ライフサイエンスコーパス: anillin

1 eopontin, PRX1, LMCD1, GPR91, leiomodin, and anillin.

2 a gene involved in cellularization, encodes Anillin.

3 on of APC/C(Cdh1) increased the half-life of anillin.

4 reconstituted by adding an adaptor protein, anillin.

5 phanous and in furrow-progression mutants of Anillin.

6 emoval and retention of the scaffold protein Anillin.

7 uler for the recruitment and localization of anillin, a contractile protein and a crucial regulator o

8 We have characterized a human homologue of anillin, a Drosophila actin binding protein.

9 Anillin accumulation at the cleavage furrow is Rho guani

10 recombinant fragments, we determine that the anillin ActBD harbors three distinct actin-binding sites

11 d cell biological data suggest that multiple anillin-actin interaction modes promote the faithful pro

12 nd simultaneous knockdown of supervillin and anillin additively increases cell division failure.

13 s in a series of maternal effect and zygotic anillin alleles.

14 intermediate levels of the scaffold protein anillin allowed maximal contraction speed.

15 Anillin also localizes to ectopic cleavage furrows gener

16 Anillin, an actin-binding protein localized at the cleav

17 to the overall F-actin bundling activity of anillin and enables anillin to switch between two actin-

18 Endogenous anillin and Hcdc10 colocalize to punctate foci associate

19 LET-99 acts in a pathway parallel to anillin and is required for myosin enrichment into the c

20 We show that despite their interaction, anillin and myosin II are independently targeted to the

21 at MgcRacGAP is required for the assembly of anillin and myosin into the contractile ring.

22 ity and limit localization of both canonical anillin and non-muscle myosin II (NMM-II) to intercellul

23 These results also suggest that anillin and peanut localization are independent of actin

24 for Diaphanous in recruitment of myosin II, anillin and Peanut to the cortical region between actin

25 Two cortical proteins-anillin and RhoA-localized to presumptive abscission sit

26 structures and functional analysis of human anillin and S. pombe Mid1.

27 Cytoskeletal proteins anillin and septin have been found to be responsible for

28 R proteins are required for the retention of anillin and septin in the anterior pole, which are cytok

29 ew, including the myosin II-binding proteins anillin and supervillin, act earlier.

30 Asymmetric ingression requires Anillin and the septins, which promote the coalescence o

31 Anillins and Mid1 are scaffold proteins that play key ro

32 is maintained at the intercellular bridge by anillin, and CYK-4 is localized independently of ZEN-4 b

33 t early and late furrow proteins (Pavarotti, Anillin, and Myosin) are localized to the neuroblast bas

34 ization of F-actin as controlled by Cofilin, Anillin, and Septin.

35 partially rescued by depleting the canonical Anillin ANI-1 or blocking cytoplasmic streaming.

36 In the absence of both PIG-1/MELK and the anillin ANI-1, myosin accumulates at the anterior cortex

37 rt by limiting the accumulation of canonical anillin ANI-1.

38 rs promote accumulation of the noncannonical anillin ANI-2 at the stable cytoplasmic bridge, which in

39 ate that the cytoskeletal organizing protein anillin (ANI-1) promotes the formation of an aster-direc

40 f three C. elegans proteins with homology to anillin (ANI-1, ANI-2 and ANI-3).

41 , we identified a missense mutation R431C in anillin (ANLN), an F-actin binding cell cycle gene, as a

42 Anillins are conserved proteins that are important for s

43 an in vitro expression screen, we identified anillin as a substrate of the anaphase-promoting complex

44 to recruit actin regulators and the scaffold anillin as well as to regulate RhoA and Rac via its intr

45 Both myosin and anillin assemble into dynamic rho-dependent cortical pat

46 lly bind to RhoA and phospholipids to anchor anillin at the cleavage furrow.

47 -derived Ran-GTP signals that locally reduce Anillin at the growing cell cortex.

48 al spindle is also disrupted, revealing that anillin can also act at an early stage of cytokinesis.

49 acid changes in the C-terminal PH domain of Anillin cause defects in septin recruitment to the furro

50 video microscopy, we show that three genes: anillin, citron-kinase (CG10522), and soluble N-ethylmal

51 Here, we show that human anillin contains a conserved C-terminal domain that is e

52 Like other metazoan homologs, Drosophila anillin contains a conserved domain that can bind and bu

53 Anillin contains a conserved nuclear localization signal

54 The combined data show anillin contains a cryptic C2 domain and a Rho-binding d

55 Anillin contains a RhoA-GTP binding domain, which autoin

56 Although anillin contains conserved N-terminal actin and myosin b

57 LKB1 impinges on myosin via two pathways, an anillin-dependent pathway that also responds to the cull

58 at midcell is achieved through positive Mid1/anillin-dependent signaling emanating from the central n

59 e absence of anillin, furrows cannot form in anillin-depleted cells in which the central spindle is a

60 regulate late steps of maturation including anillin dispersal, ESCRT-III recruitment, and the format

61 The mutant anillin displays reduced binding to the slit diaphragm-a

62 nut, actin, and the actin-associated protein Anillin, do not become correctly localized in pav mutant

63 The nuclear sequestration of anillin during interphase serves to restrict anillin's f

64 d1 and show that duplication of an ancestral anillin early in the Schizosaccharomyces lineage may hav

65 Human cells depleted for anillin fail to properly regulate contraction by myosin

66 esults highlight specialization of divergent anillin family proteins in the C. elegans life cycle and

67 The short Anillin family scaffold protein ANI-2 is enriched at int

68 We found that the levels of anillin fluctuate in the cell cycle, peaking in mitosis

69 SEPT4/SEPT7/SEPT8) and the PI(4,5)P2-adaptor anillin form previously unrecognized filaments that exte

70 f the division plane positioning factor Mid1/anillin from the nucleus and negatively by the Pom1/DYRK

71 actin and the septins, likely contribute to anillin function.

72 his is a conserved mechanism for controlling anillin function.

73 We concluded that anillin functions to maintain the localization of active

74 rm and initiate ingression in the absence of anillin, furrows cannot form in anillin-depleted cells i

75 Anillin has an evolutionarily conserved capacity to asso

76 Anillins have been implicated in cytokinesis in several

77 We demonstrate that the anillin homologue Mid1, which dictates correct placement

78 tify and characterize a second fission yeast anillin homologue, Mid2p, which is not orthologous with

79 Retention of the C-terminal NLS in anillin homologues suggests that this is a conserved mec

80 Depletion of anillin in Drosophila or human cultured cells results in

81 We demonstrate upregulation of anillin in podocytes in kidney biopsy specimens from ind

82 These results reveal a novel role for Anillin in regulating epithelial cell-cell junctions.

83 ely, our data point to an important role for Anillin in scaffolding cleavage furrow components, direc

84 We propose a role for anillin in spatially regulating the contractile activity

85 hat can partly explain the essential role of Anillin in this process.

86 mislocalization of the other, and endogenous anillin increases upon supervillin knockdown.

87 hat also responds to the cullin CUL-5 and an anillin-independent pathway involving the kinase PIG-1/M

88 -dependent but may also be mediated by known anillin interactions with F-actin and myosin II, which a

89 ne/threonine kinase [8], as a putative novel anillin interactor.

90 emonstrate that the contractile ring protein anillin interacts directly with nonmuscle myosin II and

91 Anillin interacts with Rho, F-actin, and myosin II [3, 8

92 myosin II and actin do not, suggesting that anillin interacts with the septins at the cortex.

93 Anillin is a 124 kDa protein that is highly concentrated

94 These results indicate that anillin is a conserved cleavage furrow component importa

95 Anillin is a conserved component of the contractile ring

96 Anillin is a conserved protein required for cell divisio

97 Anillin is a conserved protein required for cytokinesis

98 Anillin is a conserved scaffold protein involved in orga

99 Anillin is a scaffolding protein that organizes and stab

100 n and myosin II accumulation is reduced when Anillin is depleted.

101 Collectively, these findings suggest that anillin is important in maintaining the integrity of the

102 When Anillin is knocked down, active Rho (Rho-guanosine triph

103 ns and adherens junctions are disrupted when Anillin is knocked down, leading to altered cell shape a

104 We propose that Anillin is required for proper Rho-GTP distribution at c

105 Further, anillin is required to maintain active myosin in the equ

106 Here, we show that anillin is targeted to the nucleus by importin beta2 in

107 suggest the interaction between Rho-GEFs and anillins is an important step in the signaling pathways

108 sed on the regulation of bridge stability by anillins, key regulators of cytokinetic rings and cytopl

109 In anillin knockdown cells, the cleavage furrow ingressed b

110 Additionally, we found that a shorter anillin, known to stabilize bridges [4, 7], also regulat

111 ion-and-rescue assay in Drosophila S2 cells, anillin lacking the entire actin-binding domain (ActBD)

112 which prevents CR assembly in the absence of anillin-like Mid1 and causes CRs to collapse when cytoki

113 ration of the spindle pole bodies (SPB), the anillin-like protein (Mid1p) migrates from the nucleus a

114 e [5-8]; then, massive nuclear export of the anillin-like protein Mid1 at mitosis entry confirms or r

115 control the medial cortical localization of anillin-like protein Mid1 in fission yeast.

116 Cdr1 (also known as Nim1) and Cdr2, and the anillin-like protein Mid1.

117 Under our conditions, the anillin-like protein Mid1p establishes a broad band of s

118 When a fission yeast cell divides, the anillin-like protein mid1p helps to position the contrac

119 a mutation affecting the septin-interacting, anillin-like protein Mid2, suggesting that Scw1 function

120 n important factor in this process is mid1p (anillin-like protein), which is a peripheral-membrane pr

121 sin II and F-actin do contribute, equatorial anillin localization persists in their absence.

122 divide in the middle, only S. pombe uses the anillin Mid1 as a primary nucleus-derived cue to assembl

123 two modules linked by the positional marker anillin Mid1.

124 nchorage of the contractile ring through the anillin/Mid1 family proteins from yeast to humans.

125 s containing kinase Cdr1p, kinase Cdr2p, and anillin Mid1p form in the cortex around the nucleus earl

126 (cell cycle kinases Cdr1p, Cdr2p, Wee1p, and anillin Mid1p) are constant even when the nodes disassem

127 Anillin Mid1p, Fes/CIP4 homology-Bin/amphiphysin/Rvs (F-

128 G2, mature into cytokinetic nodes by adding anillin Mid1p, myosin-II, formin Cdc12p, and other prote

129 One fission yeast homologue of anillin, Mid1p, is necessary for the proper placement of

130 Gef3 physically interacts with septins and anillin Mid2 and depends on them to localize.

131 stral microtubule-dependent pathway requires anillin, NOP-1, and LET-99.

132 itiate central spindle formation, accumulate anillin or actin at the cell equator, or undergo equator

133 pression of a septin-interacting fragment of anillin or by septin depletion via siRNA causes loss of

134 The nuf mutation does not disrupt anillin or peanut recruitment to the metaphase furrows i

135 Disassembly of anillin patches was myosin independent, suggesting that

136 d for disassembly of both myosin patches and anillin patches.

137 We conclude that supervillin and anillin play complementary roles during vertebrate cytok

138 Furthermore, we show that Anillin plays a critical role in regulating cell-cell ju

139 suggests the conserved function of Mid1-like anillin proteins may be in scaffolding, not positioning,

140 otein nanobody suggests that supervillin and anillin regulate the myosin II and actin cortical cytosk

141 ctile ring (CR) through its association with anillin-related Mid1.

142 New work shows the anillin-related protein Mid1 does not position the cytok

143 medial cortical localization and function of anillin-related protein Mid1.

144 Fission yeast cells depend on the anillin-related protein Mid1p for reliable cytokinesis.

145 Ubiquitination of anillin required a destruction-box and was mediated by C

146 ows stable filamentous structures containing anillin, Rho1, and septins to form directly at the equat

147 anillin RNAi caused gradual disruption of the intercellu

148 Mutating the NLS decreases anillin's cortical affinity, causing it to be more domin

149 anillin during interphase serves to restrict anillin's function at the cell membrane to mitosis and a

150 ugh importin beta2 binding does not regulate anillin's function in mitosis, it is required to prevent

151 ctive Ran to the equatorial membrane affects anillin's localization and causes cytokinesis phenotypes

152 Anillin's role in scaffolding the membrane cortex with t

153 laevis embryos as a model system to examine Anillin's role in the intact vertebrate epithelium, we f

154 aging-associated diminishment of the septin/anillin-scaffold causes myelin outfoldings that impair t

155 ization and/or cytokinesis, including KLP3A, Anillin, Septins, and Dynamin.

156 ate epithelium, we find that a population of Anillin surprisingly localizes to epithelial cell-cell j

157 To address the mechanism and role of anillin targeting to the nucleus in interphase, we ident

158 utant analysis between scraps, a mutation in anillin that eliminates microfilament rings, and bottlen

159 o contains a potential filament crosslinker, Anillin, that binds all three filament types.

160 Like Drosophila anillin, the human protein localizes to the nucleus duri

161 with an unusual distribution of F-actin and Anillin, these phenotypes are consistent with defective

162 ganized by the SAD-like kinase Cdr2 and Mid1/anillin through an unknown mechanism.

163 n at the cell membrane to mitosis and allows anillin to be rapidly available when the nuclear envelop

164 eptin cytoskeleton acts on the C terminus of Anillin to locally trim away excess membrane from the la

165 tron kinase Sticky acts on the N terminus of Anillin to retain it at the mature MR.

166 hoG signals through the multi-domain protein anillin to stabilize F-actin in these structures.

167 tin bundling activity of anillin and enables anillin to switch between two actin-bundling morphologie

168 Functionally, anillin was required for the completion of cytokinesis.

169 Actin, myosin II, and anillin were all concentrated in these furrows, demonstr

170 Overexpression of Cdh1 reduced the levels of anillin, whereas inactivation of APC/C(Cdh1) increased t

171 red to prevent the cytosolic accumulation of anillin, which disrupts cellular architecture during int

172 Similarly, the essential cytokinetic factor anillin, which functions at the cell membrane to promote

173 contain the COOH-terminal 197 amino acids of anillin, which includes a pleckstrin homology (PH) domai

174 wn of the essential regulator of cytokinesis anillin, which resulted in cytokinesis failure and forma

175 put promotes the simultaneous association of anillin with the plasma membrane, septins, and MTs, inde