ライフサイエンスコーパス: alphoid

1 The alphoid array of one chromosome is quite small and below

2 nsertion, designated LY1, in the centromeric alphoid array of the human Y chromosome.

3 s of DNA replication and that replication of alphoid arrays organized into centrochromatin occurred e

4 of inter-origin distances within centromeric alphoid arrays was comparable to the distribution of int

5 me constructs minimally containing competent alphoid arrays, a selectable marker and terminal human t

6 These alphoid BACs were found to be highly unstable in Sch. po

7 estigated by transfecting circular or linear alphoid constructs with or without human telomere arrays

8 lar HAC vectors, two with chromosome 17 or Y alphoid DNA (17alpha, Yalpha) and two with 17alpha or Ya

9 ce for histone turnover/exchange activity on alphoid DNA and prevents Suv39h1-mediated heterochromati

10 of interest, one can either co-transfect the alphoid DNA and the gene of interest, or one can clone b

11 Both CENP-B and its recognition box in alphoid DNA are conserved in mammals, suggesting an impo

12 A construct with an alphoid DNA array from chromosome 22 with no detectable

13 The alphoid DNA array was also isolated from the 12 Mb human

14 Alphoid DNA has been shown to be sufficient for human ce

15 To investigate the stability of alphoid DNA in fission yeast, bacterial artificial chrom

16 antially stabilizes the CENP-A nucleosome on alphoid DNA in human cells.

17 on of KGF-like sequences suggests a role for alphoid DNA in their amplification and dispersion.

18 Tethering KAT7 to an ectopic alphoid DNA integration site removed heterochromatic H3K

19 by transfection of large fragments of cloned alphoid DNA into human HT1080 cells in tissue culture.

20 somes with less than approximately 100 kb of alphoid DNA is very inefficient, suggesting that a funct

21 A TAR vector with alphoid DNA monomers as targeting sequences was used to

22 e we describe linking approximately 70 kb of alphoid DNA onto a 156-kb BAC carrying the human HPRT ge

23 ltaYq74 that contained the minimum amount of alphoid DNA required for proper chromosome segregation.

24 le containing 100 kb of highly homo- geneous alphoid DNA retrofitted with human telomere repeats.

25 ate centromere with two blocks of functional alphoid DNA separated by 2.5 Mb can exist as a stable st

26 an be used to add the approximately 70 kb of alphoid DNA to any BAC carrying a gene of interest to ge

27 The >300-kb BAC carrying alphoid DNA was successfully delivered to 293A and HT108

28 Retrofitting of PACs and BACs carrying alphoid DNA was very efficient with almost no rearrangem

29 omosomes (BACs) containing 130 and 173 kb of alphoid DNA were retrofitted with the Sch. pombe ars1 el

30 episomal maintenance in mammalian cells, or alphoid DNA, which allows human artificial chromosome fo

31 st no rearrangement of the highly repetitive alphoid DNA.

32 equires a minimum of approximately 100 kb of alphoid DNA.

33 nce and to stably maintain long stretches of alphoid DNA.

34 mbles on a part of the long alpha-satellite (alphoid) DNA array, where it is flanked by pericentric h

35 some (HAC) vectors based on alpha-satellite (alphoid) DNA from chromosome 17 but not the Y chromosome

36 circular HACs containing multiple copies of alphoid fragments (60-250 kb) interspersed with either v

37 equences as stuffer DNA: a human fragment of alphoid repeat DNA, matrix-attachment regions (MARs), an

38 uently contain HIV integrated in or close to alphoid repeat elements in heterochromatin.

39 cts and 23 HIV-2-infected subjects, using an alphoid repeat PCR assay.

40 specific PCR-based assay also indicated that alphoid repeats were disfavored for integration in vivo

41 However, we did find that centromeric alphoid repeats were selectively absent at integration s

42 ci and a nucleotide substitution in the DYZ3 alphoid satellite locus).

43 ement, and in situ hybridization for primate alphoid satellite sequences ubiquitous in all centromere

44 e here a new method to rapidly amplify human alphoid tandem repeats of a few hundred base pairs into

45 y, CENP-28/Eaf6-induced transcription of the alphoid(tetO) array associated with H4K12 acetylation do

46 s are simultaneously targeted to a synthetic alphoid(tetO) HAC centromere.

47 The alphoid(tetO)-HAC elimination is highly efficient when a

48 To adopt the alphoid(tetO)-HAC for routine gene function studies, we

49 The recently developed alphoid(tetO)-HAC has an advantage over other HAC vector

50 The recently developed alphoid(tetO)-HAC has an advantage over other HAC vector

51 subsequent loading into the loxP site of the alphoid(tetO)-HAC in hamster CHO cells from where the HA

52 e describe an approach to re-engineering the alphoid(tetO)-HAC that allows verification of phenotypic

53 egulating fluctuating heterochromatin on the alphoid(tetO)-HAC that induces fast silencing of the gen

54 The newly modified alphoid(tetO)-HAC-based system has multiple applications

55 phenotypes induced by genes loaded into the alphoid(tetO)-HAC.

56 (and control untransfected and 'irrelevant' alphoid YAC transfectant A9 clones) were assayed for in