ライフサイエンスコーパス: amber codon

1 cysteine codon has been replaced by the UAG amber codon.

2 ally encode this initiator in response to an amber codon.

3 the MaThg1 gene and transcript confirmed the amber codon.

4 red compared to results using the three-base amber codon.

5 ural amino acid incorporation in response to amber codons.

6 ngle amber codon and from <1% to >20% on two amber codons.

7 tRNAs function efficiently in suppression of amber codons.

8 Thg1 (MaThg1) gene contains an in-frame TAG (amber) codon.

9 art and a stop that can even be a suppressed amber codon 22 nucleotides further downstream from the r

10 yl- l-phenylalanine (Bpa) in response to the amber codon allowed the biosynthesis of Bpa-substituted

11 ded this unique amino acid in response to an amber codon allowing a single 1 to be placed at any loca

12 n from approximately 20% to >60% on a single amber codon and from <1% to >20% on two amber codons.

13 red anticodons which bind to a UAG nonsense (amber) codon and to the Arg (AGG), Asn (AAC,AAT), Gln (C

14 cognate orthogonal tRNA that recognizes the amber codon, are encoded on the plasmid pSUPAR6-L3-3SY,

15 ne is a likely first step in translating UAG amber codons as pyrrolysine in certain methanogens.

16 media and the protein of interest with a TAG amber codon at the desired incorporation site.

17 1 gene product did not cease at the internal amber codon, but at the following ochre codon.

18 stal structure of MtmB demonstrated that the amber codon codes for pyrrolysine, the 22nd genetically

19 ns, each inserting a different amino acid at amber codons, created 12 different substitutions at the

20 sor tRNA gene resulted in suppression of the amber codon in a reporter chloramphenicol acetyltransfer

21 denosine to an inosine (I) within the HDAg-S amber codon in antigenomic RNA.

22 of unnatural amino acids in response to the amber codon in Escherichia coli.

23 Pyrrolysine is represented by an amber codon in genes encoding proteins such as the methy

24 Pyrrolysine is an amino acid encoded by the amber codon in genes required for methylamine utilizatio

25 oration of this amino acid in response to an amber codon in mammalian cells.

26 e activity in vitro is not solely due to the amber codon in ureD.

27 ereby, tetracycline-regulated suppression of amber codons in mammalian HeLa and COS-1 cells.

28 system that site-specifically--using the UAG amber codon--inserts Sec depending on the elongation fac

29 Expression of pylTSBCD also suppressed an amber codon introduced into the E. coli uidA gene.

30 The amber codon is recognized in the 30S subunit-decoding ce

31 The amber codon is thus read through during translation at a

32 In nearly all cases, the amber codon is used as a sense codon, and an orthogonal

33 functionally important Lys was altered to an amber codon, or to Arg, Asn, Gln, Glu, Thr and Trp codon

34 -X) improves tRNA(CUA)-dependent decoding of amber codons placed in orthogonal mRNA.

35 The amber codon position corresponded to a lysyl residue usi

36 ecodes a series of quadruplet codons and the amber codon, providing several blank codons on an orthog

37 the radical trap 3-amino tyrosine (NH2Y) by amber codon suppression at positions Y731 or Y730 and in

38 Amber codon suppression for the insertion of non-natural

39 Here we utilize amber codon suppression in a membrane-bound transporter

40 ite-specific incorporation into proteins via amber codon suppression in Escherichia coli and mammalia

41 vo incorporation of unnatural amino acids by amber codon suppression is limited by release factor-1-m

42 S can be redesigned to achieve high-fidelity amber codon suppression through delivery of p-bromopheny

43 We used amber codon suppression to introduce the photoreactive u

44 site-specific introduction into proteins via amber codon suppression using the wild-type pyrrolysyl-t

45 ters and (ii) selection of tRNA for enhanced amber codon suppression.

46 tem cells and mouse embryonic fibroblasts to amber codon suppression.

47 teins in Escherichia coli in response to the amber codon, TAG.

48 s possesses one naturally occurring in-frame amber codon that does not appear to act as a translation

49 nzymes are encoded by genes with an in-frame amber codon that is translated as pyrrolysine.

50 efficiently incorporated at a predefined UAG amber codon, thereby competing with RF1 rather than RF2.

51 fluoromethylphenylalanine in response to the amber codon UAG.

52 g the efficiency of suppression at a gene II amber codon upstream from the gene X start, the already

53 f the suppressor tRNA and suppression of the amber codon were reduced significantly in the presence o

54 into COS1 cells and acts as a suppressor of amber codons, whereas the same suppressor tRNA imported

55 ne dihydrofolate reductase in response to an amber codon with at least 98% fidelity.

56 ight unnatural amino acids in response to an amber codon with high yields and fidelities.

57 creasing the efficiency of suppression at an amber codon within the gene.

58 tigate the means by which suppression of the amber codon within these genes occurs, MtmB, a methyltra