ライフサイエンスコーパス: amanitin

1 inhibitors actinomycin D, DRB, H7 and alpha-amanitin.

2 inhibition of ongoing transcription by alpha-amanitin.

3 ranscription was globally inhibited by alpha-amanitin.

4 stant to the transcriptional inhibitor alpha-amanitin.

5 the effect with low concentrations of alpha-amanitin.

6 uming transcription in the presence of alpha-amanitin.

7 of transcription as it is resistant to alpha-amanitin.

8 tivity of stalled transcription complexes to amanitin.

9 polymerase II, the subunit that binds alpha-amanitin.

10 age in arrested complexes is not affected by amanitin.

11 on of endogenous Pol II using a toxin, alpha-amanitin.

12 as does inhibition of transcription by alpha-amanitin.

13 l chromatin insulators are affected by alpha-amanitin.

14 inished by treatment of the cells with alpha-amanitin.

15 ex, which is specifically inhibited by alpha-amanitin.

16 n active transcription and arrested by alpha-amanitin.

17 lectively resistant to inhibition with alpha-amanitin.

18 racts with the transcription inhibitor alpha-amanitin.

19 scription in Giardia is resistant to 1 mg/ml amanitin.

20 iptional inhibitors, actinomycin D and alpha-amanitin.

21 high concentrations (100 microg/ml) of alpha-amanitin.

22 D-ribofuranosylbenzimidazole (DRB) and alpha-amanitin.

23 merase inhibitors on HDV genome replication: amanitin, 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazo

24 oding genes is relatively resistant to alpha-amanitin (50% inhibition = 250 microg alpha-amanitin/ml)

25 hat is resistant to the mushroom toxin alpha-amanitin, a characteristic of transcription by RNA pol I

26 riole overduplication are abrogated by alpha-amanitin, a potent and specific RNA pol II inhibitor.

27 pol II dephosphorylation confirmed by alpha-amanitin, a specific RNA pol II inihibitor, showing pote

28 presence of the translocation blocker alpha-amanitin, a steady state condition is established in whi

29 alpha-Amanitin, a transcription inhibitor, did not suppress cy

30 Amanitin also greatly slows pyrophosphorolysis by elonga

31 hibition of zygotic transcription with alpha-amanitin also induced apoptosis.

32 of Pol II is significantly induced by alpha-amanitin, an amatoxin that blocks Pol II elongation and

33 rigera, AMA1 and PHA1, directly encode alpha-amanitin, an amatoxin, and the related bicyclic heptapep

34 ery of a polar cell-impermeable toxin, alpha-amanitin, an inhibitor of RNA polymerase II.

35 e II (RNAPII) translocation inhibitors alpha amanitin and 5,6-dichloro-1-beta-D-ribobenzimidazole (DR

36 hepatocytes were also observed in the alpha-amanitin and acetaminophen-induced liver injury mouse mo

37 e show that transcriptional inhibitors alpha-amanitin and actinomycin D specifically disrupt the symm

38 ion factor eIF-4C that is inhibited by alpha-amanitin and correlated with a transient increase in the

39 of G. lamblia RNAP II transcription to alpha-amanitin and found that unlike most other eukaryotes, RN

40 pared with 8-cell embryos treated with alpha-amanitin and MII.

41 In Amanita bisporigera, alpha-amanitin and phallacidin are synthesized as 35- and 34-a

42 alpha-Amanitin and phallacidin are synthesized as proproteins

43 riptional inhibitors actinomycin D and alpha-amanitin and requires the kinase activity of ATM but not

44 manitin but sensitive to 100 microg/ml alpha-amanitin and tagetitoxin, suggesting involvement of RNA

45 e II that is relatively insensitive to alpha-amanitin and that differs from typical eukaryotic RNA po

46 uire an RNA primer, are insensitive to alpha-amanitin, and differ in their ability to displace the no

47 endent RNA polymerases (actinomycin D, alpha-amanitin, and rifampin).

48 localized with RNA polymerase II in an alpha-amanitin- and actinomycin D-sensitive manner.

49 side triphosphate (NTP) substrates and alpha-amanitin are added to a human RNA polymerase II elongati

50 e of NTPs resume RNA synthesis when NTPs and amanitin are added.

51 Using alpha-amanitin as a dynamic probe of the RNA polymerase II mec

52 d to correlate with the sensitivity to alpha-amanitin, as S. pombe was intermediate between human and

53 nomic HDV RNA was totally inhibited by alpha-amanitin at concentrations as low as 2.5 microg/ml.

54 Of most interest, amanitin at low doses blocked accumulation of HDV RNA-di

55 However, we found that alpha-amanitin-based antibody-drug conjugates are highly effec

56 The amanitin motif (amanitin binding site) of Rpb1 from G. lamblia has amino

57 ed by co-incubation with picrotoxin or alpha-amanitin but is insensitive to nifedipine, indicating th

58 tant to the RNA polymease II inhibitor alpha-amanitin but is sensitive to short interfering RNA speci

59 of TARE-6 was resistant to 1 microg/ml alpha-amanitin but sensitive to 100 microg/ml alpha-amanitin a

60 Chain elongation with amanitin can continue for hours at approximately 1% of t

61 Here we show that low doses of alpha-amanitin-conjugated anti-epithelial cell adhesion molecu

62 ized by an RNA polymerase resistant to alpha-amanitin, consistent with previously published reports r

63 pHLIP-mediated cytoplasmic delivery of amanitin could create great opportunities to use the tox

64 The major mechanism of amanitin delivery is direct translocation (flip) across

65 gamma interferon or its inhibition by alpha-amanitin did not alter nucleosome occupancy, positioning

66 of pol I by cycloheximide or pol II by alpha-amanitin did not significantly affect the PNC.

67 ase II in a complex with the inhibitor alpha-amanitin has been determined by x-ray crystallography.

68 Previous clinical applications of alpha-amanitin have been limited owing to its liver toxicity.

69 Transcription inhibition by alpha-amanitin in vitro enhanced pol II ubiquitylation at ubiq

70 icrograms/ml but not 1 microgram/ml of alpha-amanitin indicates transcription of the mouse YRNA genes

71 lly renders Pol II highly resistant to alpha-amanitin, indicating a functional interaction between Hi

72 tion of the GP63 genes is sensitive to alpha-amanitin, indicating that they are transcribed by a diff

73 ription of the ESAG-Is is sensitive to alpha-amanitin, indicating that they are transcribed by a diff

74 ffected by either kinase inhibitors or alpha-amanitin-induced depletion of pol II.

75 ir sensitivity to DRB, indicating that alpha-amanitin induces apoptosis solely by inhibiting RNAP II

76 Additionally, both actinomycin D and alpha-amanitin inhibited the increase in uPA mRNA by PAF.

77 We propose that alpha-amanitin-inhibited Pol II elongation, which is slow and

78 s indicated that this apparent resistance to amanitin inhibition of genomic and antigenomic RNA relat

79 We show by alpha-amanitin inhibition that RNA polymerase II (RNAPII) tran

80 In contrast, 50 microg/ml amanitin inhibits 85% of RNAP III transcription activity

81 in the following bond addition cycle, alpha-amanitin inhibits elongation at each translocation step.

82 alpha-Amanitin insensitivity confirmed that overexpression of

83 trate selectivity, results from direct alpha-amanitin interference with the TL.

84 Although alpha-amanitin interferes with the transcript cleavage stimula

85 We show that pHLIP can deliver alpha-amanitin into cells in a pH-dependent fashion and induce

86 The cyclic octapeptide amanitin is a relatively selective inhibitor of eukaryot

87 The toxin alpha-amanitin is frequently employed to completely block RNA

88 itivity to different concentrations of alpha-amanitin is that expected for human RNA polymerase III;

89 -beta-D-ribofuranosyl-benzimadazole or alpha-amanitin leads to accumulation of cellular p53 protein.

90 as inhibited by a low concentration of alpha-amanitin (<3 microgram/ml) and could be partially restor

91 -amanitin (50% inhibition = 250 microg alpha-amanitin/ml).

92 The amanitin motif (amanitin binding site) of Rpb1 from G. l

93 presence of the translocation blocker alpha-amanitin, NTPs (but not deoxynucleotide triphosphate [dN

94 the effect of the fungus-derived toxin alpha-amanitin on the transcription of protein-coding genes of

95 Treatment of cells with alpha-amanitin or actinomycin D revealed that extension of B2

96 s blocked by cycloheximide, but not by alpha-amanitin or actinomycin D.

97 ating Pol II-DNA complexes arrested by alpha-amanitin or cisplatin lesions, triggering ubiquitination

98 Suppression of POLR2A with alpha-amanitin or small interfering RNAs selectively inhibits

99 Inhibition of transcription using alpha-amanitin, or the dissolution of R loops by transient exp

100 mplexes were slowed by the presence of alpha-amanitin, origin activity depended on the orientation of

101 model and models of acetaminophen and alpha-amanitin poisoning were used.

102 hat is supported by rerefinement of an alpha-amanitin-Pol II crystal structure.

103 Inhibition of Pol II activity using alpha-amanitin reduced expression of a number of Pol III genes

104 udy we carry out a genetic analysis of alpha-amanitin resistance in a population sample of Drosophila

105 pport previous proposals of the mechanism of amanitin resistance in other organisms and provide a mol

106 nserved sites that have been associated with amanitin resistance in other organisms.

107 These observations of amanitin resistance of Giardia RNA polymerase II support

108 Engineered expression of an alpha-amanitin resistance RNAP II gene rendered cells resistan

109 multidrug resistance gene (Mdr65A) in alpha-amanitin resistance.

110 The antigenomic RNA labeling was alpha-amanitin resistant and localized to the nucleolus.

111 s contribute to the difference between alpha-amanitin-resistant and alpha-amanitin-sensitive third ch

112 and could be partially restored by an alpha-amanitin-resistant mutant pol II; however, surprisingly,

113 Using an alpha-amanitin-resistant polymerase, we provide evidence that

114 Utilizing alpha-amanitin-resistant RNA polymerase II mutants with or wit

115 ing plasmid constructs that express an alpha-amanitin-resistant RNAP II subunit with a truncated or f

116 n immunodeficiency virus 1, we used an alpha-amanitin-resistant system developed previously.

117 script cleavage activity which is completely amanitin-resistant; furthermore, pyrophosphorolytic tran

118 ts, and inhibition of transcription by alpha-amanitin resulted in the initiation of replication withi

119 ed with the transcriptional inhibitor, alpha-amanitin, revealed that the symmetry of cell division is

120 The genomic RNA labeling was alpha-amanitin sensitive and more diffusely localized in the n

121 3 and 4.4 loci) whose transcription is alpha-amanitin sensitive.

122 firmed that HDV RNA synthesis had both alpha-amanitin-sensitive and -resistant components.

123 Based on alpha-amanitin-sensitive BrUTP incorporation, transcription in

124 eChip set, we characterized the set of alpha-amanitin-sensitive genes expressed during the 1- and 2-c

125 e between alpha-amanitin-resistant and alpha-amanitin-sensitive third chromosome lines, the underlyin

126 0 intergenic region promoter can drive alpha-amanitin-sensitive transcription at an internal position

127 was functionally sufficient to support alpha-amanitin-sensitive transcription.

128 esent on the MOE430 GeneChip set to be alpha-amanitin-sensitive.

129 nes expressed in the 2-cell embryo are alpha-amanitin-sensitive.

130 A comparison of the alpha-amanitin sensitivity of transcription in naturally occur

131 carried out by an RNA polymerase with alpha-amanitin sensitivity reminiscent of SL RNA synthesis and

132 nscription began, but experiments with alpha-amanitin show that cyclin E degradation is not dependent

133 e 50-amino-acid region thought to bind alpha-amanitin shows that this region of the trichomonad RNA p

134 could be inhibited by low concentrations of amanitin specific for Pol II transcription.

135 t of cells with actinomycin D, DRB, or alpha-amanitin, specific inhibitors of Pol II, disperses ELL a

136 that inhibition of RNAPII activity by alpha-amanitin specifically blocks processing of rRNA.

137 cription in CS-B cells is sensitive to alpha-amanitin, suggesting that it is RNA polymerase II-depend

138 llowing activation but is sensitive to alpha-amanitin, suggesting that polymerase movement is needed

139 ucleotides into RNA in the presence of alpha-amanitin, suggesting that the polymerase I enzyme is par

140 V RNAs have different sensitivities to alpha-amanitin, suggesting that these two strands are synthesi

141 polymerase inhibitors tagetitoxin and alpha-amanitin that are consistent with RNA polymerase II tran

142 tional interaction between His1085 and alpha-amanitin that is supported by rerefinement of an alpha-a

143 the two-cell stage in the presence of alpha-amanitin, this change in transcript abundance is not obs

144 b) antigenomic RNA was not affected by alpha-amanitin to a concentration higher than 25 microgram/ml.

145 nsulin receptors in cells treated with alpha-amanitin to block host cell mRNA synthesis.

146 ion capability could be tuned by conjugating amanitin to the C-terminus of pHLIP via linkers of diffe

147 ution described nonspecific binding of alpha-amanitin to yeast RNA polymerase II.

148 bit RNA polymerases I, II, and III) or alpha-amanitin (to inhibit RNA polymerases II and III) as well

149 lved in the biosynthesis of the highly toxic amanitin toxin family of macrocycles.

150 s outside the plant kingdom (e.g., the alpha-amanitin toxin gene family in the mushroom, Amanita bisp

151 chemical mutation' tools coupling with alpha-amanitin transcription inhibition assay to systematicall

152 e duplication proceeded undisturbed in alpha-amanitin-treated cells.

153 alpha-Amanitin treatment had no effect on the ratio of phospho

154 gle- and triple-knockdown experiments, alpha-amanitin treatment, transcriptome profiling and chromati

155 e SII-mediated transcript cleavage following amanitin treatment.

156 Apoptosis induced by cycloheximide or alpha-amanitin was blocked by injection of RNA encoding Xenopu

157 Following treatment with alpha amanitin we observed a profound reduction in the occupan

158 by the POP of C. albipes, but a precursor of amanitin (which is not made by C. albipes) was cleaved i

159 The addition of alpha-amanitin, which can inhibit transcription, reduced unres

160 pHLIP-SPDP-amanitin, which exhibits 4-5 times higher antiproliferat

161 D-ribofuranosylbenzimidazole (DRB) and alpha-amanitin, which inhibit RNAP II function by two distinct

162 We find that the mushroom toxin alpha-amanitin, which inhibits TL mobility, suppresses the eff

163 PADT is inhibited by alpha-amanitin, which presumably blocks the required conformat

164 resistant to induction of apoptosis by alpha-amanitin without affecting their sensitivity to DRB, ind