ライフサイエンスコーパス: allantois

1 lateral plate mesoderm, and the base of the allantois.

2 ion along the proximodistal axis of the host allantois.

3 h abnormal notochord, posterior somites, and allantois.

4 show a mild, transient growth defect in the allantois.

5 in a manner independent of proximity to the allantois.

6 required for the formation of both PGCs and allantois.

7 survival and for the differentiation of the allantois.

8 olk sac contributes endothelial cells to the allantois.

9 ions, and behavior resembled those of intact allantoises.

10 abundant extra-embryonic mesoderm, including allantois, a rudimentary heart and middle primitive stre

11 three cell types have been identified in the allantois: an outer layer of mesothelial cells, whose di

12 Bmp2 homozygous embryos also have a short allantois and about 50% of them do not undergo normal ch

13 it is separated physically from PrP-positive allantois and chorioallantois by PrP-negative amnion.

14 show that the hematopoietic potential of the allantois and chorion does not require their union, indi

15 Here, we show that the allantois and chorion, isolated prior to the establishme

16 they, and other ACD cells, contribute to the allantois and fetal tissues.

17 equired for normal PGC motility, both in the allantois and in the hindgut.

18 re distal than basal core cells in the early allantois and never in mesothelium.

19 except in cells at the interface between the allantois and the ectoplacental plate.

20 ntrast, vasculogenesis proceeded in both the allantois and the embryo proper.

21 s to be independent of erythropoiesis in the allantois and to involve a distal-to-proximal gradient i

22 ts were significantly decreased in T(C)/T(C) allantoises and did not coalesce into endothelial tubule

23 Germ cells migrate actively in the allantois, and move directionally from the allantois int

24 e established by mechanisms intrinsic to the allantois, and possibly include roles for cell age and c

25 ing the chorion, yolk sac blood islands, and allantois appear to develop normally, the small embryoni

26 lacZ-expressing headfold-stage allantoises (approximately 8.0 days postcoitum; dpc) wer

27 mine whether the blood vessels of the murine allantois are formed by vasculogenesis or angiogenesis.

28 Finally, we discuss the potential of the allantois as a model system to provide insights into dis

29 ses resulted in the formation of regenerated allantoises at all time points.

30 development, microsurgery was used to remove allantoises at ten developmental stages.

31 ved extraembryonic structures, including the allantois, blood islands of the yolk sack, primordial ge

32 s were allocated properly at the base of the allantois, but their cell expansion was progressively im

33 nzidine-stained cells were observed in donor allantoises, but none contained silver grains above back

34 Rather, all of them resembled intact allantoises by morphological, molecular and functional c

35 n alpha5-null embryonic yolk sac, amnion and allantois compared with wild-type, indicating that the m

36 ssion of Zfp36L1 at E8.0 was greatest in the allantois, consistent with a potential role in chorioall

37 Finally, in vitro allantois cultures demonstrated a requirement for PDGF s

38 zed, and VCAM-1 protein, a marker for distal allantois development, is not expressed.

39 In this study, we have investigated how the allantois differentiates, with the goal of discriminatin

40 Endothelial cells within mutant allantoises do not undergo vascular remodeling.

41 lopmental potency of cells within the murine allantois during gastrulation.

42 on is required for fusion of the chorion and allantois during placental development.

43 Using the cultured mouse allantois explant model of blood vessel formation, we fo

44 sed PECAM+ cell clusters to form in cultured allantois explants from normal mice.

45 sitive (PECAM+) cells formed within cultured allantois explants from VE-cadherin null embryos.

46 serum to promote vasculogenesis in cultured allantois explants.

47 In Smad1 mutant mice, the allantois fails to fuse to the chorion, resulting in a l

48 ive cells begins in the distal region of the allantois, farthest from the streak.

49 y Smad1(-/-) phenotypes, including defective allantois formation and the lack of primordial germ cell

50 mately 9.5 days postcoitum due to defects in allantois formation.

51 eficient embryos show a marked impairment in allantois formation.

52 n isolation, neural plate and headfold-stage allantoises formed a conspicuous vascular network that w

53 toic placenta and umbilical circulation, the allantois frequently is overlooked in embryologic studie

54 r remodeling, and angiogenesis are essential allantois functions in the establishment of the chorioal

55 airway and vascular development and chorion-allantois fusion during placental development.

56 embryonic development accompanied by lack of allantois fusion to the chorion and increased degenerati

57 DiI labeling further revealed that isolated allantoises grew and vascularized in the absence of sign

58 In this study, the extent to which explanted allantoises grow and differentiate outside of the concep

59 During gastrulation, the allantois grows into the exocoelomic cavity as a mesoder

60 (head and branchial arches) and less severe (allantois growth) than the null.

61 s studies, growth and differentiation of the allantois had been elucidated in whole embryos.

62 BMP signaling during the development of the allantois, heart, branchial arches, somites and forebrai

63 review blood vessel formation in the murine allantois, highlighting the expression of genes and invo

64 Second, fewer PGCs are found in the allantois in Steel-null embryos, but this is not due to

65 E functions to induce precursors of PGCs and allantois in the adjacent epiblast, resulting in complet

66 e allantois, and move directionally from the allantois into the proximal epiblast.

67 The base of the allantois is also thought to contain the future germ lin

68 Hence, the vasculature of the allantois is formed intrinsically by vasculogenesis rath

69 The allantois is the embryonic precursor of the umbilical co

70 The murine allantois is the future umbilical component of the place

71 imitive streak and an intrinsic role for the allantois itself.

72 ot affect the establishment of either PGC or allantois lineages, but is required for PGC localization

73 her, our data reveal that the headfold-stage allantois may contain a proximodistal gradient of differ

74 ozygotes die by E10.5 and display defects in allantois morphogenesis, cardiac looping and primordial

75 oic regions were placed into the base of the allantois of host conceptuses.

76 In addition, the allantois of many Smad5 mutants is fused to the chorion,

77 bx2, is reduced in both the hindlimb and the allantois of Tbx4-mutant embryos prior to the developmen

78 The allantoises of Tbx4-mutant embryos are stunted, apoptoti

79 ects in multiple organ systems including the allantois, placental vasculature, ventral body wall, eye

80 rulation-stage primitive streak, chorion and allantois precursors, respectively.

81 embryonic mesoderm (ExM), in which the PGCs, allantois primordium, and angioblasts are first detected

82 oic placenta forms through the fusion of the allantois (progenitor tissue of the umbilical cord), wit

83 Nevertheless, a small number of allantoises removed from conceptuses at a considerably e

84 ollapsed embryonic cavity, the absence of an allantois, retarded mesodermal migration, and increased

85 techniques available for manipulation of the allantois that are unavailable for yolk sac or dorsal ao

86 in brachyury (T) exhibit a short, misshapen allantois that fails to fuse with the chorion.

87 a model of mesodermal differentiation in the allantois that is position- and possibly age-dependent.

88 ere made chimeric with lacZ-expressing donor allantoises that were additionally labeled with [3H]meth

89 oderm derivatives including the chorion, the allantois, the amnion and a subset of endothelial cells.

90 5-8 dpc) in the embryo proper, yolk sac, and allantois, the S1P receptor S1P(2) is expressed in conju

91 gh vasculogenesis is initiated in the mutant allantois, the vessels formed are disorganized, and VCAM

92 that the streak contributes mesoderm to the allantois throughout the latter's early development, mic

93 in the amnion, likely due to mislocation of allantois tissue.

94 ing cells from their first appearance in the allantois to the time they enter the genital ridges.

95 od vessels were not found in the base of the allantois until 4-somite pairs had formed in the fetus (

96 e erythroid cells were not identified in the allantois until 6-somite pairs when continuity between i

97 Explanted allantoises vascularized with distal-to-proximal polarit

98 ntiation along the proximodistal axis of the allantois were further borne out when the three allantoi

99 Early headfold-stage murine allantoises were explanted directly onto tissue culture

100 oid cells could be of allantoic origin, host allantoises were made chimeric with lacZ-expressing dono

101 When allantoises were removed from contact with the streak, n

102 ouse gastrulation, including the core of the allantois, where its function is not known.

103 lls manifested a profoundly swollen/hydropic allantois, which failed to fuse with the chorion, a phen

104 r network begins in the distal region of the allantois, which is most remote from other tissues, as e

105 conceptus, little is known about the murine allantois, which will become the umbilical cord of the c

106 t Smad1 is important in the formation of the allantois, while Smad5 has been shown to be critical in

107 The murine allantois will become the umbilical artery and vein of t

108 Recent evidence has suggested that the allantois, within which the PGCs temporarily take up res