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1 ules by the sperm-derived centrosomes (sperm asters).
2 onucleus is independent of the sperm and its aster.
3 rotubules early in the assembly of the sperm aster.
4 t not with microtubule remnants of the sperm aster.
5 ear attachment and migration along the sperm aster.
6  capture of the meiotic spindle by the sperm aster.
7 m a mature centrosome that nucleates a sperm aster.
8 nsitions from actin vortices over stars into asters.
9 tes boundaries to microtubule growth between asters.
10 icrotubules to centrosome-associated mitotic asters.
11 f-assembly of structures such as microtubule asters.
12 ments emanate from the plasmid DNA in radial asters.
13 a recombinant 4.1R reconstituted the mitotic asters.
14 nt manner in the direction of the separating asters.
15 nd induced rapid disassembly of preassembled asters.
16  in bipolar spindles associated with ectopic asters.
17 o a polarizing cue associated with the sperm asters.
18 d disassembly of bipolar spindles into large asters.
19 pindle poles, mimics Ran's ability to induce asters.
20 s from microtubules emanating from the sperm asters.
21  (ch-TOGp) is an abundant component of these asters.
22 , centrosome maturation and the formation of asters.
23 umulate large amounts of DNA and microtubule asters.
24 bers to study the positioning of microtubule asters.
25  that furrows always assemble midway between asters.
26  molecular motors, is sufficient to position asters.
27 ad to a strong anisotropy of the microtubule asters.
28 he spindle poles toward the centers of these asters.
29 ects, which lead to a weak anisotropy of the asters.
30  bundle to form a bipolar spindle that lacks asters.
31 linked apolar asters, and a lattice of polar asters.
32 s as a proxy for the movement of microtubule asters.
33 vement of yolk granules toward the center of asters.
34 at reduced levels at cortical sites near the asters.
35 rest and decreased formation of mitotic-like asters.
36 ulation, we produced "cells" containing only asters, a truncated central spindle lacking both asters
37                            These microtubule asters accurately reflect the noncentrosomal aspects of
38              Conversely, in anucleate cells, asters alone can support furrow induction without a spin
39 ng fertilization by nucleating a microtubule aster along which the female pronucleus migrates toward
40 as used to measure the diameter of the sperm aster and assign a score (0-3) based on the degree of ra
41 ependent difference in diameter of the sperm aster and in the organization of the sperm astral microt
42  and the more mechanism-based assignments of ASTER and MOAtox.
43 upporting observations, including testing of aster and ring function with inhibitors.
44 ization in extracts, dramatically inhibiting aster and spindle assembly and also depolymerizing prefo
45        Stable associations between the sperm aster and the pronuclei are essential during this direct
46 mulate to high levels at sites distal to the asters and at reduced levels at cortical sites near the
47 buttercups [Ranunculaceae pro parte (p.p.)], asters and campanulas (Asterales), bluets (Rubiaceae p.p
48 rs, a truncated central spindle lacking both asters and chromosomes, or microtubules alone.
49 nctional interaction between the microtubule asters and cortical actin has been largely analyzed in a
50 ng" that enforces radial organization within asters and generates boundaries to microtubule growth be
51 lso observed cells with multiple cytoplasmic asters and MTs lacking an organizing center.
52  blocked the interpenetration of neighboring asters and recruited cytokinesis midzone proteins, inclu
53 anL43E) induced the formation of microtubule asters and spindle assembly, in the absence of sperm nuc
54                 Signaling by the centrosomal asters and spindle midzone coordinately directs formatio
55  extracts results in compromised microtubule asters and spindles and the mislocalization of XMAP215,
56 atase Ran stimulates assembly of microtubule asters and spindles in mitotic Xenopus egg extracts.
57 nts into dynamic patterns, such as vortices, asters and stars.
58 xtracts depleted of Ndel1 are unable to form asters and that this defect can be rescued by the additi
59 ns of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mit
60  the Verhaar scheme, 1165 were classified by ASTER, and 802 were available in MOAtox.
61 ion, namely bundled filaments, linked apolar asters, and a lattice of polar asters.
62 to interpolar MT bundles, half spindles, and asters, and is enriched around spindle poles.
63 ilization, optimal starting distance between asters, and proximity to chromatin all favored CPC recru
64  (MTs) of the first mitotic spindle, spindle asters, and the cortical MTs, but not with microtubule r
65 ive MAP kinase is localized at kinetochores, asters, and the midbody during mitosis.
66  expressed as transient assembly of cortical asters, and this cortical reorganization was altered in
67  two other microtubule structures: the sperm aster; and the radial, monastral array of microtubules e
68                                 We find that aster anisotropy is biased in the direction of the chrom
69                    In both, large numbers of asters appeared at the cortex of the egg after completio
70 on, lateral interactions between microtubule asters are assumed to be important for regular spatial o
71                                        These asters are foci of microtubules, motors, and microtubule
72 s, mitochondria in the region of the spindle asters are labeled.
73         Our results also indicate that sperm asters are not essential for pronuclear migration but ar
74 ts, showing that microtubule linkages within asters are remarkably compliant (mean stiffness 0.025 pN
75 irst, the aggregation of microtubule foci or asters around the chromosomes, and second, the elongatio
76 ng and were specific for mitotic centrosomal asters as we observed little effect on interphase asters
77 lymerization of microtubules was measured in aster assays suggesting a role for MAP kinase in regulat
78 ved little effect on interphase asters or on asters assembled by the Ran-mediated centrosome-independ
79 h-TOGp is a major constituent of microtubule asters assembled in a mammalian mitotic extract and that
80 les in the microtubule pellet of the mitotic asters assembled in mammalian cell-free mitotic extract.
81                                  Microtubule asters assembled in response to centrosomes and Ran-GTP
82 e microtubule-associated proteins in mitotic asters assembled in vitro.
83 n mitotic extracts and reconstitutes mitotic aster assemblies in 4.1R-immunodepleted extracts in vitr
84 n vitro using a cell-free system for mitotic aster assembly and in vivo after injection into cultured
85 nization in murine oocytes and taxol-induced aster assembly in cultured cells.
86 he mitotic spindles, and its role in mitotic aster assembly in vitro.
87 he spindle poles and for mitotic microtubule aster assembly in vitro.
88 importin-beta is an inhibitor of microtubule aster assembly in Xenopus egg extracts and that Ran regu
89 oteins from the cell free system for mitotic aster assembly indicates that the plus end-directed acti
90            XNercc immunodepletion also slows aster assembly induced by Ran-GTP, producing Ran-asters
91 uRCs from centrosomes, inhibited microtubule aster assembly, and induced rapid disassembly of preasse
92 pADPr, extended from PARP-5a, also triggered aster assembly, suggesting a functional role of the pADP
93 ogical MT dynamics as assayed by Ran-induced aster assembly.
94 ion that ch-TOGp is required for microtubule aster assembly.
95  and dynactin to microtubules during spindle/aster assembly.
96 f M9M to mitotic cytosol induces microtubule aster assembly.
97                      None of the cytoplasmic asters associated with the zygotic nucleus and, as in un
98 function prevents the development of mitotic aster asymmetry and spindle pole movement towards the su
99  a key determinant in organizing microtubule aster asymmetry to power nuclear dynein-dependent separa
100 g the first phase (establishment), the sperm asters at one end of the embryo exclude the PAR-3/PAR-6/
101                                 Simultaneous aster attenuation and MP-GAP inhibition led to RhoA accu
102 ins after pronuclear meeting, when the sperm asters begin to invade the anterior.
103  broadened by attenuation of the centrosomal asters but was not affected by MP-GAP inhibition alone.
104     Here, we explore the contribution of the asters by analyzing the consequences of altering interas
105                         We show that dynamic asters can also be obtained from a homogeneous solution
106 were released and translocated away from the aster center.
107                                   Interphase asters center and orient centrosomes with dynein-mediate
108             Rather, the preceding interphase aster centers and orients a pair of centrosomes prior to
109 lation is important to focus microtubules at aster centers and to facilitate the transition from aste
110  centrifugal clearing of F-actin from around aster centers.
111                         We conclude that the asters confer accuracy and precision to a primary furrow
112 tubule (MT)-binding proteins, Orbit/multiple asters/cytoplasmic linker protein-associated protein, ha
113 gnaling-based cortical forces pulling on the asters, delays furrow formation and leads to the formati
114        The organization of microtubules into asters depends on 4.1R in that immunodepletion of 4.1R f
115 ion from bull A resulted in an average sperm aster diameter of 101.4 microm (76.3% of oocyte diameter
116 ers (P < or = 0.0001) from the average sperm aster diameters produced after inseminations from bull B
117 anillin (ANI-1) promotes the formation of an aster-directed furrow in Caenorhabditis elegans embryos.
118                                           In aster-directed furrowing, cytoskeletal factors accumulat
119 ation is reasonably well understood, but the aster-directed pathway is not.
120                                          The aster disassembled during anaphase, leaving the spindle
121 tile ring formation, with anaphase entry and aster disassembly also required for polar body biogenesi
122 onal alignment, we find that monopolar sperm asters do not show evidence for flux, partially contradi
123 and cell cycle kinase Aurora A along spindle asters during cell division.
124 (KIF2C) also resulted in ectopic microtubule asters during mitosis in C. elegans zygotes or HeLa cell
125 , we propose that signaling by the separated asters executes two critical functions: 1) it couples fu
126                      Our model predicts that asters expand as traveling waves and recapitulates all m
127 We also show that (acentrosomal) microtubule asters fail to assemble in vitro without HSET activity,
128 In mutant embryos arrested in meiosis, sperm asters fail to form, and posterior is defined by the pos
129 , in the absence of cohesin, mitotic spindle asters failed to form in vitro.
130 rom Xenopus laevis eggs to study microtubule aster formation and microtubule dynamics in the presence
131                            Using an in vitro aster formation assay, we found that BRCA1-dependent ubi
132 he NuMA tail was shown to induce microtubule aster formation by mediating microtubule bundling.
133 active as a ubiquitin ligase did not inhibit aster formation by the centrosome.
134  by specific antibodies impaired microtubule aster formation from purified mitotic centrosomes in vit
135 pletion from egg extracts delays microtubule aster formation from sperm basal bodies.
136 mation by blocking or reducing the degree of aster formation in chosen regions of the sample, without
137        Rev has a strong inhibitory effect on aster formation in Xenopus egg extracts, demonstrating t
138                                   Centrosome aster formation is reconstituted when these inactive, sa
139 increasing LIS1 concentration partly rescues aster formation, suggesting that Ndel1 is a recruitment
140 ized, tangle-shaped microtubules and reduced aster formation, which however did not alter appreciably
141 imbalances in motor force during microtubule aster formation.
142 crotubules that is capable of self-organized aster formation.
143  in centrosome amplification and microtubule aster formation.
144 any cellular structures, such as the dynamic asters found in mitotic and meiotic spindles.
145 f an organized centrosome and its associated aster from one of the spindle poles, whereas the centros
146                     Microtubules grew out as asters from artificial centrosomes and met to organize a
147 C recruitment to microtubule bundles between asters from the same spindle.
148 rmal velocities, but reduced the ejection of asters from the spindles, blocked chromosome decondensat
149  a cell-free system derived from eggs, where asters grew to hundreds of microns in diameter.
150  growth is initiated by centrosomes but that asters grow by propagating a wave of microtubule nucleat
151                                      ParM-R1 asters grow from centrosome-like structures consisting o
152                  It has been unclear whether asters grow to fill the enormous egg by the same mechani
153 were also required for radial order of large asters growing in isolation, apparently to compensate fo
154                        The standard model of aster growth assumes a fixed number of microtubules orig
155                              We propose that aster growth is initiated by centrosomes but that asters
156 n dynamics to develop a biophysical model of aster growth.
157 waves and recapitulates all major aspects of aster growth.
158 utward, but this was not essential for rapid aster growth.
159 ch aster pairs from the same spindle (sister asters) have chromatin between them, whereas asters pair
160                       NDVI was obtained from ASTER images.
161 icrotubules that either constitute the sperm aster in in vitro-fertilized (IVF) oocytes or originate
162 l F-actin ring that closely approximated the aster in location, measured diameter range, and pattern.
163 actile ring was determined by the peripheral aster in Spisula.
164 e addressed this question by imaging growing asters in a cell-free system derived from eggs, where as
165 rowing ends) of the filaments of microtubule asters in a KB cell extract.
166  to the mechanical properties of microtubule asters in a manner consistent with its localization to s
167 re, analysis of partially reconstituted MTOC asters in cells that escape complete repolymerization bl
168 e contributions of RhoA flux and centrosomal asters in controlling RhoA zone dimensions.
169 an-GTP caused normal assembly of microtubule asters in depleted extracts, indicating that this defect
170 otubule self-organization using Ran-mediated asters in meiotic Xenopus egg extracts.
171 d motor-dependent self-organized microtubule asters in metaphase-arrested Xenopus egg extracts.
172 n interaction zone that forms between sister-asters in telophase.
173 dles or the generation of single microtubule asters in the droplets.
174 petent to nucleate microtubules and assemble asters in the same cytoplasm.
175 ubules have previously been observed to form asters in vitro.
176 arallel bundles at interaction zones between asters in Xenopus egg extracts.
177 zation of Taxol-stabilized microtubules into asters in Xenopus meiotic extracts revealed motor-depend
178 ganized and centrosome-nucleated microtubule asters indicates that 4.1 is involved in regulating both
179 otofilament bundles emanating from different asters interconnect, mimicking the closure of the FtsZ r
180  monastral arrays of microtubules, the sperm aster is reduced in size, and the centrosomes often diss
181 ccurs when a small, late-growing microtubule aster is visible at the centrosome.
182                                 Although the aster labeling was constant from the time of nuclear env
183 en mating cells come into contact, they form aster-like actin structures that direct cell wall remode
184 in a mammalian mitotic extract organize into aster-like arrays in a centrosome-independent process th
185 resulted in the contraction of the gels into aster-like arrays.
186 ion of purified tubulin and the formation of aster-like microtubule structures.
187 Initially, FtsZ nucleation centers grow into aster-like structures that dramatically resemble microtu
188                                  Where these asters meet at the midplane, they assemble a disk-shaped
189 titution in cell-free extracts permits sperm aster microtubule assembly in vitro.
190  nuclear membrane available to interact with aster microtubules.
191                    In unfertilized eggs, the asters migrated inwards and two of them became stably as
192 ry revealed an important role of microtubule aster migration through cytoplasmic space, which depende
193 ve evolution, and we advocate for the use of aster modeling as a rigorous basis for achieving this go
194                                     However, aster morphology in this model does not scale with cell
195                     Our results suggest that asters observed in large fish and amphibian eggs are a m
196  Fus1, actin, and type V myosins revealed an aster of actin filaments whose barbed ends are focalized
197 ized in the centriolar adjunct from which an aster of microtubules emanates.
198 r assembly induced by Ran-GTP, producing Ran-asters of abnormal size and morphology.
199 m a cell free system for assembly of mitotic asters or antibody microinjection into cultured cells le
200  organized into distinct structures, such as asters or bundles.
201 d in defective spindle structures resembling asters or half-spindles.
202 s as we observed little effect on interphase asters or on asters assembled by the Ran-mediated centro
203 ch noncentrosomal protein during microtubule aster organization and suggests that microtubule organiz
204 otor (NuMA) proteins involved in microtubule aster organization.
205 f forces acting on microtubules and restores aster organization.
206                                        Sperm asters organized by bull A-derived sperm had an average
207 icrotubule plus-ends pushing the microtubule aster outward and that the balance of these forces posit
208 ation provides a natural experiment in which aster pairs from the same spindle (sister asters) have c
209                        In frogs, only sister aster pairs induce furrows.
210 asters) have chromatin between them, whereas asters pairs from different spindles (nonsisters) do not
211                During formation of the polar aster phase, advection of the self-organizing actomyosin
212 bly or taxol stabilization of the peripheral aster produced poorly defined rings or bulging anaphase
213 ation in vitro and in vivo and sequesters an aster promoting activity (APA) that consists of multiple
214 rm pronucleus and its associated centrosomal asters provide a cue that establishes the anterior-poste
215 that the large number of microtubules in the asters provides a highly precise mechanism for positioni
216 y (EPA) ASsessment Tool for Evaluating Risk (ASTER) QSAR (quantitative structure activity relationshi
217                                  Microtubule asters - radial arrays of microtubules organized by cent
218 ale pronucleus and the remaining cytoplasmic asters rapidly disappeared.
219                    We found that only sister asters recruited two conserved furrow-inducing signaling
220 y and promote the formation of interphase MT asters required for normal nuclear spacing, centrosome s
221 ich beautiful structures form resembling the asters seen in cell division.
222 investigate the mechanism that keeps the two asters separate and forms a distinct boundary between th
223                      Simultaneously delaying aster separation and disrupting midzone-based signaling
224                                   Preventing aster separation, by simultaneously inhibiting TPXL-1 an
225                                     Delaying aster separation, by using TPXL-1 depletion to shorten t
226                                              Aster shape is determined by interactions of the expandi
227 s study describes a paternal effect on sperm aster size and microtubule organization during bovine fe
228  "early" step, manifested by greatly reduced aster size during early time points in maskin-depleted e
229  data support the hypothesis that peripheral aster spreading, perhaps dynein-driven, is causally rela
230 o quantify bundling in the whole microtubule aster structure and a way to compare the simulated resul
231  Retriever for Successful Revascularization (ASTER) study was a randomized, open-label, blinded end-p
232 phorylated APC/C associates with microtubule asters, suggesting that phosphatases are important.
233 uration during meiosis and growth of a sperm aster that could capture the oocyte meiotic spindle.
234 al model of the dynamic formin-filamin-actin asters that can self-organize into a contractile actomyo
235 links we applied optical trapping to mitotic asters that form in mammalian mitotic extracts.
236 gotes is positioned by two large microtubule asters that grow out from the poles of the first mitotic
237 ntation to the cell cortex using microtubule asters that grow out from the spindle poles during anaph
238 scribing spontaneous bipolarization of sperm asters that was missed previously.
239 ce of all structural constituents, including asters, the central spindle, and chromosomes.
240                                    The sperm aster then captures the female pronucleus to join the ma
241 enters and to facilitate the transition from asters to bipolar spindles.
242 most feasible was found to be binding of the asters to cytoskeletal filaments and directed transport
243  modifies the landscape over time and allows asters to explore otherwise inaccessible configurations.
244  preventing the migration of the microtubule asters to opposite sides of chromosomes.
245  into a directional migration of centrosomal asters toward chromatin and their steady-state repositio
246 could not be overcome by manipulation of the asters toward the cortex.
247 aying and attenuating the formation of sperm asters until after the period of reorganization, suggest
248 wing asters with a discontinuous jump of the aster velocity to a nonzero value.
249 e degree of radial organization of the sperm aster was also bull-dependent.
250                     The metaphase peripheral aster was confirmed to spread cortically in an umbrella-
251 equired to organize acentrosomal microtubule asters, we show that addition of either active or kinase
252 d with NuMA and XMAP215 at the center of Ran asters where its activity is regulated by Aurora A-depen
253  is a radial array of microtubules called an aster, which is nucleated by a central organizing center
254    Like smaller cells, they are organized by asters, which grow, interact, and move to precisely posi
255 a coli, can also self-organize in vitro into asters, which resemble astral microtubules.
256 rsists and organizes an abnormal microtubule aster, while iMTOCs and satellites are greatly reduced.
257 losive transition from stationary to growing asters with a discontinuous jump of the aster velocity t
258                                              Asters with short microtubules move toward the position
259 ced poorly defined rings or bulging anaphase asters within the ring center, respectively, inhibiting
260 tifying a double infection with SbGP/MPV and aster yellows (16SrI) phytoplasma.
261  and colleagues report that one PMU from the aster yellows phytoplasma strain Witches' Broom (AY-WB)
262 e 706,569-bp chromosome and four plasmids of aster yellows phytoplasma strain witches' broom (AY-WB)
263 all (+/- 10 kDa) virulence effector SAP11 of Aster Yellows phytoplasma strain Witches' Broom (AY-WB)
264 plete repeat among the PMUs in the genome of Aster Yellows phytoplasma strain Witches' Broom (AY-WB).
265 rabidopsis thaliana) expressing the secreted Aster Yellows phytoplasma strain Witches' Broom protein1
266 starvation responses, we found that secreted Aster Yellows phytoplasma strain Witches' Broom protein1

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