ライフサイエンスコーパス: apyrase

1 NOate, or increased ectonucleotidase levels (apyrase).

2 y preincubation with oxidized ATP, KN62, and apyrase.

3 ct was rescued by intratracheal injection of apyrase.

4 ase activity or proper folding of this human apyrase.

5 ntrations of ADP, in contrast to R. prolixus apyrase.

6 EGTA or by removal of extracellular ATP with apyrase.

7 recently discovered mammalian lysosomal endo-apyrase.

8 B6 and CD39L3) is a membrane-associated ecto-apyrase.

9 cto-apyrase and the tunicamycin-treated ecto-apyrase.

10 codes the first reported human secreted ecto-apyrase.

11 TXA2 receptor (SQ29548), or by intratracheal apyrase.

12 tches the value found for Lutzomyia salivary apyrase.

13 e hydrolase of Toxoplasma gondii is a potent apyrase.

14 t in the glandular cells containing the ecto-apyrase.

15 mune cells suggests that CD39 may be an ecto-apyrase.

16 eversed by removing ATP by centrifugation or apyrase.

17 micro M), close to the values obtained with apyrase.

18 ation, or the presence of the ADP scavenger, apyrase.

19 nt of P2X7 and unaffected by incubation with apyrase.

20 2) receptor knockdown or ATP hydrolysis with apyrase.

21 ysteine, and reduced by the exogenous ATPase apyrase.

22 of the EGFR near wounds is not sensitive to apyrase.

23 ogated by eliminating ATP in the medium with apyrase.

24 nstructed constitutively expressing the GS52 apyrase.

25 evelopment appeared normal in the absence of apyrases.

26 , suggesting that they might encode secreted apyrases.

27 antiaggregatory effects of the administered apyrases.

28 Apyrase (10 units/ml), which rapidly hydrolyzes 5' nucle

29 tosolic adenosine triphosphate (ATP) (3 U/mL apyrase; -12.58 +/- 1.45 pA/pF), and by the putative PKC

30 ith a high concentration of the nucleotidase apyrase (17 +/- 5 pA/pF for 10 IU/ml and 11 +/- 3 pA/pF

31 ical perfusion of the ATP diphosphohydrolase apyrase (2 U ml(-1) ), or the ATPase inhibitor ARL67156

32 Such activity was blunted by apyrase, 2'-deoxy-N6-methyladenosine 3',5'-bisphosphate

33 We found that the CD39 mimic apyrase (250 U/kg) decreased and knockout or pharmacolog

34 hibited in the presence of the ATP hydrolase apyrase (3 units/ml) or by exposure to the P2 receptor b

35 ,-4'-disulfonic acid (100 microm) as well as apyrase (5 units/ml) attenuated hypoxia- and ATP-induced

36 means of the following secreted exoenzymes: apyrase, 5'-nucleotidase, and adenosine deaminase.

37 Apyrase (500 units ml(-1)), an ATP hydrolysing enzyme, d

38 amin (100 microm), PPADS (20-50 microm), and apyrase (80 U/ml), in contrast, substantially reduced wa

39 roduced when the cells were coincubated with apyrase, a highly effective ATP scavenger.

40 Apyrase, a nucleotide-degrading enzyme, suppressed and a

41 also attenuated in monocytes incubated with apyrase, a potent scavenger of extracellular ATP.

42 high pressure liquid chromatography and the apyrase active band from chromatofocusing gels.

43 ceptors, being 93 microm;(d) measured latent apyrase activities in the above vesicles, suggesting the

44 Chemicals that suppress apyrase activity also inhibit gravitropic curvature and,

45 detected, brains from these mice lacked both apyrase activity and CD39 immunoreactivity.

46 and that only a fully glycosylated CD39 has apyrase activity and is localized at the cell surface.

47 TP into the growth medium and suppression of apyrase activity by antiapyrase antibodies or by inhibit

48 Finally, we show that CD39 indeed has ecto-apyrase activity by expression in COS-7 cells.

49 he cytoplasmic domain of Ynd1p regulates its apyrase activity in the Golgi lumen.

50 his report, we show that an ecto-(Ca2+,Mg2+)-apyrase activity is present on EBV-transformed B cells,

51 hese findings, we observed that the salivary apyrase activity of L. longipalpis is indeed similar to

52 A similar increase in the apyrase activity of Ynd1p was found in a vma1Delta mutan

53 coincidence between CD39 expression and ecto-apyrase activity on immune cells suggests that CD39 may

54 igen 39 (CD39), a molecule now known to have apyrase activity.

55 idates the unusual calcium-dependence of its apyrase activity.

56 ing cells with extracellular ATP scavengers [apyrase + adenosine deaminase] versus 95 +/- 12% survivi

57 ilies of phosphatases (inactive), CD-39-type apyrase, adenosine deaminases, and esterases.

58 Histologic studies showed that apyrase administration abrogated local platelet aggregat

59 A single dose of apyrase administration before IRI protects from both acu

60 Apyrase administration significantly prolonged graft sur

61 e motor, while ATP, ATP gamma S, AMPPNP, and apyrase all induce a shift toward tighter binding states

62 Strikingly, deciliation, BAPTA-AM, and apyrase also blocked the flow-dependent increase in endo

63 Apyrase (an agent of ADP degradation), suramin (a genera

64 Suramin, apyrase (an ATP-hydrolyzing enzyme), and TTX substantial

65 In addition, the inhibitory effects of apyrase, an ADP scavenger, on spreading and tyrosine pho

66 Local injection of apyrase, an ATP-hydrolyzing enzyme, inhibited cell recru

67 lasts in the absence of ATP was inhibited by apyrase, an ecto-ATPase and by suramin, an antagonist of

68 TP and ADP were abolished in the presence of apyrase, an ectonucleotidase.

69 ne marrow reconstitution and provision of an apyrase, an enzyme that hydrolyzes nucleoside tri- and d

70 d [Ca(2+)]i rise was partially attenuated by apyrase, an enzyme that inactivates extracellular ATP, a

71 P2 antagonists or apyrase, an enzyme which hydrolyzes nucleotides includin

72 Moreover, using apyrase, an extracellular ATP scavenger, we showed that

73 Apyrase and antagonists of platelet purinergic receptors

74 nzymatic degradation of extracellular ATP by apyrase and blockade of P2Y-purinoceptors by suramin or

75 lso reduced significantly in the presence of apyrase and connexin channel inhibitors.

76 chromatography of detergent-solubilized ecto-apyrase and cross-linking of membrane-bound ecto-apyrase

77 latory volume decrease which was impaired by apyrase and facilitated by ATP or UTP.

78 ymes that use acid-labile substrates such as apyrase and glutamine synthetase.

79 e stomach 80-kDa protein isolated is an ecto-apyrase and is related to both the chicken liver and ovi

80 ts of increased CD39 activity (using soluble apyrase and mice expressing human CD39 transgene) on acu

81 , including potato tuber (Solanum tuberosum) apyrase and parasite ecto-ATPase, are not affected by de

82 nreported (to our knowledge) linkage between apyrase and phosphate transport.

83 nsients in submucous neurons were reduced by apyrase and prevented by blocking the P2Y1 R with MRS 21

84 ere induced by the soluble ecto-nucleotidase apyrase and the P2 receptor inhibitor suramin.

85 ast to the enzymatically deglycosylated ecto-apyrase and the tunicamycin-treated ecto-apyrase.

86 displacement was reversed by the addition of apyrase and was specific to chromatin templates.

87 robenecid, and (10)Panx1), ectonucleotidase (apyrase), and purinergic receptor inhibitors (suramin an

88 ation (5 mm EGTA), ATP depletion (4 units/ml apyrase), and the protein kinase C (PKC) inhibitors chel

89 his increase in motility is not sensitive to apyrase, and apyrase does not detectably inhibit healing

90 adation was significantly inhibited by EDTA, apyrase, and the proteasome inhibitors hemin and MG132.

91 ity within xenografts by infusion of soluble apyrases, and thereby validate the importance of local A

92 Anti-ecto-apyrase antibody detected a single 80-kDa band (putative

93 elements from the Maltase-like I (MalI) and Apyrase (Apy) genes were cloned so as to direct the expr

94 lases), and two nearly identical Arabidopsis apyrases, APY1 and APY2, appear to share this function.

95 Two closely related apyrases (APYs) in Arabidopsis (Arabidopsis thaliana), A

96 Apyrases are non-energy-coupled nucleotide phosphohydrol

97 sults suggest that the ecto-ATPases and ecto-apyrases are part of, or closely related to, the actin s

98 , and this [Ca2+]i increase was inhibited by apyrase as well.

99 ressed the protein and message level of both apyrases at least as rapidly as it inhibited hypocotyl g

100 we previously identified two highly similar apyrases, AtAPY1 and AtAPY2.

101 y of spontaneous wave generation by 53%, and apyrase (ATP-hydrolyzing enzyme) reduces frequency by 95

102 tion of exogenous potato (Solanum tuberosum) apyrase (ATPase) decreased ROS activity, suggesting that

103 zation of extracellular ecto-ATPase and ecto-apyrase (ATPDase) in adult chicken gizzard and stomach b

104 ces confirmed that pretreatment with oATP or apyrase attenuated cytokine-mediated induction of this t

105 Suramin, Cibacron blue 3GA, and apyrase attenuated hypoxia-induced ERK1/2 activation and

106 terminal sequence of the 80-kDa stomach ecto-apyrase band (which reacted with anti-ecto-ATPDase antib

107 the prostaglandin E(1) or the ADP scavenger apyrase but was prevented by the divalent cation chelato

108 icant sequence similarity to any other known apyrases, but homologous sequences have been found in th

109 hibited by removal of extracellular ATP with apyrase, by inhibition of the P2X(7) receptor with A4380

110 +) that was blocked by MK-801, by the ATPase apyrase, by the P2Y(1) receptor antagonist MRS2179 and b

111 a presented show that expression of the GS52 apyrase can enhance nodulation in L. japonicus and point

112 xy-D-glucose with oligomycin or perfusion of apyrase), can be restored with phosphatidylinositol 4,5-

113 These results clearly suggest that GS52 ecto-apyrase catalytic activity is critical for the early B.

114 substrate specificity characteristic of the apyrase category of phosphohydrolases, and its sequence

115 alysis revealed an increase in CD73 and ecto-apyrase CD39 in hypoxic epithelial cells.

116 Regulatory T cells (known as "Treg") express apyrases (CD39) and ecto-5'-nucleotidase (CD73) and cont

117 esults were obtained with another human ecto-apyrase, CD39, suggesting that the importance of glycosy

118 This apyrase cluster was mapped to linkage group seven.

119 7 to alanine yielded a poorly expressed ecto-apyrase completely devoid of nucleotidase activity.

120 We show that mutations in apyrase conserved region 1 (ACR1) have two dramatically

121 cted mutagenesis of conserved amino acids in apyrase conserved regions (ACR) I and IV.

122 2) contains conserved motifs including five apyrase conserved regions (ACRs) and four conserved regi

123 ructural basis for the importance of several apyrase conserved regions for the nucleotidase activitie

124 contain two transmembrane domains and five "apyrase conserved regions" (ACR) within a large extracel

125 Four highly conserved regions (apyrase conserved regions, ACRs) have been identified in

126 ur of the conserved sequences, designated as apyrase conserved regions, are present in both ecto-ATPa

127 tion sites, conserved cysteine residues, and apyrase conserved regions.

128 were generated by replacement of exons 4-6 (apyrase-conserved regions 2-4) with a PGKneo cassette.

129 r APY1 and APY2 show that expression of both apyrases correlates with conditions that favor stomatal

130 psis, suggested that the expression of these apyrases could influence auxin transport.

131 iating platelet aggregation (i.e., Aegyptin, apyrase, D7) represent functional redundancy.

132 by canine kidney cells with the nucleotidase apyrase decreases basal arachidonic acid release and cAM

133 in motility is not sensitive to apyrase, and apyrase does not detectably inhibit healing of wounds in

134 ontains one large NH(2)-terminal hydrophilic apyrase domain, one COOH-terminal hydrophilic domain, an

135 ta suggest a critical role for the GS52 ecto-apyrase during nodulation.

136 The data suggest a role for apyrases early in the nodulation response before the inv

137 tes, but not monophosphates, thus displaying apyrase (EC 3.6.1.5) activity, was purified from salivar

138 We used apyrase, EDTA and Dowex-1 to prepare actin that is stabl

139 d by the gene uda-1, whereas the other is an apyrase encoded by the gene ntp-1.

140 es the role of extracellular nucleotides and apyrase enzymes in regulating stomatal aperture.

141 idase 1 (hSCAN-1) represents a new family of apyrase enzymes that catalyze the hydrolysis of nucleoti

142 The glycosylated ecto-apyrase exists as a homodimer in situ as assessed by bot

143 ression changes that occur in seedlings when apyrase expression is suppressed were assayed by microar

144 onstruct was measured in primary roots whose apyrase expression was suppressed either genetically or

145 arthropods, while members of the Cimex-type apyrase family have been recruited at least twice.

146 structures of a human enzyme of the soluble apyrase family in its apo state and bound to a substrate

147 (hSCAN-1) is the human homologue of soluble apyrases found in blood-sucking insects.

148 ed cDNA clone recognized both C. lectularius apyrase fractions eluted from a molecular sieving high p

149 An apyrase from pea (Pisum sativum) complements a yeast (Sa

150 wever, the recent cloning of an ecto-ATPase (apyrase) from potato tubers provides a new opportunity t

151 The human ecto-apyrase gene family consists of five reported members (C

152 Transgenic plants overexpressing an apyrase gene had reduced O2- production in response to a

153 is thaliana of either psNTP9, the garden pea apyrase gene, or AtPgp1, the A. thaliana homolog of the

154 Four putative apyrase genes were identified from the model legume Medi

155 ge group J was found to contain at least two apyrase genes.

156 tant role for the soybean (Glycine max) ecto-apyrase GS52 in rhizobial root hair infection and root n

157 The soybean apyrase, GS52, was previously characterized as an early

158 s and etiolated hypocotyls overexpressing an apyrase had faster growth rates than wild-type plants.

159 C. lectularius apyrase has no significant sequence similarity to any ot

160 The cDNA encoding a human ecto-apyrase (HB6), predicted to have seven N-linked glycosyl

161 In addition, when ATP was scavenged with apyrase, hyperoxia-induced inflammasome activation was s

162 larity to: (i) the bed bug Cimex lectularius apyrase, (ii) a 5'-nucleotidase/phosphodiesterase, (iii)

163 Removal of the extracellular ATP by apyrase in CM abolished this effect, suggesting the invo

164 ctions, but the antibody did not detect ecto-apyrase in immunolabeled gizzard cryosections.

165 ngipalpis is indeed similar to that of Cimex apyrase in its metal requirements.

166 Overexpression of the GS52 ecto-apyrase in Lotus japonicus increased the level of rhizob

167 reatment with BAPTA-AM, or upon inclusion of apyrase in the perfusion medium.

168 ior data indicate that the expression of two apyrases in Arabidopsis (Arabidopsis thaliana), APY1 and

169 Despite the redundancy of the two apyrases in rescue potential, transmission analyses sugg

170 body detected a single 80-kDa band (putative apyrase) in Western blots of both chicken gizzard membra

171 ase and cross-linking of membrane-bound ecto-apyrase, in contrast to the enzymatically deglycosylated

172 Likewise, treatment with the ATPase apyrase, in the presence of the adenosine A1 and A2 rece

173 denosine A1 receptor blockade and reduced by apyrase inactivation of nucleotidases, P2 receptor antag

174 ed homology with other ecto-ATPases and ecto-apyrases, including those from the parasitic protozoan T

175 is initial dose was followed by a continuous apyrase infusion (8.0 U/kg/hr) directly into the graft a

176 ARL67156 augmented, and apyrase inhibited, stroking-evoked Isc responses.

177 Apyrase inhibition by small molecule inhibitors reversed

178 Apyrase, inhibition of P2 receptors, or inhibition of p3

179 Treatment of untransformed plants with an apyrase inhibitor increased their sensitivity to the sam

180 Ecto-apyrase is enriched in brain postsynaptic density membra

181 esent throughout the brain suggest that ecto-apyrase is involved in regulating synaptic transmission

182 emplified by the endothelial CD39 (NTPDase1) apyrase, is a 38 kDa monomeric enzyme capable of hydroly

183 rthermore, we show that CD39-L4 is an E-type apyrase, is dependent on calcium and magnesium cations,

184 y dithiols, NTPase is one of the most potent apyrases known to date, but its physiological function r

185 d characterization of a novel mammalian endo-apyrase (LALP1) in human and mouse.

186 Functional apyrase levels in vivo were assessed by the capacity of

187 ates that the catalytic activity of the GS52 apyrase, likely acting on extracellular nucleotides, is

188 predicted isoelectric point of the putative apyrase matches the value found for Lutzomyia salivary a

189 When scavenging phosphate from xATP, apyrase mobilizes the gamma-phosphate without promoting

190 of mRNA expression of the other two putative apyrases, Mtapy2 and Mtapy3, was unaffected by rhizobial

191 on of two Arabidopsis (Arabidopsis thaliana) apyrase (nucleoside triphosphate-diphosphohydrolase) gen

192 We also partially purified the ecto-apyrase of chicken stomach, an 80-kDa membrane glycoprot

193 The effect of mutant apyrase on the transmission through the female gametophy

194 Removal of extracellular ATP by apyrase or alkaline phosphatase treatment, inhibition of

195 When extracellular ATP was hydrolyzed by apyrase or ATP/P2 receptors were blocked, injury-induced

196 d by either extracellular ATP depletion with apyrase or blockade of P2 receptors with suramin.

197 n be inhibited by the ATP-hydrolyzing enzyme apyrase or by blockers of G protein-coupled purinergic r

198 Luminal apyrase or suramin blocked the effects of forskolin but

199 icturition because intravesical perfusion of apyrase or the ecto-ATPase inhibitor ARL67156 altered re

200 Enzymatic removal of ATP and UTP (by apyrase or the expression of ectopic CD39) abrogated the

201 by the serosal, but not mucosal, addition of apyrase or the purinergic receptor antagonist PPADS.

202 on with the nonspecific pyrophosphohydrolase apyrase or with hexokinase and was specifically lost by

203 O mutation is fully penetrant and shows that apyrases play a crucial role in pollen germination.

204 related proteins," PPTSP42 and PPTSP44, and "apyrase," PPTSP36, are the proteins responsible for the

205 nding to the N-terminal sequence of purified apyrase produced a probe that allowed identification of

206 ading endogenous spinal ATP by administering apyrase produces a reduction in withdrawal behaviors.

207 ata demonstrate that local administration of apyrase prolonged discordant xenograft survival.

208 t express luciferase (LUC) from the mosquito Apyrase promoter were intrathoracically inoculated with

209 beta-glucuronidase staining in the pollen of apyrase promoter:beta-glucuronidase fusion transgenic li

210 We have shown that ecto-apyrase protein is expressed in primary neurons and astr

211 mmunohistochemical studies showing that ecto-apyrase protein is widely distributed in rat brain, as i

212 report that overexpression of either MDR or apyrase proteins resulted in increased resistance to her

213 Soluble apyrase reduced acute renal injury at 24 hours and renal

214 Soluble apyrase reduced renal ATP, adenosine diphosphate, and ad

215 by the P2Y1 blockers, MRS2176, suramin, and apyrase, reduces Ca(2+) transients and retards INP migra

216 mutagenesis within the four highly conserved apyrase regions of solCD39.

217 The two transmembrane domains of CD39 ecto-apyrase regulate the formation of fully active homotetra

218 mmunolocalization of this active mutant ecto-apyrase revealed a cellular pattern similar to that of t

219 Incubating lingual slices with apyrase reversibly blocked cell-to-cell communication be

220 The ATP/ADPase apyrase reversibly inhibited the [Ca2+]i rise induced by

221 onized extension due mainly to a decrease in apyrase's efficiency in degrading excess nucleotides pro

222 immunoreactive material detected by the anti-apyrase serum.

223 approach, we were able to redesign the human apyrase so as to enhance its ADPase activity by more tha

224 to hydrolysis of extracellular ATP levels by apyrase (soluble NTPDase).

225 rmination, confirming that the phenotype was apyrase specific.

226 In addition, apyrase strongly inhibited influenza-specific T-cell res

227 Computer modeling of the GS52 apyrase structure identified key amino acid residues imp

228 noid waves were blocked by either octanol or apyrase, suggesting that propagation might occur either

229 lts indicate that a critical step connecting apyrase suppression to growth suppression is the inhibit

230 on by azide, a more potent inhibitor of ecto-apyrases than ecto-ATPases.

231 Hematophagous arthropods secrete a salivary apyrase that inhibits platelet activation by catabolizin

232 On the other hand, apyrase, the P2Y12 receptor antagonist, AR-C67085, and i

233 was studied, using the ATP hydrolyzing agent apyrase, the purinergic receptor agonist PPADS, the calc

234 P) essentially converted the eNTPDase-3 ecto-apyrase to an ecto-ATPase (eNTPDase-2), mainly by decrea

235 n E-type ATPase essentially converts an ecto-apyrase to an ecto-NTPase.

236 The ability of apyrase to block chemokine-dependent aggregation or adhe

237 Mice were treated with anti-IL-33 or apyrase to neutralize or block IL-33 release.

238 Pre-photolysis force was minimized using apyrase to remove contaminating ATP and ADP.

239 ell death, and addition of the ATP scavenger apyrase to remove extracellular ATP released during Hst

240 evealed that two such mutants do not express apyrases to any detectable level.

241 Apyrase treatment also depressed DOR stimulation of intr

242 Apyrase treatment of translation mixtures after synthesi

243 Apyrase treatment substantially reduced the basal profib

244 nt protein kinase kinase (CaMKK) inhibition, apyrase treatment, G(q/11) antagonism, and blockade of P

245 ecific activity of the purified stomach ecto-apyrase was 75,000 micromol of Pi/mg of protein/h, and t

246 ic cardiac xenografting from guinea pigs and apyrase was administered intravenously (200 U/kg) as a s

247 The ecto-apyrase was also expressed in the presence of tunicamyci

248 The purified C. lectularius apyrase was an acidic protein with a pI of 5.1 and molec

249 in 70/sugar kinase superfamily, a human ecto-apyrase was analyzed by site-directed mutagenesis of con

250 Surprisingly, apyrase was more effective than prostacyclin and NO at l

251 In adult chicken stomach, the ecto-apyrase was observed at the apical membrane of the gland

252 of the cDNA clone with native C. lectularius apyrase was proved by immunological testing and by expre

253 motors, the nucleotide-free state induced by apyrase was the strongest binding (K(kin)d approximately

254 Apyrase was used to hydrolyze extracellular ATP, and sur

255 ther methods, the highest expression of both apyrases was in rapidly growing tissues and/or tissues t

256 A human brain E-type ATPase (HB6 ecto-apyrase) was subjected to site-directed mutagenesis to a

257 GS52, a soybean (Glycine soja) ecto-apyrase, was previously shown to be induced very early i

258 g CD39 activity with ARL67156 (inhibitor) or apyrase were also observed.

259 etic analyses of three families of arthropod apyrases were used to reconstruct the evolutionary relat

260 toenzyme ATP-diphosphohydrolase (ATPDase; an apyrase), which exerts an important thromboregulatory fu

261 In the presence of apyrase, which hydrolyzes ATP to AMP, the effect of LL37

262 This apyrase, which is distinct from the eNTPDases exemplifie

263 newly discovered human analogue of a bed bug apyrase, which we named hSCAN-1 for human soluble calciu

264 utionary relationships of salivary-expressed apyrases, which have an anti-coagulant function in blood

265 fected COS-7 cells secreted a Ca2+-dependent apyrase with a pI of 5.1 and immunoreactive material det

266 Replacing apyrase with a washing step for removal of excess nucleo

267 ryptophan 459 to alanine resulted in an ecto-apyrase with enhanced NTPase activity, but diminished ND

268 LALP1 indicated that LALP1 is indeed an endo-apyrase with substrate preference for nucleoside triphos