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1 too large to assay and to interact with the alpha-amylase.
2 sis, different from the case of thermostable alpha-amylase.
3 ion, in addition to their ability to inhibit alpha-amylase.
4 nificantly improved secretion of recombinant alpha-amylase.
5 enital fluids that correlated with levels of alpha-amylase.
6 ead flour after optimisation by additions of alpha-amylase.
7 dextrins, and glycogen treated with salivary alpha-amylase.
8 hibited higher inhibitory activities against alpha-amylase.
9 with mucosal alpha-glucosidases and not just alpha-amylase.
10 inhibitors in complex with human pancreatic alpha-amylase.
11 on of the S. gordonii gene amyB, encoding an alpha-amylase.
12 dependent on the presence of human salivary alpha-amylase.
13 -glucans digested by purified human salivary alpha-amylase.
14 P4 and CSP6) showed high affinity in binding alpha-amylase.
15 ng and hydrolytic activity of human salivary alpha-amylase.
16 ents synthesis of secretory proteins such as alpha-amylase.
17 ed by transglycosylation using a recombinant alpha-amylase.
18 lin-induced and ABA-suppressed expression of alpha-amylase.
19 Ca(2+) and Zn(2+) metal ions in P. furiosus alpha-amylase.
20 C430) are conserved in the P. kodakaraensis alpha-amylase.
21 C165S dramatically destabilized P. furiosus alpha-amylase.
22 t but also necessary for the GA induction of alpha-amylase.
23 uate into a point-of-care detection tool for alpha-amylase.
24 malian digestive enzymes, namely trypsin and alpha-amylase.
25 d was baked with and without the addition of alpha-amylase.
26 baker was sensitized also to either flour or alpha-amylase.
27 similar to most family 10 xylanases and the alpha-amylases.
29 ergistic action of beta-amylase 1 (BAM1) and alpha-amylase 3 (AMY3)-enzymes that are normally not req
30 xtracts inhibited alpha-glucosidase (37.8%), alpha-amylase (35.6%), dipeptidyl peptidase-IV (34.4%),
31 he present study investigates the effects of alpha-amylase (6 and 10ppm), xylanase (70 and 120ppm) an
33 an inhibitor to inactivate human pancreatic alpha-amylase, a therapeutic target for oral hypoglycemi
34 of stress loading, chromogranin A (CgA) and alpha-amylase (AA) are supposed to link the activity of
35 PhytoPs) on four enzymes: alpha-glucosidase, alpha-amylase, acetylcholinesterase, and butyrylcholines
36 for which possible binding modes within the alpha-amylase active site could be investigated in silic
41 otein and negatively with monomeric protein, alpha-amylase activity and sodium carbonate solvent rete
42 purified protein immunoprecipitated secreted alpha-amylase activity and verified the enzymatic identi
44 imilar acceleration in the appearance of the alpha-amylase activity in deembryonated transgenic grain
47 This work provides new insights into how alpha-amylase activity may be regulated in the chloropla
49 sed satisfactorily for the assessment of the alpha-amylase activity over activity range (3-321U/L) in
52 in the rate of germination and appearance of alpha-amylase activity with a 1.6- to 2.8-fold increase
53 seaweeds appeared to be potent inhibitors of alpha-amylase activity with an IC50 of (0.075+/-0.010-0.
60 plemented with additional alpha-amylase, but alpha-amylase added to weak flour can lead to decreased
62 , myricetin showed the highest inhibition of alpha-amylase, alpha-glucosidase and lipase (IC50: 0.38m
63 wards metabolic syndrome-associated enzymes (alpha-amylase, alpha-glucosidase and lipase) was evaluat
64 ssays, all PSP samples inhibited the enzymes alpha-amylase, alpha-glucosidase and xanthine oxidase.
65 ssociated with metabolic syndrome, including alpha-amylase, alpha-glucosidase, lipase and hydroxyl me
66 erol absorption via their ability to inhibit alpha-amylase, alpha-glucosidase, sodium-glucose transpo
67 that the cell wall anchored starch-degrading alpha-amylase, Amy13K of E. rectale harbors five CBMs th
69 obus solfataricus secretes an acid-resistant alpha-amylase (amyA) during growth on starch as the sole
73 lth-promoting benefits (anticancer activity, alpha-amylase and alpha-glucosidase inhibition, angioten
74 rch was to evaluate extracts of seaweeds for alpha-amylase and alpha-glucosidase inhibitory effects.
75 llet cultivars showed superior inhibition of alpha-amylase and alpha-glucosidase than foxtail millet
76 activities and inhibitory properties against alpha-amylase and alpha-glucosidase were investigated.
83 s casei rhamnosus) and tri-enzyme (protease, alpha-amylase and chicken pancreas conjugase) extraction
85 ed as an index of fear arousal, and salivary alpha-amylase and cortisol concentrations were assayed a
86 viewed in a sequential manner beginning with alpha-amylase and followed by alpha-glucosidase to produ
87 testinal extract containing a combination of alpha-amylase and mucosal alpha-glucosidase activities,
88 had inhibitory effects on the activities of alpha-amylase and pancreatic lipase, while they rendered
90 e results clarify the biological role of the alpha-amylase and provide additional methods for the dir
91 e in chloride affinity between the wild-type alpha-amylase and the K300R mutant, in good agreement wi
92 d on starch included the major extracellular alpha-amylase and two distinct alpha-glucanotransferases
94 ligosaccharide complex structures of various alpha-amylases and cyclodextrin glucanotransferase and r
95 enzymes for starch: salivary and pancreatic alpha-amylases and four mucosal alpha-glucosidases, incl
96 ABA prevents the transcription of genes for alpha-amylases and other secreted hydrolytic enzymes, bu
97 unction of storage time, usage of maltogenic alpha-amylases and spatial position in the loaf by textu
98 th four carbohydrases viscozyme, celluclast, alpha-amylase, and amyloglucosidase, and then extracted
99 pancreatic extract containing predominantly alpha-amylase, and intestinal extract containing a combi
100 starch hydrolysis (e.g. cell wall invertase, alpha-amylase, and starch phosphorylase) were expressed
104 203A/Y276A/W284A/W316A/W388A; human salivary alpha-amylase aromatic residue multiple mutant (HSAmy-ar
106 For this purpose, alpha-glucosidase and alpha-amylase assays were assessed; among all bean ecoty
108 ubjected to hydrolysis by porcine pancreatic alpha-amylase at 37 degrees C for several digestion time
111 sp197, Glu233, His299, Asp300 and His305 for alpha-amylase; (b) His353, Ala354, His383, Glu384, His38
112 ctivities of the germination-related enzymes alpha-amylase, beta-amylase and beta-glucanase varied by
115 rmostable variants of Bacillus licheniformis alpha-amylase (BLA) that result from the incorporation o
117 ket bakers are exposed not only to flour and alpha-amylase but also to other 'improver' enzymes, the
118 n acinar cell cytoplasm and colocalizes with alpha-amylase but is not detected in pancreatic islets.
119 ity needs to be supplemented with additional alpha-amylase, but alpha-amylase added to weak flour can
120 mately 58-kDa protein that was identified as alpha-amylase by Western blotting using anti-alpha-amyla
121 ling in barley, we studied the regulation of alpha-amylases by SA and a WRKY gene whose expression is
127 ns with active site residues of T. castaneum alpha-amylase compared to C. chinensis alpha-amylase, wh
128 here is a high-molecular-weight glycoprotein-alpha-amylase complex which is capable of inhibiting GTF
130 for larger systems such as human pancreatic alpha-amylase, concanavalin, Pichia pastoris lysyl oxida
132 the substrate-binding site of human salivary alpha-amylase contains two residues Trp58 and Trp59, whi
133 oteins were identified, of which five [i.e., alpha-amylase; copper zinc superoxide dismutase; protein
135 of potential functional divergences for the alpha-amylase, D-isomer-specific 2-hydroxyacid dehydroge
136 The lack of bedside monitoring devices for alpha-amylase detection has hitherto restricted the clin
138 ion of genes encoding high-isoelectric point alpha-amylase during grain development and that the LMA
141 nels, lower density kernels displayed higher alpha-amylase, endoxylanase, and peptidase activities as
142 erized for the assessment of the activity of alpha amylase enzyme in urine and serum samples for earl
146 ase, PKABA1, is known to suppress GA-induced alpha-amylase expression, PKABA1 RNAi did not hamper the
149 roduct, a novel protein with homology to the alpha-amylase family of glycosyl hydrolases, and UGP1, a
150 ion curves obtained using porcine pancreatic alpha-amylase for a range of particle size fractions.
152 the most effective (IC50: 0.25mg/mL) against alpha-amylase; Fraction V from black turtle bean was the
158 rAhAI showed differential inhibition against alpha-amylases from human, insects, fungi and bacteria.
164 essible WRKY genes inhibit the expression of alpha-amylase genes in rice, we studied the steady state
167 cross-reactivity to mealworm tropomyosin or alpha-amylase, hexamerin 1B precursor and muscle myosin,
170 show that the changes of chloride binding to alpha-amylase, human serum albumin (HSA) and Omp32 with
172 and 2885 +/- 85.4 mug/ml, respectively) and alpha-amylase (IC50 343 +/- 26.2 and 167 +/- 6.12 mug/ml
173 ractions displayed strong inhibition towards alpha-amylase [IC50, 108.68 mug/ml (bran) and 148.23 mug
174 nnatifida showed inhibitory activity against alpha-amylase, IC50 0.74+/-0.02mg/ml and 0.81+/-0.03mg/m
176 in commercially unacceptably high levels of alpha-amylase in harvest-ripe grain in the absence of ra
177 The adoption of an enzymatic treatment, with alpha-amylase in order to reduce the paste viscosity of
178 rates of sensitization to enzymes other than alpha-amylase in UK supermarket bakers; in only a small
179 agin was a very effective inhibitor of human alpha-amylase in vitro, comparable to the drug acarbose.
180 ations and conditions for the application of alpha-amylases in sugarcane processing are discussed in
181 nhibitory effect of ABA on the expression of alpha-amylase, indicating that a PKABA1-independent sign
183 ype III sorghum caused a 60-80% reduction in alpha-amylase inhibition compared to a 20% reduction by
185 ivities, inhibition of alpha-glucosidase and alpha-amylase, inhibition of angiotensin-converting enzy
186 iadin, high-molecular-weight (HMW) glutenin, alpha-amylase inhibitor (AAI) dimer, and wheat lipid tra
188 5 components, Tri a 27, Tri a 28, tetrameric alpha-amylase inhibitor CM2 (Tri a 29.02), serine protea
192 B/POZ proteins (BTB), Glutaredoxins, Trypsin alpha-amylase inhibitor proteins, and Zf-Dof proteins.
194 , which is modified from the beta-hairpin of alpha-amylase inhibitor tendamistat (residues 15-23), is
196 as to characterize the expression of various alpha-amylase inhibitors (alphaAIs), well known anti-nut
197 e was observed in total starch content (TS), alpha-amylase inhibitors activity (alphaAI) and eGI valu
199 Similar to other knottins, cystine knot alpha-amylase inhibitors are highly resistant to degrada
200 ur results expand membership of cystine knot alpha-amylase inhibitors in the Apocynaceae family and t
203 ne motif superfamily of protease inhibitors, alpha-amylase inhibitors, seed storage proteins, and lip
204 phenolic compounds and alginates are potent alpha-amylase inhibitors, thereby potentially retarding
208 (i.e., ABTS (42.2%) and FRAP (0.81 mM)) and alpha-amylase inhibitory activity (62.1%), was then subj
209 ted protein co-precipitates showed increased alpha-amylase inhibitory activity compared to non-sonica
210 thod for the detection and quantification of alpha-amylase inhibitory activity using the glucose assa
212 exhibited the highest alpha-glucosidase and alpha-amylase inhibitory activity with IC50=1.1+/-0.1mug
213 e loss of ABTS, DPPH scavenging activity and alpha-amylase inhibitory capacity, whereas the incorpora
215 cts of Ascophyllum nodosum had the strongest alpha-amylase inhibitory effect with IC50 values of 53.6
222 urther challenges the conventional view that alpha-amylase is the only rate-determining enzyme involv
226 the salivary step (pH 6.9, 5 min, 3.9 units alpha-amylase/ml), the gastric step (pH 2, 90 min, 71.2
231 cosidases can have a synergistic effect with alpha-amylase on granular starch digestion, consistent w
232 was inhibited by GIF but not by uncomplexed alpha-amylase or an unrelated high-molecular-weight glyc
235 imultaneously co-immobilizing three enzymes; alpha-amylase, pectinase and cellulase onto amino-functi
237 (ABA) and gibberellins (GA) in pre-maturity alpha-amylase (PMA) formation in developing wheat grain,
238 stion of potato starch by porcine pancreatic alpha amylase (PPAA) was investigated using isolated sta
239 K-Q-7 strain also showed a 94.1% increase in alpha-amylase production compared with NK-DeltaLP strain
240 lpha-amylase activity reveal that GA-induced alpha-amylase production in aleurone cells is inhibited
241 play a role include chloroplast development, alpha-amylase production in aleurone tissue, NO-dependen
242 idase gene driven by the GA-inducible Amy32b alpha-amylase promoter (Amy32b-GUS) and the ABA-inducibl
244 ent specifically represses GA-induced Amy32b alpha-amylase promoter but not abscisic acid-induced HVA
246 the ABA-repressible and GA-inducible Amy32b alpha-amylase promoter reporter construct (Amy32b-GUS) b
247 rge T Ag oncoprotein as a transgene from the alpha-amylase promoter, resulting in the development of
249 ptimization of trienzyme treatment combining alpha-amylase, protease and gamma-carboxy peptidase allo
250 hibitors) or even different hydrolases (e.g. alpha-amylase/protease inhibitors preventing both early
251 or the detection of immunosuppressant drugs, alpha-amylase protein, or protease activity of thrombin
255 TAKA-term and Pyrococcus kodakaraensis KOD1 alpha-amylases, respectively) even without adding Ca(2+)
256 Although Ca(2+) is known to contribute to alpha-amylase's stability, the absence of two out of the
257 nt study examined salivary CORT and salivary alpha-amylase (sAA), an indirect measure of NE, in relat
259 resistance (TER), and polarized secretion of alpha-amylase secretion after beta-adrenergic receptor s
260 ibitory effect is unlikely due to repressing alpha-amylase secretion or inhibiting alpha-amylase enzy
263 tential function of cysteines in P. furiosus alpha-amylase stability, these five residues were substi
264 milar rotation was observed in the mammalian alpha-amylase structure caused by an equivalent tryptoph
267 yrococcus furiosus produces an extracellular alpha-amylase that belongs to glycosyl hydrolases' famil
268 induces and ABA suppresses the expression of alpha-amylases that are essential for the utilization of
269 PKABA1 strongly inhibit the GA induction of alpha-amylase, they have no effect on GAMyb-transactivat
272 Starch digestion involves the breakdown by alpha-amylase to small linear and branched malto-oligosa
274 ng after starch is extensively hydrolyzed by alpha-amylase (to produce alpha-limit dextrins (alpha-LD
275 xyl radical scavenging assay, except for the alpha-amylase treated sample, all other samples demonstr
277 In this work, the allergen Tri a 30 (the CM3 alpha-amylase/trypsin inhibitor) was quantified in durum
282 es including beta-amylase, isoamylase 3, and alpha-amylase was also reduced in the myb305 plants.
284 ts to bind to bovine serum albumin (BSA) and alpha-amylase was studied by fluorescence quenching of p
285 raphy with human saliva and Tenebrio molitor alpha-amylases was used to assay inhibition activity.
288 s not inhibit the synthesis and secretion of alpha-amylase when Cl- ions are omitted from the incubat
289 aneum alpha-amylase compared to C. chinensis alpha-amylase, which could be the rationale behind the d
290 ap has shown that crystals of pig pancreatic alpha-amylase, whose structure we reported more than 15
291 le inhibitor of porcine and human pancreatic alpha-amylase with an IC(50) value of 0.026 and 0.025 mM
292 c acid was found to be a potent inhibitor of alpha-amylase with an IC50 value of 0.046+/-0.004mg/ml.
293 characterization of these gene products, one alpha-amylase with exceptional process compatibility and
294 erum albumin (HSA) and Omp32 with pH, and of alpha-amylase with mutation agree well with experimental
295 sures via the salivary analytes cortisol and alpha-amylase with self-assessments of psychosomatic str
296 arch forms were evaluated for two commercial alpha-amylases with high (HT) and intermediate (IT) temp
297 d inhibition of bacterial and human salivary alpha-amylases with IC50 values of 0.11 and 0.04mumol, r
298 microbial DNA libraries was used to identify alpha-amylases with phenotypic characteristics compatibl
299 e properties among other characterized plant alpha-amylases, with a pH optimum of 7.5-8, appropriate
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