ライフサイエンスコーパス: aldolase

1 defect is rescued by expression of exogenous aldolase.

2 retains the known F-actin binding ability of aldolase.

3 hoglucose isomerase, phosphofructokinase and aldolase.

4 ate dehydrogenase, malate dehydrogenase, and aldolase.

5 substrate tolerance is unprecedented for an aldolase.

6 id changes from the wild-type E. coli KDPGal aldolase.

7 olase, and 2-keto-3-deoxy-6-phosphogluconate aldolase.

8 a representative member of this family, FBP aldolase.

9 rded as an organocatalytic mimic of fuculose aldolase.

10 5Ac (N-acetylneuraminic acid, D-sialic acid) aldolase.

11 d 2-keto-3,6-dideoxy-6-sulfogluconate (KDSG) aldolase.

12 cose is split into 3-carbon intermediates by aldolase.

13 Schiff base with the substrate, unlike most aldolases.

14 ual N-terminal extensions not found in other aldolases.

15 cement that rivals the efficiency of class I aldolases.

16 s involved being parvalbumins, enolases, and aldolases.

17 ases yet similar to metal-dependent class II aldolases.

18 late intermediates (4-hydroxy-2-ketovalerate aldolase, 2-isopropylmalate synthase, and transcarboxyla

19 analysis we identified the glycolytic enzyme aldolase A (AldoA) as a binding partner of CAPN3.

20 ytic enzymes we evaluated by gene silencing, aldolase A (ALDOA) blockade produced the most robust dec

21 io of kcat/Km for the two substrates between aldolase A and aldolase B.

22 yruvate kinase, isoforms of creatine kinase, aldolase A and an isoform of glyceraldehyde 3-phosphate

23 o provide a preclinical rationale to develop aldolase A inhibitors as a generalized strategy to treat

24 with a specific small-molecule inhibitor of aldolase A was sufficient to increase overall survival i

25 we identified glycolytic enzymes (GAPDH and aldolase A) as putative interacting proteins.

26 skeleton, release of filamentous actin-bound aldolase A, and an increase in aldolase activity.

27 ur previously untargeted glycolytic enzymes, aldolase A, glyceraldehyde 3-phosphate dehydrogenase, tr

28 formational flexibility has been observed in aldolase A, its function in the catalytic reaction of al

29 phosphate dehydrogenase, myosin light chain, aldolase A, pyruvate kinase, glycogen phosphorylase, act

30 Constitutive ectopic expression of aldolases A and C accelerates the decay of a neurofilame

31 Aldolases A and C are glycolytic enzymes expressed in ne

32 Our findings strongly suggest that aldolases A and C are regulatory components of a light n

33 Interactions of aldolases A and C in NF-L expression may be linked to re

34 Aldolases A and C, but not B, interact specifically with

35 Fructose-1,6-bisphosphate (FBP) aldolase, a glycolytic enzyme, catalyzes the reversible

36 Aldolase, a glycolytic enzyme, must distinguish between

37 )beta3 and alpha(v)beta5, and the monoclonal aldolase Ab 38C2.

38 es underwent covalent crosscoupling with the aldolase Ab, which appears to be a limiting factor of th

39 small molecule drugs to mAbs in general and aldolase Abs in particular.

40 Knockdown of aldolases activates AMPK even in cells with abundant glu

41 , suggesting that both the plasticity of the aldolase active-site region and the multimeric nature of

42 tryptophan led to enzymes with no detectable aldolase activity.

43 confirming that it has fructose bisphosphate aldolase activity.

44 teins exhibited significant dihydroneopterin aldolase activity.

45 range of protein folds, had detectable retro-aldolase activity.

46 s actin-bound aldolase A, and an increase in aldolase activity.

47 LsrF, despite strong structural homology to aldolases, acts as a thiolase, an activity previously un

48 sing SAD phasing and belongs to the class II aldolase/adducin superfamily.

49 is and comparison of the enzyme with related aldolases, ADH synthase is classified as a new member of

50 Structural analysis revealed that both aldolases adopt a TIM barrel fold accessorized with dive

51 model posits that fructose-1,6-bisphosphate aldolase (ALD) provides a critical link between the cyto

52 -actin with muscle fructose-1,6-bisphosphate aldolase (aldolase) and glyceraldehyde-3-phosphate dehyd

53 pecificity among fructose-1,6-(bis)phosphate aldolase (aldolase) isozymes.

54 cleavage to pyruvate and L-lactaldehyde via aldolase and (iv) L-lactaldehyde conversion to L-lactate

55 or both of fructose 1,6-bisphosphate (F16BP) aldolase and 2-deoxyribose 5-phosphate (dR5P) aldolase (

56 ctural and biochemical analyses of T. gondii aldolase and aldolase-like proteins reveal diverse funct

57 Both aldolase and CAPN3 are present in the triad-enriched fra

58 Chicken parvalbumin and two new allergens, aldolase and enolase, were identified at 12, 40, and 50

59 FolB1 proteins all had both dihydroneopterin aldolase and epimerase activities, and carried out the a

60 ythritol as the sole C source should require aldolase and fructose-1,6-bisphosphatase to produce esse

61 Muscle aldolase and GAPDH showed low-affinity for binding yeast

62 ian dynamics simulations of yeast and muscle aldolase and GAPDH with yeast and muscle actin compared

63 nd aminoethanol using D-fructose-6-phosphate aldolase and L-rhamnulose-1-phosphate aldolase catalysts

64 The catalytic activities of aldolase and pyruvate kinase functionally modulate K(ATP

65 ing, respectively, fructose 1,6-bisphosphate aldolase and pyruvate kinase, under the same conditions.

66 Interaction between aldolase and SUR was confirmed using GST pulldown assays

67 nts shows that disruption of binding between aldolase and the B subunit of V-ATPase results in disass

68 ortant role for physical association between aldolase and V-ATPase in the regulation of the proton pu

69 te and dehydration of the Schiff base in FBP aldolase and, by analogy, the class I aldolase family.

70 ral feature of biological catalysts, such as aldolases and small molecule amine organocatalysts.

71 h muscle fructose-1,6-bisphosphate aldolase (aldolase) and glyceraldehyde-3-phosphate dehydrogenase (

72 tine kinase, aspartate aminotransferase, and aldolase) and inversely correlated with the duration of

73 n, glyceraldehyde-3-phosphate dehydrogenase, aldolase, and aspartate aminotransferase and thus reacti

74 d that the membrane binding sites for GAPDH, aldolase, and PFK reside on band 3, but related analyses

75 substrates of members of the PdxA, class II aldolase, and RuBisCO superfamilies are phosphorylated,

76 olase enzymes and recent studies on designed aldolase antibodies and organocatalysts, direct structur

77 gs were synthesized, and their activation by aldolase antibody (Ab) 38C2 was evaluated by DNA-cleavin

78 intermediate in the crystal structure of an aldolase antibody 33F12 in complex with a 1,3-diketone d

79 Interactions of SNX9 with aldolase are far more extensive and differ from those of

80 Crystals of AP-aldolase are grown at two temperatures (4 degrees C and

81 Intriguingly, both aldolases are competent to bind polymerized actin in vit

82 Thus, stereocomplementary class II pyruvate aldolases are now available to create chiral 4-hydroxy-2

83 mechanisms that direct the functions of the aldolase as a scaffold protein.

84 The identification of aldolases as methyl proteins in Arabidopsis and other sp

85 These results establish that aldolase, as well as being a glycolytic enzyme, is a sen

86 gesting that PABP shields the NF-L mRNA from aldolase attack.

87 ror in metabolism caused by mutations in the aldolase B gene, which is critical for gluconeogenesis a

88 tural and functional investigations of human aldolase B with the A149P substitution (AP-aldolase) hav

89 rase-cyclodeaminase, fructose-bisphosphatase aldolase B, sarcosine dehydrogenase, and cysteine sulfin

90 or the two substrates between aldolase A and aldolase B.

91 glycolytic enzyme fructose-1,6-bisphosphate aldolase (BB0445), the Borrelia oxidative stress regulat

92 more, TRAP and WASp, but not other unrelated aldolase binders, compete for the binding to the enzyme

93 mutant transgenes lacking mRNA sequence for aldolase binding are stabilized.

94 upstream acidic region is not necessary for aldolase binding but is nonetheless essential to parasit

95 y using alanine point mutants to investigate aldolase binding in vitro and to test functionality in t

96 Together, our results define a conserved aldolase binding motif in the WASp family members and su

97 findings along with the presence of similar aldolase binding motifs in additional human proteins, so

98 nultimate tryptophan, which is essential for aldolase binding, and clustered acidic residues.

99 ibits E. coli class II fructose bisphosphate aldolase, but not RNA polymerase.

100 to improve a previously optimized artificial aldolase by an additional factor of 30 to give a >10(9)

101 els of mRNAs encoding the glycolytic enzymes aldolase C (AldoC, also known as zebrin II) and phosphof

102 Moreover, the isoenzyme aldolase C [also known as zebrin II (ZII)] is heterogene

103 A specific in vivo interaction between aldolase C and NF-L mRNA had been localized to a 68 nt s

104 mpetitive interactions in cells coexpressing aldolase C and NF-L.

105 f mRNA decay has assessed mechanisms whereby aldolase C and PABP control NF-L expression.

106 The multifunctional proteins aldolase C and poly (A)-binding protein (PABP) undergo c

107 This model shows that aldolase C is a zinc-activated ribonuclease that cleaves

108 th their expression of the glycolytic enzyme aldolase C or zebrin.

109 n spinal cord pulls down the dimeric form of aldolase C suggesting that their co-regulation of NF-L e

110 cells that express high levels of zebrin II (aldolase C) and the glutamate transporter EAAT4 cluster

111 Zebrin II (ZII; a.k.a. aldolase C) is expressed heterogeneously in Purkinje cel

112 Zebrin II (aldolase C) is expressed in a subset of Purkinje cells i

113 ck protein 10 (Hsp10), fructose bisphosphate aldolase C, and NADH-ubiquinone oxidoreductase as protei

114 ase-related protein 2, fructose-bisphosphate aldolase C, chaperonin-containing T-complex polypeptide

115 in, complement C9, gelsolin, testican-2, and aldolase C, performed well in a training set (area under

116 with unrelated sequence, is not degraded by aldolase C.

117 d be linked to the oligomerization status of aldolase C.

118 hoglycerate kinase 1 (PGK 1), alpha enolase, aldolase C/Zebrin II) were included among the axonally s

119 The trans-o-hydroxybenzylidene pyruvate aldolase-catalysed reactions between fluoropyruvate and

120 sphate aldolase and L-rhamnulose-1-phosphate aldolase catalysts, respectively.

121 d on using the chemistry of the well studied aldolase catalytic antibodies of which mAb 38C2 is a mem

122 ymatically modified derivatives, sialic acid aldolase-catalyzed condensation reaction leads to the fo

123 In addition, a cascade of three aldolase-catalyzed reactions enables one-pot assembly of

124 BphI, a pyruvate-specific class II aldolase, catalyzes the reversible carbon-carbon bond fo

125 Of these enzymes, knockdown of aldolase causes the greatest effect, inhibiting cell pro

126 ata from top-down MS of native and denatured aldolase complexes, a total of 56% of the total backbone

127 Aldolase connects the motor actin filaments to transmemb

128 of glFBPA, whereas tagatose-1,6-bisphosphate aldolase contains an alanine in this position.

129 the homologous 2-dehydro-3-deoxygalactarate aldolase, coupled with site-directed mutagenesis data, i

130 The growth of T. gondii aldolase crystals in acidic conditions enabled trapping

131 pped in the active site of Toxoplasma gondii aldolase crystals to high resolution.

132 the homologous 2-dehydro-3-deoxygalactarate aldolase (DDGA).

133 d acceleration, with deoxyribose-5-phosphate aldolase (DERA) achieving an average 15-fold enhancement

134 ntermediate is produced using a deoxy ribose aldolase (DERA) enzyme in which two carbon-carbon bonds

135 chia coli) class I 2-deoxyribose-5-phosphate aldolase (DERA) has been determined by Se-Met multiple a

136 ldolase and 2-deoxyribose 5-phosphate (dR5P) aldolase (DERA).

137 the highest-resolution X-ray structure of an aldolase determined to date and enables a true atomic vi

138 (KDGP), which is subsequently cleaved by the aldolase DgaF to form glyceraldehyde-3-phosphate and pyr

139 The enzymes dihydroneopterin aldolase (DHNA) and 6-hydroxymethyl-7,8-dihydropterin py

140 Dihydroneopterin aldolase (DHNA) catalyzes the conversion of 7,8-dihydron

141 Dihydroneopterin aldolase (DHNA) catalyzes the conversion of 7,8-dihydron

142 e, as no candidate gene for dihydroneopterin aldolase (DHNA) could be identified.

143 The gene encoding 7,8-dihydroneopterin aldolase (DHNA) was recently identified in archaea throu

144 Yeast aldolase did not specifically bind to either yeast or mu

145 es of progressively larger substrates to the aldolase, DmpG, using molecular dynamics.

146 ins, a flavin reductase-like protein, and an aldolase, each located in thylakoid-associated plastoglo

147 ersion of Thr to Gly and acetaldehyde by Thr aldolase (EC 4.1.2.5) was only recently shown to play a

148 In contrast, aldolase enzymatic activity is not required for V-ATPase

149 We have used the aldolase enzyme as a model protein to conduct our studie

150 Detailed comparison with other class I aldolase enzymes and DERA enzymes from different organis

151 ades of investigation of naturally occurring aldolase enzymes and recent studies on designed aldolase

152 talytic antibody than speculated for natural aldolase enzymes and should serve to guide future studie

153 of the divalent metal ion dependent class II aldolase enzymes that have great biosynthetic potential.

154 In a fashion analogous to aldolase enzymes, the de novo preparation of L-ribulose,

155 Mimicking the actions of aldolase enzymes, the synthesis of selected carbohydrate

156 Aldolase exists as three isozymes, A, B, and C, distingu

157 the LC4 motif of human SNX9 in complex with aldolase explains the biochemistry and biology of this i

158 strategy consists of L-fuculose-1-phosphate aldolase F131A-variant-catalyzed aldol addition of dihyd

159 yase family has been included in the class I aldolase family on the basis of similar Schiff-base chem

160 ) is a member of the class II zinc-dependent aldolase family that catalyzes the cleavage of d-fructos

161 defined in the SCOPS database as the class I aldolase family.

162 in FBP aldolase and, by analogy, the class I aldolase family.

163 The class IIa fructose 1,6-bisphosphate aldolase (FBA) enzyme from M. tuberculosis (MtFBA) has b

164 ociation and included: fructose-bisphosphate aldolase (Fba); methyltetrahydropteroyltriglutamate (Met

165 dependent class II fructose-1,6-bisphosphate aldolase (FBA-tb), a key enzyme of gluconeogenesis absen

166 Fructose-1, 6-bisphosphate aldolases (FBA) are cytoplasmic glycolytic enzymes, whic

167 f nickel toxicity in E. coli as the class II aldolase FbaA through binding to the non-catalytic zinc

168 sults suggest that fructose-1,6-bisphosphate aldolase (FbaA) is a target of nickel toxicity.

169 Giardia lamblia fructose-1,6-bisphosphate aldolase (FBPA) is a member of the class II zinc-depende

170 ass I and class II fructose-1,6-bisphosphate aldolases (FBPA), glycolytic pathway enzymes, exhibit no

171 Dihydroneopterin aldolase (FolB) catalyzes conversion of dihydroneopterin

172 functionalization of the classic TIM barrel aldolase fold.

173 el equivalent to the wild-type D-sialic acid aldolase for its natural substrate, D-Neu5Ac.

174 2-keto-3-deoxy-6-phosphogalactonate (KDPGal) aldolase for microbial synthesis of shikimate pathway pr

175 ribed here uses recombinant His6-tagged KDPG aldolase for the synthesis of (S)-4-hydroxy-2-keto-4-(2'

176 light the chemical versatility of artificial aldolases for the practical synthesis of important chira

177 Previous studies have shown that aldolase forms a critical bridge between actin filaments

178 that photosynthetic eukaryotes acquired KDPG aldolase from the cyanobacterial ancestors of plastids v

179 Active KDPG aldolases from the cyanobacterium Synechocystis and the

180 pecific aldol addition catalyzed by pyruvate aldolases from the Entner-Doudoroff and the DeLey-Doudor

181 The family includes the "classical" aldolases fructose-1,6-(bis)phosphate (FBP) aldolase, tr

182 the recently discovered fructose-6-phosphate aldolase (FSA), which is functionally distinct from know

183 spacer region, and the fructose biphosphate aldolase gene.

184 protein (IbpA) and a putative allo-threonine aldolase (GlyI).

185 This class II aldolase has an active site zinc and a non-catalytic zin

186 A, its function in the catalytic reaction of aldolase has not been demonstrated.

187 Aldolases have emerged as key enzymes involved in these

188 n aldolase B with the A149P substitution (AP-aldolase) have shown that the mutation leads to losses i

189 ved from a class I tagatose-1,6-bisphosphate aldolase homologous to those involved in lactose and gal

190 uctures of divalent metal-dependent pyruvate aldolase, HpaI, in complex with substrate and cleavage p

191 putative 4-hydroxy-2-ketoheptane-1,7-dioate aldolase (HpcH) in the sequence databases.

192 alyzed by 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (HpcH), a member of the divalent metal ion depe

193 relative to that of wild-type E. coli KDPGal aldolase in catalyzing the addition of pyruvate to d-ery

194 proteins, some of which indeed interact with aldolase in pull-down assays, suggest supplementary, non

195 me reaction as that catalyzed by Eda, a KDGP aldolase in the Entner-Doudoroff pathway, and the two en

196 membrane protein 1 (TgAMA1), which binds to aldolase in vitro.

197 ), which is functionally distinct from known aldolases in its tolerance of different donor substrates

198 ic properties and tetrameric organization of aldolases in vitro.

199 nt absence of a key enzyme, dihydroneopterin aldolase, in the classical folate biosynthetic pathway o

200 Second, biochemical studies showed that aldolase indeed catalyzed these reactions.

201 ose of the actin-nucleating factor WASP with aldolase, indicating considerable plasticity in mechanis

202 Both neuronal aldolases interact specifically with the NF-L but not th

203 The TRAP-aldolase interaction is a distinctive and critical trait

204 5, alpha(v), and beta1 integrin subunits and aldolase (internal control).

205 n mechanism associated with metallodependent aldolases involving recruitment of the catalytic zinc io

206 The aldehyde intermediate produced by the aldolase is channeled directly through a buried molecula

207 in the case of TRAP, the binding of WASp to aldolase is competitively inhibited by the enzyme substr

208 Accordingly, SNX9 binding to aldolase is structurally precluded by the binding of sub

209 eda gene, which encodes the Entner-Doudoroff aldolase, is central to the catabolism of several sugar

210 , 2-keto-3-deoxygluconate-6-phosphate (KDPG) aldolase, is widespread in cyanobacteria, moss, fern, al

211 ciated with the variable activities of human aldolase isoenzymes modulated LacD.1's affinity for subs

212 are chloroplastic fructose 1,6-bisphosphate aldolase isoforms.

213 provides the first evidence for three novel aldolase isozymes in mouse sperm, two encoded by Aldoart

214 among fructose-1,6-(bis)phosphate aldolase (aldolase) isozymes.

215 Consistent with this hypothesis, aldolase knockdown cells show increased multinucleation.

216 However, aldolase knockdown does not affect glycolytic flux or in

217 One possible model for how aldolase knockdown may inhibit transformed cell prolifer

218 tococcus pyogenes, the tagatose bisphosphate aldolase LacD.1 likely originated through a gene duplica

219 Although a streptococcal aldolase, LacD.1, has been adapted to virulence gene reg

220 inding and photoactivation of labeled GAPDH, aldolase, lactate dehydrogenase, and pyruvate kinase rev

221 juvenile DM, creatinine phosphokinase level, aldolase level, absolute number of CD3-CD56+/16+ natural

222 as normal, as were serum creatine kinase and aldolase levels and thyroid, hepatic, and renal function

223 showed a significant correlation with serum aldolase levels.

224 gests YfaU is instead a 2-keto-3-deoxy sugar aldolase like the homologous 2-dehydro-3-deoxygalactarat

225 ysis of such decarboxylations proceeds by an aldolase-like mechanism.

226 isomerase (MtnA), and an annotated class II aldolase-like protein (Ald2) to form 2-(methylthio)aceta

227 d5RP aldolase (TgDERA), and a divergent dR5P aldolase-like protein (TgDPA) exclusively in the latent

228 ochemical analyses of T. gondii aldolase and aldolase-like proteins reveal diverse functionalization

229 catabolism is initiated by an intracellular aldolase/lyase mechanism.

230 nce of muscle edema in scleroderma, and that aldolase may be a useful biomarker to predict incident m

231 f cancer cell proliferation and suggest that aldolase may be a useful target in the treatment of canc

232 We have reported that the glycolytic enzyme aldolase mediates V-ATPase assembly and activity by phys

233 in the WASp family members and suggest that aldolase modulates the motility and actin dynamics of ma

234 in folate biosynthesis, 7,8-dihydroneopterin aldolase (Mt-FolB), have C-terminal tails that could als

235 M.tuberculosis 7,8-dihydroneopterin aldolase (Mtb FolB, DHNA) is the second enzyme in the fo

236 that is unique to bacteria, dihydroneopterin aldolase (MtDHNA).

237 lucose, whereas the catalysis-defective D34S aldolase mutant, which still binds FBP, blocks AMPK acti

238 tive Thr deaminase in both tha1 and tha2 Thr aldolase mutants greatly increases seed Ile content, sug

239 Functional analysis of the aldolase mutants shows that disruption of binding betwee

240 In this study, we generate aldolase mutants that lack binding to the B subunit of V

241 by the combined action of transketolase and aldolase, of the seven-carbon bisphosphorylated sugar se

242 ase (PdxA) oxidative decarboxylase, class II aldolase, or ribulose 1,5-bisphosphate carboxylase/oxyge

243 sphate and CO2 and (ii) the DUF1537/class II aldolase pair participates in pathways for the conversio

244 2.4-A and 2.7-A structures of P. falciparum aldolase (PfAldo) obtained from crystals grown in the pr

245 nal unidirectional fructose 1,6-bisphosphate aldolase/phosphatase, have been identified.

246 ood was fixed and stained with Abs to GAPDH, aldolase, phosphofructokinase (PFK), pyruvate kinase (PK

247 to glyceraldehyde-3-phosphate dehydrogenase, aldolase, phosphofructokinase, lactate dehydrogenase, an

248 omesin 3 and filamin-C), glycolytic enzymes (aldolase, phosphoglycerate mutase 2, beta enolase and gl

249 A sialate aldolase (pm1715) mutant unable to initiate dissimilatio

250 itions 51% in the first turnover to form the aldolase products, 24% to the epimerase product and 25%

251 When unoccupied by FBP, aldolases promote the formation of a lysosomal complex c

252 th kinetic and structural studies on natural aldolases provides valuable feedback for computational e

253 Human V-ATPase directly interacts with aldolase, providing a coupling mechanism for glucose met

254 tory of the computationally designed (retro-)aldolase RA95.

255 Here we show that the artificial retro-aldolase RA95.5-8 is able to use a reactive lysine in a

256 The artificial aldolase RA95.5-8, for example, exploits amine catalysis

257 The aldolase reaction is strongly pH dependent, and apparent

258 rmediates (which would be generated from the aldolase reaction on each of these substrates) to move t

259 The aldolase reaction yields pyruvate, which supports growth

260 thermore, chemistry is rate limiting for the aldolase reaction, and the analysis of solvent kinetic i

261 ydroxyacetone phosphate and erythrose via an aldolase reaction.

262 The protein sequence of the evolved aldolase showed eight amino acid changes from the native

263 Yeast GAPDH and aldolase showed low-affinity binding to yeast actin, whi

264 sight into the molecular determinants of FBP aldolase stereospecificity during aldol addition, a key

265 The AP-aldolase structure reveals the molecular basis of a here

266 pFG is a bifunctional enzyme comprised of an aldolase subunit, DmpG, and a dehydrogenase subunit, Dmp

267 is classified as a new member of the class I aldolase superfamily.

268 ed enzyme, E. coli tagatose-1,6-bisphosphate aldolase (TBPA), are described.

269 Moreover, native top-down ECD of aldolase tetramer reveals that ECD fragmentation is not

270 fitting of the isotopic distribution of the aldolase tetramer.

271 ssful apicomplexan parasite, expresses F16BP aldolase (TgALD1), d5RP aldolase (TgDERA), and a diverge

272 ite, expresses F16BP aldolase (TgALD1), d5RP aldolase (TgDERA), and a divergent dR5P aldolase-like pr

273 Whereas one Arabidopsis thaliana Thr aldolase (THA1) is expressed primarily in seeds and seed

274 yase (NAL, E.C. number 4.1.3.3) is a Class I aldolase that catalyzes the reversible aldol cleavage of

275 ne of the operon is nanL, which codes for an aldolase that cleaves NANA into N-acetyl mannosamine (ma

276 s for multistep reactions, we designed retro-aldolases that use four different catalytic motifs to ca

277 fuculose-1-phosphate by fuculose-1-phosphate aldolase, the MJ1418 gene product.

278 of calpain, Sm29, and fructose-bisphosphate aldolase, themselves potential vaccine antigens, suggest

279 s its cytoplasmic tail connects to actin via aldolase, thus driving parasite motility and host cell i

280 ar channel in the protein structure from the aldolase to the dehydrogenase active site.

281 ivity assays on the surfaces with DNA-linked aldolase to validate that, despite being modified with D

282 aldolases fructose-1,6-(bis)phosphate (FBP) aldolase, transaldolase, and 2-keto-3-deoxy-6-phosphoglu

283 e-site-directed mutagenesis to afford KDPGal aldolase variant NR8.276-2, which exhibits a 60-fold imp

284 could be rescued with an enzymatically dead aldolase variant that retains the known F-actin binding

285 on, where SNX9 binds near the active site of aldolase via residues 165-171 that are also required for

286 vitro and in vivo binding of WASp members to aldolase was characterized by biochemical, deletion mapp

287 t L-3-deoxy-manno-2-octulosonic acid (L-KDO) aldolase was created by directed evolution from the Esch

288 E. coli KDPGal aldolase was evolved using a combination of error-prone

289 ght into the function of different T. gondii aldolases, we first determined the crystal structures of

290 SFEC, pyruvate kinase and aldolase were co-localized by immunofluorescence to the

291 In cooked or roasted foods, enolase and aldolase were detectable in chicken breast while parvalb

292 isphosphatase, and fructose-1,6-bisphosphate aldolase were indicated.

293 minimal requirements for the TRAP binding to aldolase were scanned here and found to be shared by dif

294 an be used as a functional mimic of tagatose aldolase, whereas (R)-proline can be regarded as an orga

295 is required for the direct interaction with aldolase, whereas the second upstream acidic region is n

296 ractions with the abundant glycolytic enzyme aldolase, which also binds to the LC4 domain of SNX9.

297 ral metabolic function resides in the LacD.2 aldolase, which is required for the catabolism of galact

298 rease in glycolysis at the step catalyzed by aldolase, while activating PIK3CA mutations have the opp

299 ion of a 158 kDa protein complex, tetrameric aldolase with an average absolute deviation of 0.36 ppm

300 th AtLSMT-L and PsLSMT are able to methylate aldolases with similar kinetic parameters and product sp