1 ed C6 cell cultures contained high levels of appican and was a significantly better substrate for the

2                                              Appicans are secreted or cell-associated brain chondroit

3 rt that rat C6 glioma cells transfected with appican displayed a dramatic change in their phenotypic

4 esion to ECM was independent of the level of appican expression of the plated cells.

5   Together, these data suggest that secreted appican incorporates into ECM and promotes adhesion of n

6     Extracellular matrix (ECM) prepared from appican-transfected C6 cell cultures contained high leve

7 er the same conditions, however, <20% of the appican-transfected C6 cells were dissociated from their

8                       Conditioned media from appican-transfected C6 cultures failed to promote cell a

9                                     ECM from appican-transfected C6 cultures stimulated adhesion of o

10                                              Appican-transfected cells lost the round appearance of t

11                                 In contrast, appican-transfected fibroblast cells showed no morpholog

12 ed from their substrate, suggesting that the appican-transfected glia cells attach more avidly to the