ライフサイエンスコーパス: amplification

1 ghput also but require high input amounts or amplification.

2 erent sides, as well as one-way displacement amplification.

3 erogeneity more effectively than chromosomal amplification.

4 itive status was defined as high polysomy or amplification.

5 PCR to guide the choice of protocols for SNP amplification.

6 dditionally, patients were assessed for 1q21 amplification.

7 associated with cell lysis and whole-genome amplification.

8 d fiberoptics conjugated with rolling circle amplification.

9 underwent multiplex ligation-dependent probe amplification.

10 g motifs to enable robust fluorescent signal amplification.

11 as signal molecular nanocarriers for signal amplification.

12 nonlinear hybridization chain reaction (HCR) amplification.

13 led in yeast and amplified by rolling circle amplification.

14 exploiting movement-triggered cascade signal amplification.

15 e DNA detection method that does not require amplification.

16 sion without substantial antecedent lymphoid amplification.

17 sign of target-specific primer pairs for DNA amplification.

18 CS level 2, active seizures, or hearing with amplification.

19 sensitization occurs through central signal amplification.

20 00 attomolar (aM) is achieved without target amplification.

21 nding in a process we call curvature cascade amplification.

22 We investigated whether MAF amplification (a biomarker for bone metastasis) in prima

23 that included a large region bearing complex amplifications, a growth defect mapping to a recoded seq

24 We present light amplification action in Rhodamine B doped silk fibroin (

25 genetic abnormalities and 172 (32%) had 1q21 amplification alone.

26 y and sensitive detection using nucleic acid amplification and analysis techniques.

27 We also demonstrate net optical amplification and Brillouin energy transfer as the basis

28 nds into multiple nanoliter compartments for amplification and construction of barcoded libraries for

29 s might employ similar mechanisms for signal amplification and dispersion.

30 tion probes relying solely on competition or amplification and enables SNV detection at 1% abundance.

31 in a fluorescence microscope upon isothermal amplification and fluorescence labeling.

32 HER2-positive breast cancers with HER2 gene amplification and HER2-low or HER2-negative breast cance

33 with a bridge linker, Tn5 tagmentation, PCR amplification and high-throughput sequencing.

34 re not without their shortcomings; enzymatic amplification and ligation of oligonucleotides into gene

35 those that prolong RNAi inheritance through amplification and maintenance of heritable small RNAs, a

36 e-tendon systems also affect its generation, amplification and maintenance.

37 could be further improved with more uniform amplification and more accurate sequence alignment.

38 synthesis (TLS) polymerase, followed by PCR amplification and next-generation sequencing (NGS) to ge

39 2 (HER2)-positive breast cancer, GSDMB gene amplification and protein overexpression indicate a poor

40 -lactide nanoparticles (PLLA NPs) for signal amplification and PSA detection.

41 we also found a clear connection between the amplification and quantum discord like measurements as w

42 ike RNAs were experimentally confirmed by re-amplification and re-sequencing, and have a similar qRT-

43 ncing and multiplex ligation-dependent probe amplification and retrospectively analyzed medical chart

44 High-risk NBL is characterized by N-Myc amplification and segmental chromosomal gains and losses

45 ory relating metabolic evolution to the self-amplification and self-organization of the biosphere.

46 asses, previously formally identified by PCR amplification and sequencing of internal transcribed spa

47 lar assay (ST Direct) relies on nucleic acid amplification and solid array-based amplicon detection t

48 cation complexes, which are required for the amplification and subsequent spread of the virus.

49 blasts lacking KIF1Bbeta independent of MYCN amplification and the loss of genes neighboring KIF1B on

50 ER2-positive with 1 BrM acquiring ERBB2/HER2 amplification and the other showing metastatic enrichmen

51 Fine-needle aspiration biopsy (FNAB) for DNA amplification and whole genome array-based assay were pe

52 ung children are usually associated with MYC amplification and/or overexpression, frequent metastasis

53 errant DNA methylation, abundant chromosomal amplifications and deletions, and mutational signatures

54 th TERT point mutations, rearrangements, DNA amplifications and transcript fusions and predictive of

55 f primer sets that correlate with successful amplification, and present guidelines for implementation

56 lated to conformational selection, signaling amplification, and probe dependence.

57 WNT activation, IGF2 gene overexpression and amplification; and (v) CRIS-E: Paneth cell-like phenotyp

58 ted isothermal amplification (LAMP) is a DNA amplification approach characterized by high sensitivity

59 enging both because of the lack of effective amplification approaches and the wide diversity in cellu

60 TATION: The exo-E415G SNP and plasmepsin 2-3 amplification are markers of piperaquine resistance and

61 hia coli, or Sae2 in eukaryotes, palindromic amplifications arise and propagate in cells.

62 n 50% examined transgenic cotton through PCR amplification assay and sequencing analyses.

63 Subsequently, a transcription-mediated amplification assay targeting ribosomes was developed an

64 he identification of chromosomal copy-number amplification at 1q21.3 that is enriched in subpopulatio

65 hannels that suppresses NMDAR-dependent EPSP amplification at ventral SC synapses.

66 rogenases (in approximately 60% of iCCAs) or amplifications at 11q13 and 6p21 (in approximately 15% o

67 tandard typing procedures use serological or amplification-based assays performed sequentially.

68 necessary to expand the use of nucleic acid amplification-based detection assays towards point-of-ca

69 For amplification-based resequencing of regions that cannot

70 Amplification bias is reduced considerably by degenerate

71 nd detect cross-sample contamination and PCR amplification bias.

72 f PCR cycles did not have a strong effect on amplification bias.

73 ed interspecifically and were susceptible to amplification bias.

74 ifiers (UMIs) to all samples, to account for amplification bias.

75 ircuits may have evolved not only for signal amplification but also for robustly generating bimodalit

76 p2(LacZ/LacZ) mice displayed intact cochlear amplification but impaired auditory brainstem responses.

77 Here, we test the consequence of centrosome amplification by creating mice in which centrosome numbe

78 election criteria were (1) monosomy 3 and 8q amplification by cytogenetic or DecisionDx-UM Class 2 an

79 Signal amplification by reversible exchange (SABRE) is an inexp

80 hyperpolarization of heteronuclei via signal amplification by reversible exchange (SABRE) was investi

81 animals by either Western blotting or after amplification by serial PMCAb.

82 be prohemostatic before further coagulation amplification by thrombin-dependent feedback loops enhan

83 Thus, gene amplification can be acquired and expanded through paral

84 We show that LPHN-CHDC biochip with signal amplification capability could selectively and sensitive

85 CYP19A1 amplification caused increased aromatase activity and es

86 resonance, with more than 1000 times signal amplification compared to the direct NMR detection.

87 Oncogene amplification confers a growth advantage to tumor cells

88 However, in vitro recombination during PCR amplification could not be excluded.

89 trial with a novel endovascular baroreceptor amplification device, MobiusHD (Vascular Dynamics, Mount

90 Even a complete exclusion of locus specific amplification did not exclude bias.

91 Here, we provide evidence that the 1q21 amplification-driven overexpression of ILF2 in MM promot

92 se in regulating SCR and SCR associated gene amplification/duplications and imply that these function

93 overexpression occurs in the absence of gene amplification, e.g., T cell lymphoma (TCL).

94 n of microRNA (miRNA) using electrocatalytic amplification (ECA).

95 Because it does not rely on amplification efficiency, which can be affected by seque

96 The process integrates entire oligo pool amplification, error-removal, and assembly of long DNA m

97 and it remains unclear why such independent amplification events, and associated receptor overexpres

98 Tumors arising from centrosome amplification exhibit frequent mitotic errors and posses

99 we observe that patients with 11q22.1-q22.2 amplification fail to respond to radiotherapy.

100 opy-number variation (CNV) detection and low amplification fidelity.

101 h the equilibrium and enhance the assay fold amplification for improved sensitivity and reaction spee

102 sm, a complex rearrangement followed by gene amplification, for the simultaneous formation of anomalo

103 rentiation capability by this non-enzymatic, amplification-free ADNA method.

104 Amplification-free library preparation provides single m

105 that included both sample processing and PCR amplification functions was loaded with all reagents nee

106 The successful DNA amplification has been verified off-chip by using a stan

107 Regions of tidal decrease and/or amplification highlight the non-linear response to sea-l

108 ced conversion (RT-QuIC) and tyramide signal amplification immunohistochemistry (TSA-IHC).

109 the DNA damage response for efficient genome amplification in both well-differentiated (nondividing)

110 erstanding why Gata6 is a frequent target of amplification in cancers.

111 Detection of amplification in cell-free DNA (cfDNA) from blood is str

112 report that mutant Pik3ca induces centrosome amplification in cultured cells (through a pathway invol

113 also overexpressed and associated with gene amplification in distinct CCRCC samples.

114 samples, Cys797Ser in two (17%) of 12, cMET amplification in five (50%) of ten, BRAF mutation in one

115 We demonstrate that the degree of amplification in inter-annual summer LSWT is variable, a

116 es mapped the emergence of extra-chromosomal amplification in parallel evolutionary trajectories that

117 protein expression is observed alongside MYC amplification in some cancers, in most cases protein ove

118 modeled the selection and propagation of an amplification in the BRAF oncogene (BRAF(amp)) in patien

119 PC Cx26 expression is essential for cochlear amplification in the stiff basal turn, possibly through

120 r somatic copy-number alterations, including amplifications in CDK4, FOXA1, and BCL11A.

121 n into genic regions is accompanied by local amplification, increased expression in some cases, inter

122 We show that wild-type RAS amplification increases receptor tyrosine kinase-depende

123 grade tracing combined with single-cell mRNA amplification indicated that 5-HT2B receptors are expres

124 Kelch13 mutations and for Pfplasmepsin2 gene amplification (indicating piperaquine resistance).

125 Centrosome amplification is a common feature of human tumors.

126 The level of amplification is greater in the base than in the apex of

127 rents in outer hair cells and hence cochlear amplification is greatly reduced in CD-1Cx30(A88V/A88V)

128 However, the long-term evolution of Arctic amplification is poorly constrained due to lack of conti

129 Amplification is present in approximately 10-30% of prim

130 r, it remains unclear whether this recurrent amplification is solely a manifestation of increased fit

131 Loop-mediated isothermal amplification (LAMP) is a DNA amplification approach cha

132 tly amplified using loop-mediated isothermal amplification (LAMP) method and subsequently added to lu

133 luding polymerase chain reaction, isothermal amplification, ligation, and hybridization reactions.

134 leased by dying cells, setting up a positive amplification loop between extensive cell death and unab

135 atalyzes the first enzymatic reaction of the amplification loop of the alternative pathway.

136 DAT protected the host by priming the amplification loop of type I IFN signaling.

137 toamplifies via the alternative pathway (AP) amplification loop.

138 se activity, thereby constituting a feedback amplification loop.

139 xo III digestion; thus, subsequent catalytic amplification magnifies only the intended SNV targets.

140 r for analysis without RNA extraction and/or amplification, making the sensor potentially useful for

141 Subsequently, gene amplification may have resulted via several other mechan

142 n mechanisms, or if a genomic locus-specific amplification mechanism plays a role.

143 11 functions with DDK and Mcm4 in a positive amplification mechanism to trigger the assembly of the r

144 n of increased fitness resulting from random amplification mechanisms, or if a genomic locus-specific

145 Although amplification methods facilitate single-cell genomics an

146 Isothermal amplification methods for detection of DNA and RNA targe

147 In the absence of amplification methods for proteins, the immune-detection

148 recent advances in integration of isothermal amplification methods in microfluidic devices.

149 R and greatly simplify the implementation of amplification methods in point-of-care diagnostic device

150 to most other reported isothermal norovirus amplification methods.

151 Multiplex ligation-dependent probe amplification (MLPA) did not reveal any genomic rearrang

152 When combined with post-amplification modification, the latter allows for the ex

153 r, compared with neuroblastomas without MYCN amplification, MYCN-amplified neuroblastomas expressed h

154 hat ubiquitination plays in promoting signal amplification, novel protein interactions, and protein t

155 The level of signal amplification obtained from templated reactions is a fun

156 Maximal amplification occurred when onset of vM1 optogenetic act

157 e oropharynx, and the initial phase of prion amplification occurs in the oropharyngeal lymphoid tissu

158 Amplification of 1q21 occurs in approximately 30% of de

159 various RNA-modifying enzymes prior to rapid amplification of 5' cDNA ends (5' RACE) for HIV-1 RNA an

160 Coturnix coturnix were analyzed through the amplification of a 148 bp fragment from the cyt b gene w

161 s was performed by polymerase chain reaction amplification of a fragment of tpi and bg genes.

162 As a proof of concept, amplification of a gene specific to a vancomycin-resista

163 tigate the quantum control of the weak value amplification of a qubit system coupled to a meter.

164 on, and developed degenerate primers for the amplification of arsM genes to identify methylating orga

165 xtremely high gain also leads to significant amplification of backscattered radiation from "noise", a

166 ponses without an accuracy trade-off, and an amplification of both task-related activations in dorsal

167 nfirmed by Poly(A) Polymerase-Mediated Rapid Amplification of cDNA Ends (PPM-RACE).

168 ion sites, and splicing patterns using rapid amplification of cDNA ends (RACE) and full-length cDNA s

169 Using 3' rapid amplification of cDNA ends (RACE), we mapped the 3' end

170 thways for the emergence, self-selection and amplification of chain-length- and possibly sequence-spe

171 Transmission and amplification of chemical signals across lipid bilayer m

172 al and synthetic molecules that leads to the amplification of discrete species.

173 Here we report the amplification of discrete target fragments of several ki

174 A-X, a method based on multiple displacement amplification of DNA that utilizes a thermostable mutant

175 Common approaches require extensive in vitro amplification of genomes of individual cells using DNA p

176 Deletion or amplification of IDH1 was followed by clonal expansion a

177 A is copied to support virus persistence and amplification of infection.

178 ion of the O. sinensis genome due to a rapid amplification of long terminal repeat retrotransposons t

179 Amplification of MDH1 occurs at an impressive frequency

180 cription polymerase chain reaction (qRT-PCR) amplification of miRNA extracted directly from 500 micro

181 In the DNA-based method, PCR amplification of mitochondrial D loop gene using species

182 Amplification of molecular motions into the macroscopic

183 ically without reactivation of human MYCN or amplification of murine Mycn.

184 Amplification of neural differences did not occur when c

185 e cancer mutational data, we found prevalent amplification of neuropeptide and receptors in about 72%

186 ation, the latter allows for the exponential amplification of polymers that may be functionalized wit

187 ifferent TLRs, leading to cell type-specific amplification of proinflammatory cytokines, such as TNF-

188 etal species followed by an electrocatalytic amplification of proton reduction on an inert carbon fib

189 ation effects in both studies was a specific amplification of reward magnitude in a Q-learning algori

190 ess is known about the mechanisms underlying amplification of ROS and consequent skin injury by radia

191 We have shown that amplification of sex-determining region Y-box 2 (SOX2) i

192 the parameters that determine the endogenous amplification of shocks.

193 aditional STR analyses were based on the PCR amplification of STR loci followed by gel electrophoresi

194 surface; (2) low-input requirement by linear amplification of tagged DSBs by in vitro transcription;

195 response, indicating the role of B cells in amplification of TH2 responses.

196 In a previous study we have shown that the amplification of the 11q22.1-q22.2 region, encoding cIAP

197 m Atf4 (-/-) or Atf4 (+/-) mice displayed an amplification of the amino acid response and ER stress r

198 regional climate reorganization involving an amplification of the AMO that coincided with onset of th

199 nes emerged and several were found to harbor amplification of the CDK6 kinase.

200 imate reconstructions reveal a strong winter amplification of the cooling over central and northern c

201 proach to prevent inhibitor formation before amplification of the FVIII immune response occurs.

202 t, activation of PI3KKs is a requirement for amplification of the HBoV1 genome, and HBoV1 replicates

203 y, our laboratory relied on conventional PCR amplification of the internal transcribed spacer 2 (ITS2

204 In BRCA-mutant/sporadic TNBC patients, amplification of the MYC gene is correlated with increas

205 For the childhood cancer neuroblastoma, amplification of the oncogene MYCN is associated with hi

206 Subsequent amplification of the targeted regions results in enrichm

207 Non-linear amplification of the travelling wave functioned over a b

208 Non-linear gain, or amplification of the vibrations in response to low-inten

209 duction of TSLP may be a key trigger for the amplification of type 2 inflammation in diseases.

210 omologous end joining (NHEJ) repair, enhance amplification of viral DNA.

211 omologous end joining (NHEJ) repair restrict amplification of viral DNA.

212 Genetic amplification of wild-type RAS was a recurrent mechanism

213 Concurrent amplifications of EGFR and PDGFRA have been reported in

214 ed with longer EFS after adjustment for MYCN amplification or diploidy but had no significant effect

215 r both groups with little to no benefit from amplification or from the use of DNR.

216 wild-type neuroblastoma cells harboring ALK amplification or mutations in vitro, and resulted in com

217 f the MET pathway, for example, through gene amplification or mutations.

218 may be especially effective in SCLC with MYC amplification or MYC protein overexpression.

219 rogen and progesterone receptors (ER/PR), or amplification or overexpression of HER2.

220 PAR signaling, either due to PPAR gamma gene amplification or RXRA hot-spot mutation (S427F/Y) drives

221 extremely fast because no complex labelling, amplification or wash steps are needed.

222 sequential 'daughter' nanoparticle, which by amplification over various length scales creates oriente

223 ber variants detected 5.2 deletions and 10.3 amplifications per individual with an increased fraction

224 i RLAT/KE/1957/SKINK-7 showed extensive gene amplifications, pervasive aneuploidy, and fission of chr

225 , we optimized the protein misfolding cyclic amplification (PMCA) assay for highly efficient detectio

226 on in vitro assay, protein misfolding cyclic amplification (PMCA), by using experimental scrapie and

227 The isothermal double-amplification procedure by C2CA combined with the electr

228 Here, we combined optimized prion amplification procedures with a statistical method that

229 novel biotin-labeled polymer-mediated signal amplification process combined with targeting rRNA to ex

230 These findings support the concept that an amplification process occurs along the pain neuroaxis in

231 These findings identify a unique amplification process of TNF synthesis and secretion via

232 The exceptional sensitivity stems from an amplification process triggered by incident light.

233 existence of undirected graphs with stronger amplification properties has remained open for over a de

234 ing quantitative PCR and the telomere repeat amplification protocol from PBMCs and enriched lymphocyt

235 amounts of time relative to traditional DNA amplification protocols (e.g., PCR) with an improved lim

236 (UME) (5 mum radius) using electrocatalytic amplification provided by 15 mM hydrazine in 5 mM phosph

237 TWIST1 overexpression and DNM3OS amplification provides an explanation for increased DNM3

238 unsuspected cytoplasmic events of centriole amplification, providing new perspectives for the unders

239 ailable wavenumbers q, we extract the linear amplification rate R(q) governing surface stability over

240 Isothermal rolling circle amplification (RCA) is used to detect nucleic and non-nu

241 The key signal amplification reaction for the checkpoint is the conform

242 However, the integration of a DNA amplification reaction with its associated detection in

243 To achieve the detection of the DNA amplification reaction, a real-time monitoring of the hy

244 jected to a multimethod HIVDR analysis using amplification-refractory mutation system (ARMS)-PCR, San

245 In contrast to existing methods, this amplification requires only the single-stranded DNA-bind

246 Likely, desert amplification results from the strongest water vapor fee

247 Recombinase polymerase amplification (RPA) is a novel isothermal method which r

248 y tumours with 9qA1 (Yap1) and/or 6qA2 (Met) amplification(s).

249 he design of chemical logic gates and signal amplification schemes.

250 developing single-cell multiple displacement amplification (SCMDA) and a general-purpose single-cell-

251 o anti-GD2 antibody therapy, and tumour MYCN amplification status.

252 ol in 10muL sample) for miRNA-21 without any amplification step, a complete discrimination against si

253 er-positive and -negative cells, without any amplification step, in less than 2h.

254 ecific endonuclease dsDNase for an enzymatic amplification step.

255 esonance (SPR) in combination with different amplification strategies is one of the analytical approa

256 Use of a dual hot-spot signal-amplification strategy based on AuNP-AgNP heterodimers c

257 Based on a novel signal amplification strategy the carbon dots (CDs) functionali

258 Thus, we argue that call rate (amplification success) can be used as a proxy for genoty

259 the existence within different tissues of an amplification system needed for local type 2 responses.

260 Therefore, four olive-specific amplification systems based on the trnL gene were design

261 Several prion amplification systems have been proposed for detection o

262 Typical enzyme-free amplification systems that produce a visible readout suf

263 of assays, including isothermal nucleic acid amplification techniques, enzyme-based immunoassays, and

264 antigen detection and multiplex nucleic acid amplification techniques, is becoming more widespread.

265 x by an alternative FDA-cleared nucleic acid amplification test (NAAT) but were negative for stx1 and

266 o-answer, on-demand, multiplex, nucleic acid amplification test for syndromic diagnosis of infectious

267 ort the development of a mobile nucleic acid amplification testing (mobiNAAT) platform utilizing a mo

268 Nucleic acid amplification testing (NAAT) is the preferred method to

269 However, nucleic acid amplification testing (NAAT) with the Xpert MTB/RIF assa

270 ction in certain populations by nucleic acid amplification testing (NAAT), as they involve a less inv

271 ion of diagnostic M. genitalium nucleic acid amplification testing including antimicrobial resistance

272 Nucleic acid amplification testing is a very powerful method to perfo

273 immunoassays (DIAs), and rapid nucleic acid amplification tests (NAATs) in children and adults with

274 ologic Inc., San Diego, CA) are nucleic acid amplification tests (NAATs) that detect Chlamydia tracho

275 The specificity of nucleic acid amplification tests (NAATs) used for early infant diagno

276 ly using enzyme immunoassay and nucleic acid amplification tests on stored specimens.

277 Nucleic acid amplification tests such as PCR have significantly accel

278 een designed with polynomial and exponential amplification that exhibit the modularity of linear subs

279 climatic changes often attributed to "Arctic amplification" - that is, amplified warming in Arctic re

280 We have used targeted locus amplification (TLA) to efficiently map the transgene loc

281 mal reaction extends the scope of isothermal amplification to enable more useful applications of thes

282 collateral effect of Cas13a with isothermal amplification to establish a CRISPR-based diagnostic (CR

283 ical benefit was observed in those with ESR1 amplification (tumor; 80 mg/day) and ESR1 mutation (cfDN

284 of HPV genomes in undifferentiated cells and amplification upon differentiation.

285 lly demonstrate high-gain optical parametric amplification using USRN, which is compositionally tailo

286 ive at increasing the reaction speed for RNA amplification via RT-LAMP.

287 MAF amplification was assessed by fluorescence in-situ hybri

288 ning of influenza A (H7N9) virus without DNA amplification was performed based on single-particle dua

289 ript analysis with polymerase chain reaction amplification were performed in a subset of patients.

290 e way to overcome this is to do whole-genome amplification (WGA) in clinical samples, but only limite

291 However, current whole-genome amplification (WGA) methods are limited by low accuracy

292 principle and guide finger design, revealing amplification when the cavity matches the feature size.

293 method employed is based on electrocatalytic amplification, where small quantities of metal deposit p

294 -PCR detection methods rely on linear signal amplification, which hinders the detection of small amou

295 le frequency that correlated with centrosome amplification, while loss of Hsp72 or Nek6 function in n

296 ted that blockade of Na/K-ATPase-induced ROS amplification with a specific peptide, pNaKtide, might a

297 DNA, limiting or removing the need for prior amplification with its associated biases.

298 e find positive correlation of 11q22.1-q22.2 amplification with lymph node metastasis, reduced surviv

299 its manufacturer's instructions followed by amplifications with ISSR and SCoT and agarose gel electr

300 Mathematical modelling predicted that ecDNA amplification would increase oncogene copy number and in