ライフサイエンスコーパス: assay

1 say) and cell migration (transwell migration assays).

2 G2 was investigated by a colorimetric ATPase assay.

3 e IgG anti-BP180 enzyme-linked immunosorbent assay.

4 itative evaluation, and cytokines by Luminex assay.

5 ference using a fluorogenic peptide cleavage assay.

6 a-gal-sIgE levels were assessed by ImmunoCAP assay.

7 of usual antioxidants were assessed by DPPH assay.

8 inexpensive, high sensitivity CVD biomarker assay.

9 iciency of drug conjugation with a catalytic assay.

10 as measured by using an in vitro cell uptake assay.

11 criptional activity, as judged by luciferase assay.

12 n levels in an in vitro gB/gH-gL cell fusion assay.

13 was assessed by enzyme linked immuno sorbet assay.

14 ing quantitative real time PCR and multiplex assay.

15 ay, showing full concordance with the TaqMan assay.

16 by intracellular staining and dual-secretion assay.

17 owed by testing in a fluorescence anisotropy assay.

18 an metabolism and urban water fingerprinting assay.

19 lar potencies in an ATP synthesis inhibition assay.

20 d detectable plasma HIV-1 RNA by single-copy assay.

21 icated using the single-antigen bead Luminex assay.

22 gliadine peptide using an immunofluorometry assay.

23 tiplexed to target many peptides in a single assay.

24 s to alpha4beta7 in an in vitro cell binding assay.

25 s analysed by electrophoretic mobility shift assay.

26 in water samples using nanomaterial modified assay.

27 ted by miR-124-3p with a luciferase reporter assay.

28 d a peptide-nucleic acid (PNA) hybridization assay.

29 ined by a novel CD154 T cell epitope mapping assay.

30 increasingly available from high-throughput assays.

31 cholerae biotypes in growth and biochemical assays.

32 y genetic interactions and pre-mRNA splicing assays.

33 ate paper substrates for fluorophore-labeled assays.

34 on the current and future role of HTS-based assays.

35 model membranes in a variety of biophysical assays.

36 al layer treatment, and multiplex analytical assays.

37 tocyte counts or results of mosquito-feeding assays.

38 -log reduction measured by virus infectivity assays.

39 at functional level using in vitro reporter assays.

40 ell viability, and AnnexinV-FITC/PI staining assays.

41 of prostate cancer cell lines in 3D in vitro assays.

42 /10 and BJ/92 in hemagglutination inhibition assays.

43 nscriptional activity in luciferase reporter assays.

44 ness and reproducibility among immunological assays.

45 ty were determined for each of the developed assays.

46 alidated by in vitro and in-matrix migration assays.

47 plementation of robust and large multiplexed assays.

48 e translation of this material into clinical assays.

49 iant region were analyzed with reporter gene assays.

50 tened reconstitution in competitive transfer assays.

51 ions resulting in a dataset representing 238 assays.

52 uminescence resonance energy transfer (BRET) assays.

53 t turnover of ACD11 in cell-free degradation assays.

54 In a functional assay, 1b displayed full antagonist activity with IC50 =

55 ease in anxiety as measured in an open-field assay after intraperitoneal CGRP.

56 Uniquely, the HIV(RNA/Gag) assay also allows parallel phenotyping of viral reservoi

57 s good potency in an IDO-1 human whole blood assay and also shows a very favorable ADME profile leadi

58 ORMDL3 induction were examined by transwell assay and depletion experiments, respectively.

59 show estrogenic activity in the uterotrophic assay and did not alter puberty in male and female rats

60 The live/dead staining, cell proliferation assay and immunohistochemistry study of human corneal ep

61 LT3 inhibition by plasma inhibitory activity assay and indicated improved overall survival and signif

62 ces EBOV polymerase activity in a minigenome assay and TRIM6 knockout cells have reduced replication

63 r of C5 cleavage, using in vitro whole-blood assays and an in vivo baboon model of Escherichia coli s

64 critical for the development of lateral flow assays and analytical devices based on paper microfluidi

65 We determined Fic in multiple cellular assays and cell types representing different targets fro

66 Validation through enzyme assays and customized (13) C metabolite profiling showed

67 nsive WGS assay has replaced seven molecular assays and has resulted in resistance profiles being rep

68 Using adhesion/invasion assays and immunofluorescent and transmission electron m

69 was then compared using in vitro transporter assays and in vivo microdialysis in rat nucleus accumben

70 charomyces cerevisiae Using cell-free fusion assays and light microscopy, we find that GTPase activat

71 sing macroscopic rubidium ((86)Rb(+)) efflux assays and patch-clamp electrophysiology.

72 We developed molecular assays and portable optical imaging designs that permit

73 Our development simplifies complicated assays and the transportation of reagents and mitigates

74 lly reduced HHSteC proliferation (AlamarBlue assay) and cell migration (transwell migration assays).

75 y, to endomysium using an immunofluorescence assay, and antibodies to a deamidated gliadine peptide u

76 to DST for eight drugs, confirmatory Wayne's assay, and whole-genome sequencing.

77 erformed marker analysis, cell proliferation assays, and genetic lineage tracing to define the lineag

78 nction-separating alleles, live-cell spindle assays, and in vitro biochemical analyses, we show that

79 affinity binding to LRP6 in in vitro binding assays, and inhibition of LRP6 or critical signaling cas

80 Using site-directed mutagenesis, kinetic assays, and quantitative mass spectrometry, we precisely

81 d increased CTCF binding in promoter-binding assays, and risk allele carriage diminished transcriptio

82 All cell migration and wound healing assays are based on the inherent ability of adherent cel

83 Serological assays are not routinely used in the diagnosis because m

84 g cells with simple, sensitive and selective assays are significantly interesting.

85 existing LFA formats (predominantly sandwich assays) are not suitable for small molecule targets.

86 Enzyme-linked immunospots, cytotoxicity assays as well as adoptive transfer in NOD/SCID/IL2Rgamm

87 oth muscle actin-positive blood vessels were assayed at postoperative day 7 by colony formation and i

88 l conditions and identified by molecular PCR assay based on the ITS-5.8S rDNA.

89 Finally, a novel multiplex PCR assay, based on random amplified polymorphic DNA (RAPD)

90 RBCs were collected and assayed before (n = 327), 14 days (n = 82), 49 days (n =

91 A competition assay between GDP and GDP analogues in the GpppA formati

92 In the absence of vivax invasion assays, binding-inhibitory activity of antibodies has be

93 variable stimulatory effects in the cellular assay, but not in vitro Sequencing analyses further demo

94 no impact on wound healing in normal scratch assays, but after subjecting the cells to mechanical str

95 We initially evaluated the assay by testing reference Leishmania isolates and compa

96 This powerful single-molecule assay can be highly useful in diagnostic applications us

97 Furthermore, the assay can be used for fine-tuning reaction mixtures of v

98 This splicing assay can be used to explore in detail how HIV-1 splicin

99 This highly sensitive p24 assay can help improve blood safety by reducing the anti

100 Results showed the assay can rapidly detect H3N2 IAVs directly from nasal w

101 s (QACs) are exuded by germinating seeds, we assayed chemotaxis of S. meliloti towards betonicine, ch

102 an experimental landscape of mesocosms, and assayed colonization by 35 species of aquatic beetles.

103 Membrane integrity assays confirm that the voltage delivered by the wires d

104 A proximity ligation assay confirmed the endogenous Trx1-GC1 complex in cells

105 In vitro assays confirmed PhCAT2 can transport Phe, and decreased

106 This assay could enable specific diagnosis of ZIKV infection

107 Luciferase reporter assays demonstrated that high miR-375 expression reduced

108 Reporter assays demonstrated that rs9920291 shows allelic regulat

109 DNA binding and transcription assays demonstrated that the minor T allele variation at

110 Two-hybrid and pulldown assays demonstrated that UL20, but no other HSV-1 gene-e

111 be easily developed for certain enzymes, HTS assays designed to identify ligands that block protein b

112 with primary infection, the cultured ELISPOT assay detected a higher T-cell response to pp65 than to

113 e (AMC) were selected for further behavioral assays due to their temporal correlation with the behavi

114 lectronics-based Enzyme-Linked ImmunoSorbent Assay (eELISA), using a Lab-on-a-Printed Circuit Board (

115 nsitive sandwich enzyme-linked immunosorbent assay (ELISA) for PEG by tethering an anti-PEG antibody

116 elementary body enzyme-linked immunosorbent assay (ELISA) was used to investigate serum anti-CT immu

117 IN1 detection by enzyme-linked immunosorbent assay (ELISA), and cBIN1 level decreased in humans with

118 Using enzyme-linked immunosorbent assay (ELISA), we obtain seropositivity of 77%.

119 s or better than enzyme-linked immunosorbent assay (ELISA).

120 a single-analyte enzyme-linked immunosorbent assay (ELISA).

121 re quantified by enzyme-linked immunosorbent assays (ELISA) or theoretically calculated in 12 brain r

122 valuated both in enzyme-linked immunosorbent assays (ELISA).

123 hamiltonii could be used to design specific assays, enhancing its applicability for direct use in CI

124 rtheless, designing high-throughput reporter assay experiments such as massively parallel reporter as

125 xpression; while exogenous promoter-reporter assays failed to reproduce the similar outcomes.

126 man fetal testes explants called FEtal Gonad Assay (FEGA) with tissue obtained at 10 and 12 gestation

127 Here using band shift assays, fluorescence Job plots, and yeast three-hybrid a

128 d MLVA-13Bc assay is a highly discriminatory assay for B. canis genotyping, and can serve as a useful

129 C18:1PC) lipid vesicles using a fluorescence assay for gA channel activity.

130 Using an ex vivo assay for HIV-1 mRNA, we demonstrated that despite this

131 e describe the application of a standardized assay for HIV-1 RNA in multiple specimen types.

132 Here, we used a multiplexed assay for kinase specificity (MAKS) to identify the subs

133 ndividual pancreatic cancers and the optimal assay for patient selection may inform clinical trial de

134 arvae xenografts constitute a promising fast assay for precision medicine, bridging the gap between g

135 The data show the potential of our assay for rapid detection of antifungal resistance, alth

136 relation with an enzyme-linked immunosorbent assay for sample detection in patients.

137 In this study, we optimized the Xpert EBOV assay for semen samples by adding dithiothreitol.

138 protocol, and their application in an ELISA assay for sensitive diagnosis of Zika NS1 protein.

139 report the development of a high sensitivity assay for the detection of cardiac troponin I using elec

140 ped a new single-strand consensus sequencing assay for the determination of HIV mutation frequencies

141 pitation sequencing (ChIP-seq) combined with assay for transposase-accessible chromatin coupled to hi

142 report a novel adaptation of the "Repli-seq" assay for use in intact root tips of maize (Zea mays) th

143 Both maternal and cord serum samples were assayed for levels of IgG1 and IgG3 specific for MSP119,

144 splaying yellowing and inedible fruits, were assayed for the presence of phytoplasma DNA.

145 Assaying for anti-BP180 IgE increased the diagnostic sen

146 used in cell permeability and flow cytometry assays for apoptosis and proliferation.

147 ts in developing highly efficient biomimetic assays for interaction analysis and drug screening invol

148 ity, hindering the development of cell-based assays for NMDAR drug discovery.

149 Development of standardized assays for novel biomarkers toward better defining hepat

150 Targeted mass spectrometry assays for protein quantitation monitor peptide surrogat

151 constant model, and tested in a paper-based assay format using a fusion construct consisting of an r

152 in cell-based dual-luciferase reporter gene assays, functional interaction occurred between miR-18a-

153 cts blood DNA methylation in MS patients, by assaying genome-wide DNA methylation and comparing smoke

154 This single comprehensive WGS assay has replaced seven molecular assays and has result

155 The developed assay has the potential to be implemented in food testin

156 A few phenotypic assays have been described but have not been independent

157 In this manuscript, two additional assays have been developed.

158 ase chain reaction (PCR) and other molecular assays have demonstrated an association with adverse dis

159 ) assay, lactate dehydrogenase (LDH) release assay, Hoechst 33342 staining, annexin V/PI staining, an

160 Employing immunoprecipitation assays, hRETNTg(+)Tlr4(-/-) mice, and human immune cell

161 imolar concentration library, the CaR-ESI-MS assay identified 100% of ligands with affinities >500 M(

162 Interaction assays identified FDH as a potential substrate for the R

163 CMV DNA was detected using commercial artus assay in 10.7% of asthma patients, but was negative in a

164 d for their cytotoxicity by colony formation assay in cells of different BRCA2 status.

165 wi-interacting RNA (piRNA)-targeted reporter assay in Drosophila ovary somatic sheet (OSS) cells [1].

166 Smooth-muscle cells from mouse tracheas were assayed in vitro for signaling pathways.

167 nd DNA repair defects by direct and indirect assays in 12 breast cancer cell lines to estimate the sp

168 actions and in situ point mutation detection assays in preserved tumour samples can be imaged and ana

169 , we used a V(D)J passenger allele system to assay, in mouse GC B cells, sequence-intrinsic SHM-targe

170 The performance of the assay, in terms of sensitivity and reproducibility, was

171 are kits that incorporate nucleic acid-based assays, including polymerase chain reaction, isothermal

172 tion (TRAP) and in vitro luciferase reporter assay indicate that mir-92a suppresses expression of sir

173 The in vitro enzyme assays indicate that these distinct metabolic phenotypes

174 qRT-PCR assay indicated that compound 1 and DBL exposure up-regu

175 kDNA decatenation assay indicated that XWL-1-48 significantly inhibited to

176 In isolated tissue assays, inosine (1 mM) significantly decreased the ampli

177 The assay involves the use of the stable isotope dilution me

178 Our newly developed MLVA-13Bc assay is a highly discriminatory assay for B. canis geno

179 Furthermore, the presented chiral assay is able to determine the enantiomeric excess (ee)

180 This SID nanoLC-NSI/MS/MS assay is highly sensitive and specific and it requires o

181 The other, a chemical assay, is known as the Sakaguchi test and targets argini

182 By using a virus overlay assay, it was previously shown that the major AAV2 bindi

183 f a magnetic field can be used to accelerate assay kinetics.

184 2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, lactate dehydrogenase (LDH) release assay, Hoechs

185 A lateral flow assay (LFA) can provide a rapid and cost-effective means

186 ucleic acid hybridization-based lateral flow assay (LFA) holds great potential to address these limit

187 ditions, we evaluated two rapid lateral flow assays (LFA) for detection of Mycobacterium leprae-speci

188 The assay limit of detection (LOD) and dynamic range were de

189 node-positive breast cancer, the MammaPrint assay may be used in patients with one to three positive

190 While rugged and robust assays may be easily developed for certain enzymes, HTS

191 eriments such as massively parallel reporter assays (MPRAs) and similar methods remains challenging.

192 sequencing initiative using a comprehensive assay, MSK-IMPACT, through which we have compiled tumor

193 d self-containing system that allows for the assay of different analytes in complex samples.

194 Additionally, assay of liver enzymes and histology analysis of local t

195 As compared to direct assays of plasma or leukocytes, the EV detection rate wa

196 early diagnosis of CJD by using the RT-QuIC assay on CSF samples, OM samples, or both.

197 we systematically performed microchemotaxis assays on purified immune subsets including human pan-T

198 adio-ligand uptake and fluorescent transport assays on ZntB reconstituted into liposomes.

199 robalance; and motor bioactivity with ATPase assay, on a set of model surfaces, i.e., nitrocellulose,

200 Findings confirm that viral crAssphage qPCR assays perform at a similar level to well-established ba

201 antifungal resistance, although the MS-AFST assay performed at 3 h of the in vitro antifungal exposu

202 ineage and is recapitulated in developmental assays performed on OL progenitor cells purified from Th

203 ord and myelinate peripheral motor axons, we assayed perineurial glial development, maturation, and r

204 he polyclonal serum-based proximity ligation assay (polyPLA), to identify antigenic variants of subty

205 that includes lipid substrates for enzymatic assays, potent inhibitors, and chemical probes to detect

206 ke mechanism could be rather nonspecific The assay promises to be a powerful tool for the classificat

207 on, we exploited a prion conversion in vitro assay, protein misfolding cyclic amplification (PMCA), b

208 rmined LTB4, and enzyme-linked immunosorbent assays quantified tumor necrosis factor (TNF), interleuk

209 immunofluorescent staining and the PNA-Fish assay, respectively.

210 8 parasite/microL in PfLDH- and PfIDEh-based assays, respectively.

211 Results The 21-gene assay resulted in a net decrease in chemotherapy use of

212 employing immunofluorescence antibody-based assays reveal no changes in H3K4me3 levels in cells ecto

213 nt plants and electrophoretic mobility shift assay revealed sequence-specific binding of rSaWRKY1 wit

214 Biochemical assays revealed Abl phosphorylates Mical to directly amp

215 Genetic assays revealed that Abl allows growth factors and Semap

216 , coimmunoprecipitation and in vitro binding assays revealed that mortalin facilitates PP1alpha-media

217 followed by inhibitor and antibody-blocking assays revealed that the arthropod HSP70-like molecule c

218 In vitro and cell-based assays revealed that the CNAbeta1-containing holoenzyme,

219 Cell-cell fusion assays show a strain-independent failure of fusion pore

220 n vitro reconstitution and in vivo targeting assays show that SecA is necessary and sufficient to dir

221 Kinetic assays showed rapid bactericidal effect of LyeTxI agains

222 Although early in vitro enzyme assays showed that recombinant BAR and PAT exhibit subst

223 0% of samples were undetectable using either assay, showing full concordance with the TaqMan assay.

224 hindered by issues of sample preparation and assay signal-to-noise.

225 of 69 TE subfamilies by luciferase reporter assays, spanning all major TE classes, and showed that 9

226 The assay successfully detected purified norovirus RNA from

227 1 patients using enzyme-linked immunosorbent assay (Tac/Sir = 104, Tac/Mtx = 107).

228 An RT-RPA assay targeting a recent epidemic human norovirus strain

229 more labor-intensive and time-consuming cell-assay technique, 2.0 mug/mL.

230 investigate this claim, we developed a novel assay that allows for parallel profiling of activity on

231 ntrinsic termination, we developed a kinetic assay that distinguishes effects of TL alterations on th

232 ng real-time polymerase chain reaction (PCR) assays that facilitate direct analysis of a single infec

233 High-throughput assays that produce hundreds of measurements per sample

234 Radioligand binding studies and functional assays that use human receptors expressed in Chinese ham

235 nual Blue Carba assay, the Modified Carba NP assay, the boronic acid synergy test, and the metallo-be

236 with the exception of the Manual Blue Carba assay, the Modified Carba NP assay, the boronic acid syn

237 and HIV samples tested by the two automated assays, the percentage of agreement was excellent at 99.

238 multichannel pipets enable many established assays, they do not support analytical tools with custom

239 , we used an OLINK high-throughput proteomic assay to analyze moderate-to-severe AD (n = 59) compared

240 Here we utilize a functional assay to assess the ex vivo drug sensitivity of single m

241 sublines was first subjected to a parasitism assay to determine its resistance phenotype and in a sec

242 interferon enzyme-linked immunosorbent spot assay to interrogate CD8(+) and CD4(+) T cell responses

243 irs exist, but there is currently no optimal assay to measure HIV reservoirs in HIV eradication inter

244 nd independently cross-validated a biomarker assay to predict mortality based on genes whose expressi

245 Here we describe a reporter cell-based assay to quantify inducible, replication-competent laten

246 timized a firefly luciferase complementation assay to screen against this chimeric receptor.

247 combined molecular modeling with biological assays to ascertain how modifications of phosphonocarbox

248 c applications ranging from in vitro sensing assays to cellular imaging are separated and discussed i

249 omical, electrophysiological, and behavioral assays to determine their patterns of connectivity and a

250 rmation derived from separate, complementary assays to gain higher-confidence insights into cellular

251 valuated with robust, sensitive and specific assays to quantitate reactivatable latent virus.

252 They miniaturize assays to reduce sample and reagent consumption and incr

253 Here, we set up two assays to specifically identify the pharmacological prop

254 glutaminase (anti-TG2A) using a radiobinding assay, to endomysium using an immunofluorescence assay,

255 Furthermore, low-extent saccharification assays, under different pretreatment conditions, showed

256 When assayed, urinary metanephrine and catecholamine levels w

257 hrocytes (IE); however, current phagocytosis assays use IE collected from infected individuals or fro

258 be linked to the approval of new diagnostic assays used to measure efficacy or to predict response.

259 enic activities in a microvascular sprouting assay using choroid explants.

260 ues in the GpppA formation and pRNA transfer assay using GDP analogues as pRNA acceptors indicated th

261 A URAT1 binding assay using radiolabeled verinurad revealed that distinc

262 Interestingly, transwell assays using Madin-Darby canine kidney II cell line cell

263 ng, we conducted hemagglutination inhibition assays using virions and ISVPs.

264 Competitive serial transplantation assays using Zfp521-deficient mice revealed that ZFP521

265 The emergence of real-time PCR assays utilizing allele-specific molecular detection tec

266 The new assay was able to detect RNA from five currently used va

267 A biochemical complementation assay was developed to show that a unique, conserved dom

268 A comprehensive DNA microarray-based assay was performed on all isolates to identify beta-lac

269 transferase-mediated dUTP nick-end labeling assay was performed, and intestinal inflammation severit

270 rtial thromboplastin time (aPTT) coagulation assay was performed, and the viscosity, density, and coa

271 Finally, the assay was used to detect elevated FAP activity in human

272 ly, and the total oxidizable precursor (TOP) assay was used to quantify precursors that form perfluor

273 Assay was validated by spiking OTC to antibiotic free mi

274 The assay was validated for linearity, limit of detection, s

275 Using a visual in vitro assay we previously showed that efficient protein target

276 n in vitro optical trapping and fluorescence assay, we found that Kif15 slides anti-parallel microtub

277 Using a single-copy assay, we measured HIV-1 RNA levels in CSF and plasma sp

278 the CPSF160-WDR33 subcomplex and biochemical assays, we define the molecular architecture of the core

279 Using in vivo infection assays, we demonstrate that parasites undergoing coat re

280 uorescence Job plots, and yeast three-hybrid assays, we investigate the interactions of rProtein uL23

281 ceptor-beta sequencing and tumour reactivity assays, we predict that tumour reactive T cells are freq

282 antly, through in vitro binding and activity assays, we showed that CML36 interacts directly with the

283 ting the sensitivity and selectivity of this assay were investigated, including ionic strength, AgNP

284 The identification results from the assays were 100% concordant with DNA sequencing results.

285 idues, in silico predictions, and functional assays were all informative in distinguishing the subset

286 A (spa) typing, SCCmec typing, and PCR-based assays were used to detect mecA, mecC, vanA, Panton-Vale

287 Via targeted metabolomics assays, whereby carotenoids and ascorbic acid were analy

288 quaking-induced conversion (RT-QuIC) seeding assay, which detects minute amounts of the disease-speci

289 for a universal fluorescence turn-on enzyme assay, which relies on the target enzyme-triggered DNA p

290 rone-like activity in an insulin aggregation assay, which we propose facilitates preprotein import.

291 ic devices and permit the design of cellular assays, which can ultimately impact disparities in healt

292 olox equivalents (TE)/gDM as measured by the assay with ABTS radical.

293 d Prevention standardized NA inhibition (NI) assay with oseltamivir, zanamivir, peramivir, and lanina

294 Exon-trapping assays with constructs containing splice site alleles re

295 capacity and optimized for simple screening assays with luciferase or fluorescent reporters.

296 Comparative enzyme-linked immunosorbent assays with monoclonal antibodies against PfLDH or PfIDE

297 Drug assays with patient-derived cells such as circulating tu

298 In confrontation assays with saprobic fungi that were commonly found in c

299 ubstrates were tested as prenyl acceptors in assays with three prenyltransferases, and we were able t

300 Most included assays, with the exception of the Manual Blue Carba assa