ライフサイエンスコーパス: anticodon

1 ng G37 to m(1)G37 on the 3' side of the tRNA anticodon.

2 anscribed with the methionine-specifying CAU anticodon.

3 nylyate residue by recognizing the tRNA(His) anticodon.

4 to utilize the more specific tRNA(Leu(UUR)) anticodon.

5 iptional modifications in or adjacent to the anticodon.

6 common their contribution of order to tRNA's anticodon.

7 gle central-mismatch to the histidyl-tRNAQUG anticodon.

8 tRNA(Pro14) D-loop or within the tRNA(Lys43) anticodon.

9 divergent tRNA(Gly)UCA with an opal-decoding anticodon.

10 upling of specific amino acids with specific anticodons.

11 d P congruent with 2.1 x 10(-46) for cognate anticodons.

12 s or by non-native tRNAs with exact-matching anticodons.

13 ned for 31 tRNA isodecoders (all contain CUA anticodon), 21 derived from four isoacceptor families of

14 uclear tryptophanyl tRNA that contains a CCA anticodon able to decode the UGG codons used in cytoplas

15 esis, we have assessed the dispersion of the anticodon-acceptor angle for bovine mtRNA(Ser)(AGY), whi

16 ions likely results in greater dispersion of anticodon-acceptor interstem angle than for canonical tR

17 ated mRNAs, whether this might extend to the anticodon-adjacent position 37 was unknown.

18 Contrary to previous observations, the anticodon adopts the same conformation as seen in mature

19 priate combination of contributions from the anticodon, amino acid and tRNA body.

20 Ribosomal anticodon-amino acid enrichment further reveals that spe

21 fossil, preserving biological evidence that anticodon-amino acid interactions shaped the evolution o

22 ation on how a tRF is distributed across all anticodon/amino acid combinations, provides alignments b

23 us to report on their genomic distributions, anticodon/amino acid properties, alignments, etc. while

24 Aminoacyl-tRNA synthetases recognize tRNA anticodon and 3' acceptor stem bases.

25 aRSs may have prevented the conflict between anticodon and amino-acid identities of recruited tRNAs.

26 of a tRNA gene, a mutation that changes the anticodon and the loss of the ancestral tRNA gene.

27 pairing mismatches between the peptidyl-tRNA anticodon and the mRNA codon dramatically delay this rat

28 thylation of tRNA(Pro) on the 3' side of the anticodon and the translation factor EF-P.

29 n the first two nucleotides of the codon and anticodon and then is stabilized by base-stacking energy

30 EAT-like domain to recognize the appropriate anticodons and position the hypermodified nucleoside int

31 an invariant 3'-terminal CCA, trinucleotide anticodons and tRNA bodies.

32 pecifier sequence, which recognizes the tRNA anticodon, and the antiterminator bulge, which base pair

33 en the classic genetic code, embodied in the anticodon, and the second, or RNA operational, code that

34 ural codons and tRNAs with cognate unnatural anticodons, and their efficient decoding at the ribosome

35 aryotes, transfer RNAs (tRNAs) with the same anticodon are encoded by multiple nuclear genes, and lit

36 velopment, the pools of mRNA codons and tRNA anticodons are invariant and highly correlated, revealin

37 We show here that anticodons are selectively enriched near their respectiv

38 ancement of the interaction between the tRNA anticodon arm and the Specifier Loop domain.

39 odon interaction, and the length of the tRNA anticodon arm is then measured by the distal loop-loop p

40 known to be responsible for several of the D/anticodon arm modifications, but methylases catalyzing p

41 y altering the structure and dynamics of the anticodon arm of the aminoacyl-tRNA.

42 0S subunit head domain and in flexing of the anticodon arm of tRNA suggests that they represent gener

43 Significant contacts with the anticodon arm were not observed.

44 ic properties of Bacillus subtilis tRNA(Tyr) anticodon arms containing the natural base modifications

45 fer RNA occur at five positions in the D and anticodon arms, and at positions G6 and G7 in the accept

46 xtended with regions homologous to TPsiC and anticodon arms.

47 nstrate that modifications pre-structure the anticodon as a key prerequisite for efficient and accura

48 oacyl-tRNA synthetases (aaRSs) that use tRNA anticodons as identity elements can be considered allost

49 heless, His-Phe sites appear enriched in His anticodons, as previous L: -His sites also were.

50 minoacylation kinetics, assists proper codon-anticodon base pairing at the ribosome A-site, and preve

51 initiation complexes with established codon-anticodon base-pairing.

52 S include acceptor stem elements G72/A73 and anticodon bases G35/G36.

53 estricted the conformational dynamics of the anticodon bases of the modified hmtASL(Met)(CAU).

54 fferences between both types of GlyRS at the anticodon binding domain level are evident, the extent a

55 ts for CspA (cold shock protein A) and LysN (anticodon binding domain of lysyl tRNA synthetase).

56 both its N-terminal catalytic and C-terminal anticodon binding domains and that the catalytic domain

57 n of the tRNA acceptor stem is enhanced upon anticodon binding.

58 ytic domain and flexibly attached C-terminal anticodon-binding domain (CTD); and (ii) the catalytic d

59 ts A-C) and the Urzyme supplemented with the anticodon-binding domain (fragments A, C, and D).

60 e results entirely from coupling between the anticodon-binding domain and an insertion into the Rossm

61 5% of the contemporary enzyme, including the anticodon-binding domain and connecting peptide 1, CP1,

62 recruitment of connecting peptide 1 and the anticodon-binding domain during evolutionary development

63 he active site of one monomer pairs with the anticodon-binding domain from the other.

64 also propose that structural features of the anticodon-binding domain in MST1 permit binding of the e

65 ylate tRNA(1Thr), reveals differences in the anticodon-binding domain that probably allow recognition

66 es, one in the catalytic- and another in the anticodon-binding domain.

67 gth TyrRS contains a catalytic domain and an anticodon-binding domain; however, the two halves retain

68 e Urzymes acylate cognate tRNAs even without anticodon-binding domains, in keeping with the possibili

69 ned, whereas the new NLS was evolved from an anticodon-binding hexapeptide motif.

70 By reprogramming the anticodon-binding pocket of Pyrococcus horikoshii ProRS

71 ligomeric transport complex composed of nine anticodon-binding-domain-like units that give rise to a

72 tRNA isodecoders share the same anticodon but have differences in their body sequence.

73 However, mutations in the tRNA(His) anticodon caused a drastic loss of in vitro histidylatio

74 the conserved purine-37, 3'-adjacent to the anticodon, causing expression of alternate protein seque

75 and carboxylic acid side-chains, whereas the anticodon codes for a wider range of such properties, bu

76 op likely causes tRNA distortion and affects anticodon-codon interaction, which induces +1 frameshift

77 (6)A(37) participate in the stability of the anticodon-codon interaction.

78 ide interactions that disengage the ribosome anticodon-codon interactions for slippage.

79 nces translation fidelity by stabilizing the anticodon/codon interaction in the ribosomal decoding si

80 ection begins with the base pairing of codon-anticodon complex between the m-RNA and tRNAs.

81 er side (5' and 3'), we consider the risk of anticodon confusion and subsequent erroneous aminoacylat

82 yl-tRNAs (Af-tRNA(Pro)) with three different anticodons: CUA, AGGG, and CUAG.

83 tRNA anticodon damage inflicted by secreted ribotoxins such a

84 g of codon 46 with its cognate peptidyl-tRNA anticodon dissociates, and following mRNA slippage, pept

85 assettes, it is shown that the peptidyl-tRNA anticodon does not scan the intervening sequence for pot

86 ), and pseudouridine (Psi(39)) in the tRNA's anticodon domain are critical for ribosomal binding and

87 dicate strongly that modifications of tRNA's anticodon domain control gene expression.

88 djacent to the PBS, and the modified, U-rich anticodon domain of tRNA(Lys3).

89 The modification contributes to the tRNA's anticodon domain structure, thermodynamic properties and

90 ls the htRNA's amino acid accepting stem and anticodon domains in preparation for their being anneale

91 structure reveals stabilization of the codon-anticodon duplex by the N-terminal tail of eIF1A, change

92 and INS require these same acceptor stem and anticodon elements, respectively, whereas YbaK lacks inh

93 Other tRNA(Sec) species with different anticodons enabled E. coli to synthesize active formate

94 nts with two bits per base, we show that the anticodon encodes the hydrophobicity of each amino acid

95 ique sites on proto-tRNAs (distinct from the anticodons), expansion of the code via proto-tRNA duplic

96 Both cognate and near-cognate tRNA anticodons explore the aminoacyl-tRNA-binding site (A si

97 an 500 interspersed tRNA genes comprising 51 anticodon families of largely unequal copy number.

98 stem, the T-stem base pair G51:C63, and the anticodon flanking nucleotides U33 and A37.

99 ration of the structural requirements of the anticodon for aminoacylation by methionyl tRNA synthetas

100 y conserved Class I active-site residues are anticodons for corresponding Class II active-site residu

101 several misacylated tRNAs containing the GAC anticodon from the A site showed little indication for s

102 cently, protect the tRNAs with complementary anticodons from confusion in translation.

103 that of both cognate and near-cognate codon-anticodon helices.

104 RNAs by monitoring the geometry of the codon-anticodon helix in the decoding center using the univers

105 nucleotide G530 stabilizes the cognate codon-anticodon helix, initiating step-wise 'latching' of the

106 nds with the 2'-hydroxyl groups of the codon-anticodon helix, which are expected to be disrupted with

107 ed to be disrupted with a near-cognate codon-anticodon helix.

108 oacceptor family than to tRNAs with the same anticodon in related species.

109 l basis for the more significant role of the anticodon in tRNA recognition by the class II enzyme.

110 between the start codon in the mRNA and the anticodon in tRNA(i).

111 ions found at position 37, 3-adjacent to the anticodon, in tRNAs responsible for ANN codons.

112 reveals forty-five of the sixty-one possible anticodons indicating widespread use of 'wobble' tRNAs.

113 equire an RNA pseudoknot that mimics a codon-anticodon interaction and contains a conformationally dy

114 e effect on arrangement of mRNA at the codon-anticodon interaction area.

115 Visualization of the codon*anticodon interaction by X-ray crystallography revealed

116 ontext of A-form RNA and its effect on codon-anticodon interaction during ribosome binding.

117 transition from ancient amino acids without anticodon interaction to newer additions with anticodon

118 of aa-tRNA, proposed to signal cognate codon-anticodon interaction to the GTPase centre and tune the

119 is first examined through specifier sequence-anticodon interaction, and the length of the tRNA antico

120 In addition to the specifier-anticodon interaction, two interdigitated T-loops near t

121 nticodon interaction to newer additions with anticodon interaction.

122 o maintain structure and stabilize the codon-anticodon interaction.

123 anslation in eukaryotes is governed by codon-anticodon interactions between the initiator Met-tRNA(i)

124 n of eIF1, which is thought to monitor codon-anticodon interactions during translation initiation, li

125 I hypothesise that codon-anticodon interactions of tRNAs with mRNA evolved as a m

126 The effect of E-site codon:anticodon interactions on +1 PRF was also experimentally

127 bind specific tRNA anticodons through codon-anticodon interactions with the nucleotide triplet of th

128 for a complete set of single-mismatch codon-anticodon interactions.

129 oduced when a tRNA suppressor containing CUA anticodon is co-transfected with the GFP gene.

130 The three-dimensional structure of the anticodon is crucial to tRNA-mRNA specificity, and the d

131 Because the anticodon is not important for PylRS recognition, a tRNA

132 34 (U34) at the wobble position of the tRNA anticodon is post-transcriptionally modified, usually to

133 ancies by grouping Pol III occupancy into 46 anticodon isoacceptor families or 21 amino acid-based is

134 s an unusual tRNA(Thr) with an enlarged 8-nt anticodon loop ( ).

135 usual tRNA(1Thr), which contains an enlarged anticodon loop and an anticodon triplet that reassigns t

136 tides that pair with nucleotides in the tRNA anticodon loop and is flanked on one side by a kink-turn

137 cellular processes, including stress-induced anticodon loop cleavage of mature tRNAs to generate tRNA

138 NAs, which imparts a unique structure to the anticodon loop enhancing its binding to ribosomes in vit

139 thione-dependent formation of (m1)G37 in the anticodon loop for efficient aminoacylation.

140 the tRNA at the 5' end while maintaining the anticodon loop for potential loop-loop interactions.

141 Our data show that MST1 recognizes the anticodon loop in both tRNAs, but employs distinct recog

142 3 is required for deposition of m(5)C at the anticodon loop in the mitochondrially encoded transfer R

143 not result in this signature feature of the anticodon loop in tRNA(Tyr).

144 the hydrogen bond network of the unmodified anticodon loop including a C(32)-A(38)(+) base pair and

145 The size but not the sequence of the anticodon loop is critical for tRNA(1Thr) recognition, w

146 The insertion of U33.5 in the anticodon loop likely causes tRNA distortion and affects

147 wobble position of certain tRNAs, a critical anticodon loop modification linked to DNA damage surviva

148 enosine (t(6)A), found at position 37 in the anticodon loop of a subset of tRNA.

149 against a fungal ribotoxin that incises the anticodon loop of cellular tRNAs.

150 sufficient to promote the U-turn fold of the anticodon loop of Escherichia coli tRNA(Phe), but these

151 s site, NEG contacts 16S rRNA as well as the anticodon loop of the A-site tRNA.

152 e targeted nucleotide A37 flips out from the anticodon loop of tRNA and flips into a channel in DMATa

153 re shows how SmpB plays the role of both the anticodon loop of tRNA and portions of mRNA to facilitat

154 's in hairpin loops, which is similar to the anticodon loop of tRNA targeted by adenosine deaminases

155 accessibility for pairing with bases in the anticodon loop of tRNA.

156 omain in MST1 permit binding of the enlarged anticodon loop of tRNA1(Thr).

157 Here we show that cytosine 32 in the anticodon loop of Trypanosoma brucei tRNA(Thr) is methyl

158 These enzymes affect the anticodon loop of various tRNAs and can impact protein s

159 the xo (5)U 34-type modifications order the anticodon loop prior to A-site codon binding for an expa

160 n an interaction network, extending from the anticodon loop through h44 and protein S12 to the EF-Tu-

161 We identified tRNA(Opt)AUG anticodon loop variants that increase reassignment of th

162 ions can induce cleavage of tRNAs around the anticodon loop via the use of the ribonuclease angiogeni

163 We found that an extended anticodon loop with an extra nucleotide was required for

164 s through pairing of nucleotides in the tRNA anticodon loop with nucleotides in the Specifier Loop do

165 IPTase recognition sequence A36A37A38 in the anticodon loop, only tRNA(Ser)AGA, tRNA(Ser)CGA, tRNA(Se

166 far away from the minihelix domain as in the anticodon loop, prevents efficient CCA addition.

167 an unusual thiolation at position 33 of the anticodon loop, the only known modification at U33 in an

168 riminate the purposefully broken ends of the anticodon loop.

169 tRNA variants with an expanded or contracted anticodon loop.

170 recently was demonstrated to hypermodify the anticodon loops in some tRNAs.

171 aminoacylates two natural tRNAs that contain anticodon loops of different size and sequence.

172 RNAs, tRNA1(Thr) and tRNA2(Thr), that harbor anticodon loops of different size and sequence.

173 ng post-transcriptional modifications of the anticodon loops of four tRNAs in Escherichia coli.

174 ngle aaRS can recognize completely divergent anticodon loops of natural isoacceptor tRNAs and that in

175 recently showed that ANG cleaves tRNA within anticodon loops to produce 5'- and 3'-fragments known as

176 ivated ribonuclease that cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced f

177 hanism by which a single aaRS binds distinct anticodon loops with high specificity is not well unders

178 t probably allow recognition of the distinct anticodon loops.

179 ibosome selects aminoacyl-transfer RNAs with anticodons matching the messenger RNA codon present in t

180 ilization of the closed PIN state with a UUG-anticodon mismatch.

181 odons has evolved beyond a simple tripeptide anticodon model.

182 and show by direct measurement, that a tRNA anticodon modification from guanosine to queuosine has c

183 , and structural analyses indicated that the anticodon modifications enhanced order in the loop.

184 The importance of individual and combined anticodon modifications to the tRNA/HIV-1 Loop I RNA's i

185 s an abstraction of how mRNA codons and tRNA anticodons molecularly interact during protein synthesis

186 site in a manner resembling that of the tRNA anticodon-mRNA codon.

187 a regular tRNA(2Thr) with a threonine (Thr) anticodon, MST1 also recognizes an unusual tRNA(1Thr), w

188 One notable exception was the G34A anticodon mutation of hmt-tRNA(Phe) (mitochondrial DNA m

189 search in hundreds of genomes revealed that anticodon mutations occur throughout the tree of life.

190 Suppressor tRNAs bear anticodon mutations that allow them to decode premature

191 RNA damage inflicted by the Escherichia coli anticodon nuclease PrrC (EcoPrrC) underlies an antiviral

192 We demonstrate that the anticodon nucleotides of these misacylated tRNAs play a

193 ifier trinucleotide that base pairs with the anticodon of cognate tRNA.

194 report the existence of pseudouridine in the anticodon of Escherichia coli tyrosine transfer RNAs (tR

195 ader is inspected for complementarity to the anticodon of methionyl initiator transfer RNA (Met-tRNAi

196 sed on a strategic mutation that changed the anticodon of other tRNA genes to match that of the delet

197 n is the formation of base pairs between the anticodon of the aminoacyl-tRNA and the mRNA codon in th

198 Pairing of the Specifier Sequence with the anticodon of the cognate tRNA is the primary determinant

199 potential base-pairing interactions with the anticodon of the initiator methionyl tRNA.

200 ctions between the codon on the mRNA and the anticodon of the initiator tRNA.

201 Hypermodifications near the anticodon of tRNA are fundamental for the efficiency and

202 Stem I recognizes the overall shape and anticodon of tRNA, while a 3' domain evaluates its amino

203 led that the modification of cytidine in the anticodon of tRNA2(Ile) adds 112 mass units to its molec

204 In most cases, the trinucleotide anticodons of tRNA are important identity determinants f

205 RNA pool, demonstrating that mutation in the anticodons of tRNA genes is a common adaptive mechanism

206 Mutations in the tRNA anticodon or at the discriminator base had little to no

207 nal phenotypes, with enzymes known to modify anticodons, or non-tRNA substrates such as rRNA, exhibit

208 tension on the mRNA that destabilizes codon-anticodon pairing in the P site and promotes slippage of

209 This interaction mimics codon-anticodon pairing in translation but occurs in the absen

210 racting directly with the mRNA through codon/anticodon pairing.

211 t S12 is involved in the inspection of codon-anticodon pairings in the ribosomal A site, as well as i

212 We analyze the anticodon pairs complementary to the face-to-face codon

213 opment is controlled in order to generate an anticodon pool that closely corresponds to messenger RNA

214 of adenosine (A) to inosine (I) at the first anticodon position in tRNA is catalyzed by adenosine dea

215 Modification of the cytidine in the first anticodon position of the AUA decoding tRNA(Ile) (tRNA2(

216 eranylated residues are located in the first anticodon position of tRNAs specific for lysine, glutami

217 While mutations in the tRNA anticodon preferentially affected the thermodynamics of

218 red pseudohyphal growth, showing altered CUG anticodon presentation cannot itself induce pseudohyphal

219 g model based upon these data suggested that anticodon recognition by LysRS1 relies on considerably f

220 focusing attention on the mechanism of tRNA anticodon recognition by LysRS1.

221 We identified a hexapeptide motif in the anticodon recognition domain that is critical for nuclea

222 the N-terminal half) and only one functional anticodon recognition site (contributed by the C-termina

223 lity that acceptor stem recognition preceded anticodon recognition.

224 rse modifications of nucleotide bases in the anticodon region modulate this specificity.

225 Q is present in the anticodon region of tRNA(GUN) in Eukarya and Bacteria, w

226 ermal stabilities of variously modified tRNA anticodon region sequences bound to the Loop I of viral

227 ppreciably stabilized the interaction of the anticodon region with the viral subtype G and B RNAs.

228 r24)) for cleavage at, or adjacent to, their anticodons, resulting in the generation of tRNA halves.

229 Modifications at the anticodon's wobble position are required for recognition

230 ical for tRNA(1Thr) recognition, whereas the anticodon sequence is essential for aminoacylation of tR

231 odons, depending on the identity element and anticodon sequence of the tRNA.

232 e bacterial ortholog that 'reads' the entire anticodon sequence, MST1 recognizes bases in the second

233 position and the nucleotide upstream of the anticodon sequence.

234 onsible for the matching of amino acids with anticodon sequences of tRNAs.

235 amino acid to a set of tRNAs with conserved anticodon sequences.

236 rall architecture of tRNA in addition to its anticodon, something accomplished by large ribonucleopro

237 quence nucleotides, which pair with the tRNA anticodon, stack with their Watson-Crick edges rotated t

238 ed the structure and function of this tRNA's anticodon stem and loop (ASL) domain with these modifica

239 nding and functional remodeling of the human anticodon stem and loop domain (hASL(Lys3)) were studied

240 The structure of the tRNA anticodon stem and loop domain (hmtASL(Met)(CAU)), deter

241 e first synthesis and analyses of the tRNA's anticodon stem and loop domain containing the 5-formylcy

242 inities of the unmodified and fully modified anticodon stem and loop domains of tRNA (Val3) UAC (ASL

243 The anticodon stem and loop of these isoacceptors (ASL(Arg1,

244 having base changes in the acceptor stem or anticodon stem and loop still retained the ability to co

245 ceptor stem, the first two base pairs of the anticodon stem and the first nucleotide of the variable

246 that modification of the G:C content of the anticodon stem and therefore reducing the structural fle

247 C terminus is required to protect a pre-tRNA anticodon stem from chemical modification.

248 he 30S ribosomal subunit in complex with the anticodon stem loop of tRNA(Ser) bound to the PsiAG stop

249 s Our structure of QueG bound to a tRNA(Tyr) anticodon stem loop shows how this enzyme uses a HEAT-li

250 position 37 (m(1)G37) modification-deficient anticodon stem loop(Pro), both of which cause the riboso

251 s occupy the 50S subunit E site, while their anticodon stem loops move with the head of the 30S subun

252 d with respect to the large subunit, and the anticodon stem loops reside in the A and P sites of the

253 ree consecutive G-C base (3G-C) pairs in the anticodon stem of initiator tRNA.

254 IN state) and by their interactions with the anticodon stem of Met-tRNAi.

255 ed, defining positions in the tRNA(Gln)(CUG) anticodon stem that restrict first base wobble.

256 t conserved bases throughout tRNAi, from the anticodon stem to acceptor stem, play key roles in ensur

257 uting the conserved G31:C39 base pair in the anticodon stem with different pairs reduces accuracy (th

258 erved three consecutive GC base pairs in its anticodon stem, play a crucial role in ribosome maturati

259 The anticodon stem-loop (ASL) of transfer RNAs (tRNAs) drive

260 ridine (Psi) formation at position 28 in the anticodon stem-loop (ASL).

261 hat streptomycin stabilizes the near-cognate anticodon stem-loop analogue complex, while destabilizin

262 gue complex, while destabilizing the cognate anticodon stem-loop analogue complex.

263 d yet improves recognition of a near-cognate anticodon stem-loop analogue.

264 it in complexes with cognate or near-cognate anticodon stem-loop analogues and messenger RNA.

265 ptomycin disrupts the recognition of cognate anticodon stem-loop analogues and yet improves recogniti

266 Through randomizing bases in anticodon stem-loop followed by a functional selection,

267 The anticodon stem-loop of the A-site tRNA is captured in tr

268 boxyl-terminal domain positioned next to the anticodon stem-loop of the P site-bound initiator tRNA.

269 The anticodon stem-loop of tRNAs requires extensive posttran

270 resolution) or two (4.0 angstrom resolution) anticodon stem-loop tRNA mimics bound, that reveal inter

271 it strategy that completely unfolds the tRNA anticodon stem-loop, which is likely critical for recogn

272 with a kink and twist between the D-stem and anticodon stem.

273 .4-A resolution) of MjPSTK complexed with an anticodon-stem/loop truncated tRNA(Sec) (Mj*tRNA(Sec)),

274 sting formation of correctly folded pre-tRNA anticodon stems in vivo.

275 and a widened angle between the acceptor and anticodon stems.

276 secutive G-C base pairs (3GC pairs) in their anticodon stems.

277 Located adjacent and 3' to the anticodon, t(6)A(37) is a conserved modification that is

278 nd differentiation-induced tRNAs often carry anticodons that correspond to the codons enriched among

279 elements in C. crescentus tRNA(His) are the anticodon, the discriminator base and U72, which are rec

280 ity riboswitches that can bind specific tRNA anticodons through codon-anticodon interactions with the

281 stacking while ms(2)t(6)A(37) restrained the anticodon to adopt an open loop conformation that is req

282 vidence that modifications help preorder the anticodon to allow it to recognize the codons, however,

283 quence of the glyQS leader RNA and tRNA(Gly) anticodon to test the effect of all possible pairing com

284 brucei by specifically editing the tRNA(Trp) anticodon to UCA, which can now decode the predominant m

285 t1) modify tRNA position 37, adjacent to the anticodon, to N6-isopentenyladenosine (i6A37) in all cel

286 We reasoned that anticodon-triggered conformational change might be restr

287 pid coding systems matching amino acids with anticodon trinucleotides.

288 h contains an enlarged anticodon loop and an anticodon triplet that reassigns the CUN codons from leu

289 ed orthogonal M. jannaschii tRNA with an AUG anticodon: tRNA(Opt) We suspected a modification of the

290 base at the 3' position adjacent to the tRNA anticodon, using S-adenosyl methionine (AdoMet) as the m

291 hProRSs show specificity toward a particular anticodon variant of Af-tRNA(Pro), whereas others are pr

292 suspected a modification of the tRNA(Opt)AUG anticodon was responsible for the anomalous lack of codo

293 ity (approximately 21%) of modern codons and anticodons were assigned via RNA binding sites.

294 he A-rich HIV Loop I sequence and the U-rich anticodon, whereas the tRNA's Psi(39) stabilized the adj

295 served nucleotide(A37), adjacent (3') to the anticodon, which is important for the fidelity of codon

296 e substitution at the middle position of the anticodon, which resulted in the change of not only the

297 nally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative c

298 of 5-taurinomethyluridine (taum(5)U) in the anticodon wobble position of five mitochondrial tRNAs.

299 osine (Q) is almost universally found in the anticodon wobble position of specific tRNAs.

300 ncm(5) modifications to uridine in the tRNA anticodon 'wobble' position in both yeast and higher euk