ライフサイエンスコーパス: alpha-amanitin

1 bolish the effect with low concentrations of alpha-amanitin.

2 of resuming transcription in the presence of alpha-amanitin.

3 ndent of transcription as it is resistant to alpha-amanitin.

4 of RNA polymerase II, the subunit that binds alpha-amanitin.

5 radation of endogenous Pol II using a toxin, alpha-amanitin.

6 lity, as does inhibition of transcription by alpha-amanitin.

7 ctional chromatin insulators are affected by alpha-amanitin.

8 ly diminished by treatment of the cells with alpha-amanitin.

9 complex, which is specifically inhibited by alpha-amanitin.

10 aged in active transcription and arrested by alpha-amanitin.

11 is selectively resistant to inhibition with alpha-amanitin.

12 ar extracts with the transcription inhibitor alpha-amanitin.

13 ranscriptional inhibitors, actinomycin D and alpha-amanitin.

14 nt to high concentrations (100 microg/ml) of alpha-amanitin.

15 -beta-D-ribofuranosylbenzimidazole (DRB) and alpha-amanitin.

16 thesis inhibitors actinomycin D, DRB, H7 and alpha-amanitin.

17 with inhibition of ongoing transcription by alpha-amanitin.

18 e II transcription was globally inhibited by alpha-amanitin.

19 s resistant to the transcriptional inhibitor alpha-amanitin.

20 tein-coding genes is relatively resistant to alpha-amanitin (50% inhibition = 250 microg alpha-amanit

21 rase that is resistant to the mushroom toxin alpha-amanitin, a characteristic of transcription by RNA

22 d centriole overduplication are abrogated by alpha-amanitin, a potent and specific RNA pol II inhibit

23 of RNA pol II dephosphorylation confirmed by alpha-amanitin, a specific RNA pol II inihibitor, showin

24 In the presence of the translocation blocker alpha-amanitin, a steady state condition is established

25 alpha-Amanitin, a transcription inhibitor, did not suppr

26 Inhibition of zygotic transcription with alpha-amanitin also induced apoptosis.

27 nation of Pol II is significantly induced by alpha-amanitin, an amatoxin that blocks Pol II elongatio

28 bisporigera, AMA1 and PHA1, directly encode alpha-amanitin, an amatoxin, and the related bicyclic he

29 delivery of a polar cell-impermeable toxin, alpha-amanitin, an inhibitor of RNA polymerase II.

30 ymerase II (RNAPII) translocation inhibitors alpha amanitin and 5,6-dichloro-1-beta-D-ribobenzimidazo

31 ptotic hepatocytes were also observed in the alpha-amanitin and acetaminophen-induced liver injury mo

32 We here show that transcriptional inhibitors alpha-amanitin and actinomycin D specifically disrupt th

33 nitiation factor eIF-4C that is inhibited by alpha-amanitin and correlated with a transient increase

34 ivity of G. lamblia RNAP II transcription to alpha-amanitin and found that unlike most other eukaryot

35 as compared with 8-cell embryos treated with alpha-amanitin and MII.

36 In Amanita bisporigera, alpha-amanitin and phallacidin are synthesized as 35- an

37 alpha-Amanitin and phallacidin are synthesized as propro

38 transcriptional inhibitors actinomycin D and alpha-amanitin and requires the kinase activity of ATM b

39 lpha-amanitin but sensitive to 100 microg/ml alpha-amanitin and tagetitoxin, suggesting involvement o

40 ymerase II that is relatively insensitive to alpha-amanitin and that differs from typical eukaryotic

41 V require an RNA primer, are insensitive to alpha-amanitin, and differ in their ability to displace

42 NA-dependent RNA polymerases (actinomycin D, alpha-amanitin, and rifampin).

43 eta colocalized with RNA polymerase II in an alpha-amanitin- and actinomycin D-sensitive manner.

44 nucleoside triphosphate (NTP) substrates and alpha-amanitin are added to a human RNA polymerase II el

45 Using alpha-amanitin as a dynamic probe of the RNA polymerase

46 s found to correlate with the sensitivity to alpha-amanitin, as S. pombe was intermediate between hum

47 of genomic HDV RNA was totally inhibited by alpha-amanitin at concentrations as low as 2.5 microg/ml

48 However, we found that alpha-amanitin-based antibody-drug conjugates are highly

49 blocked by co-incubation with picrotoxin or alpha-amanitin but is insensitive to nifedipine, indicat

50 resistant to the RNA polymease II inhibitor alpha-amanitin but is sensitive to short interfering RNA

51 ption of TARE-6 was resistant to 1 microg/ml alpha-amanitin but sensitive to 100 microg/ml alpha-aman

52 Here we show that low doses of alpha-amanitin-conjugated anti-epithelial cell adhesion

53 ynthesized by an RNA polymerase resistant to alpha-amanitin, consistent with previously published rep

54 ion by gamma interferon or its inhibition by alpha-amanitin did not alter nucleosome occupancy, posit

55 ition of pol I by cycloheximide or pol II by alpha-amanitin did not significantly affect the PNC.

56 olymerase II in a complex with the inhibitor alpha-amanitin has been determined by x-ray crystallogra

57 Previous clinical applications of alpha-amanitin have been limited owing to its liver toxi

58 Transcription inhibition by alpha-amanitin in vitro enhanced pol II ubiquitylation a

59 200 micrograms/ml but not 1 microgram/ml of alpha-amanitin indicates transcription of the mouse YRNA

60 cifically renders Pol II highly resistant to alpha-amanitin, indicating a functional interaction betw

61 nscription of the GP63 genes is sensitive to alpha-amanitin, indicating that they are transcribed by

62 Transcription of the ESAG-Is is sensitive to alpha-amanitin, indicating that they are transcribed by

63 as unaffected by either kinase inhibitors or alpha-amanitin-induced depletion of pol II.

64 ng their sensitivity to DRB, indicating that alpha-amanitin induces apoptosis solely by inhibiting RN

65 Additionally, both actinomycin D and alpha-amanitin inhibited the increase in uPA mRNA by PAF

66 We propose that alpha-amanitin-inhibited Pol II elongation, which is slo

67 We show by alpha-amanitin inhibition that RNA polymerase II (RNAPII

68 active in the following bond addition cycle, alpha-amanitin inhibits elongation at each translocation

69 alpha-Amanitin insensitivity confirmed that overexpressi

70 d substrate selectivity, results from direct alpha-amanitin interference with the TL.

71 Although alpha-amanitin interferes with the transcript cleavage s

72 We show that pHLIP can deliver alpha-amanitin into cells in a pH-dependent fashion and

73 The toxin alpha-amanitin is frequently employed to completely bloc

74 e sensitivity to different concentrations of alpha-amanitin is that expected for human RNA polymerase

75 loro-1-beta-D-ribofuranosyl-benzimadazole or alpha-amanitin leads to accumulation of cellular p53 pro

76 tion was inhibited by a low concentration of alpha-amanitin (<3 microgram/ml) and could be partially

77 alpha-amanitin (50% inhibition = 250 microg alpha-amanitin/ml).

78 in the presence of the translocation blocker alpha-amanitin, NTPs (but not deoxynucleotide triphospha

79 yzing the effect of the fungus-derived toxin alpha-amanitin on the transcription of protein-coding ge

80 Treatment of cells with alpha-amanitin or actinomycin D revealed that extension

81 ect was blocked by cycloheximide, but not by alpha-amanitin or actinomycin D.

82 elongating Pol II-DNA complexes arrested by alpha-amanitin or cisplatin lesions, triggering ubiquiti

83 Suppression of POLR2A with alpha-amanitin or small interfering RNAs selectively inh

84 Inhibition of transcription using alpha-amanitin, or the dissolution of R loops by transie

85 ion complexes were slowed by the presence of alpha-amanitin, origin activity depended on the orientat

86 vating model and models of acetaminophen and alpha-amanitin poisoning were used.

87 itin that is supported by rerefinement of an alpha-amanitin-Pol II crystal structure.

88 Inhibition of Pol II activity using alpha-amanitin reduced expression of a number of Pol III

89 his study we carry out a genetic analysis of alpha-amanitin resistance in a population sample of Dros

90 Engineered expression of an alpha-amanitin resistance RNAP II gene rendered cells re

91 m in a multidrug resistance gene (Mdr65A) in alpha-amanitin resistance.

92 The antigenomic RNA labeling was alpha-amanitin resistant and localized to the nucleolus.

93 mutants contribute to the difference between alpha-amanitin-resistant and alpha-amanitin-sensitive th

94 am/ml) and could be partially restored by an alpha-amanitin-resistant mutant pol II; however, surpris

95 Using an alpha-amanitin-resistant polymerase, we provide evidence

96 Utilizing alpha-amanitin-resistant RNA polymerase II mutants with

97 Using plasmid constructs that express an alpha-amanitin-resistant RNAP II subunit with a truncate

98 d human immunodeficiency virus 1, we used an alpha-amanitin-resistant system developed previously.

99 on units, and inhibition of transcription by alpha-amanitin resulted in the initiation of replication

100 injected with the transcriptional inhibitor, alpha-amanitin, revealed that the symmetry of cell divis

101 The genomic RNA labeling was alpha-amanitin sensitive and more diffusely localized in

102 the 4.3 and 4.4 loci) whose transcription is alpha-amanitin sensitive.

103 nd confirmed that HDV RNA synthesis had both alpha-amanitin-sensitive and -resistant components.

104 Based on alpha-amanitin-sensitive BrUTP incorporation, transcript

105 se GeneChip set, we characterized the set of alpha-amanitin-sensitive genes expressed during the 1- a

106 ference between alpha-amanitin-resistant and alpha-amanitin-sensitive third chromosome lines, the und

107 e hsp70 intergenic region promoter can drive alpha-amanitin-sensitive transcription at an internal po

108 tsp, was functionally sufficient to support alpha-amanitin-sensitive transcription.

109 pts present on the MOE430 GeneChip set to be alpha-amanitin-sensitive.

110 of genes expressed in the 2-cell embryo are alpha-amanitin-sensitive.

111 A comparison of the alpha-amanitin sensitivity of transcription in naturally

112 ion is carried out by an RNA polymerase with alpha-amanitin sensitivity reminiscent of SL RNA synthes

113 ic transcription began, but experiments with alpha-amanitin show that cyclin E degradation is not dep

114 of the 50-amino-acid region thought to bind alpha-amanitin shows that this region of the trichomonad

115 eatment of cells with actinomycin D, DRB, or alpha-amanitin, specific inhibitors of Pol II, disperses

116 e show that inhibition of RNAPII activity by alpha-amanitin specifically blocks processing of rRNA.

117 Transcription in CS-B cells is sensitive to alpha-amanitin, suggesting that it is RNA polymerase II-

118 urs following activation but is sensitive to alpha-amanitin, suggesting that polymerase movement is n

119 ribonucleotides into RNA in the presence of alpha-amanitin, suggesting that the polymerase I enzyme

120 and HDV RNAs have different sensitivities to alpha-amanitin, suggesting that these two strands are sy

121 he RNA polymerase inhibitors tagetitoxin and alpha-amanitin that are consistent with RNA polymerase I

122 a functional interaction between His1085 and alpha-amanitin that is supported by rerefinement of an a

123 red to the two-cell stage in the presence of alpha-amanitin, this change in transcript abundance is n

124 (1.7-kb) antigenomic RNA was not affected by alpha-amanitin to a concentration higher than 25 microgr

125 nous insulin receptors in cells treated with alpha-amanitin to block host cell mRNA synthesis.

126 istribution described nonspecific binding of alpha-amanitin to yeast RNA polymerase II.

127 o inhibit RNA polymerases I, II, and III) or alpha-amanitin (to inhibit RNA polymerases II and III) a

128 eptides outside the plant kingdom (e.g., the alpha-amanitin toxin gene family in the mushroom, Amanit

129 s as 'chemical mutation' tools coupling with alpha-amanitin transcription inhibition assay to systema

130 ntriole duplication proceeded undisturbed in alpha-amanitin-treated cells.

131 alpha-Amanitin treatment had no effect on the ratio of p

132 ly single- and triple-knockdown experiments, alpha-amanitin treatment, transcriptome profiling and ch

133 Apoptosis induced by cycloheximide or alpha-amanitin was blocked by injection of RNA encoding

134 Following treatment with alpha amanitin we observed a profound reduction in the o

135 The addition of alpha-amanitin, which can inhibit transcription, reduced

136 -beta-D-ribofuranosylbenzimidazole (DRB) and alpha-amanitin, which inhibit RNAP II function by two di

137 We find that the mushroom toxin alpha-amanitin, which inhibits TL mobility, suppresses t

138 PADT is inhibited by alpha-amanitin, which presumably blocks the required con

139 cells resistant to induction of apoptosis by alpha-amanitin without affecting their sensitivity to DR