ライフサイエンスコーパス: allospecific

1 1 and Th2 cytokine network and systemic host allospecific Ab (allo-Ab) responses in the development o

2 notypically hostile NK cells that express an allospecific activating receptor without coexpressing an

3 vitro study quantified CD103+ T cells after allospecific activation with and without exogenous TGFbe

4 e findings highlight surprisingly asymmetric allospecific alterations in iNKT cells as they develop a

5 not be of donor origin, suggesting that both allospecific and non-allospecific mechanisms regulate en

6 freshly prepared primary MLRs, to determine allospecific and nonspecific inhibitory and Treg recruit

7 to investigate how immunosuppression affects allospecific and pathogen-specific memory B cells, and r

8 mulated with HLA class I antibody (W6/32) or allospecific antibodies from sensitized patients (n=6) w

9 Importantly, allospecific antibodies from sensitized patients also ac

10 f this study was to test the contribution of allospecific antibody-secreting cells (ASCs) from differ

11 (r=0.682, P=0.005) and inversely with IR of allospecific, antiinflammatory, CTLA4(+)Tc-M (r=-0.638,

12 Suppression was allospecific, as Treg cells induced by third-party antig

13 e inability of the direct pathway to provide allospecific B cell help.

14 alloantigen, processed and presented by the allospecific B cell.

15 alloantibody responses, with splenic GCs and allospecific bone marrow plasma cells readily detectable

16 estigated the development and maintenance of allospecific CD103 T cells within the tubular microenvir

17 sayed for allospecific CD154CD19 B cells and allospecific CD154 TcM in 16-hr live-cell mixed leukocyt

18 ed significantly with IR of proinflammatory, allospecific CD154(+)Tc-M (r=0.682, P=0.005) and inverse

19 Allospecific CD154+T-cytotoxic memory cells (CD154+TcM)

20 ated monocytes correlated significantly with allospecific CD154+T-cytotoxic memory cells (Spearman r=

21 ng rATG concentrations, proportionately more allospecific CD154+T-cytotoxic memory cells (TcM) surviv

22 ic analyses, the rejection-risk threshold of allospecific CD154+T-cytotoxic memory cells (TcMs) assoc

23 Allospecific CD154+T-cytotoxic memory cells predict acut

24 Allospecific CD154+TcM correlated inversely with CTLA4+T

25 The strong association between ACR and allospecific CD154+TcM may be useful in minimizing proto

26 han TcM, resulting in relative enrichment of allospecific CD154+TcM.

27 Samples were assayed for allospecific CD154CD19 B cells and allospecific CD154 Tc

28 Allospecific CD154CD19 B cells identify rejection-prone

29 The IR of allospecific CD154CD19 B cells more than or equal to 1.3

30 d to the semi-direct pathway, as measured by allospecific CD4 (indirect) and CD8 T-cell clones (direc

31 attractants necessary for the recruitment of allospecific CD4 T cells into the graft.

32 Activation and cytokine secretion by allospecific CD4+ and CD8+ T cells were initially blocke

33 fer model of TCR transgenic T cells to track allospecific CD4+ T cell expansion and trafficking chara

34 Type 2 immune deviation of allospecific CD4+ T cells resulted in IL-4 and IL-5 prod

35 escribed factors affecting the generation of allospecific CD4CD25 forkhead/winged helix transcription

36 Further studies demonstrated that these allospecific CD4high cells were also present (< or = 1%

37 ergized to promote expansion and survival of allospecific CD8 CD103 T cells in vitro, but IL-15 down-

38 ions can strongly stimulate both NK cell and allospecific CD8 T cell responses, and these same effect

39 These results suggest that activated, allospecific CD8 T cells are recruited to tubules during

40 and adhesion molecules upon interaction with allospecific CD8 T suppressor cells or exposure to inhib

41 as the cytotoxic effector mechanisms used by allospecific CD8(+) cytolytic T cells.

42 The magnitude of allospecific CD8(+) T cell in vivo cytotoxic effector fu

43 Allospecific CD8(+) T lymphocytes are an important compo

44 -1 reduced cytokine-induced proliferation of allospecific CD8+ cloned L3 T cells and OVA-reactive CD4

45 cytokine requirements for the generation of allospecific CD8+ CTL in vitro and have found that IL-4

46 he development, function, and persistence of allospecific CD8+ T cell effectors in vivo.

47 In this study, we tested the role of IL-2 in allospecific CD8+ T cell memory by analyzing the long-te

48 endent inhibitory effect on proliferation of allospecific CD8+ T cells in mixed lymphocyte culture.

49 Adoptive transfer of allospecific CD8+ T cells purified 14 days postburn resu

50 entical type 2 cytokine-inducing conditions, allospecific CD8+ T cells were primed to become IL-4, IL

51 We asked whether allospecific CD8+ T cells, in the absence of CD4+ T cell

52 Allospecific cell lines generated from GNLY transgenic a

53 Allospecific cell lines were preincubated with anti-alph

54 95-ligand pathway; therefore the deletion of allospecific cells by donor spleen cells may be induced

55 1 partially protects HUVECs against lysis by allospecific class I MHC-restricted cytolytic T lymphocy

56 he differentiation of purified CD8+ PBL into allospecific, class I MHC-restricted CTL that lyse EC, b

57 PCR assays to monitor the levels of putative allospecific clonotypes in posttransplant blood samples

58 tosis by sHLA was analyzed by adding sHLA to allospecific CTL 4 or for 24 hr before flow cytometric a

59 This rapid generation of allospecific CTL activity during a viral infection prece

60 ime course studies showed that DSBM impaired allospecific CTL activity whether given on day -10 (3.3%

61 tion, they show that both CD4(+)- and CD8(+)-allospecific CTL can be isolated from rejected allogenic

62 Allospecific CTL can function as cellular effectors of s

63 ells, implying that soluble antigen promotes allospecific CTL death.

64 Thus, (1) DSBM inhibits allospecific CTL development in the allograft, (2) decre

65 nto F(1) hosts stimulates the development of allospecific CTL effectors that eliminate host lymphocyt

66 By providing exogenous cytokines to allospecific CTL generation cultures, we further demonst

67 neal cells produced a profound inhibition of allospecific CTL, DTH, and mixed-lymphocyte responses.

68 ells produces a dose-dependent inhibition of allospecific CTL, DTH, and mixed-lymphocyte responses.

69 costimulation blockade are able to generate allospecific CTL- and IFN-gamma-producing T cells within

70 regulate differentiation and/or expansion of allospecific CTL.

71 of CD8+, but anti-CD8-resistant, MHC class I-allospecific CTL.

72 s, providing evidence that the generation of allospecific CTLs during acute LCMV infection is antigen

73 ed, cardiac allografts and the generation of allospecific CTLs were not impaired in the absence of IL

74 ent donor cells differentiated normally into allospecific CTLs.

75 yme-linked immunospot assay for detection of allospecific cytokines produced by individual human PBLs

76 roliferative responses and the generation of allospecific cytolytic effectors.

77 and engage MHC class I antigens, leading to allospecific cytolytic responses and graft rejection.

78 HC-SMA were assessed for the development of allospecific cytolytic T cells (allo-CTLs).

79 BM infusion significantly reduced intragraft allospecific cytolytic T-cell (CTL) activity compared wi

80 alloantibody levels correlated with in vivo allospecific cytotoxic activity in CD8 knockout hepatocy

81 Moreover, the generation of allospecific cytotoxic activity is inhibited by IL-10.

82 ng the 19 clones analyzed: 1) TCR alphabeta+ allospecific cytotoxic cells, 2) TCR alphabeta+ nonspeci

83 cific cytotoxic cells, and 5) TCR alphabeta- allospecific cytotoxic cells.

84 mulated the proliferation and development of allospecific cytotoxic effectors in vitro and in vivo.

85 The demonstration of cloned, TCR alphabeta+, allospecific cytotoxic effectors provides the strongest

86 Administering IL-18 significantly enhanced allospecific cytotoxic function and expansion of CD8(+)

87 Moreover, mdr1a-/- T cells produced strong allospecific cytotoxic responses comparable to those of

88 alized splenic T lymphocyte subsets, reduced allospecific cytotoxic T lymphocyte activity, and increa

89 PG27 reduced day 7 splenic allospecific cytotoxic T lymphocyte levels by more than

90 from control rats exhibited greater than 35% allospecific cytotoxicity 8 days after grafting.

91 Here, we demonstrate that robust allospecific cytotoxicity is also manifest in vivo.

92 The development of in vivo allospecific cytotoxicity was determined by clearance of

93 reased nuclear NFkappaB in T cells and their allospecific cytotoxicity.

94 of these CD4+ T cell clones displayed strong allospecific cytotoxicity.

95 injected s.c. into naive recipients induced allospecific delayed hypersensitivity and elicited delay

96 Graft rejection, induction of allospecific delayed-type hypersensitivity (DTH) respons

97 Allospecific delayed-type hypersensitivity (DTH) was eva

98 Graft survival and allospecific delayed-type hypersensitivity (DTH) were us

99 ition to graft survival, graft infiltration, allospecific delayed-type hypersensitivity (DTH), and cy

100 raft rejection and prevent the generation of allospecific delayed-type hypersensitivity (DTH).

101 Furthermore, allospecific delayed-type hypersensitivity and gene expr

102 Additionally, allospecific delayed-type hypersensitivity was compared

103 ally, all CLN(-) hosts failed to demonstrate allospecific DTH (P < 0.001).

104 -1-/- mice receiving B10.D2 grafts developed allospecific DTH.

105 d untreated WT mice, as was the induction of allospecific DTH.

106 de future investigations of prenatal NK cell-allospecific education.

107 T cells as helper cells in the generation of allospecific effector macrophages in corneal graft rejec

108 ransgenic model, blockade of TIM-3 increased allospecific effector T cells, enhanced Th1 and Th17 pol

109 , to assess the capacity of TRegs to mediate allospecific effects.

110 However, DNA fragmentation induced by either allospecific FasL-defective CTL, or by perforin-deficien

111 ce from animal studies has demonstrated that allospecific FoxP3(+)CD4(+) regulatory T (Treg) cells ex

112 Allospecific GVL reactivity was reduced but not eliminat

113 results indicate the importance of adequate allospecific helper as well as effector T cells for the

114 igen, we identified and cloned a TCR from an allospecific HLA-A2-restricted, HCV:NS3:1406-1415-reacti

115 We therefore conclude that allospecific human CD8+ T cells of Tc1 and Tc2 phenotype

116 accompanied by markedly increased numbers of allospecific, IFN-gamma-producing cells in the periphery

117 In contrast, allospecific IgM and IgG were significantly decreased in

118 In contrast, there was no impairment of the allospecific IgM response.

119 lockade, there was a diminished frequency of allospecific IL-10-producing cells and an increased freq

120 proved ability to define the strength of the allospecific immune response by enzyme-linked immunospot

121 Nevertheless, an allospecific immune response could occur as a consequenc

122 by HFRPE cells may not induce a significant allospecific immune response.

123 Though T cells clearly mediate allospecific immunity, the manner in which reperfusion e

124 the anti-CD154-treated mice did not exhibit allospecific immunity.

125 atibility complex disparate skin grafts, the allospecific immunoglobulin (Ig)G response was markedly

126 activating receptor without coexpressing any allospecific inhibitory receptors.

127 skin transplantation, increased induction of allospecific iTregs and a reduction in T effector respon

128 The CD8(+) Ly49G2(+) population mediated no allospecific killing, nor was any NK-like killing observ

129 t the CD103+ and CD103- subsets both possess allospecific lytic activity.

130 e and prolong islet allograft survival in an allospecific manner.

131 n, suggesting that both allospecific and non-allospecific mechanisms regulate engraftment.

132 itro analyses revealed that the apoptosis of allospecific memory CD8(+) T cells is significantly incr

133 Transgenic T cells were used to track allospecific memory T-cell generation.

134 We have previously shown that allospecific murine CD8+ T cells of the Tc1 and Tc2 phen

135 nswer this question, we investigated whether allospecific naive or memory T cells can mediate acute c

136 Tregs rather than prevention of expansion of allospecific natural Tregs.

137 , cell-extrinsic signals appeared to dictate allospecific patterns of Ly49 receptor expression and li

138 -restricted T cell hybridoma (B4.2.3), or an allospecific, peptide-dependent, T cell hybridoma (3DT52

139 tic function, and clonally deleted host-type allospecific precursor CTL in vitro.

140 in both CD4(+) and CD8(+) T cells, including allospecific proliferation and cytokine secretion.

141 0-producing CD8 T cells strongly inhibit the allospecific proliferation of naive CD8 T cells to monoc

142 y, fully mismatched graft were performed and allospecific proliferation was measured after depletion

143 ctions, so as to immunologically monitor the allospecific regulation after transplantation.

144 These results describe a novel allospecific regulatory CD8(+)PD1(+) T cell induced by I

145 is assay can thereby be helpful in assessing allospecific regulatory effects of diverse immunosuppres

146 oughout the overall T-cell repertoire, one's allospecific repertoire is similarly made up of cells in

147 man cells and tested agents known to inhibit allospecific responses for their ability to inhibit xeno

148 ess whether such a construct could affect Dd allospecific responses in vitro and in vivo.

149 grafts, the CD8+ Vbeta8+ cells demonstrated allospecific responses that were numerically larger than

150 d acute lung allograft rejection and reduced allospecific responsiveness by markedly decreasing monon

151 ation in the settings of both polyclonal and allospecific stimulation.

152 Cultured Tregs produced allospecific suppression, maintained demethylation of th

153 this drug interfere with the persistence of allospecific suppressor cells for 35 days after the graf

154 Similar to allospecific suppressors, these xenospecific suppressor

155 When combined with allospecific T and B cells, this information may differe

156 )) phenotype and were capable of suppressing allospecific T cell proliferation and IFN-gamma producti

157 eks following rejection, however, the memory allospecific T cell response became predominant in the r

158 of C5aR in both graft and recipient reduced allospecific T cell responses and prolonged renal allogr

159 T cells modulates their function, enhancing allospecific T cell responses that lead to allograft rej

160 n and subsequently enhances its capacity for allospecific T cell stimulation.

161 llo-Ab responses and induction of peripheral allospecific T cell unresponsiveness both in vitro and i

162 g of donor Th2 cell therapy, host-anti-donor allospecific T cells acquired Th2 polarity, persisted po

163 Indirect allospecific T cells activated by such CD11c(+) dendriti

164 olecules and were less potent at stimulating allospecific T cells after an additional 48-hour culture

165 iminished frequencies of IFN-gamma-producing allospecific T cells and reduced CTL activity.

166 tible with either deletion or suppression of allospecific T cells as possible mechanisms of tolerance

167 the DLN whether it contains naive or memory allospecific T cells as shown in experiments in which re

168 alysis of the phenotype and proliferation of allospecific T cells expanded in vitro in the presence o

169 It has been speculated that allospecific T cells express high-affinity T cell recept

170 directed to tissue antigens but differ from allospecific T cells in several important respects, refl

171 eneic bone marrow cells on the activation of allospecific T cells in vitro.

172 In contrast, direct allospecific T cells interacting with intact donor ECDI-

173 ining demonstrated that early recruitment of allospecific T cells into allografts around POD10 correl

174 ascularization and that early recruitment of allospecific T cells into the grafts promotes destructio

175 cells and/or soluble MHC Ags suppresses NIMA-allospecific T cells of the offspring, predisposing to o

176 ively, these results suggest that peripheral allospecific T cells undergo the initial stages of activ

177 These innate immune cells, in addition to allospecific T cells, can damage cardiomyocytes directly

178 To target indirect allospecific T cells, ECDI-SPs induce upregulation of ne

179 their ability to act as stimulatory APCs for allospecific T cells.

180 sequently have reduced capacity to stimulate allospecific T cells.

181 ompromised capacity of the cells to activate allospecific T cells.

182 Allospecific T effector cells were induced in all mice u

183 llorecognition is responsible for generating allospecific T follicular helper cells remains unclear.

184 Moreover, allospecific T regulatory (Tr) cells correlated with acc

185 ivity acquired by dendritic cells exposed to allospecific T suppressor (Ts) cells.

186 We analyzed individual allospecific T-cell clones derived from a Wiskott-Aldric

187 CMV characteristically induces an anti-H2(d) allospecific T-cell response that includes T-cell clones

188 T-cell costimulatory blocking agents inhibit allospecific T-cell responses in vitro and prevent allog

189 cooperation, CD154 has been used to identify allospecific T-cytotoxic memory cells (TcM) for rejectio

190 These T cells are analogous to the "allospecific" T cells that have been described in hemato

191 R alphabeta+ nonspecific cytotoxic cells, 3) allospecific TCR alphabeta+ noncytotoxic cells, 4) TCR a

192 epends on the ability to block generation of allospecific Th1 responses that lead to rejection.

193 ) infected with influenza A virus have lower allospecific Th1-cell stimulatory abilities than DCs act

194 s also developed vigorous primary and memory allospecific Th1/Th2 responses that exceeded the respons

195 , because neonatal antigen exposure triggers allospecific Th2 CD4 memory cells, whereas antigen expos

196 her show that LCMV prevents the induction of allospecific tolerance and mixed hematopoietic chimerism

197 es, without CD40-CD40L costimulation, induce allospecific tolerance but may trigger allo-independent

198 ade is most effective at inducing long-lived allospecific tolerance if anergy and regulation can be e

199 ese findings illustrate the robust nature of allospecific tolerance in prenatal mixed chimerism compa

200 Moreover, it is uncertain whether any allospecific tolerance occurring with CD40-CD40L blockad

201 CD40L-/- transplant recipients developed allospecific tolerance to the donor haplotype; second se

202 as an efficacious cellular therapy to induce allospecific tolerance to transplantation antigens.

203 mphocytic choriomeningitis virus on prenatal allospecific tolerance was examined.

204 to induce mixed hematopoietic chimerism and allospecific tolerance.

205 tive NKT cells are required for induction of allospecific Tr cells and are essential for survival of

206 B/c mice, and the adoptive transfer of these allospecific Tr cells to Jalpha281 KO mice allowed a 50%

207 Adoptive transfer of allospecific transgenic CD4 T cells revealed a "split to

208 MMc) has been associated with development of allospecific transplant tolerance, antitumor immunity, a

209 established for selective expansion of human allospecific Treg cells ex vivo.

210 pamycin inhibitors have disparate effects on allospecific Treg generation using the Treg MLR.

211 s has demonstrated that adoptive transfer of allospecific Tregs offers greater protection from graft

212 n a decreased frequency of overall number of allospecific Tregs.

213 ments, and function of alloantigen-specific (allospecific) Tregs from human blood.

214 kin resulted in graft rejection, with stable allospecific type 2 cytokine production in vivo.