1 otein inhibitors and was further verified by
alanine scanning.
2 n interactions using computational interface
alanine scanning.
3 am positive pathogens was identified through
alanine scanning.
4 mplex, we targeted 20 positions in Prp28 for
alanine scanning.
5 GHRP-6 leads, which were further examined by
alanine scanning.
6 The binding epitope was determined with
alanine scanning.
7 In previous work using
alanine scanning (
1), we identified residues Leu-53, Asp
8 Using an
alanine-scanning 3A mutant, we show that Golgi targeting
9 n trafficking motifs in the channel and with
alanine scanning across aa 610-620.
10 We performed an
alanine scanning analyses of 35 hGHv residues and determ
11 Using 167 sequences from a shotgun
alanine scanning analysis of Site1, we have determined t
12 fected HEK-293 cells, we demonstrate through
alanine scanning and amino acid substitutions that the p
13 and sequence, and it provides computational
alanine scanning and change in solvent-accessible surfac
14 Alanine scanning and conservative substitutions identifi
15 Structure-function analysis of Ecm1 by
alanine scanning and conservative substitutions identifi
16 and HhH domains of Escherichia coli LigA by
alanine scanning and conservative substitutions, entaili
17 Alanine scanning and crystallographic structural analysi
18 a core determinant defined using a series of
alanine scanning and deletion mutant variants.
19 inding, as well as computational analysis of
alanine scanning and DMC data.
20 cin DNases through a combination of E9 DNase
alanine scanning and double-mutant cycles (DMCs) coupled
21 bed via standard and nonstandard techniques (
alanine scanning and hydrophile scanning, respectively),
22 ution of critical residues identified by the
alanine scanning and NMR spectroscopy, along with the el
23 might contact RNA polymerase, we carried out
alanine scanning and random mutagenesis of oxyR.
24 lix 3 of EntB-ArCP were generated by shotgun
alanine scanning and selected for their ability to suppo
25 We generated
alanine scanning and single and multiple amino acid subs
26 rved amino acids of Escherichia coli LigA by
alanine scanning and thereby identified five new residue
27 We have now employed a combination of
alanine-scanning and deletion mutagenesis to identify th
28 Using the Rosetta
alanine-scanning and design algorithms to predict destab
29 We have developed a novel computational
alanine scanning approach that involves analysis of ense
30 Alanine-scanning assays suggested the absence of known h
31 Alanine scanning-
based shotgun mutagenesis epitope mappi
32 Lyp substrate recognition using an "inverse
alanine scanning"
combinatorial library approach.
33 scence polarization displacement assays, and
alanine scanning data all suggest that they bind to the
34 Alanine scanning defined seven individual amino acids as
35 Alanine scanning demonstrated that the epitope involves
36 idging may explain the previous finding from
alanine scanning experiments that R333 contributes signi
37 s is also in line with systematic, in silico
Alanine scanning free-energy simulations, which indicate
38 is using recombinant proteins and a panel of
alanine-scanning GPC mutants revealed that F100G5 bindin
39 Alanine scanning helped resolve the boundaries of this c
40 OS-PDZ complex that correlated well with the
alanine scanning identified regions of dystrophin.
41 Through
alanine scanning,
immunofluorescence cell staining and c
42 Using shotgun
alanine scanning in conjunction with this selection, we
43 Alanine scanning insertion mutagenesis was used to exami
44 Mutating residues into alanine (
alanine scanning)
is one of the fastest experimental mea
45 Using this plasmid, an
alanine scanning library has been constructed and the mu
46 To do so, we created a tri-
alanine scanning library that covers all of sigma(70) R4
47 activities are underscored by the results of
alanine-scanning,
limited proteolysis, and deletion anal
48 he hot spot residues known from experimental
alanine scanning measurements.
49 Using
alanine scanning,
molecular dynamics simulations, and tr
50 Alanine scanning mutagenesis analysis shows that four co
51 kappaB pathway signaling, using experimental
alanine scanning mutagenesis and also the FTMap method f
52 Using
alanine scanning mutagenesis and crystallographic approa
53 cid residues of a prototypic PPPSP motif via
alanine scanning mutagenesis and demonstrate that each o
54 LIAGCITSTDPVLSALI(152)) in activity by using
alanine scanning mutagenesis and examining salt toleranc
55 Alanine scanning mutagenesis and kinetic analysis reveal
56 We performed saturated
alanine scanning mutagenesis and other amino acid substi
57 5) to envelope function has been examined by
alanine scanning mutagenesis and subsequent characteriza
58 In this work, we have used an
alanine scanning mutagenesis approach to identify whethe
59 the vinylogous urea binding site, horizontal
alanine scanning mutagenesis between p51 residues Lys-27
60 Alanine scanning mutagenesis confirms that these residue
61 o and TgRON2L1-bound forms complemented with
alanine scanning mutagenesis data reveal an unexpected a
62 Alanine scanning mutagenesis demonstrated that positivel
63 Alanine scanning mutagenesis determined that the key res
64 Alanine scanning mutagenesis in sGC indicates that the H
65 Using
alanine scanning mutagenesis in the activity subdomain o
66 Alanine scanning mutagenesis maps a novel epitope to a s
67 Alanine scanning mutagenesis of 12 Arg/Lys residues of e
68 We thus performed extensive
alanine scanning mutagenesis of A1AR-ECL2 to explore the
69 rformed the first complete charge-cluster-to-
alanine scanning mutagenesis of an ARP and compared the
70 Alanine scanning mutagenesis of areas flanking Leu(172),
71 In this work, we performed
alanine scanning mutagenesis of aromatic residues locate
72 We performed
alanine scanning mutagenesis of AuIB and alpha3beta4 nAC
73 To test this possibility, we employed
alanine scanning mutagenesis of CB1 EC2 and identified t
74 Alanine scanning mutagenesis of conserved amino acid res
75 Charged-to-
alanine scanning mutagenesis of CSM4 yielded one allele,
76 Alanine scanning mutagenesis of hVbeta2.1 wild-type and
77 ctions and receptor activation, we performed
alanine scanning mutagenesis of loop residues and assess
78 Using
alanine scanning mutagenesis of MBM1, we found that the
79 Alanine scanning mutagenesis of MPER indicates that it h
80 nd cofactor activity were assessed following
alanine scanning mutagenesis of residues 555-571 that bo
81 Alanine scanning mutagenesis of residues 64-70, within f
82 Alanine scanning mutagenesis of the alpha-region reveals
83 Alanine scanning mutagenesis of the C-terminal region of
84 Alanine scanning mutagenesis of the conserved residues o
85 In this study, we performed
alanine scanning mutagenesis of the ExsA alpha-helix (re
86 of chimeras between these OCLN proteins and
alanine scanning mutagenesis of the extracellular domain
87 Alanine scanning mutagenesis of the LIM interaction doma
88 Here, we report the results of saturating
alanine scanning mutagenesis of the N-terminal domain of
89 f recombinant mutant CSFVs was created using
alanine scanning mutagenesis of the p7 gene harboring se
90 We describe
alanine scanning mutagenesis of the PhoP alpha loop and
91 random mutagenesis results, we performed an
alanine scanning mutagenesis of the TLR2 DD loop and par
92 Alanine scanning mutagenesis of this region revealed tha
93 We performed
alanine scanning mutagenesis of this region, and we quan
94 Alanine scanning mutagenesis of three to five consecutiv
95 Towards this end, we performed
alanine scanning mutagenesis on selected residues in the
96 ole of NS4A in these processes, we conducted
alanine scanning mutagenesis on the C-terminal acidic do
97 Alanine scanning mutagenesis revealed a requirement for
98 Alanine scanning mutagenesis revealed four classes of mu
99 Alanine scanning mutagenesis revealed that amino acids K
100 Alanine scanning mutagenesis revealed that the K/R-R/x-x
101 Alanine scanning mutagenesis studies revealed that the T
102 e, we performed a comprehensive deletion and
alanine scanning mutagenesis study of this protein in th
103 (ATR) and curcumin (CCM), here we perform an
alanine scanning mutagenesis study.
104 ides that bound Gbetagamma were subjected to
alanine scanning mutagenesis to determine their relevanc
105 We used
alanine scanning mutagenesis to examine the contribution
106 We have used structure-directed
alanine scanning mutagenesis to identify determinants in
107 ther analyzed by combining triple and single
alanine scanning mutagenesis to identify individual resi
108 We used NMR experiments and
alanine scanning mutagenesis to identify residues in the
109 In this study, we used
alanine scanning mutagenesis to identify the key residue
110 We then used
alanine scanning mutagenesis to locate the epitopes.
111 We used
alanine scanning mutagenesis to replace selected amino a
112 We used
alanine scanning mutagenesis to study the contributions
113 The current study used
alanine scanning mutagenesis to understand the selectivi
114 motif centered around the sequence "SQELD."
Alanine scanning mutagenesis was carried out on the T(35
115 Alanine scanning mutagenesis was performed for the resid
116 Systematic
alanine scanning mutagenesis was performed on the substr
117 Alanine scanning mutagenesis was performed to assess the
118 s parainfluenza virus 5 F protein TM domain,
alanine scanning mutagenesis was performed.
119 In this study,
alanine scanning mutagenesis was used in combination wit
120 Alanine scanning mutagenesis was used to determine the r
121 Alanine scanning mutagenesis was used to map the specifi
122 Alanine scanning mutagenesis within the region of amino
123 Using in vitro assembly,
alanine scanning mutagenesis, and biophysical analyses,
124 Using an in vitro assembly assay,
alanine scanning mutagenesis, and biophysical techniques
125 Using computational docking algorithms,
alanine scanning mutagenesis, and surface plasmon resona
126 side chains at this interface, identified by
alanine scanning mutagenesis, are conserved among DSX ho
127 probed the in vivo role of the linker using
alanine scanning mutagenesis, assaying stressosome outpu
128 ude random peptide phage display mapping and
alanine scanning mutagenesis, to identify residues in th
129 Using
alanine scanning mutagenesis, we demonstrate that the bi
130 Using
alanine scanning mutagenesis, we show that a pore helix
131 Using
alanine scanning mutagenesis, we show that two distinct
132 gp41 HR1 has been systematically examined by
alanine scanning mutagenesis, with subsequent characteri
133 esults from loss-of-binding studies using an
alanine scanning mutagenesis-based epitope mapping appro
134 bution of the arm to folding was obtained by
alanine scanning mutagenesis.
135 2 cytoplasmic tail were identified by single-
alanine scanning mutagenesis.
136 helix 11, Thr978, as an essential residue by
alanine scanning mutagenesis.
137 GABPbeta interface residues were chosen for
alanine scanning mutagenesis.
138 nal mutagenesis, carbohydrate shielding, and
alanine scanning mutagenesis.
139 We subjected HD5 to comprehensive
alanine scanning mutagenesis.
140 ng of the epitopes was accomplished by using
alanine scanning mutagenesis.
141 Deletion or
alanine- scanning mutagenesis through this domain signif
142 nteracts with GP, here we used comprehensive
alanine-scanning mutagenesis (shotgun mutagenesis), neut
143 Here, we perform
alanine-scanning mutagenesis across CDR H3 and make addi
144 explore this molecular environment, we used
alanine-scanning mutagenesis across residues 660 to 680
145 Alanine-scanning mutagenesis along the entire length of
146 L binding site of AFABP/aP2 a combination of
alanine-scanning mutagenesis and fluorescence resonance
147 ity binding at different DNA sequences using
alanine-scanning mutagenesis and identified several key
148 Alanine-scanning mutagenesis and kinetic analysis identi
149 Peptide
alanine-scanning mutagenesis and modeling of receptor-bo
150 We used
alanine-scanning mutagenesis and patch clamp photometry
151 pes on VEGF for these three antibodies using
alanine-scanning mutagenesis and structural analyses.
152 1) to envelope function has been examined by
alanine-scanning mutagenesis and subsequent characteriza
153 lex as a guide, we undertook a comprehensive
alanine-scanning mutagenesis approach at the TCR-pMHC-I
154 tersubunit interactions of K33, we performed
alanine-scanning mutagenesis at charged residues in the
155 of flat beta-sheets, we performed extensive
alanine-scanning mutagenesis experiments on the single-l
156 Isolated A2 was subjected to
alanine-scanning mutagenesis followed by expression of a
157 Alanine-scanning mutagenesis identified a functional TIL
158 Alanine-scanning mutagenesis identified an acidic-residu
159 We have performed surface acidic-to-
alanine-scanning mutagenesis of 3C to identify the surfa
160 Alanine-scanning mutagenesis of 48 amino acids combined
161 In this report we have used
alanine-scanning mutagenesis of a putative coiled coil a
162 Alanine-scanning mutagenesis of B. subtilis alphaCTD unc
163 Alanine-scanning mutagenesis of DC-SIGN revealed that hi
164 Alanine-scanning mutagenesis of FNR amino acid residues
165 Alanine-scanning mutagenesis of HVEM was used to further
166 Results from
alanine-scanning mutagenesis of hydrophobic residues in
167 Alanine-scanning mutagenesis of predicted catalytic resi
168 Alanine-scanning mutagenesis of residues predicted to li
169 Alanine-scanning mutagenesis of rho-TIA showed F18A had
170 he same TCR, complemented with computational
alanine-scanning mutagenesis of SEA, SEB, SEC3, SEE, and
171 Using
alanine-scanning mutagenesis of sigma32 and in vivo and
172 ermore, we report the solution structure and
alanine-scanning mutagenesis of TACI_d2 along with co-cr
173 pe residues to CNTO607 binding, we performed
alanine-scanning mutagenesis of the A-D region of IL-13.
174 We combined
alanine-scanning mutagenesis of the A1AR second extracel
175 Alanine-scanning mutagenesis of the alternatively splice
176 Alanine-scanning mutagenesis of the ankyrin-B ANK (ankyr
177 ding determinants were identified by shotgun
alanine-scanning mutagenesis of the BR3 ECD expressed on
178 Alanine-scanning mutagenesis of the GLUT4 amino terminus
179 Alanine-scanning mutagenesis of the intracellular C term
180 Alanine-scanning mutagenesis of the Na(v)1.6 N terminus
181 rlying biochemical mechanism by carrying out
alanine-scanning mutagenesis of the PKR activation domai
182 In this study, we used
alanine-scanning mutagenesis of the residues between C13
183 Alanine-scanning mutagenesis of the TMH identified sever
184 Alanine-scanning mutagenesis of these nine side chains s
185 We performed
alanine-scanning mutagenesis of this motif ((14)TFPLF(18
186 Alanine-scanning mutagenesis of this region suggested th
187 Here, we performed comprehensive
alanine-scanning mutagenesis on csrA of E. coli and test
188 In this report, we conduct
alanine-scanning mutagenesis on the 14 other conserved s
189 s) on gp120, and its footprint as defined by
alanine-scanning mutagenesis overlaps that of b12.
190 Alanine-scanning mutagenesis produced one rCPE variant,
191 Alanine-scanning mutagenesis revealed five additional re
192 Alanine-scanning mutagenesis revealed that an F protein
193 Alanine-scanning mutagenesis revealed that residues 1773
194 Alanine-scanning mutagenesis revealed that residues Phe4
195 Alanine-scanning mutagenesis revealed that the affinitie
196 Alanine-scanning mutagenesis revealed the molecular basi
197 containing mutations was generated using an
alanine-scanning mutagenesis strategy.
198 Alanine-scanning mutagenesis studies targeting residues
199 Here we report an
alanine-scanning mutagenesis study of the Runt domain th
200 have contact residues within aa 412 to 423,
alanine-scanning mutagenesis suggested that one subset,
201 te in CA for Ubc9 was mapped by deletion and
alanine-scanning mutagenesis to a consensus motif for SU
202 orescence correlation spectroscopy (FCS) and
alanine-scanning mutagenesis to characterize the interac
203 In this work, we used
alanine-scanning mutagenesis to elucidate the structural
204 In this work we use
alanine-scanning mutagenesis to explore the contribution
205 We used
alanine-scanning mutagenesis to identify regions of huma
206 This study applied
alanine-scanning mutagenesis to investigate the role of
207 First, 32
alanine-scanning mutagenesis variants of dystrophin R16-
208 Alanine-scanning mutagenesis was applied to complete the
209 Alanine-scanning mutagenesis was applied to the correspo
210 Alanine-scanning mutagenesis was previously performed to
211 Alanine-scanning mutagenesis was previously performed to
212 Alanine-scanning mutagenesis was used to confirm that th
213 In this study, charged to
alanine-scanning mutagenesis was used to generate condit
214 Alanine-scanning mutagenesis was used to identify YscF m
215 Alanine-scanning mutagenesis was used to investigate the
216 Using
alanine-scanning mutagenesis we found four residues, all
217 Through extensive deletion and
alanine-scanning mutagenesis we have mapped key residues
218 By performing
alanine-scanning mutagenesis we identified a dilysine se
219 For this study, by employing
alanine-scanning mutagenesis, (125)I-SDF-1alpha competit
220 ccompanying article, we demonstrate, through
alanine-scanning mutagenesis, a key role for extracellul
221 l residues were determined in two designs by
alanine-scanning mutagenesis, and are consistent with th
222 by a combination of cysteine cross-linking,
alanine-scanning mutagenesis, and computational simulati
223 d its membrane binding properties, performed
alanine-scanning mutagenesis, and identified residues im
224 Using
alanine-scanning mutagenesis, in cellulo bioluminescence
225 Using
alanine-scanning mutagenesis, loss-of-function recombina
226 Using
alanine-scanning mutagenesis, we have made mutations in
227 Using
alanine-scanning mutagenesis, we identified two clusters
228 Using
alanine-scanning mutagenesis, we probed the roles of two
229 G1 and G2, we performed point mutations and
alanine-scanning mutagenesis.
230 im101 and ESCRT pathways, which we tested by
alanine-scanning mutagenesis.
231 the residues within this loop, we performed
alanine-scanning mutagenesis.
232 tide and peptide agonists were studied using
alanine-scanning mutagenesis.
233 e-chains to GrpE-DnaK binding were probed by
alanine-scanning mutagenesis.
234 the control of specific traits, we performed
alanine-scanning mutagenesis.
235 f conserved switch II residues by performing
alanine-scanning mutagenesis.
236 s explored by cross-competition analysis and
alanine-scanning mutagenesis.
237 we generated the entire histone H2A and H2B
alanine-scanning mutant strains in another background, w
238 pare the kinetic rates and affinities for 18
alanine scanning mutants comprising epitope residues 50-
239 Binding of X5 to
alanine scanning mutants of gp120JR-CSF complexed with C
240 species variants, (2) species chimeras, (3)
alanine scanning mutants, and (4) site-specific mutants.
241 we measured their binding to a panel of 11 G
alanine-scanning mutants and identified two mutants, P18
242 We constructed a t-toxin library of ProTx-I
alanine-scanning mutants and screened this library again
243 In addition, it was found that when the BLIP
alanine-scanning mutants were tested in the strain, the
244 -sulfated analogues, truncated peptides, and
alanine-scanning mutants, suggested that each of the 12-
245 Alanine scanning mutation of Epep revealed residues crit
246 We describe here an
alanine scanning mutational analysis of the Abeta(1-40)
247 Alanine-scanning mutational analysis of the first 62 ami
248 ain by single amino acid substitutions and 3-
alanine scanning mutations identified important but not
249 A series of
alanine scanning mutations of hot-spot residues at the i
250 Most of the
alanine-scanning mutations in the CT had little effect o
251 rotein (CP) rescued RNA synthesis by several
alanine-scanning mutations in the N-terminal alpha helix
252 of glycoprotein incorporation, we introduced
alanine-scanning mutations into this region of the trans
253 function has been systematically examined by
alanine scanning of all gp41 loop residues and the subse
254 Alanine scanning of AP-Cav-B revealed that Thr-90 and -9
255 Second, shotgun
alanine scanning of BCMA was used to map critical residu
256 Finally,
alanine scanning of CDR1 and CDR2 sequences of TRBV4-1 r
257 Alanine scanning of GpTx-1 revealed that residues Trp(29
258 Comparative
alanine scanning of Im2 and Im9 residues involved in bin
259 Alanine scanning of MPER residues 664 to 680 revealed th
260 Alanine scanning of the active-site residues shows that
261 ucture-activity relations of the Spt5 CTD by
alanine scanning of the consensus nonapeptide.
262 Alanine scanning of the E6AP-UbcH7 binding interface ide
263 ar determinants of interaction, we performed
alanine scanning of the Kv4.3 S3b region.
264 Alanine scanning of the last five residues revealed the
265 Alanine scanning of the loops surrounding the protease a
266 Alanine scanning of the peptide sequence, combined with
267 Furthermore,
alanine scanning of the RBD has identified several resid
268 Alanine scanning of their complementarity-determining re
269 Alanine scanning of this interface has identified the ho
270 n to a previously described "DXL" motif, the
alanine scanning results predicted four amino acid posit
271 Alanine scanning revealed that hydrophobic amino acids i
272 of the TCR-peptide-HLA ternary complexes and
alanine scanning revealed that the autologously derived
273 of UBL-containing proteins and IKKbeta, and
alanine scanning revealed that the leucine at position 3
274 Furthermore,
alanine scanning reveals modest differences in the ssDNA
275 Alanine scanning reveals residues crucial for GEF activi
276 An unbiased
alanine-scanning screen covering the entire region combi
277 We identify by
alanine scanning seven functionally important amino acid
278 ium exchange mass spectrometry (H/DXMS), and
alanine scanning site-directed mutagenesis.
279 Alanine scanning studies of the secreted recombinant rec
280 We found from exhaustive
alanine scanning studies that fibrillation of this WW do
281 analyzing the binding of Brd4 to a series of
alanine-scanning substitution mutants of the human papil
282 Alanine-scanning substitution of this 14 amino acid regi
283 characterized using overlapping peptides and
alanine scanning substitutions and were localized to two
284 itope for gp120, as previously identified by
alanine scanning substitutions on the gp120 surface.
285 Here, we used
alanine scanning substitutions spanning residues 1023 to
286 stigate the bioactive surface of ProTx-II by
alanine-scanning the toxin and analyzing the interaction
287 box C/D small RNP complex, we have employed
alanine scanning to evaluate the interaction between the
288 We have performed computational
alanine scanning to gain insight into thermodynamic aspe
289 Finally, we used
alanine scanning to identify determinants in the C-termi
290 ting affinities of protein variants (shotgun
alanine scanning)
to analysis of GB1 stability.
291 Computational
alanine scanning using the molecular mechanics Poisson-B
292 ssays for receptor binding and calcium flux,
alanine-scanning variants of nociceptin indicated that f
293 Computational
alanine scanning was also conducted to identify putative
294 Each of the 23 BLIP positions examined by
alanine scanning was randomized to create libraries cont
295 Alanine scanning was used to construct mutants of hOAT1,
296 Computational
alanine scanning was used to identify key residues in th
297 Combinatorial shotgun
alanine-scanning was used to assess intramolecular coope
298 Using
alanine scanning,
we demonstrated that Leu-127 and Leu-1
299 By
alanine scanning,
we identified a single mutation in the
300 p these determinants, we performed global E2
alanine scanning with a panel of 16 human monoclonal ant