ライフサイエンスコーパス: alkyltransferase

1 by a suicide protein, O(6)-alkylguanine-DNA alkyltransferase.

2 n the DNA repair protein O6-alkylguanine-DNA alkyltransferase.

3 ay using monoclonal antibodies against human alkyltransferase.

4 tested for their ability to inactivate human alkyltransferase.

5 hways devoted to their repair, including DNA alkyltransferases.

6 ces the cysteine at the known active site of alkyltransferases.

7 an CblC exhibits glutathione (GSH)-dependent alkyltransferase activity and flavin-dependent reductive

8 In addition, O6-benzylguanine depleted alkyltransferase activity in BM cells at concentrations

9 These mutations had little effect on the alkyltransferase activity in repairing O6-methylguanine

10 This alkyltransferase activity is apparently catalyzed at a f

11 e, despite lacking the reactive cysteine and alkyltransferase activity of AGT.

12 xenografts displayed no O6-alkylguanine-DNA alkyltransferase activity, and their levels of glutathio

13 rtional to inhibition of O6-alkylguanine-DNA alkyltransferase activity, but a maximum tolerated dose

14 nitors were also found to have low levels of alkyltransferase activity.

15 nal enzyme with both glycoside hydrolase and alkyltransferase activity.

16 However, the Escherichia coli alkyltransferase, Ada-C, is not inactivated by O6-benzyl

17 r ovary (CHO) cells lack O6-alkylguanine-DNA alkyltransferase (AGT) activity and are sensitive to kil

18 rrow showed BG-resistant O6-alkylguanine-DNA-alkyltransferase (AGT) activity, and CFUs were stained i

19 f the DNA repair protein O6-alkylguanine-DNA alkyltransferase (AGT) and are sensitive to 1,3-bis(2-ch

20 f the DNA repair protein O6-alkylguanine-DNA-alkyltransferase (AGT) and increases rates of both spont

21 er chemotherapy target O(6)-alkylguanine-DNA alkyltransferase (AGT) and paradoxically protect cells f

22 h it is known that (i) O(6)-alkylguanine-DNA alkyltransferase (AGT) confers tumor cell resistance to

23 MT), also referred to as O6-alkylguanine-DNA alkyltransferase (AGT) correlate with the resistance of

24 the DNA repair enzyme O(6)-alkylguanine DNA alkyltransferase (AGT) from the O(6)-methylguanine methy

25 The DNA repair protein O6-alkylguanine-DNA alkyltransferase (AGT) has been shown to protect cells f

26 To evaluate the role of O6-alkylguanine-DNA alkyltransferase (AGT) in colon tumor chloroethylnitroso

27 O(6)-Alkylguanine-DNA alkyltransferase (AGT) is a DNA repair protein which rem

28 O6-alkylguanine-DNA alkyltransferase (AGT) is a DNA-repair protein that reve

29 O6-alkylguanine DNA alkyltransferase (AGT) is a key mechanism in the prevent

30 O(6)-alkylguanine-DNA alkyltransferase (AGT) is a single-cycle DNA repair enzy

31 O6-Alkylguanine-DNA alkyltransferase (AGT) is a suicide enzyme that repairs

32 O(6)-alkylguanine-DNA alkyltransferase (AGT) is a ubiquitous enzyme with an am

33 O6-Alkylguanine-DNA alkyltransferase (AGT) is an important cellular defense

34 O6-Alkylguanine-DNA alkyltransferase (AGT) is an important DNA repair protei

35 O6-Alklyguanine-DNA alkyltransferase (AGT) is an important DNA repair protei

36 the DNA repair protein O(6)-alkylguanine-DNA alkyltransferase (AGT) is an important source of tumor c

37 The DNA repair protein O(6)-alkylguanine alkyltransferase (AGT) is responsible for removing promu

38 infusion that suppresses O6-alkylguanine-DNA alkyltransferase (AGT) levels in brain tumors, (2) evalu

39 Another way to deplete O6-alkylguanine DNA alkyltransferase (AGT) levels is to modify methylating a

40 Seventeen O(6)-alkylguanine-DNA alkyltransferase (AGT) mutants highly resistant to O(6)-

41 the DNA repair protein O(6)-alkylguanine-DNA alkyltransferase (AGT) paradoxically increases the mutag

42 O(6)-Alkylguanine-DNA alkyltransferase (AGT) plays a critical role in protecti

43 O(6)-Alkylguanine-DNA alkyltransferase (AGT) plays a major role in repair of t

44 O (6)-Alkylguanine-DNA alkyltransferase (AGT) plays an important role by protec

45 The DNA repair protein O6-alkylguanine-DNA alkyltransferase (AGT) protects cells from alkylation da

46 The activity of O6-alkylguanine-DNA alkyltransferase (AGT) protects cells from killing by me

47 The repair protein O(6)-alkylguanine-DNA alkyltransferase (AGT) protects cells from the mutagenic

48 Recombinant human O(6)-alkylguanine DNA alkyltransferase (AGT) protein or its variants (C145A an

49 O(6)-alkylguanine-DNA alkyltransferase (AGT) repairs damage to the human genom

50 O6-Alkylguanine-DNA alkyltransferase (AGT) repairs DNA by transferring the m

51 Human O6-alkylguanine-DNA alkyltransferase (AGT) repairs mutagenic O6-alkylguanine

52 The O6-alkylguanine-DNA alkyltransferase (AGT) repairs O6-alkylguanine and O4-al

53 ctive mutants of human O(6)-alkylguanine DNA alkyltransferase (AGT) show that it forms an O(6)-methyl

54 O6-alkylguanine-DNA alkyltransferase (AGT) specifically removes the methyl g

55 ity of O(6)-benzylguanine (BG) to inactivate alkyltransferase (AGT) to potentiate the antitumor effic

56 ligonucleotides by human O6-alkylguanine DNA alkyltransferase (AGT) were estimated using rapid reacti

57 active site domain of O(6)-alkylguanine-DNA alkyltransferase (AGT) with an endonuclease V domain.

58 uman DNA repair protein, O6-alkylguanine-DNA alkyltransferase (AGT), by O6-benzylguanine renders tumo

59 The DNA repair enzyme O(6)-alkylguanine DNA alkyltransferase (AGT), encoded by the gene MGMT, repair

60 O6alkylguanine-DNA alkyltransferase (AGT), encoded by the methylguanine met

61 the DNA repair protein, O6-alkylguanine-DNA alkyltransferase (AGT), has been shown to reduce nitroso

62 The DNA repair protein, O6-alkylguanine-DNA alkyltransferase (AGT), protects cells from the toxic an

63 the resistance protein O(6)-alkylguanine-DNA alkyltransferase (AGT), were synthesized and evaluated f

64 ical regulation of human O6-alkylguanine-DNA alkyltransferase (AGT), which determines the susceptibil

65 Human O(6)-alkylguanine-DNA alkyltransferase (AGT), which directly reverses endogeno

66 s the DNA repair protein O6-alkylguanine-DNA alkyltransferase (AGT), which removes chlorethylation or

67 the DNA repair protein O(6)-alkylguanine-DNA alkyltransferase (AGT), which removes chloroethylation o

68 istance to expression of O6-alkylguanine-DNA alkyltransferase (AGT), which repairs DNA damage caused

69 ells containing active O(6)-alkylguanine-DNA alkyltransferase (AGT), which repairs O(6)-meG, 3% misin

70 amines are repaired by O(6)-alkylguanine DNA alkyltransferase (AGT), which transfers the O(6)-alkyl g

71 e directly repaired by O(6)-alkylguanine-DNA alkyltransferase (AGT), which transfers the pyridyloxobu

72 itive factor (NSF) and O(6)-alklyguanine-DNA alkyltransferase (AGT), with moderate affinity (K approx

73 involvement of BRCA2 in O6-alkylguanine DNA alkyltransferase (AGT)-mediated repair of O6-methylguani

74 cy end point for overcoming alkylguanine DNA alkyltransferase (AGT)-mediated tumor cell resistance to

75 e repair of O(6)-mG by O(6)-alkylguanine-DNA alkyltransferase (AGT).

76 the DNA-repair protein O(6)-alkylguanine-DNA alkyltransferase (AGT).

77 n all known sequences of O6-alkylguanine-DNA alkyltransferase (AGT).

78 atch repair activity and O6-alkylguanine-DNA alkyltransferase (AGT).

79 BCNU resistance protein, O6-alkylguanine DNA alkyltransferase (AGT).

80 agents are reduced by O(6)-alkylguanine-DNA alkyltransferase (AGT).

81 s demethylation by the O(6)-alkylguanine-DNA alkyltransferase (AGT).

82 ated by the actions of O(6)-alkylguanine-DNA alkyltransferase (AGT).

83 rosslinks of DNA with O(6) -alkylguanine-DNA alkyltransferase (AGT, see picture).

84 ir proteins related to O(6)-alkylguanine-DNA alkyltransferases (AGTs) that tightly bind alkylated DNA

85 human DNA repair protein O6-alkylguanine-DNA alkyltransferase (alkyltransferase) in vitro than O6-ben

86 O6-Alkylguanine-DNA alkyltransferase (alkyltransferase) provides an importan

87 ive as inactivators of O(6)-alkylguanine-DNA alkyltransferase (alkyltransferase) than either O(6)-ben

88 ctural conformations related to the distinct alkyltransferase and acetyltransferase reactions catalyz

89 than O6-benzylguanine against the wild-type alkyltransferase and is even capable of inactivating the

90 inding proteins, agt for an alkyl-cysteine-S-alkyltransferase and Taf1 for a subunit of transcription

91 ery slow, whereas the E. coli Ogt, the human alkyltransferase, and the mutant A316P/W336A-Ada-C alkyl

92 Determination of O6-alkylguanine-DNA-alkyltransferase (AT) activity revealed that astrocytes

93 Depletion of O6-alkylguanine-DNA-alkyltransferase (ATase) activity confers only limited i

94 A human O6-alkylguanine-DNA-alkyltransferase (ATase) cDNA-containing retrovirus was

95 O6-BeG) on the levels of O6-alkylguanine-DNA alkyltransferase (ATase) in the hematopoietic compartmen

96 The DNA repair protein, O6-alkylguanine-DNA alkyltransferase (ATase) is an important cellular resist

97 The expression of O6-alkylguanine-DNA-alkyltransferase (ATase), which is responsible for repai

98 Inactivation of O6-alkylguanine-DNA alkyltransferase by O6-benzylguanine renders tumor cells

99 Inactivation of alkyltransferase by the gamma-folate ester of O6-[4-(hyd

100 ersible inactivator of O(6)-alkylguanine-DNA alkyltransferase currently in clinical trials to overcom

101 V139F provided alkyltransferase-deficient bacteria with greater protect

102 rary was selected for the ability to provide alkyltransferase-deficient Escherichia coli with resista

103 lkyl-accepting cysteine was transformed into alkyltransferase-deficient Escherichia coli.

104 dence supports the existence of at least two alkyltransferase-dependent pathways for 1,2-dibromoethan

105 est that the enzyme belongs to the family of alkyltransferase enzymes for which Zn plays a key role i

106 est that the enzyme belongs to the family of alkyltransferase enzymes for which Zn plays a role in ac

107 here in vitro and in vivo studies on the DNA alkyltransferase from the thermophilic archaeon Sulfolob

108 olymorphism in the human O6-alkylguanine-DNA alkyltransferase gene exists, with about 15% of the popu

109 Patients with high levels of alkyltransferase had shorter time to treatment failure (

110 y of recombinant human O(6)-alkylguanine-DNA alkyltransferase (hAGT) revealed a previously unknown zi

111 site cysteine of human O(6)-alkylguanine-DNA alkyltransferase (hAGT), Cys145, was shown to be highly

112 ssion of human protein O(6)-alkylguanine-DNA alkyltransferase (hAGT), in which the active site cystei

113 f the DNA repair protein O6-alkylguanine-DNA alkyltransferase in brain tumors was correlated with res

114 The level of alkyltransferase in CD34+ cells or in mononuclear BM cel

115 A high level of the alkyltransferase in many tumour cells renders them resis

116 rotein O6-alkylguanine-DNA alkyltransferase (alkyltransferase) in vitro than O6-benzylguanine, the pr

117 ise as an agent for possible tumor-selective alkyltransferase inactivation, which suggests it may pro

118 n vitro than O6-benzylguanine, the prototype alkyltransferase inactivator currently in clinical trial

119 provide a new class of highly water-soluble alkyltransferase inactivators and form the basis to cons

120 O6-benzyl-2'-deoxyguanosine were more potent alkyltransferase inactivators than the parent nucleoside

121 t the DNA repair protein O6-alkylguanine-DNA alkyltransferase increases the mutagenicity of 1,2-dibro

122 wer in the cells lacking O6-alkylguanine-DNA alkyltransferase, indicating that O6-MeG was causally in

123 n with dual-dose O6-benzylguanine to prolong alkyltransferase inhibition could reach an MTD.

124 ustine (BCNU) plus the O(6)-alkylguanine-DNA alkyltransferase inhibitor O(6)-benzylguanine (O(6)-BG)

125 lguanine and that higher doses or additional alkyltransferase inhibitors capable of inactivating this

126 In order to produce more soluble alkyltransferase inhibitors, we have synthesized three a

127 The human alkyltransferase is inactivated by the free base O6-benz

128 The alkylated form of DNA alkyltransferases is inactive, and in eukaryotes, it is

129 ne (BG), an inhibitor of O6-alkylguanine-DNA alkyltransferase, is being tested clinically for its abi

130 illing of tumors that express high levels of alkyltransferase, it would also be expected to reduce th

131 horionic gonadotropin-O(6) -alkylguanine-DNA alkyltransferase) led to LAMP-to-hCG signal transduction

132 ld encourage prospective studies to evaluate alkyltransferase levels as a method, for identifying bra

133 Alkyltransferase levels in individual cells in sections

134 d also be expected to reduce the already low alkyltransferase levels of hematopoietic stem cells and,

135 , survival was more strongly correlated with alkyltransferase levels than with age.

136 er of importance, were age, tumor grade, and alkyltransferase levels.

137 ate 1.7 times greater than patients with low alkyltransferase levels.

138 The alkyltransferase-like (ATL) proteins contain primary seq

139 Alkyltransferase-like (ATL) proteins in Schizosaccharomy

140 reveal the presence of a group of proteins [alkyltransferase-like (ATL) proteins] showing amino acid

141 ccharomyces pombe a DNA recognition protein, alkyltransferase-like 1 (Atl1), can play a pivotal role

142 Alkyltransferase-like enzymes mark O(6)-alkylguanine les

143 R process in those organisms that express an alkyltransferase-like gene and raise the question of whe

144 Alkyltransferase-like proteins (ATLs) are a novel class

145 Alkyltransferase-like proteins (ATLs) share functional m

146 O6-alkylguanine DNA-alkyltransferase mediated DNA repair effectively blocks

147 The alkyltransferase-mediated mutations produced by 1,2-dibr

148 ase currently in clinical trials to overcome alkyltransferase-mediated resistance to certain cancer c

149 O(6)-Alkylguanine-DNA alkyltransferase (MGMT) is the sole repair protein for O

150 Human O(6)-alkylguanine-DNA alkyltransferase (MGMT) repairs potentially cytotoxic an

151 bited the transfer of methyl groups to human alkyltransferase (MGMT).

152 s in DNA are repaired by O6-alkylguanine-DNA alkyltransferases (MGMT) by transfer of the alkyl group

153 D34+) hematopoietic precursors do not induce alkyltransferase, myelosuppression may be the dose-limit

154 cell function include O(6)-alkylguanine DNA alkyltransferase, nucleotide excision repair, base excis

155 Unlike the Escherichia coli alkyltransferase Ogt that also repairs O(4)-methylthymin

156 This mutant human alkyltransferase, or others similarly created and select

157 SNAP or CLIP forms of O(6)-alkylguanine-DNA-alkyltransferase plus a peptide epitope tag.

158 em cells with a gene encoding a BG-resistant alkyltransferase prior to BG/alkylation treatment.

159 DNA repair alkyltransferases protect organisms against the cytotoxi

160 ine lesions that are poor substrates for the alkyltransferase proteins in higher eukaryotes might, by

161 se found in DNA repair O(6)-alkylguanine-DNA alkyltransferase proteins.

162 olecular modeling of their interactions with alkyltransferase provided a molecular explanation for th

163 are better substrates than methyl groups for alkyltransferases provided that steric factors do not pr

164 O6-Alkylguanine-DNA alkyltransferase (alkyltransferase) provides an important source of resist

165 ransferase, and the mutant A316P/W336A-Ada-C alkyltransferases reacted very rapidly with this 16-mer

166 previous proposal that AANAT can catalyze an alkyltransferase reaction in a conformationally altered

167 The alkyltransferase removes the alkyl group to one of its o

168 A repair protein human O(6)-alkylguanine-DNA alkyltransferase repairs lesions at the 5' ends of 70-nu

169 genicity, one involving Gua N7-alkylation by alkyltransferase-S-CH2CH2Br and depurination, plus anoth

170 e we report the X-ray structure of the human alkyltransferase solved using the technique of multiple

171 effective in preventing mutations than human alkyltransferase, suggesting that the endonuclease V act

172 s of O(6)-alkylguanine-DNA alkyltransferase (alkyltransferase) than either O(6)-benzylguanine or O(6)

173 ven capable of inactivating the P140K mutant alkyltransferase that is resistant to inactivation by O6

174 A pool of 6.5 x 10(6) human alkyltransferases that were randomly mutated at six amin

175 to interpret the behaviour of certain mutant alkyltransferases to enhance biochemical understanding o

176 d in epoxide carboxylation: a zinc-dependent alkyltransferase, two short-chain dehydrogenases with sp

177 e subpopulation of cells with high levels of alkyltransferase was correlated directly with drug resis

178 ffering only in level of O6-alkylguanine-DNA alkyltransferase, were treated with MNU and assayed for

179 ta-position greatly enhances inactivation of alkyltransferase, whereas para-substitution has little e

180 ng for a mutant version of O(6)-alkylguanine alkyltransferase, which is efficiently assembled with an

181 the DNA repair protein, O6-alkylguanine-DNA alkyltransferase, which may explain their susceptibility

182 ors capable of inactivating this form of the alkyltransferase will be necessary.

183 cubation of Escherichia coli-expressed human alkyltransferase with 1,2-dibromoethane and single-stran

184 phan, or alanine on the interaction of human alkyltransferase with O6-benzylguanine using direct dete