ライフサイエンスコーパス: apicoplast

1 as a non-photosynthetic plastid known as the apicoplast.

2 ight target additional processes outside the apicoplast.

3 xa harbour a chloroplast-like organelle, the apicoplast.

4 l interactions with multiple proteins in the apicoplast.

5 n algal-originated plastid referred to as an apicoplast.

6 al endomembrane targeting from the PV to the apicoplast.

7 ) predicted to target to the unique parasite apicoplast.

8 cronemes, rhoptries, dense granules, and the apicoplast.

9 proposed to target protein synthesis in the apicoplast.

10 anisms possess a relict plastid known as the apicoplast.

11 synthetic plastid of algal origin termed the apicoplast.

12 e parasites harbor a peculiar organelle, the apicoplast.

13 ains a non-photosynthetic plastid called the apicoplast.

14 in the nucleus, cytoplasm, mitochondrion and apicoplast.

15 an elongation factor for translation in the apicoplast.

16 parasites contain a relict chloroplast, the apicoplast.

17 d to the plastid-like organelle known as the apicoplast.

18 such as the chloroplast-like organelle, the apicoplast.

19 d to the plastid-like organelle known as the apicoplast.

20 acid synthesis pathway, which resides in the apicoplast.

21 sm and one localized to the membranes of the apicoplast.

22 alidated protein import factor identified in apicoplasts.

23 ceramides, and cholesterol) were detected in apicoplasts.

24 as supported by filipin staining of isolated apicoplasts.

25 inositides in targeting membrane vesicles to apicoplasts.

26 riori, two prokaryotic-like organelles, the 'apicoplast' (a non-photosynthetic plastid) and the mitoc

27 in the cytoplasm and a second protein in the apicoplast, a chloroplast remnant organelle involved in

28 for multiple antimalarial antibiotics is the apicoplast, a chloroplast-like organelle of uncertain fu

29 The discovery of the apicoplast, a chloroplast-like organelle, presents drug

30 The apicoplast, a non-photosynthetic plastid of apicomplexan

31 ompartments: the mitochondrion, cytosol, and apicoplast, a plastid acquired by secondary endosymbiosi

32 tra-erythrocytic compartments, including the apicoplast, a plastid-like organelle.

33 enase (PDH) complex that is localized to the apicoplast, a specialized quadruple membrane organelle,

34 length and C-terminal fragments of T. gondii apicoplast ACC as well as C-terminal fragments of the cy

35 itution, Leu to Ile, makes Toxoplasma gondii apicoplast ACCase resistant to haloxyfop and clodinafop.

36 The apicoplast also provides a unique system to study the ce

37 The apicoplast (an intracellular organelle) contains an appr

38 Unexpectedly, FLN was found in the apicoplast, an essential chloroplast-like organelle.

39 and survival and partially localizes to the apicoplast, an indispensable organelle in apicomplexans.

40 nuclear-encoded proteins are targeted to the apicoplast, an organelle involved in fatty-acid and isop

41 results confirm the essential nature of the apicoplast and explain the inhibition of parasite growth

42 modium and Toxoplasma, the parasite lacks an apicoplast and its genome, and possesses a degenerate mi

43 encodes an SSB protein that localizes to the apicoplast and likely functions in the replication and m

44 romosomes and 12 plasmids from bacteria, the apicoplast and mitochondrion of Plasmodium falciparum an

45 lization of the two LipDHs to the parasite's apicoplast and mitochondrion, respectively, was shown by

46 Analysis of more than 1,100 mitochondrial, apicoplast and nuclear gene sequences from chimpanzees a

47 However, import into the apicoplast and processing to GFP does not occur until 18

48 ng mitosis, producing parasites that lack an apicoplast and siblings containing a gigantic, nonsegreg

49 The apicoplast and the mitochondrion of Apicomplexa cooperat

50 presence of lipoylated proteins in both, the apicoplast and the mitochondrion of T. gondii.

51 ved to be the most conserved function of the apicoplast, and fosmidomycin, a specific inhibitor of th

52 ies confirmed kitasamycin action against the apicoplast, and in vivo activity was observed in a murin

53 bilinogen synthase (PBGS) resides within the apicoplast, and phylogenetic analysis indicates a plant

54 enome is predicted to harbour genes for both apicoplast- and cytosol/endoplasmic reticulum-targeted p

55 The only DNA polymerase found in the apicoplast (apPOL) was putatively acquired through horiz

56 ancestral eukaryotic algal precursor of the apicoplast are also detectable in its genome.

57 ibody demonstrates that the ends of dividing apicoplast are closely linked to the centrosomes.

58 s likely that fatty acids synthesized in the apicoplast are ultimately incorporated into membrane pho

59 Apicoplasts are essential for parasite growth and must c

60 All resulting rpo protein trees placed apicoplast as a sister to Euglena within the green linea

61 PfClpR was localized in the P. falciparum apicoplast as is the case of PfClpP.

62 ng FASII and other pathways localized in the apicoplast as potential drug targets to prevent malaria

63 These data imply that AZ acts on the apicoplast bacterial-like translation machinery and iden

64 cipate in transit peptide degradation in the apicoplast based on its preference for basic residues at

65 er protein knockout also leads to defects in apicoplast biogenesis and a consequent loss of the organ

66 FtsH1 is the first novel factor required for apicoplast biogenesis identified in a phenotypic screen.

67 conjugate that targets the PfDXR involved in apicoplast biogenesis inhibits parasite growth and that

68 alciparum involved in chloroquine transport, apicoplast biogenesis, and phospholipid biosynthesis.

69 TgDrpA is essential for parasite growth and apicoplast biogenesis.

70 cular trafficking likely plays a role in the apicoplast biogenesis.

71 aving a novel mechanism-of-action inhibiting apicoplast biogenesis.

72 uence are required to target proteins to the apicoplast but the pathway by which proteins are transpo

73 t that in tissue culture, translation in the apicoplast can be diminished, but during an animal infec

74 to develop a detailed mechanistic picture of apicoplast cell biology.

75 ng this method is available online at http://apicoplast.cis.upenn.edu/pclr/.

76 In addition, the apicoplast contains a biotinylated protein, consistent w

77 organelles (mitochondria, chloroplasts), the apicoplast contains proteins that are encoded in the nuc

78 Derived from secondary endosymbiosis, the apicoplast depends on novel, but largely cryptic, mechan

79 We engineered a conditional null mutant in apicoplast Der1, the putative pore of the apicoplast ERA

80 The malarial "apicoplast" derived from an algal plastid, has stimulate

81 Apicoplast division depends on association with the mito

82 ls appear to generate the force required for apicoplast division in Toxoplasma gondii.

83 Apicoplast division is tightly associated with nuclear a

84 s base-mispairing and preferentially inhibit apicoplast DNA replication.

85 complete nucleotide sequence of a Plasmodium apicoplast DNA.

86 a G76V mutation in a conserved region of the apicoplast-encoded P. falciparum ribosomal protein L4 (P

87 in apicoplast Der1, the putative pore of the apicoplast ERAD complex, and found that loss of Der1(Ap)

88 The apicoplast exists in most members of the phylum Apicompl

89 our study demonstrates a direct link between apicoplast FAS II functions and parasite survival and pa

90 Most importantly, in vivo knockdown of apicoplast FAS II in a mouse model results in cure from

91 However, the specific biological roles of apicoplast FAS II remain elusive.

92 ide evidence for a direct role for TgDrpA in apicoplast fission.

93 ficient method for preparing highly purified apicoplasts from red blood cell parasite stages and the

94 Our results directly link apicoplast function with parasite survival, validating t

95 We demonstrate that the apicoplast GatAB-catalyzed reaction is essential to the

96 enome represents the first example where all apicoplast genes are encoded on one DNA strand.

97 nome sequence, we attempted to reexamine the apicoplast genome evolution and performed phylogenetic r

98 re, we report the third complete sequence of apicoplast genome from the intestinal coccidian Eimeria

99 Here, the apicoplast genome of the rodent malaria parasite Plasmod

100 sed of highly diverse protists, the complete apicoplast genome sequences have only been determined fr

101 solates we discovered point mutations in the apicoplast genome that are close to known mutations that

102 hat replication of the apicomplexan plastid (apicoplast) genome in Toxoplasma gondii tachyzoites can

103 Here we analyse the mitochondrion and apicoplast genomes of 711 Plasmodium falciparum isolates

104 ion of the first enzyme in this pathway, the apicoplast glutamyl-tRNA synthetase (GluRS).

105 t cross the four membranes that surround the apicoplast; however, experimental data discriminating th

106 P. falciparum GatA and GatB subunits to the apicoplast in blood stage parasites and demonstrated tha

107 lized the Plasmodium berghei ortholog to the apicoplast in blood stage parasites but could not delete

108 hydrogenase complex which occurs only in the apicoplast in P. falciparum.

109 synthesis is the only essential role of the apicoplast in Plasmodium erythrocytic stages.

110 If the apicoplast indeed originated from a red ancestor, the gr

111 The malaria parasite Plasmodium falciparum apicoplast indirect aminoacylation pathway utilizes a no

112 e in defining the metabolic functions of the apicoplast, information on the composition and biogenesi

113 tetracycline and rifampicin, that target the apicoplast inhibited LS development, identifying FASII a

114 Here we explore how the apicoplast interacts with its 'host' to secure building

115 a platform to dissect the integration of the apicoplast into parasite metabolism, especially its post

116 The apicoplast is a distinctive organelle associated with ap

117 The apicoplast is a relict plastid essential for viability o

118 Evidence of an apicoplast is absent, but genes associated with apical c

119 The apicoplast is home to a 1-deoxy-D-xylulose-5-phosphate (

120 The apicoplast is indispensable and an attractive drug targe

121 on across the four membranes surrounding the apicoplast is mediated by an N-terminal bipartite target

122 The data suggest that: (i) import into the apicoplast is stage regulated and (ii) the PTS can signa

123 The apicoplast is the product of an ancient endosymbiosis be

124 within the chromosomally encoded organellar (apicoplast) isoleucyl-tRNA synthetase.

125 nd immunoprecipitation studies indicate that apicoplast Kae1 and its partners interact specifically w

126 32 and Cgi121 as in other organisms, whereas apicoplast Kae1 makes novel interactions with multiple p

127 Apicoplasts lack the conserved machinery that divides ch

128 he AZ-resistant 7G8 line, the bacterial-like apicoplast large subunit ribosomal RNA harbored a U438C

129 G-->U point mutation at position 1857 of the apicoplast large-subunit rRNA, whereas no mutation was i

130 etoacid dehydrogenase complexes and that the apicoplast LipDH is an integral part of the pyruvate deh

131 lasm, and stable isotope labeling shows some apicoplast lipids are generated de novo by the organelle

132 iquid chromatography MS analyses of isolated apicoplast lipids indicated significant differences comp

133 ging drives mitochondrial lipoylation, while apicoplast lipoylation relies on biosynthesis.

134 oplasma gondii, fatty acid synthesis via the apicoplast-localized FASII is essential for pathogenesis

135 The apicoplast-localized fatty acid synthesis (FAS II) pathw

136 e, we demonstrate that T. gondii Tic22 is an apicoplast-localized protein, essential for parasite sur

137 ed with antibodies against the E2 subunit of apicoplast-localized pyruvate dehydrogenase (PDH).

138 Genetic disruption of the apicoplast-localized type II fatty-acid synthase resulte

139 An apicoplast-located fatty acid synthesis is dispensable i

140 similar to those that target proteins to the apicoplast lumen.

141 Whereas the apicoplast maintains a small genome, the bulk of its pro

142 Little is known about apicoplast membrane proteins, much less their sorting me

143 , when tagged with HA, localized to multiple apicoplast membranes in T. gondii.

144 The presence of cholesterol in apicoplast membranes was supported by filipin staining o

145 e ancestral host that gave rise to the (red) apicoplast might have already contained some primary gre

146 ring an animal infection, translation in the apicoplast must be fully functional.

147 -localize with the FAS II enzyme FabI in the apicoplast of liver stages but are not significantly exp

148 olocalize with the FAS II enzyme FabI in the apicoplast of liver stages but are not significantly exp

149 pR do not form a stable heterocomplex in the apicoplast of P. falciparum.

150 Strikingly the ACP is that of the apicoplast of Plasmodium falciparum (the protozoan that

151 n to target green fluorescent protein to the apicoplast of T. gondii.

152 In cyanobacteria, plant plastids, and the apicoplast of the genus Plasmodium, a noncatalytic paral

153 oplast and in the inner two membranes of the apicoplasts of the apicomplexan parasites, Toxoplasma go

154 the mitochondrion, we show that the plastid (apicoplast) of the obligate intracellular protozoan para

155 loped a high-throughput screen targeting the apicoplast organelle of Plasmodium falciparum.

156 Apicoplast phosphatidylinositol and other phospholipids

157 Gln-tRNA(Gln) biosynthesis in the Plasmodium apicoplast proceeds via an essential indirect aminoacyla

158 und that loss of Der1(Ap) results in loss of apicoplast protein import and subsequent death of the pa

159 Here we present genetic evidence that apicoplast protein import depends on elements derived fr

160 oss of TgTic20 leads to severe impairment of apicoplast protein import followed by organelle loss and

161 ibit translation in prokaryotes also inhibit apicoplast protein synthesis and are sometimes used for

162 Apicoplast protein synthesis is a validated drug target

163 that we hypothesized would be essential for apicoplast protein synthesis.

164 Nuclear encoded apicoplast proteins are targeted to the organelle by a b

165 asts, suggesting that blocking production of apicoplast proteins causes the 'delayed-death effect'.

166 ng of the transit peptide of nuclear-encoded apicoplast proteins requires plastid-associated activity

167 educed lipoylation of mitochondrial (but not apicoplast) proteins.

168 The mutation is present in all the apicoplast rDNA copies (an estimated 12 per organelle),

169 avage by FLN of the transit peptide from the apicoplast-resident acyl carrier protein supports this i

170 us polymorphisms in fd (ferredoxin), arps10 (apicoplast ribosomal protein S10), mdr2 (multidrug resis

171 line) argue that these drugs also target the apicoplast ribosome.

172 its partners interact specifically with the apicoplast ribosomes and with proteins involved in ribos

173 Taking advantage of the ability to isolate apicoplast segregation mutants, we also demonstrated tha

174 Together, these data indicate an expanded, apicoplast-specific role for Kae1 in the parasite.

175 arasite survival and protein import into the apicoplast stroma.

176 These progeny inherit nonfunctional apicoplasts, suggesting that blocking production of apic

177 plastids, were not detected in P. falciparum apicoplasts, suggesting that these glycolipids are a hal

178 TgDrpA) localizes to punctate regions on the apicoplast surface.

179 d that the carboxyltransferase domain of the apicoplast T. gondii ACC is the target for this class of

180 However, the predicted apicoplast-targeted lysophosphatidic acid acyltransferas

181 lts suggest that P. yoelii has an incomplete apicoplast-targeted phosphatidic acid synthesis pathway

182 nthesized fatty acids are being utilized for apicoplast-targeted phosphatidic acid synthesis, the pho

183 Our research shows that apicoplast-targeted Plasmodium yoelii glycerol 3-phospha

184 One such pathway is apicoplast-targeted type II fatty acid synthesis, which

185 Apicomplexa retain a plastid organelle (the apicoplast) that was derived from an endosymbiotic relat

186 n parasites harbor a relict chloroplast, the apicoplast, that is critical for their survival.

187 Apicoplasts thus contain an atypical melange of lipids s

188 struct of pfACP containing an amino-terminal apicoplast transit peptide, was not a substrate for pfMC

189 mutants provide direct genetic evidence that apicoplast translation is the target for clindamycin in

190 ndiscriminating because it glutamylates both apicoplast tRNA(Glu) and tRNA(Gln), determined its kinet

191 Epitope tagged TgEFG localized to the apicoplast, via a non-canonical targeting signal, where

192 In particular, apicoplasts were highly enriched in phosphatidylinositol

193 Apicoplasts were prepared from transgenic parasites expr

194 oyl transferase is present in the parasite's apicoplast, whereas the second pathway consisting of lip

195 contain an essential plastid organelle, the apicoplast, which is a key anti-parasitic target.

196 rbor a single nonphotosynthetic plastid, the apicoplast, which is essential for parasite survival.

197 asma gondii contain a primitive plastid, the apicoplast, whose genome consists of a 35-kb circular DN