ライフサイエンスコーパス: allosteric site

1 refined to recognize lorazepam in a putative allosteric site.

2 ut not ppGpp-positively regulates SAS1 at an allosteric site.

3 AChR by loop II without penetration into the allosteric site.

4 compounds acting at a common, transmembrane allosteric site.

5 tly with the orthosteric site and part of an allosteric site.

6 he active site or occupation of a regulatory allosteric site.

7 active site of PBP2a within the core of the allosteric site.

8 eatures regulate access of AZ10606120 to the allosteric site.

9 crease in NADP-ME2 activity by binding to an allosteric site.

10 lecules activate relaxin receptor through an allosteric site.

11 ibition potency supporting recognition of an allosteric site.

12 site and suggests the presence of a second, allosteric site.

13 ition in switch II upon ligand binding at an allosteric site.

14 etects the binding of ligands to this unique allosteric site.

15 eracts with muscarinic receptors via a novel allosteric site.

16 ligands that compete with each other at the allosteric site.

17 laxation times, one of which arises from the allosteric site.

18 turbations at the active site, 15 A from the allosteric site.

19 of bicarbonate from the bulk solution to its allosteric site.

20 ains bind bicarbonate ion exclusively at the allosteric site.

21 627, suggesting a potential location for the allosteric site.

22 , an HspQ binding site and an HspQ-modulated allosteric site.

23 mOctR, and alpha2C-adrenergic receptor at an allosteric site.

24 red by an inability to precisely control the allosteric site.

25 rs depending on what dNTP is bound in the A2 allosteric site.

26 bstrate ATP, indicating that they act via an allosteric site.

27 Ras.GDP cannot properly activate Sos at the allosteric site.

28 opriate model receptor for investigating the allosteric site.

29 ds designed to bind irreversibly to the CB1R allosteric site.

30 (176), a trigger between the active site and allosteric site.

31 ed by the binding of an effector to a distal allosteric site.

32 ected by different dNTP ligands bound in the allosteric site.

33 with two orthosteric binding modes and to an allosteric site.

34 ed that they most likely used an overlapping allosteric site.

35 state of the protein when they bind to their allosteric sites.

36 dulate nAChR function by binding to distinct allosteric sites.

37 involving binding to both the catalytic and allosteric sites.

38 all molecules that are able to interact with allosteric sites.

39 r Cys-loop receptors, by binding to distinct allosteric sites.

40 c activity by dGTP binding at four identical allosteric sites.

41 effective inhibitors and help identify novel allosteric sites.

42 r energetic coupling with Phe binding in the allosteric sites.

43 odulation by ligands acting through numerous allosteric sites.

44 he highly conserved ACh-binding site, termed allosteric sites.

45 rough the orthosteric site but by binding to allosteric sites.

46 d to both the dimerization interface and the allosteric sites.

47 eted as tight binding at both the active and allosteric sites.

48 enesis, also supports the existence of these allosteric sites.

49 le in ligand binding to both orthosteric and allosteric sites.

50 vation and variability within the identified allosteric sites.

51 e and also drive the identification of novel allosteric sites.

52 ion of cooperativity between orthosteric and allosteric sites.

53 e for high-throughput searches for candidate allosteric sites.

54 control of protein function by modulation of allosteric sites.

55 D1 is activated by binding of GTP or dGTP at allosteric site 1 and a dNTP of any type at allosteric s

56 allosteric site 1 and a dNTP of any type at allosteric site 2.

57 Moreover, ATP and dATP bind at an additional allosteric site 40 A away from loop 2 and thereby drive

58 ested that tetramic acid binds to UPPS at an allosteric site adjacent to the FPP binding site.

59 ic EF1143 contains four additional secondary allosteric sites adjacent to the previously identified d

60 d potency derived from a 50-fold increase in allosteric site affinity as compared with BQCA, while re

61 This allosteric site allows for selective inhibition with res

62 rovides new insights into the mechanisms and allosteric sites [alpha(-)-interface containing] by whic

63 Surprisingly, mutations in the allosteric site also abolished the voltage-dependence of

64 cribed to stem from sole interaction with an allosteric site, although the evidence for such a mechan

65 unexpected presence of a previously unknown allosteric site and an unusual dodecameric structure for

66 sight into dNTP promiscuity at the secondary allosteric site and how enzymatic activity is modulated.

67 f a FRET-based sensor of conformation at the allosteric site and in pharmacological assays involving

68 ne domain plays a dual role in occluding the allosteric site and in stabilizing the autoinhibitory co

69 es on the reciprocal interactions between an allosteric site and its coupled orthosteric site.

70 losteric transition, we vary the size of the allosteric site and its interactions to construct a seri

71 ropose that Plasmodium Kinesin-5 shares this allosteric site and likewise can be targeted to uncover

72 of structural information regarding the A1AR allosteric site and mechanisms governing cooperativity w

73 not exhibit sodium ion binding to the D2.50 allosteric site and samples a significantly larger confo

74 ctively inhibit mutant IDH1 by binding to an allosteric site and that inhibition is competitive with

75 eps: binding of the monovalent cation to its allosteric site and transduction of this event into enha

76 ng inhibitors that when tethered revealed an allosteric site and trapped a conformation similar to th

77 3) pharmacological compounds targeting this allosteric site and utilizing this mode of inhibition.

78 of a protein provides valuable insights into allosteric sites and communication pathways.

79 rk corresponding to biologically significant allosteric sites and pathways.

80 ignificantly enhances protein motions in the allosteric sites and reduces the stability of the alpha5

81 at there are many as yet undiscovered hidden allosteric sites and suggests our methodology can identi

82 lin domain, conducted the cross-talk between allosteric sites and the mannoside-binding pocket.

83 efined orthosteric binding site and possible allosteric sites and to analyze the importance of the li

84 the experimental approaches designed to map allosteric sites and transitions.

85 concomitant association with orthosteric and allosteric sites) and that the compound displays biased

86 Affinities of isoflurane and propofol to the allosteric site are also calculated, and shown to be 3 m

87 hibition is cooperative, indicating that the allosteric sites are not independent despite being locat

88 nowledge of the target site, but most hidden allosteric sites are only discovered when a small molecu

89 The results identify the location of the allosteric site as the interface of the regulatory domai

90 that incorporates competition for active and allosteric sites, as well as functional sequestration of

91 us, the current study identifies novel c-Src allosteric sites associated with imatinib binding and ki

92 e D3R(Dopa) model are predicted to occupy an allosteric site at the extracellular extension of the po

93 op that hinders methoctramine binding to the allosteric site at this receptor subtype.

94 acting with both the orthosteric site and an allosteric site, at the M(1) mAChR.

95 Here, we review algorithms that predict allosteric sites based on sequence data and molecular dy

96 structures revealed 3 binds to an unexpected allosteric site between the C-terminal and the N-termina

97 om the orthosteric site up toward a putative allosteric site bordered by TM2, TM3, and TM7, thus cons

98 he modulator's high binding affinity for the allosteric site but not for generating allostery on the

99 of selective affinity for a poorly conserved allosteric site but rather is due to subtype-selective c

100 ctive protein kinase inhibitors by targeting allosteric sites, but these sites often involve protein-

101 However, interaction with an allosteric site by an antagonist fragment of TBPB was de

102 lactam antibiotics, wherein occupancy at the allosteric site by one facilitates occupancy by a second

103 Kinetic analysis reveals co-occupancy of the allosteric sites by TIH and L-histidine.

104 y result in a higher affinity of dTTP to the allosteric site conferring its inhibitory activity.

105 The sodium ion site is an allosteric site conserved among many G protein-coupled r

106 In addition, we discuss how targeting of allosteric sites could provide new opportunities to inhi

107 l structure shows that the compound binds an allosteric site created by the displacement of the regul

108 Upon binding a metal ion in the allosteric site, CueR switches into an activator conform

109 with PBP2a-each with the beta-lactam in the allosteric site-defined (with preceding PBP2a structures

110 antagonists bind to CCR4 at an intracellular allosteric site denoted site II.

111 Compounds targeting the allosteric site display a variety of functional activity

112 The basis for this discrimination is an allosteric site, distal from the active site, that when

113 Unexpectedly, these drugs all bind to an allosteric site distinct from the ATP-binding pocket in

114 two inhibitor molecules interact within the allosteric site, driving significant conformational chan

115 Based on increasing interest in allosteric-site drug discovery, we foresee many potentia

116 m in adult liver, harbors two noninteracting allosteric sites, each of which binds a different molecu

117 gest that tiotropium binds transiently to an allosteric site en route to the binding pocket of both r

118 of the cytosolic region was found to have an allosteric site for acyl-CoA/CoA.

119 binding site, SERT possesses a low affinity allosteric site for antidepressants.

120 Here we identified this allosteric site for efavirenz on CYP46A1 by using a comb

121 steric-binding pocket and is not part of the allosteric site for gallamine.

122 The location of the allosteric site for phenylalanine has not been establish

123 et that has previously been identified as an allosteric site for RGS regulation by acidic phospholipi

124 noceptor (alpha(1B)-AR) to help identify the allosteric site for rho-conopeptide TIA, an inverse agon

125 We show that the H3 pocket is an allosteric site for the regulation of the rotation of th

126 ntrinsic flexibility of RT can provide novel allosteric sites for inhibition.

127 cleos(t)ide analogues as well as a number of allosteric sites for nonnucleos(t)ide inhibitors.

128 ol of protein allostery and design of unique allosteric sites for potential enzyme inhibitors with re

129 ng configuration as S-trityl analogues at an allosteric site formed by loop L5 of Eg5.

130 Rather, we propose that a transient propofol allosteric site forms when the motor head binds to the m

131 s of decoupling the identification of hidden allosteric sites from the discovery of drugs that bind t

132 stinct from the orthosteric sites, so-called allosteric sites, has heralded novel approaches and mode

133 Several allosteric sites have been identified within the family

134 has a degenerate active site but a conserved allosteric site (I-site), which we show binds c-di-GMP w

135 -domain shows that they bind to the I-domain allosteric site (IDAS), the binding site of other allost

136 Binding to the allosteric site impedes dissociation of antidepressants

137 Furthermore, the presence of a novel allosteric site in an intermediate state of c-src that c

138 ng mode involving residues within TM10 in an allosteric site in BGT1 that corresponds to the alloster

139 ransfer (CEST) interactions with the primary allosteric site in bla give rise to a unique saturation

140 of a cytohesin-3 construct encompassing the allosteric site in complex with the head group of phosph

141 1,013 compounds for possible binding to the allosteric site in farnesyl diphosphate synthase (FPPS).

142 We further identify the location of an allosteric site in the complex as residing at the periph

143 he active conformation, whereas targeting an allosteric site in the dimer interface selects an inacti

144 rlaps with the previously described "common" allosteric site in the extracellular vestibule of the M1

145 Molecules targeting the allosteric site in the inactive kinase conformation (typ

146 ies, suggests that the inhibitor binds at an allosteric site in the motor protein.

147 complex show that (S)-CPP binds to a unique allosteric site in the N-terminal domain, triggering hel

148 wo small molecule inhibitors that bind to an allosteric site in the proximity of the CHK1 substrate s

149 ligand binding to the prototypical MPEP/FPEB allosteric site in vitro.

150 rotein backbone is used to identify putative allosteric sites in a series of antibody fragments.

151 ion of these methods reveals multiple hidden allosteric sites in an important antibiotic target--TEM-

152 binding site in the 5-HT(3)R corresponds to allosteric sites in anionic Cys-loop receptors, which em

153 t interest in identifying and characterizing allosteric sites in enzymes such as protein kinases both

154 ontrast to classic allostery, the active and allosteric sites in hUGDH are present as a single, bifun

155 Drug-like molecules targeting allosteric sites in proteins are of great therapeutic in

156 es a simple method for identification of new allosteric sites in PTP, and given the basis of this met

157 some GPCRs possess more than one targetable allosteric site, in addition to a growing list of putati

158 this loop motion to effector binding at the allosteric site, in order to identify the signaling path

159 yme toward 2-AG, whereas 2-AG binding at the allosteric site increases COX-2's efficiency toward AA.

160 stent with Na(+) binding to an extracellular allosteric site, independent of the ion transport sites,

161 to the conclusion that these small molecule allosteric site inhibitors of CHK1 are reversible and ar

162 e example of this, we have introduced distal allosteric sites into molecular beacons, which are optic

163 is experiments indicate that BQCA acts at an allosteric site involving residues Y179 and W400.

164 sults are also unique in suggesting that the allosteric site is also involved in controlling the volt

165 The affinity of NMS at the allosteric site is in the micromolar range for all recep

166 We report that the gamma-secretase allosteric site is located within the gamma-secretase co

167 y inhibit RNA synthesis, suggesting that the allosteric site is not always available during RNA synth

168 When this allosteric site is occupied, a multiresidue conformation

169 of MK by Mev.pp analogues revealed that the allosteric site is selective for compact, electron-rich

170 One allosteric site is surface-exposed and is located near t

171 ystal structures, a possible location of the allosteric site is within a cleft between the alpha- and

172 straightforward approach to identify hidden allosteric sites is demonstrated in protein tyrosine pho

173 Therefore, targeting allosteric sites is gaining increasing recognition as a

174 mimics of the acyl-D-Ala-D-Ala moiety at the allosteric site, lead to a conformational change, across

175 eptors suggest that TCN-201 binds to a novel allosteric site located at the dimer interface between t

176 Previously, a model was created for an allosteric site located between the flap and the core of

177 We extended previous observations that an allosteric site located in the vestibule of the receptor

178 involved variation at positions primarily at allosteric sites located outside of binding pockets, whe

179 these observations, we propose that cryptic allosteric sites may be even more ubiquitous than previo

180 ric sites, the force of anaesthetics through allosteric sites may not propagate to the neighbouring s

181 also influence the activity by binding to an allosteric site, most likely the pseudosymmetric site lo

182 ggable pocket that is the most intracellular allosteric site observed in class A G-protein-coupled re

183 ne the first view of a polyamine bound in an allosteric site of an N-acetyltransferase.

184 0.25), whereas the MA fragment binds to the allosteric site of beta(2)AR (pEC(50,diss) = 3.60 +/- 0.

185 ompetitive interaction of AA and 2-AG at the allosteric site of COX-2 might result in differential re

186 a revealed that binding of AA or 2-AG at the allosteric site of COX-2 results in a decreased catalyti

187 ity in the dimer interface could serve as an allosteric site of hTS to regulate the conformational sw

188 ovel binding mode in a previously identified allosteric site of IDH1 (R132H).

189 bostyril-linker fragment (BA-L) binds to the allosteric site of mAChR (M(2) pEC(50,diss) = 5.06 +/- 0

190 the glutamatergic neurotransmission (glycine allosteric site of NMDA receptors or alpha-amino-3-hydro

191 ocument that this mimicry exists also at the allosteric site of PBP2a of methicillin-resistant Staphy

192 ovalent metal ions indirectly influences the allosteric site of Pfk-2 by increasing its affinity for

193 OS, bringing GDP-Ras to the proximity of the allosteric site of SOS.

194 weakly binds to the catalytic but not to the allosteric site of Sos.

195 drogens of human androgen receptor (AR), the allosteric site of the AR binding function 3 (BF3) was i

196 tion mutagenesis in a single position of the allosteric site of the dimer interface, and we show that

197 ts as an allosteric ligand that binds at the allosteric site of the enzyme's interfacial surface, shi

198 (11)C-ABP688, a PET ligand that binds to an allosteric site of the metabotropic glutamate 5 receptor

199 o affinity of (11)C-ABP688 for binding to an allosteric site of the metabotropic glutamate 5 receptor

200 ompounds showed type III interaction with an allosteric site of the proteins.

201 ility of structure-based screening targeting allosteric sites of GPCRs.

202 o provides an effective approach to identify allosteric sites of multimeric enzymes in general.

203 several EPAC1 mutants that target different allosteric sites of the cAMP-binding domain (CBD).

204 t form a network linking the orthosteric and allosteric sites of the M4 receptor, which provides new

205 al approach to discover a previously unknown allosteric site on 15-LOX.

206 llosteric sites on kinases and that a single allosteric site on a protein kinase can be exploited for

207 eric site on mAChR and a novel extracellular allosteric site on beta(2)AR, respectively, were detecte

208 complex shows that NSC13345 binds to a novel allosteric site on cathepsin K.

209 es, we find that Org 27569 binds to a unique allosteric site on CB1 and show that it can act alone (w

210 G may recognize more than one anion-binding, allosteric site on FXIa.

211 ligands and describes another example of an allosteric site on G-protein-coupled receptors that can

212 GPCR activation of GRKs involves an allosteric site on GRKs distinct from the catalytic site

213 Interactions of THRX-198321 with an allosteric site on mAChR and a novel extracellular allos

214 work has extensively characterized a common allosteric site on mGlu5, termed the MPEP (2-Methyl-6-(p

215 ydroxyb enzamide), interacts with a separate allosteric site on mGlu5.

216 SOS is inactive unless Ras is bound to an allosteric site on SOS, and the Dbl homology (DH) and Pl

217 ecific drug-protein interactions validate an allosteric site on the channel periphery and its relevan

218 artial inverse agonist at the benzodiazepine allosteric site on the gamma-aminobutyric acid (GABA)(A)

219 dies suggest that activated GPCRs dock to an allosteric site on the GRKs and thereby stimulate kinase

220 However, LY2119620 also binds to an allosteric site on the human M2 subtype.

221 structure to LY2033298 and binds to the same allosteric site on the human M4 mAChRs.

222 proach to targeting a functionally conserved allosteric site on the protein kinase PDK1 called the PD

223 AChR), but it possesses low affinity for the allosteric site on the receptor.

224 rrogate structurally and functionally how an allosteric site on the Ser/Thr kinase, 3-phosphoinositid

225 induced by ligand binding to orthosteric or allosteric sites on a G protein-coupled receptor (GPCR)

226 These data suggest the orthosteric and allosteric sites on CHRMs respond differently to divalen

227 ulfide trapping is useful for characterizing allosteric sites on kinases and that a single allosteric

228 To probe directly the allosteric sites on M2 and M4, we radiolabeled LY2119620

229 took advantage of the many tools for probing allosteric sites on mGlu(5) to validate an operational m

230 hanced receptor gating by rapidly binding to allosteric sites on open channels, initiating a rate-lim

231 Computational methods to predict allosteric sites on proteins are needed to aid the disco

232 mal modes can enhance our ability to predict allosteric sites on proteins.

233 nslational modifications or metal binding to allosteric sites on the enzyme, which stabilize inactive

234 There are at least four distinct allosteric sites on the polymerase to which several smal

235 has spurred current research into targeting allosteric sites on these receptors.

236 At this allosteric site, ORG27569 promotes an intermediate confo

237 Assays showed that NIT acts on an allosteric site other than the dimerization interface.

238 Two other RNA molecules bind DNMT3A at an allosteric site outside the catalytic domain, causing no

239 Antagonist AZ3451 binds to a remote allosteric site outside the helical bundle.

240 gonist MK-0893, which is found to bind to an allosteric site outside the seven transmembrane (7TM) he

241 ion of these residues is consistent with two allosteric sites per dimer, with each site containing re

242 f rhodopsin-like GPCRs suggest that specific allosteric sites play a pivotal role in activating struc

243 s with mGlu5 modulators have relied on mGlu5 allosteric site positron emission tomography (PET) radio

244 et regions in LF did not, suggesting that an allosteric site preferentially recognizes alpha-helical

245 Competition of AM-8138 for the allosteric site prevented the inhibition of COX-2-depend

246 Allosteric sites provide targets to fine-tune protein ac

247 by the effector, modeled by the size of the allosteric site, provides an order parameter for alloste

248 cy may result from binding of propofol to an allosteric site recently identified by X-ray crystallogr

249 Finding small molecules that target allosteric sites remains a grand challenge for ligand di

250 ic potential of these pockets--called hidden allosteric sites--remains unclear.

251 nt points: 1) the modification of the Cys148 allosteric site results in significant changes to the RG

252 ee-dimensional NMR solution structure of the allosteric site revealed an alpha-helix with a loop conn

253 ing an additional retinoid(s) at a separate, allosteric site(s).

254 This model revealed that the active and allosteric sites show cooperativity.

255 Calcium acetate binding in the allosteric site stabilizes the conformation observed in

256 Occupancy of the allosteric site sterically hinders ligand unbinding from

257 nds specifically and reversibly to an mGluR2 allosteric site, strongly suggesting that it is a promis

258 th anesthetics have a lower affinity for the allosteric site than for the unoccupied pore.

259 were more significantly enriched at protein allosteric sites than tolerated mutations, suggesting a

260 in a protein involves effector binding at an allosteric site that changes the structure and/or dynami

261 However, 1 also bound an alternate/allosteric site that could alternatively account for the

262 GTP relieves autoinhibition by binding to an allosteric site that includes the autoinhibitory element

263 thway connecting the active site to the main allosteric site that remains in the substrate-bound form

264 Instead, omecamtiv mecarbil binds to an allosteric site that stabilizes the lever arm in a prime

265 xperiments, the human Kinesin-5 has multiple allosteric sites that are "druggable." One site in parti

266 p38gamma contains allosteric sites that are conserved among eukaryotic kin

267 of BCR-ABL have led to the identification of allosteric sites that are distant from the ATP-binding p

268 erefore, have shifted to the exploitation of allosteric sites that can regulate phosphatase activity

269 t propofol is not binding at the ATP site or allosteric sites that modulate microtubule-activated ATP

270 r, DF2593A, guided by the hypothesis that an allosteric site, the "minor pocket," previously characte

271 This process is tightly regulated via two allosteric sites, the specificity site (s-site) and the

272 Here, the first structure of an NSAID allosteric site-the MEF-binding site of SULT1A1-is deter

273 anism by binding to a previously undescribed allosteric site: the interface of the two cAMP binding d

274 is class of inhibitors acts by binding to an allosteric site; the novel binding site is proposed in s

275 n that couples effector ligand binding at an allosteric site to a structural and/or dynamic change at

276 ta2 loop, and beta4 loop and functions as an allosteric site to enhance the efficiency of activation

277 competes with NEA at a novel, extracellular, allosteric site to enhance the maximal stimulation evoke

278 rectly activate the receptor, but acts at an allosteric site to increase functional responses to orth

279 ent pathways of signal transduction from the allosteric site to the active site.

280 ay for the signal transmission from the P450 allosteric site to the active site.

281 In particular, the transmission from the allosteric site to the signaling surface of the receiver

282 strate the utility of purposefully targeting allosteric sites to obtain mutant-selective inhibitors.

283 samples across 33 cancer types into protein allosteric sites to prioritize the mutated allosteric pr

284 y nucleic acid binding affinity; and surface allosteric sites, to control recruitment of additional p

285 rtant insights into the structural bases for allosteric site-to-active site communication and for bet

286 Cryptic allosteric sites--transient pockets in a folded protein

287 us influenzae beta-carbonic anhydrase (HICA) allosteric site variants V47A and G41A were overexpresse

288 tion of hubs preferentially connected to the allosteric site was found in close agreement with key re

289 mGluR5 receptor binding specifically to an allosteric site was observed by using positron emission

290 To target this putative allosteric site, we generated and characterized fragment

291 The two allosteric sites were shown to regulate the binding of a

292 increase in affinity for the peptide in the allosteric site when KIX is prebound by a complementary

293 of the cube also provided a distinct second allosteric site, where the binding of tetraphenylborate

294 opentenyl diphosphate, IPP) site, not to the allosteric site, whereas zoledronate bound via Mg(2+) to

295 tions allows us to identify potential hidden allosteric sites, which we then test via thiol labeling

296 radiolabeled [11C]ABP688, which binds to an allosteric site with high specificity.

297 however, has been on modes of targeting GPCR allosteric sites with chemical probes due to the potenti

298 dies indicate that dGTP binding to the first allosteric site, with nanomolar affinity, is a prerequis

299 y of discovery programs focused on targeting allosteric sites, with allosteric ligands now available

300 to be expected that binding of Na(+) to the allosteric site would be nearly saturated.