1 The "
amadoriase"
activity was also inhibited by Hg2+, Ag2+, C
2 Amadoriases are a novel class of FAD enzymes which catal
3 Amadoriases are of potential importance as tools for unc
4 rch in various data bases revealed that this
amadoriase enzyme is a novel protein.
5 Four "
amadoriase"
enzyme fractions, which oxidatively degrade
6 t to previous described enzymes, these novel
amadoriase enzymes can deglycate both glycated amines an
7 Molecular masses of
amadoriase enzymes Ia, Ib, and Ic were 51 kDa, and 49 kD
8 these data show the presence of two distinct
amadoriase enzymes in the Aspergillus sp. soil strain se
9 The redox potentials of
amadoriase have been measured at pH 7.0, 25 degrees, giv
10 riase II revealed that two glutamic acids in
amadoriase I align to Arg(112) and Arg(114) in amadorias
11 Sequence alignment between
amadoriase I and amadoriase II revealed that two glutami
12 contrast, the corresponding K(m) values for
amadoriase I are 9.75 and 0.023 mM, respectively.
13 The mechanism of action of
amadoriase I from Aspergillus sp. has been investigated
14 Sequence alignment studies show that
amadoriase I has 22% homology with monomeric sarcosine o
15 To identify the primary structure of
amadoriase I, we prepared a cDNA library from Aspergillu
16 Amadoriases I and II are deglycation isoenzymes from Asp
17 Site-directed mutation of
amadoriase II (R112E, R114E) resulted in reversal of the
18 e and isolated a clone using polyclonal anti-
amadoriase II antibody.
19 The identity of the
amadoriase II cDNA was further confirmed by expression i
20 Amadoriase II has a preference for anionic substrate wit
21 Sequence alignment between amadoriase I and
amadoriase II revealed that two glutamic acids in amador
22 14) are responsible for the high affinity of
amadoriase II toward anionic substrates and determine th
23 Northern blotting analysis revealed that
amadoriase II was induced by fructosyl propylamine in a
24 Chemical modification of
amadoriase II with p-hydroxyphenylglyoxal, a specific ar
25 illus fumigatus and the molecular cloning of
amadoriase II.
26 s Ia, Ib, and Ic were 51 kDa, and 49 kDa for
amadoriase II.
27 adoriase I align to Arg(112) and Arg(114) in
amadoriase II.
28 Amadoriase is a flavoenzyme that catalyzes the oxidative
29 Amadoriase is an enzyme catalyzing the oxidative deglyca
30 the purification and characterization of two
amadoriase isoenzymes from Aspergillus fumigatus and the
31 the purification and characterization of two
amadoriase isozymes from Aspergillus sp. that degrade bo