ライフサイエンスコーパス: amino acid sequencing

1 hy, SDS-PAGE, Western blotting analysis, and amino acid sequencing.

2 residues of pro-IL-16 by immunoblotting and amino acid sequencing.

3 no acid preferences were determined by Edman amino acid sequencing.

4 ng peptide fragments were analyzed by direct amino acid sequencing.

5 recently determined for this glycoprotein by amino acid sequencing.

6 ectrospray mass spectrometry, and N-terminal amino acid sequencing.

7 steps were isolated and subjected to partial amino acid sequencing.

8 se chromatography and directly identified by amino acid sequencing.

9 and its glycosylation pattern determined by amino acid sequencing.

10 f the proteins was subsequently confirmed by amino acid sequencing.

11 identification of several unique peptides by amino acid sequencing.

12 e of [14C]NEM incorporation was subjected to amino acid sequencing.

13 the AE2 transmembrane domain were defined by amino acid sequencing.

14 n) in solution were determined by N-terminal amino acid sequencing.

15 the bd oxidase were identified by N-terminal amino acid sequencing.

16 d C18 column chromatography and subjected to amino acid sequencing.

17 rophoresis and subjected to partial internal amino acid sequencing.

18 e basis of specific antibody recognition and amino acid sequencing.

19 uencing data and the results from N-terminal amino acid sequencing.

20 urce Q; Amersham Pharmacia) and subjected to amino acid sequencing.

21 The isolated proteins are identified by amino acid sequencing.

22 -gel electrophoresis, mass spectrometry, and amino acid sequencing.

23 lected proteins were subjected to N-terminal amino acid sequencing.

24 BPAg2 is identical to LABD97 on the basis of amino acid sequencing.

25 d subjected to tryptic digestion and partial amino acid sequencing.

26 ted to a combination of manual and automated amino acid sequencing.

27 ourteen of these fractions were subjected to amino acid sequencing.

28 ree could be identified by Edman degradation amino-acid sequencing.

29 ism was purified and subjected to N-terminal amino-acid sequencing.

30 Partial amino acid sequencing (72% of the protein), mass spectra

31 By amino acid sequencing, about 35% of the peptides were de

32 Amino acid sequencing after cyanogen bromide cleavage yi

33 assisted laser-desorption mass spectroscopic amino acid sequencing after tryptic in-gel digestion and

34 N-terminal amino acid sequencing allowed for the synthesis of degen

35 Biochemical studies and amino acid sequencing analyses indicate that these intra

36 According to N-terminal amino acid sequencing and bioinformatic analyses, proven

37 le lysates, and its identity was verified by amino acid sequencing and by mass spectrometry.

38 membrane, bands of interest were analyzed by amino acid sequencing and by Western blotting.

39 Using immunoprecipitation, Western analysis, amino acid sequencing and end-point dilution analysis, w

40 Results of N-terminal amino acid sequencing and immunoblot analysis demonstrat

41 Amino acid sequencing and immunoblotting studies indicat

42 e examined the identity of Gbetah by partial amino acid sequencing and immunological characterization

43 N-terminal amino acid sequencing and immunoreactivity identified th

44 affinity chromatography and characterized by amino acid sequencing and inhibitor specificity analysis

45 trophoresis and identified by amino-terminal amino acid sequencing and interrogation of available dat

46 hin this fraction was achieved by N-terminal amino acid sequencing and liquid chromatography-mass spe

47 dentity of these fragments was determined by amino acid sequencing and MALDI mass spectrometry and re

48 ve molecular mass of 50 kDa, as predicted by amino acid sequencing and mass analysis, confirming that

49 ase chromatographic systems and subjected to amino acid sequencing and mass spectrometry analyses.

50 Amino acid sequencing and mass spectrometry analysis of

51 Amino acid sequencing and mass spectrometry indicated th

52 N-terminal amino acid sequencing and mass spectrometry indicated th

53 As determined by N-terminal amino acid sequencing and mass spectrometry of the cleav

54 Using amino acid sequencing and mass spectrometry, we determin

55 70(3-4)) were identified by a combination of amino acid sequencing and mass spectrometry.

56 C and [(14)C]DEPC modification, coupled with amino acid sequencing and matrix-assisted laser desorpti

57 ed to purify this growth factor, followed by amino acid sequencing and molecular cloning of the murin

58 Amino acid sequencing and more detailed structure elucid

59 was established to be at the correct site by amino acid sequencing and proceeded at approximately 11%

60 Amino acid sequencing and protein fragment reconstructio

61 Amino acid sequencing and subsequent isolation of its cD

62 NH2-terminal amino acid sequencing and Western analysis using monospe

63 D of fibrinogen, as confirmed by N-terminal amino acid sequencing and Western blot analysis with an

64 Its identity was confirmed by N-terminal amino-acid sequencing and matrix assisted laser desorpti

65 om bombardment-mass spectrometry, N-terminal amino acid sequencing, and alpha-mannosidase digestion d

66 erformance liquid chromatography, N-terminal amino acid sequencing, and ES-QToF mass spectrometry.

67 sphorylation site was isolated, subjected to amino acid sequencing, and found to be phosphorylated on

68 lyacrylamide gel electrophoresis, N-terminal amino acid sequencing, and gel filtration chromatography

69 ld of amino acids in both subunits, shown by amino acid sequencing, and lability of the DCI-protein b

70 luding total amino acid composition, partial amino acid sequencing, and the adoption of alpha-helical

71 isolated protein was subjected to N-terminal amino acid sequencing, and the derived amino acid sequen

72 All peptides identified by amino acid sequencing are encoded by the cDNA.

73 protein in the 73-kDa band was identified by amino acid sequencing as prolylcarboxypeptidase (PRCP).

74 or site of phosphorylation was identified by amino acid sequencing as Ser505.

75 A protein from that area was identified, by amino acid sequencing, as a homologue of Sec31 from Sacc

76 the N-terminus of proteins is important for amino acid sequencing by Edman degradation, protein iden

77 y purification of biotinylated-peptides, and amino acid sequencing by liquid chromatography tandem MS

78 as made and confirmed through amino-terminal amino acid sequencing by locating sites of derivatizatio

79 n of MGAD in which HSC70, as identified from amino acid sequencing, co-purified with GAD.

80 Amino acid sequencing confirmed that the 88-kDa band con

81 amino acid sequence analysis and N-terminal amino acid sequencing data suggest that LytM is a secret

82 s screened using probes based on the peptide amino acid sequencing data.

83 one-dimensional peptide mapping, and partial amino acid sequencing demonstrated regions of the VmpA p

84 N-terminal amino acid sequencing demonstrated that it corresponds t

85 ase analysis, site-directed mutagenesis, and amino acid sequencing demonstrated that maturation to th

86 initiator UUG codon, confirmed by N-terminal amino acid sequencing, encoding a 70,996-Da protein with

87 x-ray crystallographic, mass spectrometric, amino acid sequencing, enzymatic, and cellular data to s

88 ions, deletional mutagenesis, and N-terminal amino acid sequencing established that the signal for Hi

89 Amino acid sequencing failed to show the tyrosine residu

90 N-terminal amino acid sequencing (first 15 amino acids) and Western

91 in as the actin- binding protein gelsolin by amino acid sequencing following peptide mapping.

92 d selected antigens to mass spectroscopy for amino acid sequencing for comparison against a B. hermsi

93 Amino acid sequencing identified it as dihydrolipoamide

94 ptic peptide with V8 protease and N-terminal amino acid sequencing identified the modified residue as

95 N-terminal amino acid sequencing identified the peptide (V(545)LGFC

96 blotting, immunoprecipitation and N-terminal amino acid sequencing identified, in the early media fro

97 ed by MALDI mass spectrometry and N-terminal amino acid sequencing, identified 31 distinct polypeptid

98 N-terminal and internal amino acid sequencing indicated that I2PP2A was a trunca

99 Amino acid sequencing indicated that protein was related

100 Amino acid sequencing indicated that SP85 and SP35 are e

101 rylamide gel electrophoresis, and N-terminal amino acid sequencing indicated that the native lectin,

102 Amino acid sequencing indicates that protein cleavage oc

103 N-terminal amino acid sequencing of a 16-kDa Lys-C proteolytic frag

104 ntatively identified at the protein level by amino acid sequencing of a 7-kD cysteine-rich polypeptid

105 tive two-dimensional gel electrophoresis and amino acid sequencing of a peptide fragment of the pp3 p

106 NH2-terminal amino acid sequencing of a purified rabbit CE allowed th

107 known CE or other junctional proteins and 2) amino acid sequencing of cross-linked peptides derived b

108 Amino-terminal amino acid sequencing of highly purified p39 revealed ab

109 Amino-terminal amino acid sequencing of histidine-tagged Hap(beta) spec

110 Tryptic mapping and amino acid sequencing of in vitro autophosphorylated typ

111 Amino acid sequencing of isolated amyloid protein identi

112 Amino acid sequencing of natural Bla g 1 and Per a 1 sug

113 ed from p40-T1A-1 by proteolysis, N-terminal amino acid sequencing of p15-TIA-1 immunoaffinity purifi

114 otein was isolated from both cell types, and amino acid sequencing of peptide fragments identified th

115 Amino acid sequencing of purified IGFBP-5 fragments sugg

116 In the present study, by amino acid sequencing of recombinant IA-2 and IA-2 beta,

117 Amino acid sequencing of the 100-kDa protein showed homo

118 Amino acid sequencing of the 150-kDa band revealed seque

119 Direct amino acid sequencing of the 20 N-terminal amino acids c

120 Amino acid sequencing of the fragments deriving from cle

121 Amino-terminal amino acid sequencing of the heavy chain cleavage produc

122 al application of this method for subsequent amino acid sequencing of the isolated phosphopeptides wa

123 This was achieved by Edman amino acid sequencing of the isolated tandem repeat afte

124 Purification and partial amino acid sequencing of the mammalian rsec6/8 complex r

125 By amino acid sequencing of the N terminus of R.MamI and co

126 th that deduced from both cDNA, and previous amino acid sequencing of the protein isolated from chick

127 N-terminal amino acid sequencing of the purified protein revealed t

128 Amino acid sequencing of the purified protein showed str

129 nt molecular mass of 67 kDa after N-terminal amino acid sequencing of the purified protein.

130 N-terminal amino acid sequencing of the recombinant protein yielded

131 Amino acid sequencing of the second cobalt-containing pe

132 BglII, SfiI, and BssSI are proposed based on amino acid sequencing of their Fe2+/ascorbate-generated

133 Amino acid sequencing of these subunit polypeptides reve

134 Partial amino acid sequencing of this 38-kDa protein has indicat

135 Amino-terminal amino acid sequencing of this peptide from recombinant h

136 Amino acid sequencing of tryptic peptides from a 34-kDa

137 Amino acid sequencing of VWF fragments produced by InhA

138 Following partial amino-acid sequencing of one of these pollen coat protei

139 tified using the information from N-terminal amino acid sequencing, reactivity to antibodies, apparen

140 Amino acid sequencing revealed a double sequence corresp

141 Amino acid sequencing revealed a number of potential pho

142 Amino acid sequencing revealed it to be a member of the

143 Subsequent amino acid sequencing revealed many peptides involving i

144 NH(2)-terminal amino acid sequencing revealed near 50% identity with ra

145 preparative gel electrophoresis; N-terminal amino acid sequencing revealed substantial homology with

146 NH(2)-terminal amino acid sequencing revealed that MMP-12 cleaved TFPI

147 Partial amino acid sequencing revealed that one of the RNA bindi

148 N-terminal amino acid sequencing revealed that the 61-kDa antigen h

149 Chromatographic purification and amino acid sequencing revealed that the protein was pros

150 mbination of mass spectrometric analysis and amino acid sequencing revealed that tyrosine 204 was cro

151 Amino acid sequencing revealed the N-terminal sequence E

152 Western blotting and amino acid sequencing revealed the presence of abundant

153 Partial amino acid sequencing reveals that the Cat-307 protein i

154 next identified and cloned by using partial amino acid sequencing, reverse transcriptase PCR, and a

155 of Phe300 by MALDI-TOF mass spectrometry and amino acid sequencing showed that hydroxylation only occ

156 Amino acid sequencing suggests that the second cytochrom

157 Immunoprecipitation and amino acid sequencing techniques identified manganese su

158 P to homogeneity and established by means of amino-acid sequencing that it is a mixture of several 14

159 digestion followed by mass spectrometry and amino acid sequencing, the aziridine-containing ligand i

160 nano-electrospray tandem mass spectrometery amino acid sequencing, the levels of immunodetection of

161 From N-terminal amino acid sequencing, the PufX protein of Rb. capsulatu

162 roteolytic digestion, mass spectrometry, and amino acid sequencing, the remaining intrachain disulfid

163 By means of radiochemical amino acid sequencing, the site in the polyprotein cleav

164 Amino acid sequencing then showed that this effect invol

165 es containing carbohydrate were subjected to amino acid sequencing to identify the specific Asn resid

166 itive methods permit recovery and sufficient amino acid sequencing to identify these proteins.

167 We also show, using N-terminal amino acid sequencing together with analysis of the dist

168 Automated amino acid sequencing using Edman degradative chemistry

169 N-terminal amino acid sequencing verified that the N-terminal prima

170 N-terminal amino acid sequencing verified the Western blot and ELIS

171 By using phosphopeptide mapping and amino acid sequencing, we identified PKA phosphorylation

172 The protein was identified by amino acid sequencing, Western blotting, and immunohisto

173 a purity of >90% and subjected to N-terminal amino acid sequencing, which demonstrated that the 42-kD

174 of the bovine mature N-termini determined by amino acid sequencing with the deduced mammalian MRP seq