ライフサイエンスコーパス: amphotropic

1 ntry: the ecotropic, xenotropic, polytropic, amphotropic, 10A1, and Mus dunni endogenous virus groups

2 tropic Moloney murine leukemia virus and the amphotropic 4070A isolate.

3 ent on the presence of the C terminus of the amphotropic 4070A SU protein.

4 f CHO/rRAM-1 cells, syncytia form at foci of amphotropic 4070A virus infection by fusion-from-within

5 v) were generated in which the N terminus of amphotropic 4070A was replaced by equivalent sequences f

6 ted between the ecotropic (Moloney-MuLV) and amphotropic (4070A) SU and TM proteins of murine leukemi

7 Ecotropic, amphotropic (4070A), and amphotropic-mink cell focus-for

8 enotropic and polytropic viruses and between amphotropic and 10A1 viruses, indicating some overlap in

9 leukosis virus-based retroviral vectors with amphotropic and ecotropic host ranges.

10 and that the infectious process of both the amphotropic and ecotropic MuLVs very likely involved end

11 ells are naturally resistant to infection by amphotropic and ecotropic murine retroviruses, but they

12 aneous infection with vectors targeting both amphotropic and GALV receptors may prove to be of additi

13 is present study, we examined the ability of amphotropic and pseudotyped vectors expressing a murine

14 e RBD from Fr-MLV also restores infection by amphotropic and xenotropic MLVs in which RBD is deleted.

15 odified ASV encoding a murine leukemia virus amphotropic env gene and a green fluorescent protein (GF

16 ile an otherwise identical vector bearing an amphotropic Env is inactivated.

17 inant, designated MoLTRAmphoenv in which the amphotropic env of the helper packaging virus was joined

18 opic Envs (CasBrE and Friend), but not 4070A amphotropic Env.

19 emia virus envelope (PG13 cells), the murine amphotropic envelope (FLYA13 cells), or the feline endog

20 virus coated with the murine leukemia virus amphotropic envelope glycoprotein, containing either wil

21 iral vectors has been developed in which the amphotropic envelope is completely replaced by the G gly

22 pressive activity is not associated with the amphotropic envelope or the glyco-Gag protein.

23 ar stomatitis virus glycoprotein or with the amphotropic envelope protein of murine leukemia virus.

24 protein that joins the amino terminus of the amphotropic envelope protein to the Fc region of a human

25 Limited expression of the amphotropic envelope receptor is a recognized barrier to

26 ies showed that when SCF was displayed on an amphotropic envelope, the infectivity of the SCF-display

27 cells with a greater efficiency than similar amphotropic envelope-pseudotyped vectors.

28 e efficiently than a similar vector with the amphotropic envelope.

29 V-1 vector than with a vector packaged in an amphotropic envelope.

30 HIV envelopes and the murine leukemia virus amphotropic envelope.

31 ffect on the entry of virus pseudotyped with amphotropic envelope.

32 Amphotropic-envelope-pseudotyped virus stocks prepared w

33 d HERA, which code for the ecotropic and the amphotropic envelopes, respectively.

34 Cs) with retroviral vectors pseudotyped with amphotropic envelopes.

35 from four unrelated FA-A patients, using two amphotropic FAA retroviral vectors.

36 C-SIN amphotropic G418-resistant virus particles were generate

37 s of the effusions and was demonstrated with amphotropic, gibbon ape leukemia virus, and vesicular st

38 ing the gamma(c) gene IL2RG pseudotyped with amphotropic, gibbon ape leukemia virus, or RD114 envelop

39 the CNS using either the "non-neurovirulent" amphotropic helper virus, 4070A, or pgag-polgpt (a nonpa

40 n ecotropic host range, and six exhibited an amphotropic host range and viral interference.

41 In previous studies amphotropic MFGS-gp91phox (murine onco-retrovirus vector

42 Ecotropic, amphotropic (4070A), and amphotropic-mink cell focus-forming hybrid (10A1) envelo

43 culated neonates with Moloney MLV (MoMLV) or amphotropic MLV (A-MLV) and screened for viral recombina

44 rast, infection of mink cells with the 4070A amphotropic MLV did not produce any cytopathic effects.

45 encapsidating cell line, which expresses the amphotropic MLV envelope and a retroviral vector (pGA-1)

46 e (ANGIE P) which is designed to express the amphotropic MLV envelope.

47 exhibited efficient transfection similar to amphotropic MLV vectors.

48 s was 27% compared with 2 to 3% for mock- or amphotropic MLV-infected cells, representing a 9- to 14-

49 vector RCASBP(A) with the env region from an amphotropic MLV.

50 esistance to infection by MCF MLV but not by amphotropic MLV.

51 fficiency of infection by ecotropic, but not amphotropic, MLV reporters.

52 ter superinfection with either xenotropic or amphotropic MLVs, these cells released HIV-gpt virions p

53 rticles carrying either the ecotropic or the amphotropic Mo-MLV envelope proteins or the vesicular st

54 d to the cytoplasmic domain derived from the amphotropic Moloney murine leukemia virus 4070A GP, reve

55 trovirus isolated from a mouse infected with amphotropic MuLV (A-MuLV).

56 the expression of the human receptor for the amphotropic MuLV (GLVR-2, newly termed Pit2), we determi

57 enomenon is observed with both ecotropic and amphotropic MuLV Env.

58 Basic knowledge of amphotropic MuLV receptor expression is likely to be use

59 MuLV-infected producer cells or of the PA317 amphotropic MuLV-based vector packaging line (also gPr80

60 hesus cell types compared with the prototype amphotropic MuLV4070A.

61 amined the infectious entry of ecotropic and amphotropic MuLVs and found that they were equally inhib

62 e infectious processes of both ecotropic and amphotropic MuLVs were arrested rather than aborted by t

63 eline leukemia virus subgroup B (FeLV-B) and amphotropic murine leukemia virus (A-MLV) are highly rel

64 eline leukemia virus subgroup B (FeLV-B) and amphotropic murine leukemia virus (A-MLV) have closely r

65 ions by gibbon ape leukemia virus (GALV) and amphotropic murine leukemia virus (A-MLV) unless they ar

66 stoichiometry of envelope glycoproteins from amphotropic murine leukemia virus (A-MLV), avian sarcoma

67 For some viruses, such as amphotropic murine leukemia virus (A-MLV), particles wit

68 OK1 dramatically differ in susceptibility to amphotropic murine leukemia virus (A-MuLV) and gibbon ap

69 Amphotropic murine leukemia virus (A-MuLV) utilizes the

70 Amphotropic murine leukemia virus (A-MuLV) utilizes the

71 the gibbon ape leukemia virus (GALV) and the amphotropic murine leukemia virus (A-MuLV), termed Pit1

72 sphate cotransporter and as the receptor for amphotropic murine leukemia virus (A-MuLV).

73 with autologous CD34+ cells transduced with amphotropic murine leukemia virus (MLV)-derived retrovir

74 retroviral vector with the host range of the amphotropic murine leukemia virus (MLV).

75 Nearly all retroviruses, including amphotropic murine leukemia virus (MuLV) and human immun

76 The amphotropic murine leukemia virus (MuLV) can infect cell

77 For the amphotropic murine leukemia virus (MuLV), a 208-amino-ac

78 CASBP M2C (797-8), which replicate using the amphotropic murine leukemia virus 4070A Env protein (2).

79 pendent HIV Env, CXCR4-dependent HIV Env, or amphotropic murine leukemia virus Env).

80 stomatitis virus G protein and 5 x 10(8) for amphotropic murine leukemia virus envelope).

81 truncated HIV-1 envelope glycoproteins, the amphotropic murine leukemia virus envelope, or the vesic

82 otyped with vesicular stomatitis virus G and amphotropic murine leukemia virus envelopes were not inh

83 otyped with envelope glycoproteins of either amphotropic murine leukemia virus or vesicular stomatiti

84 nly the GALV receptor (MolPit1) but also the amphotropic murine leukemia virus receptor (MolPit2).

85 receptor protein, Pit2, which serves as the amphotropic murine leukemia virus receptor.

86 Furthermore, similar treatment of an amphotropic murine leukemia virus significantly allows i

87 is not restored by pseudotyping with Env of amphotropic murine leukemia virus, demonstrating that Cy

88 quent infection not only by GALV but also by amphotropic murine leukemia virus.

89 ed so that it uses the envelope gene from an amphotropic murine leukemia virus.

90 rted the unique properties of a receptor for amphotropic murine leukemia viruses (A-MuLVs) expressed

91 MA is abolished when cells are infected with amphotropic murine retrovirus (binds Pit-2 receptor) but

92 r the gibbon ape leukemia retrovirus and the amphotropic murine retrovirus serve normal cellular func

93 having an envelope protein (pseudotype) from amphotropic murine retrovirus with similar vectors havin

94 resistant to infection by both ecotropic and amphotropic murine viruses, as well as by human immunode

95 CD34(+)PBSCs) with lentivector-gp91(phox) or amphotropic oncoretrovirus MFGS-gp91(phox) and evaluated

96 cient mice, and retrovirally transduced with amphotropic or ecotropic vectors encoding a normal acid

97 cells producing equivalent titers of either amphotropic or GALV pseudotyped vectors containing the n

98 luciferase viruses complemented with either amphotropic or HIV-derived envelopes, we found a direct

99 y be advantageous compared with the standard amphotropic packaging cells because vectors produced by

100 or (tTA), was stably incorporated into PA317 amphotropic packaging cells, thus generating the packagi

101 ed with RD114-pseudotyped particles, whereas amphotropic particles were ineffective at introducing th

102 0 of 13 long-term repopulated animals, while amphotropic proviral sequences were detected in only one

103 ned ecotropic provirus and 6 of 11 contained amphotropic provirus sequences, a significant increase i

104 Despite this variability, the amphotropic PRR could functionally substitute for the ec

105 A single exposure to a low concentration of amphotropic pseudotyped SIV vector particles encoding th

106 (LTR) transactivation, and transduction with amphotropic-pseudotyped murine leukemia virus (MLV)-base

107 provement of gene therapy protocols based on amphotropic-pseudotyped vectors.

108 was demonstrated when vectors targeting the amphotropic receptor and the gibbon ape leukemia virus (

109 interference when two vectors targeting the amphotropic receptor are used simultaneously.

110 the AE4 chimeric Env backbone containing the amphotropic receptor binding domain joined at the hinge

111 ct point within VRA, and we suggest that the amphotropic receptor binding domain probably makes multi

112 evels of GALV receptor RNA compared with the amphotropic receptor in CD34(+) hematopoietic cells.

113 For example, the level of expression of the amphotropic receptor Pit-2, a phosphate symporter, appea

114 monstrate that the use of FN CH-296 leads to amphotropic receptor saturation in these cells.

115 0A (42 of processed SU) are not required for amphotropic receptor usage.

116 We transduced cells with amphotropic retroviral constructs containing SV40 T anti

117 e ferret cerebral cortex were tagged with an amphotropic retroviral library encoding alkaline phospha

118 s (FLC) despite resistance of these cells to amphotropic retroviral transduction (infection).

119 e colony-forming cells) could be shown after amphotropic retroviral transduction.

120 mediated gene transfer was studied using an amphotropic retroviral vector carrying the Escherichia c

121 An amphotropic retroviral vector encoding a bicistronic gen

122 beta-galactosidase-transducing ecotropic and amphotropic retroviral vector particles as a factor Xa p

123 An amphotropic retroviral vector with a liver-specific prom

124 We generated an amphotropic retroviral vector with the human FX cDNA and

125 al human thyrocytes in primary culture using amphotropic retroviral vectors and assessed met expressi

126 Currently, amphotropic retroviral vectors are widely used for gene

127 and PBL were separately transduced with the amphotropic retroviral vectors LNL6 and G1Na.

128 Human CEMSS T cells were transduced with amphotropic retroviral vectors to express RNA complement

129 An amphotropic retroviral-producing cell clone was created,

130 (IGF-I) as an N-terminal extension of 4070A (amphotropic) retroviral envelope protein.

131 tion of FLC in phosphate-free medium with an amphotropic retrovirus containing the multiple drug resi

132 cient CGD patient with replication defective amphotropic retrovirus encoding p67phox (MFGS-p67phox) s

133 Two significant barriers limit the use of amphotropic retrovirus for human gene transfer protocols

134 d by anion-exchange chromatography inhibited amphotropic retrovirus infection.

135 UACC-903(+6) cells, packaged into high-titer amphotropic retrovirus particles, and transduced into UA

136 Here we demonstrate that formation of amphotropic retrovirus polylysine molecular conjugates (

137 In the present study, we have examined amphotropic retrovirus receptor (amphoR) and ecotropic r

138 The highest levels of amphotropic retrovirus receptor (amphoR) mRNA were found

139 virus receptor levels; overexpression of the amphotropic retrovirus receptor Pit2 markedly improved t

140 eukemia virus receptor Glvr-1 (Pit-1) or the amphotropic retrovirus receptor Ram-1 (Pit-2).

141 Pretreatment of amphotropic retrovirus stocks with chondroitinase ABC bo

142 rus sequences, a significant increase in the amphotropic retrovirus transduction (P = 0.018).

143 ine-mobilized BM may be superior targets for amphotropic retrovirus transduction compared with HSC fr

144 the amphoR mRNA level in HSC correlates with amphotropic retrovirus transduction efficiency.

145 re analyzed simultaneously for ecotropic and amphotropic retrovirus transduction, 11 of 11 repopulate

146 re analyzed simultaneously for ecotropic and amphotropic retrovirus transduction, ecotropic provirus

147 8F rat embryo fibroblasts to an excess of an amphotropic retrovirus vector encoding alkaline phosphat

148 Thus, efficient transduction by an amphotropic retrovirus vector requires high-level expres

149 nhanced safety characteristics not only over amphotropic retrovirus vectors but also over genetically

150 The low level of amphotropic retrovirus-mediated gene transfer into human

151 embryonic fibroblasts were transformed using amphotropic retrovirus-transduced oncogenes (pBABE-c-Myc

152 mphoR mRNA is more efficiently transduced by amphotropic retrovirus.

153 uses (REVs) comprise several closely related amphotropic retroviruses isolated from birds.

154 ent phosphate transporter and a receptor for amphotropic retroviruses.

155 The amphotropic SU was unaffected by most of the point mutat

156 Concurrently, we substituted portions of the amphotropic SU with homologous regions from the polytrop

157 ate that (i) the normal resistance of FLC to amphotropic transduction is most likely due to an insuff

158 Spleen necrosis virus (SNV) is an amphotropic type C retrovirus originally isolated from a

159 A standard amphotropic vector expressing a serum marker protein, hu

160 ogenous virus (RD114) than with conventional amphotropic vector particles.

161 The hRGFP gene was used to produce an amphotropic vector producer cell line that demonstrated

162 The amphotropic vector RCASBP-M2C(797-8), was obtained by pa

163 ilized CD34(+) cells were transduced with an amphotropic vector that expressed EGFP and a dihydrofola

164 more efficient in infecting T cells than an amphotropic vector used at the same multiplicity of infe

165 beta-galactosidase-producing pseudotype and amphotropic vector.

166 rates of 2.7%, 7.1%, <15%, and 3.9% with the amphotropic vectors and 7.1%, 11.3%, <15%, and 26.4% wit

167 stant to human complement than commonly used amphotropic vectors and could be highly concentrated (>

168 icient at transducing murine stem cells than amphotropic vectors, and that among the three most commo

169 troviral transduction using ecotropic versus amphotropic vectors, various growth factor combinations,

170 ith the GALVpseudotyped vector than with the amphotropic vectors.

171 e used a murine retrovirus vector to produce amphotropic virions.

172 Amphotropic virus supernatants from stable producer cell

173 tion competent HIV-1-NL4-3-Env(-) luciferase amphotropic virus, which measures HIV-1 transcriptional

174 Toward this end, an amphotropic virus-producer cell line has been developed

175 cells, whereas no syncytia are reported for amphotropic virus.

176 herwise susceptible to infection with native amphotropic virus.

177 Ecotropic and amphotropic viruses differ in their ability to form larg

178 Entry assays employing amphotropic viruses show that MVN is inactive, whereas C