ライフサイエンスコーパス: antibody affinity

1 Conjugation had no significant effect on antibody affinity.

2 he role of cooperativity in the evolution of antibody affinity.

3 e a particularly efficient means to increase antibody affinity.

4 elated to antigen density, target ratio, and antibody affinity.

5 tion of arginine CDR residues to the overall antibody affinity.

6 plasma cell output, T cell interaction, and antibody affinity.

7 the target structure as evidenced by higher antibody affinity.

8 e in germinal centers occurs on the basis of antibody affinity.

9 rties due to the predilection of the antigen-antibody affinity.

10 type of hot spot is preferred for increasing antibody affinity.

11 oup of B cell clones bearing a wide range of antibody affinities.

12 These mutations lowered the antibody affinity against the targeting protein and also

13 onse to HIV, somatic mutations that increase antibody affinity also increase breadth and neutralizing

14 of this improvement and explore whether TfR antibody affinity alters the intracellular trafficking o

15 Antibody affinities and avidities were determined in dir

16 Both the antibody affinity and complex dissociation rate are impo

17 on how GC cellular and clonal dynamics shape antibody affinity and diversity during the immune respon

18 endent DNA mutator on these genes to improve antibody affinity and effector functions.

19 he constant heavy chain (CH1) domain affects antibody affinity and fine specificity, challenging the

20 Based on antibody affinity and molecular weight, we identified th

21 This approach allows for predetermination of antibody affinity and specificity prior to "immunization

22 studies depend on many variables, including antibody affinity and specificity.

23 s, in combination with other factors such as antibody affinity and surface density, contributes to th

24 document any consistent correlation between antibody affinity and the ability to inhibit tumor growt

25 r optimization of cholesterol level, primary antibody affinity, and antibody-bead linkage allowed eff

26 tion, and the effect of various mutations on antibody affinity, and enable a comparison between the b

27 ontributes to shape its epitope specificity, antibody affinity, and functional activities.

28 The changes in antibody affinity are consistent with a transition from

29 Differences in antibody affinity are reflected by differences in the bi

30 region (CDR) mutagenesis is used to increase antibody affinity but can be difficult to perform succes

31 es inhibiting anti-Gal is independent of the antibody affinity, but is dependent on the concentration

32 entations on patterned surfaces designed for antibody affinity capture.

33 Interestingly, anti-Ibe10R antibody affinity chromatography of HBMEC membrane prote

34 genase digestion to produce NC1 hexamers and antibody affinity chromatography to resolve populations

35 Antibody affinity chromatography was used to fully deple

36 AQP0 C-terminal peptides and AQP0 antibody affinity chromatography were used for affinity

37 photransferase was isolated using monoclonal antibody affinity chromatography, and an alpha2beta2gamm

38 combinant protein in E. coli and purified by antibody affinity chromatography, has hemin binding acti

39 exchange chromatography and immobilized bTf antibody affinity chromatography.

40 tral salt extraction and by ion exchange and antibody affinity chromatography.

41 e, then proceeding with an ACAT-1 monoclonal antibody affinity column and an immobilized metal affini

42 Proteins that co-purified with ABCA1 on an antibody affinity column were identified by liquid chrom

43 fied as a detergent-stable complex on a CD47 antibody affinity column.

44 as purified from COS cell membranes using an antibody affinity column.

45 purified DMP1-PG with a monoclonal anti-DMP1 antibody affinity column.

46 on system and subsequently purified by a His antibody affinity column.

47 Eluted protein from this antibody-affinity column was recognized by antibodies di

48 Measurements of individual antibody affinities coupled with kinetic analysis of equ

49 ng effector cytokines and failed to increase antibody affinity following TIV receipt.

50 is buffer and were not affected by different antibody affinities for their specific epitopes.

51 Antibody affinity for antigen is believed to govern B ly

52 Antibody affinity for antigen is increased through the a

53 A), and neuraminidase (NA) and also measured antibody affinity for antigenic domains within HA.

54 of E4+ cells had mutations known to increase antibody affinity for Ars, suggesting they were products

55 Antibody affinity for BGAL(266-280) was greater than for

56 Antibody affinity for different F-protein antigenic site

57 otentially pathogenic anti-DNA antibody that antibody affinity for dsDNA does not alone determine the

58 avidity index has no simple relationship to antibody affinity for functional Env spikes on virions.

59 on Western blot, with drastic differences in antibody affinity for these bands.

60 as investigated by performing an antigen and antibody affinity immunoassay.

61 arting with subnanomolar affinity, improving antibody affinity improved cell isolation.

62 ortant function of this system is to improve antibody affinity in an ongoing response; it is turned o

63 To examine antibody affinity in T cell dependent immune responses,

64 information to develop a strategy to improve antibody affinity in vitro using phage display technolog

65 Antibody affinity is critically important in therapeutic

66 er anti-HBV antibody production is fast, the antibody affinity is high, or the levels of pre-existent

67 For example, antibody affinity is influenced by interactions of frame

68 results presented here demonstrate that the antibody affinity is strongly affected by the presence o

69 ed a folded dimeric protein with an enhanced antibody affinity (KD=400 pM).

70 This could be due to different antibody affinities, limited specificity, or distinct ta

71 eir functions in generating antigen-specific antibody affinity maturation and B cell memory.

72 itiates both somatic hypermutation (SHM) for antibody affinity maturation and DNA breakage for antibo

73 antibodies require a deeper understanding of antibody affinity maturation and evolution of the immune

74 In addition, RIG-I activation enhanced antibody affinity maturation and plasma cell responses i

75 In vitro antibody affinity maturation has generally been achieved

76 rgo a dramatic change in phenotype to enable antibody affinity maturation in germinal centers (GCs).

77 omparable to the largest values observed for antibody affinity maturation in vivo or in vitro and ind

78 Antibody affinity maturation involves selective survival

79 n germinal centers and provide evidence that antibody affinity maturation is driven by competition fo

80 ological responses with diverse SHM-mediated antibody affinity maturation pathways and divergent epit

81 al B-cell expansions, and also for following antibody affinity maturation processes.

82 s in TIV-induced CD4(+) T-cell expansion and antibody affinity maturation suggests that LypW may pred

83 hese GCs then rapidly regressed and GC-phase antibody affinity maturation was reduced.

84 nteraction with helper T cells, and promoted antibody affinity maturation while antagonizing IRF4-dri

85 g de novo antigen recognition and subsequent antibody affinity maturation without initial preimmune B

86 Antibody affinity maturation, a hallmark of adaptive imm

87 Germinal centers (GCs) are the site of antibody affinity maturation, a process that involves co

88 f B cell tolerance seemingly occurred during antibody affinity maturation, an obligatorily T cell-dep

89 ed T cell-dependent antibody production, IgG antibody affinity maturation, and germinal center (GC) f

90 y signal for immunoglobulin class switching, antibody affinity maturation, and priming of CD8+ T-cell

91 o provide the antigenic stimulus that drives antibody affinity maturation, but their role in cellular

92 During antibody affinity maturation, germinal center (GC) B cel

93 in antibody variable regions is critical for antibody affinity maturation, with HIV-1 broadly neutral

94 nd T cells and required for GC formation and antibody affinity maturation.

95 n, suggests an overall strategy for in vitro antibody affinity maturation.

96 h declined rapidly, and induced only minimal antibody affinity maturation.

97 one cells and an inability to undergo robust antibody affinity maturation.

98 he dark zone (DZ) and light zone (LZ) during antibody affinity maturation.

99 l hotspots strategically located to optimize antibody affinity maturation.

100 (SHM) of Ig genes in B cells is crucial for antibody affinity maturation.

101 the phase of proliferative expansion and of antibody affinity maturation.

102 , become germinal centre B cells and undergo antibody affinity maturation.

103 erminal centers (GCs) are important sites of antibody affinity maturation.

104 ermline hot spots are effective for in vitro antibody affinity maturation.

105 rminal center (GC) B cells, thereby allowing antibody affinity maturation.

106 ts of the humoral immune response, including antibody affinity maturation.

107 can be engineered to initiate the process of antibody-affinity maturation against one of the most con

108 However, antibody-affinity maturation in vivo often fails to prod

109 nd safe immunotherapeutic drugs, fully-human antibodies affinity-matured in a healthy host to a tumor

110 1.8- and 1.7-fold, respectively; and for an antibody affinity of 10(-8) M, increasing t(1/2-I) from

111 Even though the effect of antibody affinity on neutralization potency is well docu

112 to motor nerves is not due to differences in antibody affinity or ceramide structural specificity.

113 of silicon photonic microring resonators for antibody affinity profiling and one-step ricin detection

114 Here we report the antibody affinity purification of a Drosophila TRF2-cont

115 oth human monoclonal anti-DNA antibodies and antibodies affinity purified from the sera of patients w

116 Anti-Neu5Gc antibodies affinity-purified from human serum also direc

117 IgG-enriched fractions and anti-52-kD SSA/Ro antibodies affinity-purified from sera of mothers whose

118 However, injection of anti-Abeta antibodies, affinity-purified from immunized Tg-SwDI mic

119 e compared between intact MVs and monoclonal antibody affinity-purified MV ALP.

120 ed from cleavage of peptide substrates using antibody affinity-purified toxins are detected by MS.

121 oss-linking followed by positive or negative antibody affinity selection of individual stable conform

122 B cells are eliminated during development by antibody-affinity selection and receptor-editing mechani

123 To better understand antibody affinity/specificity trade-offs, we have select

124 Antibody affinity was measured by using a competitive pe

125 s using natural and designed TM domains, and antibody affinity was measured using immobilized and sol

126 To understand the importance of primary antibody affinity, we compared a series of point mutants

127 recognized by HyHEL-63, and their effects on antibody affinity were measured using surface plasmon re

128 ith both procedures, apparent differences in antibody affinity were observed with the new test which

129 C1, and citrullinated C1, together with the antibody affinity, were investigated in OVA-mediated art

130 ving protein-protein docking and engineering antibody affinity, which will help accelerate the develo

131 can accumulate charged residues to increase antibody affinity without compromising specificity.