ライフサイエンスコーパス: aminoacylate

1 ed, mature tRNA(Arg)(CCG) is not efficiently aminoacylated.

2 increasing the fraction of tRNA that can be aminoacylated.

3 te amino acid-specific editing but cannot be aminoacylated.

4 e pairs, since the A29:U41 mutant is readily aminoacylated.

5 n cells was transported to the cytoplasm and aminoacylated.

6 e mitochondrial-encoded tyrosyl-tRNA that it aminoacylates.

7 n, tmRNAala enters the ribosome and adds its aminoacylated alanine to the nascent polypeptide.

8 We show that an aminoacylated amber suppressor tRNA (supF) derived from

9 The resulting aminoacyl-tRNA synthetases aminoacylate an amber suppressor tRNA with a desired unn

10 The mutant tRNAs are fully aminoacylated and do not interfere with the translation

11 tionship between the ability of a tRNA to be aminoacylated and its ability to stimulate the editing a

12 The mutant tRNAs were not aminoacylated, and the levels of complementation were lo

13 ntact and truncated cross-linked tmRNAs were aminoacylated as efficiently as the respective nonirradi

14 pliced tRNAs, regardless of whether they are aminoacylated, assemble into Los1-RanGTP complexes, docu

15 triplet and may, therefore, be inefficiently aminoacylated because of a lack of anticodon-triggered a

16 l ProRS enzymes possess the dual capacity to aminoacylate both tRNA(Pro) and tRNA(Cys) with their cog

17 nating glutamyl-tRNA synthetase (GluRS) that aminoacylates both tRNA(Gln) and tRNA(Glu) with glutamat

18 RNAs in vivo show that the initiator tRNA is aminoacylated but is not formylated in H. volcanii.

19 d by the m.8344A>G mutation in mt-tRNA(Lys), aminoacylated by a Class II aaRS.

20 onsense codons permits tRNA (Trp)(CCA) to be aminoacylated by A.thaliana lysyl-tRNA synthetase.

21 Here we show that an RNA minihelix was aminoacylated by an aminoacyl-phosphate-D-oligonucleotid

22 n vivo are (i) a suppressor tRNA that is not aminoacylated by any of the endogenous aminoacyl-tRNA sy

23 cating that none of the suppressor tRNAs are aminoacylated by any of the twenty aminoacyl-tRNA synthe

24 tions in the mt-tRNA(Ile), both of which are aminoacylated by Class I mt-aminoacyl-tRNA synthetases (

25 r-TpsiC helix of tRNA(Ile) or tRNA(Val) were aminoacylated by cognate synthetases selectively with is

26 duced a tRNA substrate which was efficiently aminoacylated by CysRS, even though the tertiary core re

27 tRNA(Leu(UUR)), and tRNA variants that were aminoacylated by hs mt LeuRS were isolated using an in v

28 f the next module, SrfB2, was not detectably aminoacylated by SrfB1, indicative of protein-protein re

29 RNA(Pro), which lacks these elements, is not aminoacylated by the bacterial enzyme.

30 are based on the acceptor stem alone can be aminoacylated by the class I methionyl-tRNA synthetase.

31 d human tRNACys to ones that are efficiently aminoacylated by the E. coli enzyme, we have identified

32 ain of human tRNA(Lys,3)was not specifically aminoacylated by the human enzyme.

33 a defect in the ability of mutant tRNA to be aminoacylated by the human mitochondrial leucyl-tRNA syn

34 vious studies reported that tRNAPyl could be aminoacylated by the synthetase-like protein PylS.

35 n a system in which the same tRNA species is aminoacylated by two unrelated synthetases.

36 Transfer RNA (tRNA) decodes mRNA codons when aminoacylated (charged) with an amino acid at its 3' end

37 Lysyl-tRNA synthetase aminoacylates CoA-SH with lysine, leucine, threonine, al

38 Here I show that isoleucyl-tRNA synthetase aminoacylates CoA-SH with valine, leucine, threonine, al

39 re closely related in evolution and yet they aminoacylate contrasting tRNAs.

40 ranslational defects and thereby reduced the aminoacylated efficiencies of tRNA(Leu(UUR)) as well as

41 The aminoacylated efficiency and steady-state level of tRNA(

42 ryotic elongation factors eEF1A and eEF2 and aminoacylated elongator tRNAs resulted in the synthesis

43 eductions in the steady-state levels and the aminoacylated fraction of tRNA(Leu(UUR)) are likely to c

44 The alpha-NH(2) group of an aminoacylated fragment in the A site forms one hydrogen

45 Aminoacylated fully modified tRNAs and unmodified tRNA t

46 two GluRS enzymes, with GluRS2 specifically aminoacylating Glu onto tRNA(Gln).

47 o-step pathway for the specific synthesis of aminoacylated glutamine and/or asparagine tRNAs, involvi

48 hondrial phenylalanyl-tRNA synthetase, which aminoacylates hmt-tRNA(Phe) with cognate phenylalanine.

49 indicate that human tyrosyl-tRNA synthetase aminoacylates human but not B. stearothermophilus tRNATy

50 pecific tRNA (tRNA(cys)) was transcribed and aminoacylated in a single reaction.

51 We show that the tRNA is not aminoacylated in COS-1 cells by any of the endogenous am

52 . coli tRNA3Lys expressed from a plasmid was aminoacylated in mammalian cells.

53 All tRNAs examined can be aminoacylated in nuclei of Xenopus oocytes, thereby prov

54 We now show that tRNAPyl is efficiently aminoacylated in the presence of both the class I LysRS

55 This mutant initiator tRNA can, however, be aminoacylated in vitro by the Escherichia coli glutaminy

56 rified after expression in Escherichia coli, aminoacylated in vitro transcripts corresponding to both

57 tRNA(Leu(UUR)) was 25-fold less efficiently aminoacylated in vitro, compared to native wild-type tRN

58 The suppressor tRNAs are aminoacylated in vivo only in the presence of the hetero

59 oacyl-tRNA synthetases, the mutant tRNAs are aminoacylated in vivo with different amino acids.

60 Here we show that the Mi:2 tRNA is normally aminoacylated in vivo with lysine and that the tRNA amin

61 tiation begins with ribosomal recruitment of aminoacylated initiator tRNA (Met-tRNA(Met)(i)) by eukar

62 ted intermediate requires the presence of an aminoacylated initiator, fMet-tRNA(fMet), and IF2 in the

63 ular permeability to cationic antibiotics by aminoacylating inner membrane lipids.

64 followed by transient formation of the alpha-aminoacylate intermediate with a slightly lower rate (70

65 he small fraction of hmtRNA(Met) that can be aminoacylated is not formylated by the mitochondrial Met

66 RS) is first evolved in yeast to selectively aminoacylate its tRNA with the unnatural amino acid of i

67 he A/PCP 105-kDa fragment of Lys2 covalently aminoacylated itself with [35S]S-carboxymethyl-L-cystein

68 However, approximately 60% increase in aminoacylated level of tRNAHis was observed in mutant ce

69 ed by the fact that the bacterial synthetase aminoacylates mammalian initiator tRNA, but not elongato

70 ycotina fungi are bifunctional proteins that aminoacylate mitochondrial tRNA(Tyr) and are structure-s

71 yrosyl-tRNA synthetase (CYT-18 protein) both aminoacylates mitochondrial tRNA(Tyr) and acts as a stru

72 se (TyrRS; CYT-18), is bifunctional and both aminoacylates mitochondrial tRNA(Tyr) and promotes the s

73 side chain, whereas the other recognizes an aminoacylated oligonucleotide.

74 mino acids in nature and their ability to be aminoacylated onto tRNAs by aa-tRNA synthetases.

75 y favors the use of a class I-type enzyme to aminoacylate particular tRNALys species and provides a m

76 Although both forms of MprF aminoacylate PG, they do so with different amino acids;

77 dditions to mt-tRNA led to reduced levels of aminoacylated pool of certain mt-tRNAs and mitoribosome

78 The enzyme is unable to aminoacylate purified mature M. jannaschii tRNA(Cys) wit

79 ealed a variable decrease in mobility of the aminoacylated relative to the nonacylated form, with the

80 Ribozymes that aminoacylate RNA by using activated amino acids have bee

81 urther imply that early life consisted of an aminoacylated RNA world with a richer enzymatic potentia

82 d that the class I methionyl-tRNA synthetase aminoacylates RNA microhelices based on the acceptor ste

83 A self-aminoacylating RNA catalyst is shown to carry out the ch

84 Comparison of six independent self-aminoacylating RNAs derived from selection-amplification

85 Aminoacylated-S-CmaE will transfer the l-Val moiety to t

86 otic elongation factor (eEFSec) delivers the aminoacylated selenocysteine-tRNA (Sec-tRNA(Sec)) to the

87 synthetase (GlnRS) proteins that incorrectly aminoacylate the amber suppressor derived from tRNATyr (

88 Moreover, the 5'-leader segment is able to aminoacylate the mature tRNA in trans.

89 These mutants still aminoacylate the suppressor tRNA essentially quantitativ

90 three molecules of L-cysteine and covalently aminoacylates the phosphopantetheinyl (P-pant) thiols on

91 ) and (ii) an aminoacyl-tRNA synthetase that aminoacylates the suppressor tRNA but no other tRNA in t

92 he hydroxyl groups of a tRNA, it exclusively aminoacylates the terminal 3'-OH.

93 yl-tRNA synthetase was evolved that uniquely aminoacylates the unnatural amino acid onto an orthogona

94 Importantly, S. cerevisiae GlnRS aminoacylates the yeast orthogonal tRNA in vitro and in

95 recognize either proline or cysteine and can aminoacylate their cognate tRNAs through a dual-specific

96 the smallest tRNA(leu) analog that has been aminoacylated to a significant extent to date.

97 The CAU-->CUA mutant was not aminoacylated to any significant extent in vivo, suggest

98 (Met) residues used in protein synthesis are aminoacylated to non-methionyl-tRNAs.

99 of 32P-labeled pyrophosphate into ATP and to aminoacylate total E. coli tRNA with L-serine.

100 ia coli translation system with specifically aminoacylated total tRNA that has been chemically methyl

101 G or elongation factor Tu complexed with an aminoacylated transfer RNA and GTP onto the factor-bindi

102 HisRS homologs that, like HisZ, cannot aminoacylate tRNA are represented in a highly divergent

103 h Escherichia coli ThrRS, which is unable to aminoacylate tRNA(1Thr), reveals differences in the anti

104 gent pattern of conservation in enzymes that aminoacylate tRNA(Glu)versus those specific for tRNA(Gln

105 NA synthetase whose essential function is to aminoacylate tRNA(Ile) with isoleucine.

106 c class IIb KRS, including the propensity to aminoacylate tRNA(Lys) with suboptimal identity elements

107 LysRS (S207D) that lacked the capability to aminoacylate tRNA(Lys3) localized to the nucleus, rescue

108 istidyl-tRNA synthetase (HisRS) but does not aminoacylate tRNA.

109 ext of the kinetic proofreading mechanism of aminoacylated tRNA (aa-tRNA) selection.

110 denosine towards a beta-strand, such that an aminoacylated tRNA at this position would be sterically

111 loped that directly measures the fraction of aminoacylated tRNA by following amino acid attachment to

112 e or puromycin, which inhibit utilization of aminoacylated tRNA in cells; and 3) in cells having a te

113 In the other conformation, presumptive aminoacylated tRNA is bound only by the anticodon, the a

114 cating that much of the binding affinity for aminoacylated tRNA is derived from interaction with the

115 In addition to raising aminoacylated tRNA levels, the absence of editing lowere

116 cid starvation is sensed by depletion of the aminoacylated tRNA pools, and this results in accumulati

117 the repair of the genome, while ARSs provide aminoacylated tRNA precursors for protein synthesis.

118 An aminoacylated tRNA was synthesized which was complementa

119 a strong (70-75%) reduction in the level of aminoacylated tRNA(Leu(UUR)) and a decrease in mitochond

120 To determine if the decreased fraction of aminoacylated tRNA(Leu(UUR)) in A3243G mutant cells was

121 lso results in a decrease in the fraction of aminoacylated tRNA(Leu(UUR)).

122 diting-defective strain, increased levels of aminoacylated tRNA(Phe) led to continued synthesis of th

123 n the absence of editing, cellular levels of aminoacylated tRNA(Phe) were elevated during amino acid

124 aternary complex consisting of Msn5, RanGTP, aminoacylated tRNA, and Tef1/2.

125 ongation factors 1A and 2 and A site-cognate aminoacylated tRNA.

126 contrast, previous work has shown that CysRS aminoacylates tRNA(Cys) core regions containing G15-G48

127 e prolyl-tRNA synthetase also recognizes and aminoacylates tRNA(Cys) with cysteine.

128 Like a canonical GluRS, GluRS1 aminoacylates tRNA(Glu1) and tRNA(Glu2).

129 luRS2), which specifically and more robustly aminoacylates tRNA(Glu1) instead of tRNA(Gln).

130 sual tRNA substrate specificity, efficiently aminoacylating tRNA(His) regardless of the presence of G

131 in splicing group I introns, in addition to aminoacylating tRNA(Tyr).

132 actor 1A (eEF1A; EF-Tu in bacteria) delivers aminoacylated-tRNA to the A-site of the ribosome, wherea

133 Alanyl-tRNA synthetase efficiently aminoacylates tRNAAla and an RNA minihelix that comprise

134 netic code are established in reactions that aminoacylate tRNAs with specific amino acids.

135 a 2.3- to 4.2-fold decrease in the level of aminoacylated tRNAs and a >2-fold decrease in growth rat

136 presence of the heterologous aaRSs, and the aminoacylated tRNAs function efficiently in suppression

137 stalled ribosomes and discriminates against aminoacylated tRNAs is missing.

138 ha [eEF1A]) aids the specificity of Msn5 for aminoacylated tRNAs to form a quaternary complex consist

139 (aaPGSs) are membrane proteins that utilize aminoacylated tRNAs to modify membrane lipids with amino

140 ion and dissociation rates of deacylated and aminoacylated tRNAs to the A-site and P-site of E. coli

141 thereby facilitating efficient re-export of aminoacylated tRNAs to the cytoplasm.

142 ng to dramatic reductions in the fraction of aminoacylated tRNAs, cessation of protein synthesis and

143 rentially assembles with RanGTP and spliced, aminoacylated tRNAs, documenting its role in tRNA nuclea

144 ained limited primarily to canonical RNA, 3'-aminoacylated tRNAs, nucleobase-modified RNAs, and 5'-ca

145 on of amino acids via synthetically prepared aminoacylated tRNAs.

146 ranslational resources, namely ribosomes and aminoacylated tRNAs.

147 eukaryotic elongation factors 1A and 2, and aminoacylated tRNAs.

148 ed by 70S ribosomes programmed with mRNA and aminoacylated tRNAs.

149 mechanisms have recently been described for aminoacylating tRNAs with asparagine, cysteine, glutamin

150 es perform a critical step in translation by aminoacylating tRNAs with their cognate amino acids.

151 icipate in protein translation by presenting aminoacylated-tRNAs to the ribosome.

152 ngle aminoacyl-tRNA synthetase (aaRS), MST1, aminoacylates two isoacceptor tRNAs, tRNA1(Thr) and tRNA

153 evisiae, a single aaRS (MST1) recognizes and aminoacylates two natural tRNAs that contain anticodon l

154 n accumulation of ribosomes stalled with non-aminoacylated (uncharged) tRNA in the ribosomal A site.

155 herichia coli leucyl-tRNA synthetase (LeuRS) aminoacylates up to six different class II tRNA(leu) mol

156 The cys-tRNA(cys) that was synthesized and aminoacylated using this method was functional in in vit

157 he)UUA) that did not have the capacity to be aminoacylated was transported to the cytoplasm and did c

158 ld-type and mutant human initiator tRNAs are aminoacylated well in vivo.

159 f a variety of tRNA transcripts which can be aminoacylated well.

160 e various constructs that were significantly aminoacylated were also tested for amino acid editing by

161 This tRNA was also aminoacylated when expressed in mammalian cells and comp

162 show only small changes in their ability to aminoacylate wild-type cognate tRNA on the one hand and

163 r (CUA) initiator suppressor tRNA chemically aminoacylated with a fluorophore-amino acid conjugate wh

164 open the possibility of using the supF tRNA aminoacylated with an amino acid analogue as a general a

165 not the holo-PKS module was then selectively aminoacylated with cysteine by the adenylation domain em

166 g the activity in initiation of mutant tRNAs aminoacylated with glutamine and valine.

167 The mutant tRNAs used are aminoacylated with glutamine, methionine, and valine.

168 steps limiting the activity of mutant tRNAs aminoacylated with isoleucine and phenylalanine.

169 tic systems, however, a yeast initiator tRNA aminoacylated with isoleucine was found to be inactive i

170 e find that holo-CepA1-575 can be covalently aminoacylated with l-leucine on the peptidyl carrier pro

171 pts to construct a minihelix RNA that can be aminoacylated with leucine have been unsuccessful.

172 The minimal RNA that was efficiently aminoacylated with LeuRS was one in which the anticodon

173 ylated in vivo with lysine and that the tRNA aminoacylated with lysine is a very poor substrate for f

174 We showed previously that a mutant tRNA aminoacylated with lysine was an extremely poor substrat

175 or the formylation defect of the mutant tRNA aminoacylated with lysine.

176 s active in initiation in vivo but only when aminoacylated with methionine by overproduction of methi

177 In contrast, the same tRNA, when aminoacylated with methionine, was a good substrate for

178 for formylation compared with the same tRNA aminoacylated with methionine.

179 two-step pathway in which tRNA(Cys) is first aminoacylated with phosphoserine by phosphoseryl-tRNA sy

180 product, an amber-decoding tRNA(Pyl) that is aminoacylated with pyrrolysine by the pyrrolysyl-tRNA sy

181 tRNA(Sec) is initially aminoacylated with serine by seryl-tRNA synthetase and t

182 RNA population in Drosophila melanogaster is aminoacylated with serine, forms selenocysteyl-tRNA, and

183 s that have complementary UCA anticodons are aminoacylated with serine, the seryl-tRNA is converted t

184 e the import into cells of a suppressor tRNA aminoacylated with the analogue of choice.

185 ability of 24 mutant tRNA(Pyl) species to be aminoacylated with the pyrrolysine analog N-epsilon-cycl

186 he CAU-->GAC anticodon mutant is most likely aminoacylated with valine in vivo.

187 .Bacterial glutaminyl-tRNA synthetase poorly aminoacylates yeast tRNA and, as a consequence, cannot r