg-162, or both.

136 Substitution of an alanine residue for tyrosine at position 1424 significan

137 of PTE with the substitution of glycine and alanine residues for Ile-106, Phe-132, and/or Ser-308 re

138 Substitution of alanine residues for specific amino acids in the adjacen

139 notype was also demonstrated by substituting alanine residues for three of the five tryptophan residu

140 A peptide with two alanine residues formed laterally aligned fibrillar aggr

141 sidues (FosD) to mimic phosphorylation or to alanine residues (FosA) to prevent phosphorylation.

142 idine in the delta subunit is replaced by an alanine residue found at the homologous position in gamm

143 Two alanine residues from Arp7A that occupy equivalent apola

144 ucing a sequence containing only glycine and alanine residues (GAr) into substrates can impair their

145 aining either an aspartic acid (gBAsp900) or alanine residue (gBAla900) substitution at Ser900 to mim

146 s within this motif with nonphosphorylatable alanine residues generated a mutant Cactus that still fu

147 substitution of Syt-II Phe47 and Phe55 with alanine residues had little effect on the binding of BoN

148 J(HN alpha) coupling constants of individual alanine residues have been measured from 2 to 56 degrees

149 The alanine residues have then been progressively substitute

150 Alteration of S138 to an alanine residue impaired T-cell activation-induced ubiqu

151 However, the replacement of a single alanine residue in CG 117-136 with arginine, but not glu

152 id residue at each of these positions for an alanine residue in order to distinguish important from u

153 the substitution of proline for a conserved alanine residue in the carboxyl terminus of the goat mus

154 ese mutants (+1Ala) with an insertion of one alanine residue in the hinge region is partially deficie

155 the side chain of threonine 877, which is an alanine residue in the mutant.

156 To achieve this goal, a specific alanine residue in the parent protein is replaced with c

157 des that contain preferentially a proline or alanine residue in the second amino acid sequence positi

158 amino acids from peptides with a proline or alanine residue in the second position from the amino en

159 Substitution of the alanine residue in the third repeat (A249) with a charge

160 d the conserved serines were also changed to alanine residues in an EBNA-LP with two repeats, which i

161 Here we report the presence of 6-7 A-alanine residues in an endopeptidase of Streptococcus py

162 (125)I-Fc, whereas uptake by FcRn containing alanine residues in place of both Trp-311 and the dileuc

163 ic region, we have individually replaced the alanine residues in positions 5 and 7 with smaller (glyc

164 ee alanine was used to correct enrichment in alanine residues in protein and calculate the rate of pr

165 Replacement of six histidines by alanine residues in putative zinc binding sites of a KIR

166 lace serine 121 with cysteine, threonine, or alanine residues in the BSOR sequence to asses the role

167 e three motifs were individually replaced by alanine residues in the full-length kinase.

168 rizing serine, cysteine, O-methylserine, and alanine residues in the middle of peptide chains, thereb

169 ine-scanning mutagenesis at 16 of the 18 non-alanine residues in the sequence KEALQIKYNFSF RYIYPLD.

170 Mutating three conserved alanine residues in the tether region of the iron-sulfur

171 enylalanine, serine, tyrosine, threonine and alanine residues in TMs and ILs/ELs were detected.

172 Alanine residues in two model peptides, the pentapeptide

173 mTR3 is quite accommodating to insertion of alanine residues into the Cys-Sec dyad, with only a 4-6-

174 nd, the (13)C(alpha) CSA tensor of the first alanine residue is entirely different from that of the s

175 equence of the RXL motif is replaced by four alanine residues is largely refractory to inactivation b

176 eplacement of the Drosophila glutamine by an alanine residue leads to the complete loss of glycosylas

177 Deletion of an alanine residue lowers the activity by approximately 40%

178 Replacing Pca1 with an alanine residue lowers the catalytic activity of ONC by

179 Addition of two alanine residues lowers the activity by 90% and the appa

180 lix alpha-aneurysm with a downwards-directed alanine residue markedly interfered with signal transfer

181 e remodeling involving the small hydrophobic alanine residue may promote ATP synthesis by lowering th

182 Insertion of one to five alanine residues near either the N or C terminus of the

183 s highly conserved region, was mutated to an alanine residue, no enzymatic activity was detected.

184 residue or a phosphorylation-dead mimicking alanine residue nor deletion of prkC or prpC altered the

185 ed alpha-amino methylation of the N-terminal alanine residue of HRP II.

186 Previous work has shown that the N-terminal alanine residue of Sir3 (Ala2) and its acetylation play

187 of a D-methyl substituent on the penultimate alanine residue of the cell wall peptide.

188 he D-methyl substituent of the penultimate D-alanine residue of the DD-peptidase substrate.

189 Mechanistically, IAFGP binds the terminal d-alanine residue of the pentapeptide chain of bacterial p

190 e-directed mutagenesis of the amino-terminal alanine residue of this region to an aspartic acid (A78D

191 R chemical shifts of (13)C=O labeled central alanine residues of peptides W-Lys(5)-(t)L(3)-Ala(n)-(t)

192 y different from that of the second or later alanine residues of the peptide.

193 The N-terminal alanine residues of the silencing protein Sir3 and of Or

194 Three different alanine residues, one involved in backbone contacts, one

195 acid, which was either a serine residue, an alanine residue or deleted.

196 OOH termini by a high content of proline and alanine residues organized in an (AP)n motif.

197 Although the methyl groups of the alanine residues pack at their optimum van der Waals con

198 a), we mutated serine 643 of PKC-delta to an alanine residue (PKC-deltaS643A).

199 ]RKLANQ-NH2), which contains two constrained alanine residues (positions 7 and 11) and a C-terminal a

200 ituting all 4 wild-type lysine residues with alanine residues, prevented conversion to PrP(Sc).

201 tyrosines 474, 476, 477, and 480 mutated to alanine residues produced a gK-null-like phenotype chara

202 in the ATPase was changed to a serine or an alanine residue, producing strains C148S and C148A, C376

203 ich the Thr and Ser residues were changed to alanine residues, reduced the apoptotic activity of BIK

204 nase II phosphorylation site were changed to alanine residues, reducing phosphorylation by 70 to 80%.

205 dues (aa15, aa78, and aa82) were replaced by alanine residues, rendering them incapable of phosphoryl

206 lamblia enzyme, which when replaced with an alanine residue renders the enzyme inactive.

207 s were also constructed, e.g. asparagine and alanine residues replaced the native glycine with the re

208 , D207, E215, N283, and Q285 were changed to alanine residues; residues E299 and R303 to glutamine; a

209 binding site were replaced by a glycine and alanine residue, respectively.

210 osphorylation site (T1503) by mutation to an alanine residue resulted in greatly decreased GTP-bindin

211 tion of TasA fibers, as their replacement by alanine residues resulted in only minor defects in biofi

212 Replacing Asp121 with an asparagine or alanine residue results in a loss of conformational stab

213 ng the MAP kinase phosphorylation sites with alanine residues results in an increase in the binding a

214 of eNOS in which serine 116 is changed to an alanine residue (S116A) shows significantly enhanced enz

215 e residues in two RGD motifs were changed to alanine residues showed the greatest reduction in virule

216 Mutation of Arg-208 or Thr-207 to an alanine residue significantly altered the relative phosp

217 es at position 6, 12, and 21 to an uncharged alanine residue significantly increased transcriptional

218 ng replacement of Avr3a RXLR-EER motifs with alanine residues, singly or in combination, or with resi

219 The presence of a solvent-exposed alanine residue stabilizes a helix by 0.4-2 kcal.mol(-1)

220 n of S286 and S301 with non-phosphorylatable alanine residues strongly attenuates DNA damage-induced

221 An alanine residue, substituting for the arginine residue i

222 We screened a collection of single alanine residue substitution mutants spanning the entire

223 Independent serine-to-alanine residue substitutions in full-length STAT5A reve

224 Substitution of Thr-55 with an alanine residue (T55A) stabilizes p53 and impairs the ab

225 ffected individuals identified a total of 24 alanine residues that resulted from a duplication of nin

226 ne phosphorylation site was replaced with an alanine residue, the level of phosphorylation of the gI

227 expansions in the third repeat of 11 and 12 alanine residues, the latter being the largest expansion

228 However, mutagenesis of the alanine residue to cysteine did not confer thiol activat

229 Replacement of the alpha-proton of an alanine residue to generate alpha-aminoisobutyric acid (

230 Addition of an alanine residue to the flexible linker region of the Rie

231 in via vibrational spectroscopy, using cyano-alanine residues to form the anesthetic binding cavity.

232 The substitutions are of analogous alanine residues to glycine in each repeat, and allow th

233 lix pair exposes other conserved glycine and alanine residues to the fatty acyl environment, which ar

234 A sequence of seven alanine residues-too short to form an alpha-helix and wh

235 Gly-Thr-Asp-Arg-Val, can be substituted with alanine residues utilizing site-directed mutagenesis.

236 A beta-alanine residue was coupled to the main-chain (alpha) am

237 ereas an analogous compound lacking the beta-alanine residue was strongly localized in the nuclei of

238 because of mutagenesis of Cys21 and Cys24 to alanine residues was assembled into the complex and had

239 A BPTI mutant containing 22 alanine residues was further shown by 2D and 3D-NMR to f

240 le mutant, with all three Cys replaced by an alanine residue, was still palmitylated and remained mem

241 DmTR and CeTR2 was reduced 100-300-fold when alanine residues were inserted into the Cys-Cys dyad.

242 A mutant EBNA-3A protein in which alanine residues were substituted for amino acids 199, 2

243 The mutation of I106, F132, or S308 to an alanine residue, which enlarges the small or leaving gro

244 The sixth mutation replaces a surface alanine residue with phenylalanine.

245 raction of the leaving group, the terminal D-alanine residue, with the two enzymes was interesting, s

246 The synthetic peptide (Aib142) replaces an alanine residue within the V3 loop epitope sequence GPGR

247 e this tyrosine residue was replaced with an alanine residue (Y796A) had a nearly 8-fold decrease in

248 id 227 through asparagine 237) with a single alanine residue yields an alphaT subunit that fully bind