ライフサイエンスコーパス: aleurone

1 lower expression in wild type relative to d1 aleurone.

2 egulate conversion of oil to sugar in barley aleurone.

3 roduction of very long chain omega-7s in the aleurone.

4 utant plants did not show a phenotype in the aleurone.

5 appearance of alpha-amylase released by the aleurone.

6 tivator of gene expression in the developing aleurone.

7 inhibitor of gibberellin signaling in barley aleurone.

8 nin pathway exhibits strict cell autonomy in aleurone.

9 minished the expression of the Amy1A gene in aleurone.

10 asts of barley (Hordeum vulgare cv Himalaya) aleurone.

11 rall increase in flavonoid production in the aleurone.

12 subcellular localization of ferulate in the aleurone.

13 al estimated from the total content of FA in aleurone.

14 e carotenoids and zeins and to differentiate aleurone.

15 ors showed localized patches of multilayered aleurone.

16 of these proteins also occurred in isolated aleurones after treatment with gibberellin, and this was

17 ence 6.4% of the transcriptome in developing aleurone and 6.7% in starchy endosperm.

18 eak alleles result in endosperms with mosaic aleurone and deformed plants with epidermal cells that r

19 rane preparations from oat (Avena sativa L.) aleurone and from leaves and stems of wild-type and GA-s

20 ctor ABI5, as assayed functionally in barley aleurone and physically in the yeast-two-hybrid assay.

21 High levels are also observed in the aleurone and scutellum after germination, while low leve

22 cus, required for anthocyanin pigment in the aleurone and scutellum of the Zea mays (maize) seed, was

23 We show that both aleurone and starchy endosperm cells can be successfully

24 In contrast to aleurone and starchy endosperm cells, transfer cells fai

25 The aleurone and starchy endosperm share a common lineage th

26 ultured endosperms undergo normal cell type (aleurone and starchy endosperm) differentiation and stor

27 s system has been evaluated by analysing the aleurone and sub-aleurone cells of mature wheat grain, s

28 RNase) expressed in barley (Hordeum vulgare) aleurone and the gene for a second barley RNase expresse

29 with C1 for anthocyanin pigmentation in the aleurone and wx1 for amylose synthesis in the starchy en

30 lso contributes to omega-7 production in the aleurone, and aad3 aad2 exhibits an approximately 85% re

31 ad higher expression in wild type than in d1 aleurone, and genes down-regulated by GA had lower expre

32 tion of OsCBL2 and HvCBL2 in rice and barley aleurone because changes in cytosolic calcium have been

33 responding mRNA levels in the mature imbibed aleurone but are repressed 10-fold or more within 24 h o

34 enhanced the radical scavenging activity of aleurone by up to 4-fold, which overlapped the overall a

35 to horseradish peroxidase to screen a barley aleurone cDNA expression library for CaM binding protein

36 ase 1 and protein phosphatase 2A from a rice aleurone cDNA library.

37 , grain and grain cells (transfer cell (TC), aleurone cell (AL), and starchy endosperm (SE)).

38 bscisic acid (ABA) slows down the process of aleurone cell death and isolated aleurone protoplasts ca

39 Aleurone cell death does not follow the apoptotic pathwa

40 altered pattern of anthocyanin synthesis and aleurone cell differentiation in endosperm.

41 sts a hierarchy of gene functions to specify aleurone cell fate and then control aleurone differentia

42 onally, our data suggest that acquisition of aleurone cell fate does not solely rely upon signalling

43 URONE LAYER1 (SAL1), a negative regulator of aleurone cell fate encoding a class E vacuolar sorting p

44 ntrols a gene regulatory network involved in aleurone cell fate specification and cell differentiatio

45 These data suggest a model for aleurone cell fate specification in which DEK1 perceives

46 -specific fluorescence markers, we show that aleurone cell fate specification occurs exclusively in r

47 NKLY4 (CR4), a receptor kinase implicated in aleurone cell fate specification, colocalized to plasma

48 k1) gene is a central regulator required for aleurone cell fate specification.

49 The starchy endosperm and aleurone cell fates are freely interchangeable throughou

50 teinase region (DEK1-CALP), is essential for aleurone cell formation at the surface of maize (Zea may

51 the role of vacuole acidification in cereal aleurone cell function.

52 The relationship between the aleurone cell integrity and the exposure or release of b

53 Vacuolation of the aleurone cell is a diagnostic feature of the response to

54 ndosperm transfer cell layer (BETL), and the aleurone cell layer (AL).

55 number of mRNA species in the mature imbibed aleurone cell of barley, such as a storage globulin, are

56 nd OsCBL3 were localized to the tonoplast of aleurone cell protein storage vacuoles and OsCBL4-green

57 have been implicated in the response of the aleurone cell to GA.

58 required for the ABA response of the cereal aleurone cell.

59 iggering the subsequent ABA responses of the aleurone cell.

60 Unlike the starchy endosperm, aleurone cells accumulate these storage proteins inside

61 Sporadic aleurone cells along the fusion plane were observed and

62 epressor, OsWRKY71 is localized to nuclei of aleurone cells and binds specifically to functionally de

63 s, with no difference being detected between aleurone cells and starchy endosperm cells.

64 Cereal aleurone cells are specialized endosperm cells that func

65 vacuole (PSV) becomes acidified rapidly when aleurone cells are treated with gibberellic acid (GA) bu

66 Unlike the cells of the starchy endosperm, aleurone cells are viable in mature grain but undergo PC

67 Mutants possess multiple layers of aleurone cells as well as aborted embryos.

68 Over-expression of HvWRKY38 in aleurone cells by particle bombardment blocks GA inducti

69 Barley aleurone cells contain three nucleases whose activities

70 lifespan of the endosperm, with internalized aleurone cells converting to starchy endosperm cells and

71 mation of apoptotic bodies do not occur when aleurone cells die.

72 p1-mediated repression of hydrolase genes in aleurone cells during seed development is determined by

73 of fluorescent dye tracers showed that young aleurone cells established symplastic subdomains through

74 or OsCBL2 in promoting vacuolation of barley aleurone cells following treatment with GA.

75 ) mutant line carrying up to seven layers of aleurone cells in defective kernel endosperm compared wi

76 sal1-2 endosperm has two to three layers of aleurone cells in normal, well filled grains.

77 The opening of aleurone cells increased the physical exposure of FA bou

78 PCD in barley aleurone cells is accompanied by the accumulation of a s

79 The expression of HvWRKY38 in barley aleurone cells is down-regulated by GA, but up-regulated

80 We conclude that cell death in aleurone cells is hormonally regulated and is the final

81 that GA-induced alpha-amylase production in aleurone cells is inhibited by bioactive SA, but not its

82 We tested the hypothesis that PCD in aleurone cells occurs by apoptosis, and show that the ha

83 d by these hormones were investigated in the aleurone cells of barley seeds using double-stranded RNA

84 The Vp1 promoter is active in the embryo and aleurone cells of developing seeds and, upon drought str

85 Constitutive expression of TaABF1 in aleurone cells of imbibing grains completely eliminated

86 evaluated by analysing the aleurone and sub-aleurone cells of mature wheat grain, showing high spati

87 In the aleurone cells of the cereal grain, gibberellic acid (GA

88 enzymes (principally alpha-amylases) in the aleurone cells of the endosperm, which then mobilize the

89 bombardment-mediated transient expression in aleurone cells represses the expression of two reporter

90 Arabidopsis aleurone cells responded to nitric oxide (NO), gibberell

91 Sensitivity to H2O2 in GA-treated aleurone cells results from a decreased ability to metab

92 Gain- and loss-of-function studies in barley aleurone cells show that HvABI5 expression is positively

93 Over-expression of these two genes in aleurone cells specifically and synergistically represse

94 y-GUS reporter gene in developing vp1 mutant aleurone cells strongly depends on the presence of a viv

95 signal transduction pathways exist in cereal aleurone cells that enable them to modulate hydrolase pr

96 ents occurring until late development caused aleurone cells to switch fate to starchy endosperm indic

97 The percentage of aleurone cells transformed following this method varied

98 ll death was studied in barley (cv Himalaya) aleurone cells treated with abscisic acid and gibberelli

99 ive and do not degrade their DNA, but living aleurone cells treated with GA accumulate nucleases and

100 Herein, we report transcriptomes of aleurone cells treated with the hormones abscisic acid,

101 Barley aleurone cells undergo programmed cell death (PCD) when

102 m cells remain competent to differentiate as aleurone cells until late in development.

103 1 and OsWRKY51 are revealed in the nuclei of aleurone cells using bimolecular fluorescence complement

104 ta-glucuronidase, and introduced into barley aleurone cells using the particle bombardment method.

105 ase reporter genes when introduced to barley aleurone cells via particle bombardment.

106 Arabidopsis aleurone cells were examined by light and electron micro

107 ination of the connated kernel revealed that aleurone cells were present for only a short distance al

108 d) rather than cyanidin (purple) pigments in aleurone cells where the anthocyanin biosynthetic pathwa

109 ave pleiotropic phenotypes including lack of aleurone cells, aborted embryos, carotenoid deficiency,

110 nsists of an epidermal-like surface layer of aleurone cells, an underlying body of starchy endosperm

111 ment of aleurone signaling molecules between aleurone cells, and SAL1 maintains the proper plasma mem

112 ansduction pathway were introduced into rice aleurone cells, and then the level of the OsCa-atpase tr

113 In a transient assay in rice aleurone cells, expression of the introduced Ca(2+)-ATPa

114 in regulating GAMYB-mediated GA signaling in aleurone cells, thereby establishing a novel mechanism f

115 y the components of transduction pathways in aleurone cells, we have investigated the effect of okada

116 transcriptional repressor of GA signaling in aleurone cells.

117 nd -72 are induced by abscisic acid (ABA) in aleurone cells.

118 d-induced HVA22 or HVA1 promoter activity in aleurone cells.

119 among which OsWRKY71 is highly expressed in aleurone cells.

120 utant allele contains two to three layers of aleurone cells.

121 apoptotic bodies, are not observed in dying aleurone cells.

122 s in death of GA-treated but not ABA-treated aleurone cells.

123 ROS in hormonally regulated death of barley aleurone cells.

124 nally regulated cell death pathway in barley aleurone cells.

125 of nuclease activities correlate with PCD in aleurone cells.

126 ed for the GA-dependent signaling pathway in aleurone cells.

127 lin (GA)-dependent signaling pathway in rice aleurone cells.

128 ot only in the starchy endosperm but also in aleurone cells.

129 oter regulate its expression level in barley aleurone cells.

130 ultrastructure was similar to that of cereal aleurone cells.

131 tially associated with plasmodesmata between aleurone cells.

132 come positioned at the surface converting to aleurone cells.

133 -inducible and GA-repressible in embryos and aleurone cells.

134 The pale aleurone color1 (pac1) locus, required for anthocyanin p

135 However, aad3 ssi2 aleurone contained the same amount of omega-7s as aad3 W

136 mainder of the fusion plane, suggesting that aleurone development is suppressed when positioned betwe

137 Excision of the Sho element early in aleurone development results in the characteristic "marb

138 A genetic analysis of maize aleurone development was conducted.

139 s, suggesting that cytokinin can also affect aleurone development.

140 as the testa and pericarp are important for aleurone development.

141 These mutants disrupt aleurone differentiation in reproducible patterns sugges

142 analysis of additional mutants that disrupt aleurone differentiation suggests a hierarchy of gene fu

143 specify aleurone cell fate and then control aleurone differentiation.

144 novel negative function in the regulation of aleurone differentiation.

145 ymes used for protoplast preparation degrade aleurone DNA and that DNA degradation by these nucleases

146 To analyze signaling between the embryo and aleurone during seed development, a T-B3La chromosome tr

147 stream sequence conferred the same levels of aleurone expression as nontransgenic B-Peru plants, but

148 al cues are required to specify and maintain aleurone fate and Dek1 function is required to respond t

149 ment indicating that positional cues specify aleurone fate.

150 Mutants in dek1 block aleurone formation at an early stage and cause periphera

151 In the seed, loss of cr4 inhibits aleurone formation in a pattern that reflects the normal

152 were hand dissected into endosperm, germ and aleurone fractions.

153 The cereal aleurone functions during germination by secreting hydro

154 iated cells that show sporadic expression of aleurone identity markers such as a viviparous1 promoter

155 c acid (FA) with the antioxidant capacity of aleurone in in vitro and under simulated gastric conditi

156 nucleus of both protonemal cells and barley aleurone, indicating that the nuclear localization signa

157 ng programmed cell death (PCD) in the cereal aleurone is described.

158 cPrG acts as a potential H+/Cl- symporter in aleurone is presented.

159 The aleurone is the outermost layer of cereal endosperm and

160 riched in palmitic acid, while the seed coat/aleurone layer accumulated vaccenic, linoleic, and alpha

161 present to different degrees in the maturing aleurone layer and embryo, but not in the starchy endosp

162 utermost cells, which differentiate into the aleurone layer and remain living in the mature seed.

163 measured, and these data show that both the aleurone layer and the embryo expressed the NO-associate

164 general and emphasize the versatility of the aleurone layer as a model system for studying plant prot

165 istically to regulate gluconeogenesis in the aleurone layer as well as controlling the production and

166 The aleurone layer had zeaxanthin levels 2- to 5-fold higher

167 The cereal aleurone layer is a model system for studying the regula

168 The barley aleurone layer is a terminally differentiated secretory

169 The maize (Zea mays) aleurone layer occupies the single outermost layer of th

170 ted close correlation between embryo and the aleurone layer of endosperm.

171 Development of the aleurone layer of maize grains requires the activity of

172 dlings, vascular tissue of nodes, embryo and aleurone layer of seeds, and young flowers.

173 b mRNA occurred in the starchy endosperm and aleurone layer of the developing seed, but not in the em

174 ost b alleles in that it is expressed in the aleurone layer of the seed.

175 ysis identified 73 glycosylation sites in 65 aleurone layer proteins, with 53 of the glycoproteins fo

176 er of starchy endosperm cells underneath the aleurone layer showed transformed cells.

177 The aleurone layer was found to be the primary determinant o

178 repressible mRNA persists in the mature dry aleurone layer, but is degraded during imbibition, reple

179 as normal maize (Zea mays [Zm]) has a single aleurone layer, naked endosperm (nkd) mutants produce mu

180 contrast, MT4 was confined to the embryo and aleurone layer, where it appeared during tissue speciali

181 Among those yet to be studied is the aleurone layer, which produces hydrolases for mobilizati

182 age oil is a major constituent in the cereal aleurone layer.

183 L gene, OsCBL2, is up-regulated by GA in the aleurone layer.

184 and in the amount of ions released from the aleurone layer.

185 sis and the endosperm is chalky and lacks an aleurone layer.

186 t to report on carotenoid composition of the aleurone layer.

187 ed; this gene and the gene encoding the seed aleurone-layer xylanase had strict tissue-specific expre

188 ces of many microbial xylanases and a barley aleurone-layer xylanase.

189 SUPERNUMERARY ALEURONE LAYER1 (SAL1), a negative regulator of aleurone

190 ransposon lines identified the supernumerary aleurone layers 1-1 (sal1-1) mutant line carrying up to

191 perm would affect the development of the two aleurone layers along the fusion plane.

192 the level of its mRNA readily detectable in aleurone layers and embryos, yet undetectable in the sta

193 show that cPrG prevents PSV acidification in aleurone layers and prevents synthesis of secretory prot

194 When barley (Hordeum vulgare) aleurone layers are subjected to heat shock there is a s

195 rotein secretion in gibberellic acid-induced aleurone layers by two independent mechanisms, heat shoc

196 of gene constructs were introduced to barley aleurone layers by using particle bombardment: the repor

197 Incubation of barley (Hordeum vulgare) aleurone layers in H2O2 causes rapid death of all cells

198 demonstrated that cycloheximide treatment of aleurone layers increased mRNA levels 4-fold, whereas a

199 Mutant kernels have several aleurone layers instead of one, indicating that Xcl1 alt

200 Barley (Hordeum vulgare) aleurone layers maintained in vitro respond to gibberell

201 eds lacking the testa, embryos, and isolated aleurone layers of Arabidopsis (Arabidopsis thaliana) we

202 suppression of genes has been determined in aleurone layers of barley seeds.

203 sion of genes, such as alpha-amylase, in the aleurone layers of cereals.

204 en germination is triggered or when isolated aleurone layers or protoplasts are incubated in gibberel

205 Incubation of aleurone layers or protoplasts in H(2)O(2)-containing me

206 revent DNA degradation during isolation from aleurone layers or protoplasts.

207 lecular changes that occurred in embryos and aleurone layers prior to germination were measured, and

208 fluorescent probe, Hordeum vulgare (barley) aleurone layers produce NO rapidly when nitrite is added

209 2 mRNA decline rapidly following exposure of aleurone layers to GA.

210 ide dismutase are strongly down-regulated in aleurone layers treated with GA.

211 RNA under anoxic conditions was decreased in aleurone layers while it increased in the embryo.

212 ugh cPrG blocks many GA-induced responses of aleurone layers, it does not affect early steps in GA si

213 In barley aleurone layers, the expression of genes encoding alpha-

214 d phytate is inhibited by cPrG in GA-treated aleurone layers.

215 ssion have been previously defined in barley aleurone layers.

216 on of GA-inducible gene expression in cereal aleurone layers.

217 at shock proteins, decreased in heat-shocked aleurone layers.

218 ntibody for detection and an antibody to the aleurone-localised 8S globulin as a control.

219 Aleurone mosaicism was observed in the crown region of n

220 f in planta endosperm, including fidelity of aleurone mutant phenotypes, temporal and spatial control

221 We show that endogenous barley aleurone nucleases and nucleases present in enzymes used

222 Cell death in barley aleurone occurs only after cells become highly vacuolate

223 (omega-7s) are specifically enriched in the aleurone of Arabidopsis (Arabidopsis thaliana) seeds.

224 ated in pericarp layer of purple but only in aleurone of blue corn.

225 p101 mRNA accumulated in the subaleurone and aleurone of developing kernels and was highest in the ro

226 We also measured transcript abundance in aleurone of dwarf1 (d1) mutant rice.

227 seeds at early stages and in the embryo and aleurone of germinating seeds up to 24 h of imbibition.

228 Synthesis of the recombinant enzyme in the aleurone of germinating transgenic grain with an alpha-a

229 ious induction of alpha-amylase genes in the aleurone of the developing seed.

230 articularly in the leaf epidermis and in the aleurone of the endosperm.

231 Our findings suggest that the aleurone of wheat, oat, corn and germ of barley have sig

232 nucleases play a role in DNA cleavage during aleurone PCD.

233 n B-Bolivia that can account for the reduced aleurone pigment amounts (40%) observed with B-Bolivia r

234 of R-sc, r-m9 conditions a reduced level of aleurone pigmentation due to the presence of a 2.1-kb Ds

235 ment results in the characteristic "marbled" aleurone pigmentation pattern conferred by R-mb.

236 it to PLD activation are associated with the aleurone plasma membrane.

237 In barley aleurone, PpABI3A transactivates Em-GUS but to a lesser

238 ive oxygen species (ROS) are key elements in aleurone programmed cell death.

239 ther, these data demonstrate that GA-treated aleurone protoplasts are less able than ABA-treated prot

240 process of aleurone cell death and isolated aleurone protoplasts can be kept alive in media containi

241 Light microscopy showed that aleurone protoplasts contain two distinct types of vacuo

242 We report that the application of ABA to aleurone protoplasts increased the activity of the enzym

243 Aleurone protoplasts incubated in abscisic acid remained

244 at the pH of secondary vacuoles was lower in aleurone protoplasts incubated in gibberellic acid than

245 Application of pertussis toxin to intact aleurone protoplasts inhibited the ability of ABA to act

246 The application of PPA to aleurone protoplasts led to an ABA-like inhibition of al

247 nducible or otherwise was detected in barley aleurone protoplasts transfected with the PpLEA-1::GUS c

248 Illumination of barley aleurone protoplasts with blue or UV-A light results in

249 fluorescent protein was expressed in barley aleurone protoplasts, fluorescence accumulated in the pl

250 vitro in microsomal membranes prepared from aleurone protoplasts.

251 Aleurone PSVs contain zein-rich protein inclusions, a ma

252 embled in the ER, zeins are delivered to the aleurone PSVs in atypical prevacuolar compartments that

253 er wheat, spring tritordeum and barley (blue aleurone, purple pericarp, and yellow endosperm) from th

254 min with 0-20 L/h steam) were applied on the aleurone-rich flour to modify the technological properti

255 Novel aleurone-rich wheat milling fraction developed and produ

256 one regulation of expression of mRNA for the aleurone RNase revealed that, like the pattern for alpha

257 g Sc+ expression, including darkly pigmented aleurone, scutellum, coleoptile, and scutellar node [Scp

258 The role of DEK1 in aleurone signaling is discussed.

259 signal, CR4 promotes the lateral movement of aleurone signaling molecules between aleurone cells, and

260 e roles for the function of the sal1 gene in aleurone signaling, including a defect in endosome traff

261 One uses the promoters of genes with aleurone-specific expression during germination and the

262 Immediately upstream of the aleurone-specific promoter elements and in the opposite

263 Our findings that novel aleurone-specific promoter sequences of the B-Peru trans

264 ose through the translocation of an existing aleurone-specific promoter to the b locus.

265 tion assays in aleurone tissue localized the aleurone-specific promoter to the first 176 bases of the

266 f dek1 in the signaling system that controls aleurone specification and other aspects of endosperm de

267 n between a viviparous vp1 embryo and mutant aleurone suggesting that a quiescent embyro is a source

268 lar enhancement of Amy-GUS expression in the aleurone, suggesting that the quiescent embryo present i

269 In isolated aleurones, the levels of the corresponding mRNA increase

270 Transient transformation assays in aleurone tissue localized the aleurone-specific promoter

271 ium within 24 h of seed germination, but the aleurone tissue surrounding the starchy endosperm eventu

272 a-glucosidase cDNA clone derived from barley aleurone tissue was expressed in Pichia pastoris and Esc

273 In cereal aleurone tissue, GA induces and ABA suppresses the expre

274 ast development, alpha-amylase production in aleurone tissue, NO-dependent expression of defence-rela

275 anin pathway but is expressed exclusively in aleurone tissue.

276 starch spreads across the endosperm from the aleurone towards the crease.

277 Using a barley aleurone transient expression system, we have now locali

278 ption factor, VP1, on ABA action in a barley aleurone transient expression system.

279 imately 11,000 hybridized with RNA from rice aleurone treated with ABA, GA, or no hormone.

280 cDNA library constructed from mRNA of barley aleurones treated with gibberellin A 3 (GA).

281 lpha-amylase genes are expressed in wild oat aleurone, two genes, alpha-Amy2/A and alpha-Amy2/D, were

282 rized gene expression in rice (Oryza sativa) aleurone using a half-genome rice microarray.

283 d the V-type H(+)-ATPase present in isolated aleurone vacuoles.

284 The pattern of aleurone variegation of maize kernels carrying Ac and bz

285 alls of the starchy endosperm but not in the aleurone walls.

286 e improvement in the antioxidant capacity of aleurone was also observed in the simulated gastric dige

287 The antioxidant capacity of aleurone was increased by around 2-fold when its median

288 oligosaccharides (AXOS) obtained from wheat aleurone was investigated.

289 und that AAD3 expression is localized to the aleurone where mutants show an approximately 50% reducti

290 a+ and K+ within the phytate granules of the aleurone, with CN- being diagnostic for proteins and C(2

291 ied in the omega-7 content of Brassica napus aleurone, with the highest level detected being approxim