ライフサイエンスコーパス: antigenomic

1 ccurring assignments in the genomic (4G) and antigenomic (4U) promoters are optimal for transcription

2 used were such that the RNA transcripts were antigenomic and began at the same 5' site as the mRNA pr

3 ree of functional similarity between the RSV antigenomic and genomic promoters, but provided a furthe

4 uroblastoma cells contained both genomic and antigenomic BDV RNA species as well as an enrichment of

5 ubstitutions were recovered from full-length antigenomic cDNA and analyzed for their ts, att, and ca

6 stitutions was introduced into an infectious antigenomic cDNA clone derived from hRSV A2 strain.

7 ntroduced individually into the RSV A2 (rA2) antigenomic cDNA, and recombinant viruses, rA2-P172 and

8 The NS2 knockout antigenomic cDNAs were cotransfected with plasmids encod

9 y sequenced for each of the nonpolyhexameric antigenomic cDNAs.

10 specifically directed against the genomic or antigenomic HCV sequences.

11 that the J(-1/1) stretch is conserved among antigenomic HDV isolates.

12 mational change accompanies catalysis in the antigenomic HDV ribozyme in solution, similar to the cat

13 tween the precursor and product forms of the antigenomic HDV ribozyme, consistent with differences in

14 roduct solution structures of the cis-acting antigenomic HDV ribozyme.

15 there was a proportionally large fraction of antigenomic HDV RNA in the cytoplasm at early time point

16 d liver there were additional polyadenylated antigenomic HDV RNA species with 5' ends located at leas

17 Thus, these results suggest that genomic and antigenomic HDV RNA syntheses are performed by two diffe

18 abilized cells, the synthesis of full-length antigenomic HDV RNA was completely resistant to high con

19 y in both the nucleus and cytoplasm, whereas antigenomic HDV RNA was mostly retained in the nucleus,

20 In contrast, while antigenomic HDV RNA was predominantly in the nucleus, th

21 tiple of six, we constructed six full-length antigenomic HPIV2/V94 cDNAs that deviated from a polyhex

22 ed by transfecting plasmids that contain the antigenomic L and S RNA segments of PICV under the contr

23 For example, both genomic and antigenomic linear RNAs were acceptable.

24 ermination, we constructed plasmids encoding antigenomic minireplicons containing one or two transcri

25 s and be transcribed, generating genomic and antigenomic multimeric forms.

26 ernatively, was a consequence of genomic and antigenomic NTRs having similar functions requiring sequ

27 therefore established the potential value of antigenomic PNA therapy for patients with heteroplasmic

28 ff assay under physiological conditions, the antigenomic PNAs specifically inhibited replication of m

29 latively smaller amounts of an 800-nt RNA of antigenomic polarity that is polyadenylated and consider

30 Viral RNA of antigenomic polarity, whether expressed from genomic RNA

31 I and CRII, optimal replication from the SV5 antigenomic promoter requires a third sequence-dependent

32 Thus, optimal RNA replication from the SV5 antigenomic promoter requires three sequence-dependent e

33 sequences in place of bases 21 to 72 of the antigenomic promoter, and the relative level of RNA repl

34 t signal transcription in the context of the antigenomic promoter.

35 ences are thought to contain the genomic and antigenomic promoters.

36 However, we find that the antigenomic ribozyme is 100 to 1000-fold less active und

37 An HDV antigenomic ribozyme precursor RNA that included the ups

38 Here we used a trans-acting form of the antigenomic ribozyme to examine the contribution of the

39 creased substantially, while activity of the antigenomic ribozyme was enhanced by introducing a CAA s

40 s 35S promoter and the hepatitis delta virus antigenomic ribozyme with a downstream nopaline synthase

41 In the hepatitis delta virus antigenomic ribozyme, imidazole buffer rescued activity

42 an in Ca2+ while the reverse is true for the antigenomic ribozyme.

43 the role of cytosine in more detail with the antigenomic ribozyme.

44 Self-cleavage of the genomic and antigenomic ribozymes from hepatitis delta virus (HDV) r

45 Hepatitis delta virus (HDV) uses genomic and antigenomic ribozymes in its replication cycle.

46 leavage by closely related 84 nt genomic and antigenomic ribozymes to facilitate the replication of i

47 inaccessible regions of both the genomic and antigenomic ribozymes were revealed by cleavage in Fe(II

48 idates both viral genomic RNA (vRNA) and the antigenomic RNA (cRNA), but not viral mRNA.

49 In contrast, the synthesis of the 1.7-kb HDV antigenomic RNA appears not to be dependent on pol II.

50 he mechanisms of synthesis of genomic versus antigenomic RNA are different.

51 V-infected woodchucks showed that 96% of the antigenomic RNA but only 50% of the genomic RNA was circ

52 randed-RNA-adenosine deaminase) can edit HDV antigenomic RNA in vitro.

53 The antigenomic RNA labeling was alpha-amanitin resistant an

54 Editing at the amber/W site of HDV antigenomic RNA leads to the production of the longer fo

55 ting at adenosine 1012 (amber/W site) in the antigenomic RNA of hepatitis delta virus (HDV) allows tw

56 The antigenomic RNA of hepatitis delta virus (HDV) can form

57 ein (N) tightly encapsidates the genomic and antigenomic RNA of RV to form the viral ribonucleoprotei

58 stance to amanitin inhibition of genomic and antigenomic RNA relative to mRNA may not reflect a diffe

59 a trans-acting ribozyme derived from the HDV antigenomic RNA self-cleaving element was established fr

60 d cells, while the complementary plus-strand antigenomic RNA sequences are present in both the nuclei

61 contrast, siRNA targeted against genomic and antigenomic RNA sequences had no detectable effect on th

62 subgenomic mRNA and full-length genomic and antigenomic RNA species in two different processes terme

63 e HDV genomic RNA strand, replication of the antigenomic RNA strand requires multiple types of posttr

64 plication, especially for replication of the antigenomic RNA strand to form the genomic RNA strand.

65 us ribozyme are derived from the genomic and antigenomic RNA strands of the human hepatitis delta vir

66 creased the ability of the genome to undergo antigenomic RNA synthesis and accumulation and/or altere

67 fied model for the regulation of genomic and antigenomic RNA synthesis.

68 t a rate roughly 30-fold higher than that of antigenomic RNA synthesis.

69 o inhibit HDV genomic RNA synthesis from the antigenomic RNA template.

70 However, the ratio of viral genomic or antigenomic RNA to mRNA was reduced in rA2DeltaM2-2-infe

71 In addition, antigenomic RNA transcripts can undergo 5' capping, 3' p

72 This study focused on the processing of antigenomic RNA transcripts.

73 ll-length cDNA of RSV strain A2 (subgroup A) antigenomic RNA was modified such that the entire SH gen

74 y, the synthesis of the full-length (1.7-kb) antigenomic RNA was not affected by alpha-amanitin to a

75 Cross-links in cis and trans forms of the antigenomic RNA were generated using the photoactivatabl

76 encapsidation and the synthesis of mRNA and antigenomic RNA, further confirming that terminal comple

77 However, the synthesis of antigenomic RNA, including both the 1.7-kb HDV RNA and t

78 In the ribozyme of hepatitis delta virus antigenomic RNA, two short duplexes, P2 and P2a, stabili

79 he genomic RNA and its exact complement, the antigenomic RNA, undergo ribozyme cleavage and RNA ligat

80 ement involved in the synthesis of the HPIV3 antigenomic RNA.

81 editing activity at the amber/W site in the antigenomic RNA.

82 inosine (I) within the HDAg-S amber codon in antigenomic RNA.

83 conserved between the 3' ends of genomic and antigenomic RNA.

84 sonchus yellow net virus (SYNV) genomic and antigenomic RNAs and the encoded polymerase proteins.

85 considered more likely that the genomic and antigenomic RNAs are resistant because their location wi

86 nt viruses from cloned cDNAs prepared to the antigenomic RNAs both of the minimally passaged virus (H

87 Circular 1,679-nt genomic and antigenomic RNAs of human hepatitis delta virus (HDV) ca

88 The 3' termini of the genomic and antigenomic RNAs of human respiratory syncytial virus (R

89 h replication and transcription, whereas the antigenomic RNAs serve only as templates for replication

90 aviruses encapsidates both viral genomic and antigenomic RNAs, although only the genomic viral RNA (v

91 d within the 5' half of both the genomic and antigenomic RNAs.

92 mRNA but had less effect on HDV genomic and antigenomic RNAs.

93 of hantavirus encapsidates viral genomic and antigenomic RNAs.

94 of initiation ability comparable to that of antigenomic RNAs.

95 plasmids which transcribe the virus S and L antigenomic RNAs.

96 its interactions with the viral genomic and antigenomic RNAs.

97 t has also been recovered from a full-length antigenomic-sense cDNA that did not conform to the "rule

98 mbranes and contain full-length genomic- and antigenomic-sense RNAs.

99 ased on a plasmid containing the full-length antigenomic sequence of CDV(OS).

100 The ribozyme self-cleavage site in the antigenomic sequence of hepatitis delta virus (HDV) RNA

101 he L3 central hairpin loop isolated from the antigenomic sequence of the hepatitis delta virus ribozy

102 s a U in the genomic sequence and a C in the antigenomic sequence.

103 g self-cleaving sequences in the genomic and antigenomic sequences of the viral RNA.

104 cific Pol II DNA-directed transcripts of HDV antigenomic sequences.

105 The antigenomic strand consisted of seven open reading frame

106 strand selectivity, we demonstrate that the antigenomic strand of HCV is not a viable RNAi target du

107 shown that the replications of genomic- and antigenomic-strand HDV RNAs have different sensitivities

108 RNA synthesis characteristics of genomic and antigenomic strands, thus identifying this nucleotide as

109 different signaling abilities of genomic and antigenomic strands.

110 ear specific signal sequences located on the antigenomic template.

111 e ability of viral RNAs (vRNAs) (genomic and antigenomic) to interact with the nucleocapsid protein (

112 self-cleavage by closely related genomic and antigenomic versions of a ribozyme.

113 The genomic and antigenomic versions of HDV contain a ribozyme that unde