ライフサイエンスコーパス: assay system

1 expressed with wild-type L in the minigenome assay system.

2 n secretion was measured with a fluorescence assay system.

3 MO-targeted ubiquitin ligase activity in our assay system.

4 and the appropriateness of animal caps as an assay system.

5 and product are analyzed using the optimized assay system.

6 or phase, demonstrating the validity of the assay system.

7 micromolar concentration range in a cellular assay system.

8 ubstrate were added to a fluorogenic peptide assay system.

9 ct on transferrin endocytosis using the same assay system.

10 ncer-blocking capabilities using a transgene assay system.

11 DA-MB-231 breast cancer cells in a coculture assay system.

12 ic agonist, was measured with a fluorescence assay system.

13 oligosaccharides and the sensitivity of the assay system.

14 minescence output in a discontinuous coupled assay system.

15 eviously demonstrated in a purified in vitro assay system.

16 recognition complex (ORC) using an in vitro assay system.

17 17, and tumor necrosis factor-alpha using an assay system.

18 ate group from mature lipid A in an in vitro assay system.

19 e easily adapted to any bicistronic reporter assay system.

20 nd C with the TLR5 receptor using a reporter assay system.

21 ein has been studied in an in vitro adhesion assay system.

22 ation was evaluated using a defined in vitro assay system.

23 yeast (Saccharomyces cerevisiae) two-hybrid assay system.

24 ds caused some opiate activity by at least 1 assay system.

25 ble when the human plasma was present in our assay system.

26 cells in a Matrigel basement membrane matrix assay system.

27 ribution of BNP differed by age, gender, and assay system.

28 arance of spliced products using an in vitro assay system.

29 gged hENT1 in a yeast nucleoside transporter assay system.

30 seudouridylation, we established an in vitro assay system.

31 monstrated similar results using a transient assay system.

32 put screening using a P-selectin ELISA-based assay system.

33 nsequently lowers the redox potential of the assay system.

34 hen implemented within a competitive binding assay system.

35 vitro mouse embryonic stem cell (ESC) clonal assay system.

36 ith ZEB1 on the Zp ZV element in a cell-free assay system.

37 he prm240 promoter, measured in an uncoupled assay system.

38 for their reduced catalytic activity in all assay systems.

39 cation of positive controls for use in novel assay systems.

40 efficient kinase activity-dependent cellular assay systems.

41 to compare against pre-existing quantitative assay systems.

42 n APC-dependent and APC-independent in vitro assay systems.

43 nerated from these important and informative assay systems.

44 s of data obtained from bicistronic reporter assay systems.

45 ling pathway using both in vitro and in vivo assay systems.

46 mpound caused opiate assay activity in all 5 assay systems.

47 aluated in these cells using three different assay systems.

48 l lamina assembly was studied in vivo in two assay systems.

49 inhibited the survival effects of T in these assay systems.

50 uncoating, as revealed with three different assay systems.

51 s 5-HT2A and 5-HT2B in relevant tissue-based assay systems.

52 nificantly reduced enzymatic activity in all assay systems.

53 tions has been accomplished via microfluidic assay systems.

54 with specific virulent strains using several assay systems.

55 FX to attenuate FX activation in lipid-free assay systems.

56 In cell-free assay systems, 10 micro m AACOCF3 inhibited 5-lipoxygena

57 In cloned receptor assay systems, 12 displays at least 95-fold selectivity

58 To assess the accuracy and robustness of the assay system, 270 coded plasma specimens derived from ni

59 We used the beta-arrestin PathHunter assay system, a newly developed, generic GPCR assay form

60 This assay system accurately determined the level of spiked 3

61 This more physiologic assay system allowed the investigators to rigorously def

62 This assay system allows replication to be studied at extreme

63 Using a rapid in vivo assay system and a high-throughput mega-shift assay, we

64 ne were assayed in a subgenomic HCV replicon assay system and found to be potent and selective inhibi

65 are strong activators of Nrf2 in a reporter assay system and in primary human CLL based on increased

66 profile, three-dimensional (MP3D) mechanical assay system, and compared responses to those elicited b

67 The new assay system, and our findings based on it, will enable

68 emplates for data generated by dual reporter assay systems, and an online tutorial are available at o

69 nical range, diagnostic criteria, diagnostic assay systems, and treatment options for this group of d

70 DEPFMU-based assay systems are 10-100 times more sensitive for OPH an

71 is the ligand for Ly-49D in a reporter gene assay system as well as in NK cell killing assays.

72 cted for MDH or other dehydrogenases in this assay system, at physiological mole fractions of the mat

73 f reliable systems prompted us to develop an assay system based on dual enzymatic activities.

74 We describe a novel in vivo assay system based on hepatocyte transplantation that pe

75 Using an assay system based on partially differentiated embryonic

76 SpoVT we utilized an in vivo multimerization assay system based upon fusion of the domains to the DNA

77 Assay systems based on this technology were developed to

78 Using SUMO fusions, a novel assay system, based on poliovirus RNA-dependent RNA poly

79 nucleases can be preliminarily tested in our assay system before their downstream application in plan

80 In a Gal4-LUC assay system, blockade of the PI3K pathway significantly

81 only provide a novel plant pre-mRNA cleavage assay system, but also suggest a cross-kingdom functiona

82 (MAMEF), we show that a simple protein-based assay system can be optically amplified approximately 10

83 he potency and safety profiles and the CYP51 assay system can be used in structure-activity studies i

84 hich we have named EViTAS (for ex vivo tumor assay system), can recapitulate salient aspects of tumor

85 MG) assay for inorganic phosphate to form an assay system capable of determining inorganic phosphate

86 Four of the assay systems caused false-positive results for at least

87 In this assay system, changes in the emission spectrum of the fl

88 ing PCR and a semi-high-throughput, scalable assay system confirmed the initial discovery results in

89 Our previous study using an in vitro assay system demonstrated that Fgf and Gdf11 signals loc

90 Characterization of this assay system demonstrates that the FITC-labeled dextran

91 This sensitive DNA repair assay system demonstrates the complexity of response to

92 iates of DSB repair by using a budding-yeast assay system designed to mimic physiological DSB repair.

93 tomation of complex primary human cell-based assay systems designed to capture emergent properties ca

94 In the present study we used an assay system developed for poliovirus 3D(pol) to search

95 In an in vitro NHEJ assay system, DNA end joining was reduced by NF90/NF45 i

96 We developed a reporter assay system employing a self-inactivating retrovirus an

97 The assay system exhibits an intrinsic dynamic range of two

98 The reported assay system faithfully replicates the pharmacology of t

99 These findings provide a simple assay system for cardiac induction that may allow elucid

100 on, and high throughput make it an effective assay system for clinical laboratory diagnosis of HCV an

101 ned this hypothesis using a highly sensitive assay system for detection of misfolded protein seeds in

102 ia rickettsii were determined using a plaque assay system for enumeration and isolation of mutants.

103 yme donor immunoassay (CEDIA) and a Beta-Glo assay system for generation of bioluminescent signal.

104 demonstrated using a transient transfection assay system for GR transactivation.

105 h reporter provides a novel, high-throughput assay system for identifying compounds that bind to and

106 ivate 5-HT2B receptors, thus validating this assay system for its ability to predict medications that

107 nt of a 96-well plate-based purification and assay system for measuring chlorophyllase activity.

108 rudin folding is utilized here to develop an assay system for measuring the activity of disulfide oxi

109 his paper describes an in vitro fluorometric assay system for protein splicing based on the RecA inte

110 ong with gene fusion approaches, a sensitive assay system for signal sequences that utilize the SRP p

111 We previously constructed an assay system for studying enterobacterial beta-lactam re

112 We have established a quantitative assay system for the analysis of cap interaction with PB

113 Efforts to develop a standardized 2-tier assay system for the detection of antiretinal antibodies

114 This integrated assay system for the detection of HIV-1 drug resistance

115 A novel dot blot-based assay system for the detection of IgE against alpha-Gal

116 r the zebrafish pronephros can be used as an assay system for the development of glomerular function

117 As such, this will be a valuable assay system for the dissection of MITE transposition an

118 ur characterization of an in vitro signaling assay system for the mechanism of Tax-mediated activatio

119 To establish a simple and rapid assay system for the monitoring of R5 HIV-1 replication

120 , we describe the development of an in vitro assay system for the rapid detection and quantification

121 its meiotic spindle will provide a sensitive assay system for the study of reproductive toxins.

122 method should provide a fluorescent reporter assay system for the wide variety of beta-gal systems cu

123 une response, and the sensitivity of various assay systems for detecting WNV in blood.

124 sults from phenotypic screenings in cellular assay systems for viral replication.

125 newly developed mitochondrial protein import assay system from Trypanosoma brucei to demonstrate that

126 In a human whole-blood assay system, GBS type III COH-1 potently induced substa

127 PSMA7 RNA inhibited HCV IRES activity in two assay systems: GCV resistance in the HeLa IRES TK report

128 Finally, in our assay systems, H(2)O(2)-induced cell death was not due t

129 light, a sensitive antibody (Ab) microarray assay system has been developed for specific detection o

130 This assay system has the potential for simultaneous screenin

131 Bicistronic reporter assay systems have become a mainstay of molecular biolog

132 Two homogeneous assay systems have been combined to provide a new cell-b

133 We now show that, using the DASL assay system, highly reproducible tissue- and cancer-spe

134 oblasts to pluripotency, indicating that the assay system identifies functional Oct4 cofactors.

135 used the Drosophila ventral epidermis as our assay system in a series of genetic screens to identify

136 undertaken to determine the accuracy of this assay system in detecting resistance-associated mutation

137 imeras and Gal4-Luc reporter co-transfection assay system in HepG2 cells.

138 previously determined using a tissue culture assay system in human cells (HeLa).

139 abidopsis plant as well as the SEAP reporter assay system in MCF-7 breast cancer cell-line; the anti-

140 The lack of such an assay system in plants hampered the study of plant mRNA

141 We have developed an assay system in Saccharomyces cerevisiae to study recipr

142 After evaluating the assay system in the hybrid paper-SPCE cell, the three-el

143 as evaluated utilising the pER8:GUS reporter assay system in transgenic Arabidopsis plant as well as

144 was confirmed in a cell-based mineralization assay system in vitro.

145 We used an assay system in which hundreds of meiotic spindles can b

146 initially evaluated using an in vitro shear assay system in which interactions between circulating c

147 r fluorescence recovery after photobleaching assay system in which two H1 proteins fused to spectrall

148 on using different transduced cell lines and assay systems in the absence of an independent thymidine

149 and characterized a plant in vitro cleavage assay system, in which nuclear protein extracts from Ara

150 This assay system includes a semiautomated procedure, using 9

151 his paper describes a microfabricated enzyme assay system including a micromixer that can be used to

152 in a variety of cell-free and in vitro redox assay systems, including oxidant production by transitio

153 This assay system is able to detect enzymatic activity at enz

154 data analysis, and the modular design of the assay system is amenable to straightforward adaptation f

155 This assay system is capable of detecting and differentiating

156 The assay system is comprised of a plastic microfluidic cart

157 ection, consensus agreed that a standardized assay system is needed to detect serum antiretinal antib

158 A major merit of the proposed assay system is the potential to accommodate the analysi

159 We believe that this assay system is uniquely capable of advancing our unders

160 termed the multiple-enzyme/multiple-reagent assay system (MEMRAS), relies on the use of reagents suc

161 ed, as a read-out, a single kind of cellular assay system: neuroblastoma cells that are persistently

162 We have developed an assay system of budded supported membrane tubes displayi

163 se DNA contamination was detected in the PCR assay system or the clinical samples.

164 In a reporter assay system, ORF30 and ORF34 were required for MHV-68 t

165 members of the RsbR coantagonist family and assayed system output using a reporter fusion.

166 In an in vitro biofilm assay system, P. aeruginosa displayed strain-specific bi

167 This assay/system performed well in clinical testing of regul

168 e development of more reliable and sensitive assay systems, possibly formatted for cytochemical appli

169 Difficulties with previous assay systems prompted us to develop retroviral vectors

170 The combined purification and assay system provides a convenient and rapid method for

171 this SNP linkage panel with the multiplexed assay system provides a previously unattainable level of

172 SUMOylation in both whole-cell and cell-free assay systems, raising the possibility that the deacetyl

173 We show that our in vitro assay system recapitulates the effect of PS1 mutations o

174 target to pursue for further high throughput assay system screening.

175 We have designed a quantitative assay system sensitive enough to detect differences betw

176 This assay system should be an important tool for investigati

177 The DASL assay system should prove useful for high-throughput exp

178 und 1 and 2 was tested in different in vitro assay systems such as free radical scavenging assay, 3-(

179 tration of its substrates in a mixed micelle assay system, suggesting that catalysis occurs at the me

180 t this phenomenon occurs in a membrane-based assay system suggests that these compounds increase Abet

181 ductase and PKC zeta, transphosphorylated in assay systems supportive of activity of only one of the

182 Two different assay systems, surface plasmon resonance and a microtite

183 That assay system, termed the STOPR protocol, allowed effects

184 Here, we have developed an integrated assay system that allows simultaneous examination of dou

185 Therefore, we were looking for an assay system that can be used for heme concentration det

186 dy was to develop an alternative, label-free assay system that can reliably measure NEM and PSA in pa

187 nt of a high-throughput circadian functional assay system that consists of luminescent reporter cells

188 cal end points in dynamic networks and as an assay system that could be used to identify electric phe

189 ped a Ni(2+)-nitrilotriacetic acid liposomal assay system that efficiently conjugates histidine-tagge

190 scribe an Escherichia coli-based integration assay system that has allowed us to characterize attN1 i

191 therefore developed a low-phosphate, low-pH assay system that is more realistic with respect to soil

192 alpha activity, determined using an in vitro assay system that lacked lipids, were found to be a nonl

193 c phenotype were characterized using a novel assay system that measures fibroblast behavior in respon

194 This protocol describes an assay system that permits examination of synaptogenic ac

195 his report, we used a sensitive derepression assay system that we developed previously to reexamine t

196 s to be screened, it is important to develop assay systems that are not only sensitive but also homog

197 In the search for such molecules, assay systems that can be adapted to high-throughput scr

198 eloped a suite of whole-blood, syringe-based assay systems that can be used to reproducibly assess in

199 relied on cell-free or accessory cell-based assay systems that do not accurately reflect in vivo res

200 d we demonstrate by the use of two different assay systems that Kap114p inhibits Nap1p-mediated chrom

201 ted, partly due to lack of reliable in vitro assay systems that reflect the in vivo functionality of

202 ves associated with frequently used NAD/NADH assay systems that rely on detection of NADH using UV ab

203 th radiolabeled N-blocked aminoacyl-tRNAs in assay systems that require the separation of radiolabele

204 We also showed using a transient expression assay system, that the dls1 mutation results in a decrea

205 Using this assay system the dislocation of MHCI heavy chains was fo

206 Using retinyl palmitate in a micellar assay system the enzyme was active over a broad pH range

207 In a mixed micelle assay system, the apparent Km for the precursor lipid IV

208 ve developed a widely applicable recruitment assay system through which we find that BAF opposes PRC

209 We designed an assay system to allow titration of the redox state of th

210 This study established a robust assay system to analyze the function of BRCA1 in regulat

211 In this study, we developed a binding assay system to characterize EAAT ligands for all EAAT s

212 n the present study, we used the Gal4 fusion assay system to characterize the mechanism of transactiv

213 We have established a fully defined in vitro assay system to delineate mechanisms for Pol II transit

214 Furthermore, we established an assay system to detect ISGylated target proteins by cotr

215 tro and cellular activity, a high-throughput assay system to detect the attachment of [(3)H]geranylge

216 llifera (AmTRP-R) in a heterologous reporter assay system to determine the activities of various liga

217 feasibility of using the cell line CAD as an assay system to dissect the signaling pathways triggered

218 They use this cell-based assay system to help explain the clinical manifestations

219 Previously, we have used an assay system to identify astrotactin as a neuronal ligan

220 Previously, we utilized a unique assay system to identify mutations that increased minisa

221 The lens provides an assay system to identify pathways critical for fiber cel

222 eloped malachite green-based high throughput assay system to identify SerB2 inhibitors.

223 , we exploit a sensitized human primary cell assay system to investigate the biological function of d

224 Here we report an in vitro assay system to measure synthesis of both mtDNA and 7S D

225 ssays were developed on a robust multiplexed assay system to provide a combination of very high accur

226 activity were investigated using an in vitro assay system to provide further insight into the mechani

227 nd demonstrate the feasibility of using this assay system to rapidly screen for compounds that modula

228 ier report, we optimized a microtiter format assay system to screen potential bioactive compounds usi

229 igase was used in a ligation-based detection assay system to show increased sensitivity over T4 DNA l

230 Second, we employ this assay system to show that a dualsteric design principle,

231 We used a solid-phase kinase assay system to show that PKR does not transfer its own

232 st, we used a human PBMC-based proliferation assay system to simultaneously monitor the effect of TLR

233 th high resolution should provide a valuable assay system to study and characterize various types of

234 The availability of a transplantation assay system to unequivocally identify male germline ste

235 We used cell-based assay systems to investigate the mechanisms responsible

236 t BMP signaling, we developed novel in vitro assay systems to monitor trans signaling using Drosophil

237 We use two assay systems to show that cellular PTEN phosphatase act

238 gainst F(1) in a 96-well microplate activity assay system, to establish a method for fast high throug

239 ents derived from cells and other biological assay systems treated with small molecules.

240 We have established a transient-transfection assay system under which the activity of various NR is h

241 eliable cell-based high-throughput screening assay system using an HCV replicon model to identify sma

242 /activin subfamily, we developed a cell-free assay system using soluble receptor-Fc fusion proteins.

243 We have developed a novel assay system using Streptolysin-O permeabilized neutroph

244 We developed a live-cell high-throughput assay system using the baker's yeast Saccharomyces cerev

245 rthermore we report a simple "membrane-free" assay system using the V0 proton channel-specific inhibi

246 Assay systems using the fluorogenic aldehyde were valida

247 We developed two assay systems (using human dendritic cells and Aedes aeg

248 The assay systems utilize three proprietary technologies: (i

249 vein endothelial cells (HUVEC) with a novel assay system utilizing HUVEC immobilized on microcarrier

250 Importantly, our cuvette-based SPR assay system was also suitable for the monitoring of lig

251 This assay system was designed to specifically detect framesh

252 In this study, a novel assay system was developed to compare the catalytic effe

253 A refined assay system was developed to establish that Saccharomyc

254 Ts to HIV mutagenesis, a new high-throughput assay system was developed.

255 The assay system was employed to measure the time dependence

256 The reported assay system was exclusively formed by nucleic acid olig

257 The limit of detection (LOD) of CT in the assay system was found to be 10 fg/mL which is equivalen

258 The assay system was found to be amenable to sensitive kinet

259 The Dual Glo Luciferase Assay System was used to determine reporter activities.

260 This assay system was used to investigate the kinetics of tra

261 This assay system was used to screen for embryo-defective mut

262 A model FRET-based caspase-3 assay system was used to test the performance of the que

263 The assay system was validated by screening potential cataly

264 However, using an in vitro assay system we demonstrate that H. pylori WaaA is a bif

265 As an application of this assay system, we characterized the association of transc

266 beta(3) subunits, in a transient expression assay system, we compared the efficiencies of infection

267 With this assay system, we demonstrate that the replication capaci

268 Using transfected cells as an in vitro assay system, we demonstrate that truncated syntaxins la

269 By using an in vitro assay system, we demonstrate the presence of lipid A 1-p

270 Previously, using a chromosomal reversion assay system, we established that an adaptive mutagenic

271 Utilizing this assay system, we find that gene conversion events at the

272 Using an in vitro assay system, we found that expression of wild type huma

273 Surprisingly, using a recently developed assay system, we found that these factors are not direct

274 RXR-VDR mammalian two-hybrid (M2H) biologic assay system, we measured vitamin D metabolite uptake an

275 on ubiquitination of p27 in a reconstitution assay system, we now report a noncatalytic requirement f

276 ng this three-channel terbium-based, TR-FRET assay system, we show in one experiment that the additio

277 Using a transformation-based assay system, we show that most of the apparent BIR even

278 h mass spectrometry analysis and an in vitro assay system, we showed that the loss of LpxEHP activity

279 ing a tetracycline-regulated gamma-GCSh mRNA assay system, we systematically dissected the cis-acting

280 Using different assay systems, we present evidence that ADAMTS13 binds t

281 sing immunoblot-based as well as single-cell assay systems, we present evidence that Drosophila glypi

282 Using chicken embryonic endoderm as an assaying system, we found that ectopic Mfng expression i

283 As such, several in vivo assay systems were developed to evaluate gene editing ca

284 ations were defined in pilot rat studies and assay systems were used with xanthine oxidase and activa

285 umi-Gal 530), and a bioluminescent (Beta-Glo Assay System), were employed in the study.

286 ture of Cyt b was obtained using a cell-free assay system where p47SH3AB enhanced superoxide producti

287 In this work, we developed a novel BiLC assay system which is sensitive and specific to quantify

288 To this end, we developed a new assay system which supports very high degrees of multipl

289 llectively, these findings suggest that this assay system, which we have named EViTAS (for ex vivo tu

290 However, assay systems, which enable the straightforward discover

291 esis, two separate three-dimensional culture assay systems, which model chick atrioventricular cushio

292 Using this assay system, wild-type collagen was compared to a uniqu

293 Our cell-free binding assay system will allow for testing of models of recepto

294 It is anticipated that this high-throughput assay system will greatly facilitate nucleotidyltransfer

295 oduction of ROS was measured using a coupled assay system with Amplex Red.

296 Utilizing a model sandwich assay system with biotinylated anti-IgG as the capture a

297 Our results suggest that this novel assay system with this new TDP2 substrate can be used fo

298 In addition, we have developed an in vitro assay system with which we demonstrate that purified, ac

299 ated potent enzyme activity in a chromogenic assay system, with select examples also demonstrating po

300 mutants in the crystal violet-based biofilm assay system yielded six mutants that exhibited greatly