ライフサイエンスコーパス: alpha-tubulin

1 o fluorescently label an endogenous protein (alpha-tubulin).

2 n bend that predicts a novel engagement with alpha-tubulin.

3 rgets include histone H4 lysine 16, p53, and alpha-tubulin.

4 ranslational retyrosination of detyrosinated alpha-tubulin.

5 IM1 and Orai1, accompanied by hypoacetylated alpha-tubulin.

6 erent post-translationally modified forms of alpha-tubulin.

7 ncer cells was accompanied by hypoacetylated alpha-tubulin.

8 ed cell motility is through deacetylation of alpha-tubulin.

9 lation of its cytoplasmic substrates such as alpha-tubulin.

10 s including histone H3 (H3), p53, HSP90, and alpha-tubulin.

11 s, shrunken spindles, and hypoacetylation of alpha-tubulin.

12 sed C-terminal glutamates from detyrosinated alpha-tubulin.

13 promote cell migration via deacetylation of alpha-tubulin.

14 XPD, XRCC1, Gli1, Gli2, SHH, IHH, GAPDH and alpha-tubulin.

15 CPP-6, which promotes Delta2 modification of alpha-tubulin.

16 ated by the lack of increased acetylation of alpha-tubulin.

17 activity, resulting in the deacetylation of alpha-tubulin.

18 acts as a K40-specific acetyltransferase for alpha-tubulin.

19 vitro, MEC-17 exclusively acetylates K40 of alpha-tubulin.

20 y increasing HDAC6-mediated deacetylation of alpha-tubulin.

21 P-170 binds to the acidic C-terminal tail of alpha-tubulin.

22 in the developing mouse brain by acetylating alpha-tubulin.

23 ced by mutations in mec-12, which encodes an alpha-tubulin.

24 microtentacles are enriched in detyrosinated alpha-tubulin.

25 t the compound covalently binds to Cys316 of alpha-tubulin.

26 ompanied by HDAC6-dependent deacetylation of alpha-tubulin.

27 of insoluble parkin and, to a lesser extent, alpha-tubulin.

28 ng known substrates such as SLP-76, LAT, and alpha-tubulin.

29 nd deacetylates cytoplasmic proteins such as alpha-tubulin.

30 ronetin perturbs the T7 loop and helix H8 of alpha-tubulin.

31 ater hyperacetylation of the HDAC6 substrate alpha-tubulin.

32 ent on posttranslational "detyrosination" of alpha-tubulin.

33 GT1-7 cells with induction in acetylation of alpha-tubulin.

34 stone H4 acetylated at K16 (Ac-H4K16) and Ac-alpha-tubulin.

35 oximity to the lysine 40 acetylation site in alpha-tubulin.

36 L1/2, an antibody classically used to detect alpha-tubulin.

37 iana tortifolia2 carries a point mutation in alpha-tubulin 4 and shows aberrant cortical microtubule

38 In addition, we used a GFP-tagged alpha-tubulin 6 (GFP-TUA6) to visualise the behaviour of

39 ds of wild-type mice, PACRG colocalizes with alpha-tubulin, a marker for the manchette, whereas this

40 nt coincident with an increase in acetylated alpha-tubulin, a marker of HDAC6 inhibition, by immunohi

41 K drives the dissociation of syndecan-1 from alpha-tubulin, a molecule that may act as an anchor for

42 amide also dramatically increased acetylated alpha-tubulin, a primary substrate of SirT2, and MAP2c,

43 is hypoacetylated while hyperacetylation of alpha-tubulin, a substrate of histone deacetylase 6 (HDA

44 , it also increased the acetylation level of alpha-tubulin, a well-established SIRT2 substrate, in bo

45 with deacetylase family member 6 (HDAC6) and alpha-tubulin acetyl transferase (alpha-TAT1).

46 h paired biopsies all had increases in tumor alpha-tubulin acetylation after treatment.

47 Myc-nick induces alpha-tubulin acetylation and altered cell morphology by

48 and its paralog alphaTAT-2 are required for alpha-tubulin acetylation and for two distinct types of

49 on-induced GCN5 degradation, thus recovering alpha-tubulin acetylation and G2/M progression.

50 ocumented the relationship between increased alpha-tubulin acetylation and the aggressive behaviors o

51 his study, we report that elevated levels of alpha-tubulin acetylation are a sufficient cause of meta

52 show that the loss of a single modification, alpha-tubulin acetylation at Lys-40, influences the bind

53 alpha-Tubulin acetylation at Lys-40, located on the lumi

54 By mimicking alpha-tubulin acetylation genetically, we show both cell

55 738 and ACY-775 induce dramatic increases in alpha-tubulin acetylation in brain and stimulate mouse e

56 t Mec-17 and its paralog are responsible for alpha-tubulin acetylation in Caenorhabditis elegans.

57 g to depletion of ATP and robustly increased alpha-tubulin acetylation in cancer cells.

58 with alpha-tubulin, and it is essential for alpha-tubulin acetylation in EMT.

59 ng novel light on the physiological roles of alpha-tubulin acetylation in mammals.

60 nous SIRT2 expression correlate with reduced alpha-tubulin acetylation in primary mouse cortical neur

61 on and death in patients with high-intensity alpha-tubulin acetylation in primary tumors.

62 t1-null animals display no overt phenotypes, alpha-tubulin acetylation is lost in sperm flagella and

63 etastatic breast cancer cells exhibited high alpha-tubulin acetylation levels that extended along mic

64 processes, and migrating cells are marked by alpha-tubulin acetylation on lysine 40, a modification t

65 cate that by influencing cellular stiffness, alpha-tubulin acetylation sets the force required for to

66 Reducing alpha-tubulin acetylation significantly inhibited migrat

67 inding of alphaTAT1 onto highly concentrated alpha-tubulin acetylation sites.

68 the aggregation-prone mutant SOD1 increased alpha-tubulin acetylation, and the acetylation-mimicking

69 ng of histone deacetylase 6 (HDAC6) increase alpha-tubulin acetylation, endoplasmic reticulum (ER)-mi

70 digit muM level by using the Sirt2 substrate alpha-tubulin-acetylLys40 peptide and inactive up to 100

71 atic activity of the Caenorhabiditis elegans alpha-tubulin acetyltransferase (alpha-TAT) MEC-17 allow

72 alpha-Tubulin acetyltransferase (alphaTAT1) is the major

73 Although it is known that the alpha-tubulin acetyltransferase (alphaTAT1) is the prima

74 There is one such protein known as Atat1 (alpha-tubulin acetyltransferase 1) per higher organism.

75 microtubules, mec-17;atat-2 mutants lacking alpha-tubulin acetyltransferase activity have short micr

76 Here we demonstrate that mice lacking the alpha-tubulin acetyltransferase Atat1 in sensory neurons

77 Here, we present the structure of the human alpha-tubulin acetyltransferase catalytic domain bound t

78 ysine acetyltransferases of known structure, alpha-tubulin acetyltransferase displays a relatively we

79 We demonstrate here that Atat1 is a major alpha-tubulin acetyltransferase in mice.

80 been limited by the undefined status of the alpha-tubulin acetyltransferase.

81 catalysis, demonstrating that the family of alpha-tubulin acetyltransferases uses a reaction mechani

82 ral and mechanistic information available on alpha-tubulin acetyltransferases.

83 cells that carry a temperature-sensitive Glu-alpha-tubulin allele.

84 explore a potential mechanism of acetylated alpha-tubulin (alpha-Ac-Tub) regulation by Ac-SDKP.

85 ascomycetes likely possessed two paralogs of alpha-tubulin (alpha1/alpha2) and beta-tubulin (beta1/be

86 Ulk4 also regulated acetylation of alpha-tubulin, an important post-translational modificat

87 Using a proteomics approach, we identified alpha tubulin and beta tubulin as proteins that interact

88 turn modify cytoplasmic proteins, including alpha-tubulin and ATG3.

89 inding sites include the H12 helices of both alpha-tubulin and beta-tubulin and are significant for C

90 rometry, we find that CLIP-170 binds to both alpha-tubulin and beta-tubulin, and that binding is not

91 correctly spliced mRNAs, including those for alpha-tubulin and Bub1, and impairs cell cycle progressi

92 ld microscopy using the double staining with alpha-tubulin and centrin antibodies strongly suggested

93 Mutation of the acetylation site on alpha-tubulin and enzymatic modulation of this posttrans

94 d the subsequent up-regulation of acetylated alpha-tubulin and FGF-21.

95 ry staining of the cells with antibodies for alpha-tubulin and fibronectin showed no difference betwe

96 sion levels of eight genes (SSU-rDNA, actin, alpha-tubulin and five beta-tubulin sequences) to their

97 altered morphology and decreased acetylated alpha-tubulin and glu-tubulin.

98 tionally crucial C-terminal tail sequence of alpha-tubulin and how this interaction catalyzes the tyr

99 inding site, and it selectively deacetylases alpha-tubulin and Hsp90.

100 to detyrosinate the C-terminal EEY region of alpha-tubulin and indicates that it is a candidate for t

101 atural product pironetin is known to bind to alpha-tubulin and is a potent inhibitor of microtubule p

102 utoantigens such as vimentin, collagen V, or alpha-tubulin and it has been postulated that autoantibo

103 of its effect on the structural integrity of alpha-tubulin and its interaction with TBCB.

104 at SDF-1 treatment induced IQGAP1 binding to alpha-tubulin and localization to CXCR4-containing endos

105 enerated flies expressing fusion proteins of alpha-tubulin and rsEGFP2 highlighting the microtubule c

106 on was demonstrated via increased acetylated alpha-tubulin and SOX9 proteins, the number of primary c

107 lar details of the pironetin binding site on alpha-tubulin and thus offer a promising basis for the r

108 gene editing to tag a cytoskeletal protein (alpha-tubulin) and demonstrate a relationship between ex

109 otubule lattice, increased detyrosination of alpha-tubulin, and altered redox signaling.

110 dundantly required for acetylation of MEC-12 alpha-tubulin, and contribute to the function of touch r

111 also clearly probe the intrinsic beta-actin, alpha-tubulin, and glyceraldehyde 3-phosphate dehydrogen

112 In addition, ARHGAP21 interacts with alpha-tubulin, and it is essential for alpha-tubulin ace

113 s including histones, transcription factors, alpha-tubulin, and nuclear importers.

114 GCN5 associated with and acetylated alpha-tubulin, and recovering GCN5 protein levels in p38

115 creases HDAC6 activity, increases acetylated alpha-tubulin, and reduces cell migration.

116 deacetylates nonhistone proteins, including alpha-tubulin, and regulates cell motility.

117 MBS and P-MBS contents were normalized to alpha-tubulin, and ROCK activity was expressed as the ra

118 -42 promoted hyperacetylation of H3, H4, and alpha-tubulin, and up-regulation of p21.

119 Immunofluorescence staining with anti-alpha-tubulin antibodies and cell cycle analysis indicat

120 Here, we show that alpha-tubulin appears to be associated with the PADI6/CP

121 Compounds that bind to alpha-tubulin are less well characterized and unexploite

122 hydrolysis trigger conformational changes in alpha-tubulin around an "anchor point," leading to globa

123 Using detection of tyrosinated alpha-tubulin as a marker for new or growing microtubule

124 against Phe-Met-Arg-Phe-NH(2) and acetylated alpha-tubulin as well as intracellular injections of Neu

125 ly impaired HDAC6-dependent deacetylation of alpha-tubulin as well as the expression of EMT markers.

126 on of stable detyrosinated microtubules (Glu-alpha-tubulin), as previously shown in Chinese hamster o

127 ided intracellularly closely associated with alpha-tubulin, as in vivo.

128 Because detyrosinated alpha-tubulin associates strongly with intermediate fila

129 tical neuronal migration upon acetylation of alpha-tubulin at lysine 40 by the histone acetyltransfer

130 tion (H3K36me3) of histones, also methylates alpha-tubulin at lysine 40, the same lysine that is mark

131 support a model in which PKC phosphorylates alpha-tubulin at Ser165, leading to microtubule elongati

132 quired to increase the levels of tyrosinated alpha-tubulin at the axon injury site and plays an impor

133 LARG colocalizes with alpha-tubulin at the spindle poles before localizing to

134 with the 3'UTR of a constitutively expressed alpha-Tubulin, Bam became stabilized in spermatocytes.

135 The tagged proteins include alpha tubulin, beta actin, desmoplakin, fibrillarin, nuc

136 Isolated Ribbons contain acetylated alpha-tubulin, beta-tubulin, conserved protein Rib45, >9

137 ut is unique in its active site and putative alpha-tubulin binding site.

138 nofluorescence microscopy) of caveolin-3 and alpha-tubulin; both Colch and CD decreased co-localizati

139 nd interacts with the phosphate group of the alpha-tubulin-bound GTP.

140 functional loop directly associates with the alpha-tubulin-bound guanosine triphosphate (GTP) molecul

141 of the ubiquitin conjugation system include alpha-tubulin (but not beta-tubulin), a dynein subunit (

142 ound to induce elevated levels of acetylated alpha-tubulin, but not histone, consistent with its HDAC

143 nd re-ligation of the C-terminal tyrosine on alpha-tubulin, but the purpose of this tyrosination-dety

144 g the injury-induced increase in tyrosinated alpha-tubulin by knocking down TTL impairs retrograde or

145 ly because p38IP promotes the acetylation of alpha-tubulin by preventing the degradation of GCN5, in

146 A new study shows that phosphorylation of alpha-tubulin by the atypical kinase PHS1 leads to rapid

147 arked is by posttranslational acetylation of alpha-tubulin by tubulin acetyltransferase (TAT).

148 d the LINKIN interactors RUVBL1, RUVBL2, and alpha-tubulin by using SILAC mass spectrometry on human

149 lin lysine 40 (K40) or detyrosination of the alpha-tubulin C-terminal tail.

150 e region containing motifs homologous to the alpha-tubulin C-terminal tail.

151 Cellular alpha-tubulin can bear various carboxy-terminal sequence

152 ), a cytosolic enzyme that, by deacetylating alpha-tubulin, can alter the stability of microtubules a

153 RARRES1 increases the level of detyrosinated alpha-tubulin consistent with a role as the cognate inhi

154 ose association with the cytoskeletal marker alpha-tubulin, consistent with growth of neuronal tracts

155 Here, we show that acetylation of MEC-12 alpha-tubulin constrains protofilament number in C. eleg

156 utes to a binding pocket that interacts with alpha-tubulin contained in the longitudinally adjacent a

157 n through an inhibitory interaction with the alpha-tubulin deacetylase HDAC6.

158 oplasmic deacetylase that uniquely catalyzes alpha-tubulin deacetylation and promotes cell motility.

159 was to investigate the effect of EGF-induced alpha-tubulin deacetylation through activating HDAC6 on

160 at involves EGF-induced HDAC6 activation and alpha-tubulin deacetylation, subsequently affecting corn

161 sure the fraction of [(13)C6]leucine-labeled alpha-tubulin-derived surrogate peptides.

162 egulates tubulin tyrosine ligase, increasing alpha-tubulin detyrosination and promoting microtentacle

163 ctopic expression of Twist or Snail promotes alpha-tubulin detyrosination and the formation of tubuli

164 This increase was supported by elevated alpha-tubulin detyrosination and vimentin protein levels

165 Finally, we show that the levels of alpha-tubulin detyrosination remain significantly elevat

166 In Chlamydomonas, GFP-tagged alpha-tubulin enters cilia as an intraflagellar transpor

167 d end binding protein 1 (EB1) and YFP-tagged alpha-tubulin expressed in the same cell revealed that t

168 is a reduced efficiency whereby quasinative alpha-tubulin folding intermediates are generated via AT

169 We show that the mCerulean-alpha-tubulin fusion protein localizes to the cytoskelet

170 IS1, DCX, ARX and RELN--mutations in a human alpha-tubulin gene, TUBA1A, have recently been identifie

171 , sequence analysis of human, mouse, and rat alpha-tubulin genes has enabled an updated nomenclature

172 esulting in an accumulation of detyrosinated alpha-tubulin (Glu-tubulin), and increases microtentacle

173 d points included p53, c-Jun, histone H2A.x, alpha-tubulin, histone H3, alpha tubulin, mitochondrial

174 embranes contained predominantly tyrosinated alpha-tubulin; however, aPKCiota was the limiting and es

175 opic expression of GCN5 efficiently reversed alpha-tubulin hypoacetylation and G2/M arrest.

176 Analysis of the forms of alpha tubulin in the olfactory bulb of mice lacking Nna1

177 cellular distribution of soluble tyrosinated alpha-tubulin in Elp1-deficient primary sympathetic and

178 Compound 17 increased acetylated alpha-tubulin in HCT116 colon tumor cells 253-fold but o

179 ment with MTs and that appearance of labeled alpha-tubulin in the dimer pool may be a consequence of

180 DIP13 partially co-localises with acetylated alpha-tubulin in the insect procyclic stage of the paras

181 acetyltransferase (TAT) acetylates Lys-40 of alpha-tubulin in the microtubule lumen.

182 essing of Glu residues from beta- as well as alpha-tubulin in vitro and in vivo.

183 ELP3, and GCN5, have been shown to acetylate alpha-tubulin in vitro, so an important question is how

184 ha-tubulins of one protofilament lie next to alpha-tubulins in the neighboring protofilaments, or the

185 e expression of house-keeping genes (e.g. Cl alpha-tubulin) in these transfectants.

186 alpha-Tubulin incorporates only during new basal body as

187 13 expression led to elevation of acetylated alpha-tubulin, indicating increased microtubule stabilit

188 They also increased the acetylation level of alpha-tubulin, indicating that SIRT2 is likely to be the

189 cells, incorporation of 3-nitrotyrosine into alpha-tubulin induces a progressive, reversible reorgani

190 nation, a post-translational modification of alpha-tubulin, influences X-ROS signalling, contraction

191 t ARHGAP21 affects migration and EMT through alpha-tubulin interaction and acetylation.

192 dinal interfaces, as well as movement of the alpha-tubulin intermediate domain and H7 helix.

193 ylation of the epsilon-amino group of K40 on alpha-tubulin is a conserved PTM on the luminal side of

194 An essential component of microtubules, alpha-tubulin is also a multigene family in many species

195 Acetylation of alpha-tubulin is an essential constraint on protofilamen

196 Reversible acetylation of alpha-tubulin is an evolutionarily conserved modificatio

197 Reversible detyrosination of alpha-tubulin is crucial to microtubule dynamics and fun

198 These results suggest that newly synthesized alpha-tubulin is first incorporated into MTs or complexe

199 nd GB-II-5 on apicomplexan and kinetoplastid alpha-tubulin is proposed.

200 alpha-Tubulin is specifically acetylated on lysine 40, a

201 Acetylation of Lys-40 on alpha-tubulin is unique in that it is located on the lum

202 The tuba1a gene encodes a neural-specific alpha-tubulin isoform whose expression is restricted to

203 he proteins affected include TUBA1A, a major alpha-tubulin isoform, and microtubule-associated compon

204 effects were accompanied by modifications of alpha-tubulin isoforms in the hippocampus, amygdala, and

205 jection of MAP4343 changes the expression of alpha-tubulin isoforms indicative of increased microtubu

206 n microtubule function, including changes in alpha-tubulin isoforms.

207 We conclude that this cell-specific alpha-tubulin isotype dictates the hallmarks of CEM cili

208 We show that Tuba8, another alpha-tubulin isotype previously associated with cortica

209 nesin-2 OSM-3/KIF17, whereas a cell-specific alpha-tubulin isotype regulates ciliary ultrastructure,

210 Using C. elegans, we show that alpha-tubulin isotype TBA-6 sculpts 18 A- and B-tubule s

211 Mutation of the alpha-tubulin isotype TUBA1A is associated with cortical

212 and cilia, and yet the physiological role of alpha-tubulin K40 acetylation is elusive.

213 Because alpha-tubulin K40 acetylation is largely eliminated by d

214 that it is the central determining factor of alpha-tubulin K40 acetylation levels in vivo.

215 of alphaTAT1 is necessary and sufficient for alpha-tubulin K40 acetylation.

216 at we name alphaTAT1, with a highly specific alpha-tubulin K40 acetyltransferase activity and a catal

217 Although genetic ablation of the alpha-tubulin K40 acetyltransferase alphaTat1 in mice di

218 alphaTAT1 is the major and possibly the sole alpha-tubulin K40 acetyltransferase in mammals and nemat

219 acetylases because cells fail to deacetylate alpha-tubulin-K40 and histone H3-K9.

220 sibility of this approach by applying it for alpha-tubulin labeling.

221 acellular peptides but altered the levels of alpha-tubulin lacking two C-terminal amino acids (delta2

222 ntify a tight correlation between acetylated alpha-tubulin levels and aggressive metastatic behavior

223 on in cultured neurons reduced detyrosinated alpha-tubulin levels and caused severe differentiation d

224 length Myc diminish, Myc-nick and acetylated alpha-tubulin levels are increased.

225 tofilaments, or the "A" configuration, where alpha-tubulins lie beside beta-tubulins.

226 hich TTL increases the levels of tyrosinated alpha-tubulin locally at the injury site to facilitate t

227 odification, the acetylation of lysine 40 of alpha-tubulin, located in the lumen of microtubules, is

228 fy a critical genetic interval harboring two alpha tubulin loci, and we identify a chemically induced

229 Acetylation of alpha-tubulin Lys40 by tubulin acetyltransferase (TAT) i

230 either enriched in or lacking acetylation of alpha-tubulin lysine 40 (K40) or detyrosination of the a

231 n acetyltransferase (alphaTAT1) is the major alpha-tubulin lysine-40 (K40) acetyltransferase in mamma

232 tion of the epsilon-amino group of Lys-40 of alpha-tubulin marks stable microtubules, although the ca

233 n, histone H2A.x, alpha-tubulin, histone H3, alpha tubulin, mitochondrial membrane potential, mitocho

234 Specific posttranslational alpha-tubulin modifications are critical for adherent ce

235 b deacetylase that specifically deacetylates alpha-tubulin, modulates microtubule dynamics, and promo

236 In this study, we isolated two alpha-tubulin (Msalpha1, Msalpha2) and two beta-tubulin

237 the peribiliary network stain positively for alpha-tubulin, mucins, and chromogranin A, as well as fo

238 tylation, and the acetylation-mimicking K40Q alpha-tubulin mutant promoted mutant SOD1 aggregation.

239 ctively reversed by an acetylation-mimicking alpha-tubulin mutant.

240 Tetrahymena MEC-17 gene phenocopies the K40R alpha-tubulin mutation and makes microtubules more labil

241 unction in the conserved MAPKKK dlk-1 and an alpha-tubulin mutation, specifically blocks synapse remo

242 We identified four beta-tubulin and two alpha-tubulin mutations in patients with a spectrum of c

243 tation of the TUBA8 gene, encoding a variant alpha-tubulin of unknown function that is not susceptibl

244 nct arrangements: "B-lattices," in which the alpha-tubulins of one protofilament lie next to alpha-tu

245 was accompanied by increased acetylation of alpha-tubulin on Lys40.

246 Acetylation of alpha-tubulin on lysine 40 marks long-lived microtubules

247 s of either the MEC-7 beta-tubulin or MEC-12 alpha-tubulin or by growth in 1 mM colchicine causes tou

248 tive spindle function resulting from altered alpha-tubulin or class III beta-tubulin overexpression.

249 ng enhanced green fluorescent protein-tagged alpha-tubulin or EB1 protein.

250 s of either the MEC-7 beta-tubulin or MEC-12 alpha-tubulin or growth in 1 mM colchicine) cause a gene

251 in the complex B component IFT74/72, but not alpha-tubulin or p28, a component of an inner dynein arm

252 d delta2-tubulin from purified porcine brain alpha-tubulin or polymerized HEK293T microtubules.

253 ssing either green fluorescent protein (GFP)-alpha-tubulin or the microtubule plus-end binding protei

254 ), a major posttranslational modification of alpha-tubulin, or whether proteolytic cleavage of the C-

255 , tissue-selective TTLL-mediated pathway for alpha-tubulin polyglutamylation that is required for spe

256 in conjunction with substantial reduction in alpha-tubulin polyglutamylation, which closely correlate

257 The alpha-tubulin protein distribution, centrin translation,

258 with GCN5 increased, and the acetylation of alpha-tubulin reached a peak.

259 relocalization to promote its activation and alpha-tubulin recruitment during migration.

260 Acetylation of alpha-tubulin residue Lysine-40 (K40) has been correlate

261 vity, as well as by the identification of an alpha-tubulin residue specifically required for the Kip3

262 ively abolished EGF-induced deacetylation of alpha-tubulin, resulting in the inhibition of cell migra

263 -hydroxybenzamide (3f) on the acetylation of alpha-tubulin revealed an increased level of acetylation

264 t measured SIRT2 deacetylation of acetylated alpha-tubulin revealed that propofol inhibits enzymatic

265 molecular dynamics simulations-suggest that alpha-tubulin's amphipathic helix H10 is responsible for

266 on interdimer contacts, specifically on the alpha-tubulin side, and to a lesser extent on interproto

267 dies for each of the four proteins analyzed (alpha-tubulin, Spag6, centrin, and Sas6a).

268 ctural elements that play important roles in alpha-tubulin-specific acetylation.

269 mutations in the M loop (Ala273Val) or in an alpha-tubulin-specific insert that stabilizes the M loop

270 ial elastic modulus of 1.10 +/- 0.14 GPa for alpha-tubulin structures and 1.39 +/- 0.68 GPa for beta-

271 ificity is achieved by interactions with the alpha-tubulin substrate that extend outside of the modif

272 les became rapidly stabilized and that their alpha-tubulin subunit posttranslationally detyrosinated

273 inhibiting severing, and that detyrosinated alpha-tubulin tails are the least effective.

274 sequences of alpha-, beta-, or detyrosinated alpha-tubulin tails that have been covalently linked to

275 a provide a basis for the rational design of alpha-tubulin targeting chemotherapeutics.

276 es, and tissues were analyzed for acetylated alpha-tubulin, tau-1, and p53 expression when possible.

277 Baseline or cycle 2 acetylated alpha-tubulin, tau-1, or p53 expression did not correlat

278 re, we find that three tubulin isotypes--the alpha-tubulins TBA-6 and TBA-9 and the beta-tubulin TBB-

279 We also discovered an alpha-tubulin (TBA-7) that appears to destabilize MTs.

280 rafish expressing a photoconvertible form of alpha-tubulin (tdEOS-tubulin) specifically in cone photo

281 showed strong phosphorylation of endogenous alpha-tubulin that could be blocked when S165N-alpha6-tu

282 een stathmin's N terminus and the surface of alpha-tubulin that is exposed only at the minus end.

283 he distribution of tyrosinated/detyrosinated alpha-tubulin that is recruited by Rab2 in a quantitativ

284 an enzyme that acetylates the K40 residue of alpha-tubulin, the only PTM known to occur on the lumina

285 This result indicates that the alpha-tubulin tyrosine facilitates initial motor-tubulin

286 lts reveal a strong effect of the C-terminal alpha-tubulin tyrosine on dynein-dynactin motility and s

287 ese effects resulted in decreased parkin and alpha-tubulin ubiquitination, accumulation of insoluble

288 provide preclinical rationale for acetylated alpha-tubulin use as a pharmacodynamic biomarker in futu

289 approximately half of KIF17 and one third of alpha-tubulin utilizes diffusion besides IFT.

290 tin forms a covalent bond to cysteine-316 in alpha-tubulin via a Michael addition reaction.

291 The level or distribution of acetylated alpha-tubulin was not altered in HDAC3-deficient cells.

292 ause GAPDH binds to the carboxyl terminus of alpha-tubulin, we characterized the distribution of tyro

293 Levels of acetylated alpha-tubulin were decreased in the ischemic hemisphere

294 ional effects on the C-terminal H12 helix of alpha-tubulin, which is a likely molecular mechanism for

295 the processing of cytosolic proteins such as alpha-tubulin, which is known to be modified by the remo

296 alphaAsn258, and alphaLys352 amino groups in alpha-tubulin, which supported the formation of a covale

297 ulates microtubule function by deacetylating alpha-tubulin, which suppresses microtubule dynamics and

298 ring the G2/M transition, the association of alpha-tubulin with GCN5 increased, and the acetylation o

299 f calibrations, we site-specifically labeled alpha-tubulin with silicon rhodamine (SiR) in live mamma

300 nd beta-tubulin and also generated a form of alpha-tubulin with two C-terminal Glu residues removed (