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1 ules by the sperm-derived centrosomes (sperm asters).
2 onucleus is independent of the sperm and its aster.
3 rotubules early in the assembly of the sperm aster.
4 t not with microtubule remnants of the sperm aster.
5 ear attachment and migration along the sperm aster.
6 capture of the meiotic spindle by the sperm aster.
7 m a mature centrosome that nucleates a sperm aster.
8 nsitions from actin vortices over stars into asters.
9 tes boundaries to microtubule growth between asters.
10 icrotubules to centrosome-associated mitotic asters.
11 f-assembly of structures such as microtubule asters.
12 ments emanate from the plasmid DNA in radial asters.
13 a recombinant 4.1R reconstituted the mitotic asters.
14 nt manner in the direction of the separating asters.
15 nd induced rapid disassembly of preassembled asters.
16 in bipolar spindles associated with ectopic asters.
17 o a polarizing cue associated with the sperm asters.
18 d disassembly of bipolar spindles into large asters.
19 pindle poles, mimics Ran's ability to induce asters.
20 s from microtubules emanating from the sperm asters.
21 (ch-TOGp) is an abundant component of these asters.
22 , centrosome maturation and the formation of asters.
23 umulate large amounts of DNA and microtubule asters.
24 bers to study the positioning of microtubule asters.
25 that furrows always assemble midway between asters.
26 molecular motors, is sufficient to position asters.
27 ad to a strong anisotropy of the microtubule asters.
28 he spindle poles toward the centers of these asters.
29 ects, which lead to a weak anisotropy of the asters.
30 bundle to form a bipolar spindle that lacks asters.
31 linked apolar asters, and a lattice of polar asters.
32 s as a proxy for the movement of microtubule asters.
33 vement of yolk granules toward the center of asters.
34 at reduced levels at cortical sites near the asters.
35 rest and decreased formation of mitotic-like asters.
36 ulation, we produced "cells" containing only asters, a truncated central spindle lacking both asters
39 ng fertilization by nucleating a microtubule aster along which the female pronucleus migrates toward
40 as used to measure the diameter of the sperm aster and assign a score (0-3) based on the degree of ra
41 ependent difference in diameter of the sperm aster and in the organization of the sperm astral microt
44 ization in extracts, dramatically inhibiting aster and spindle assembly and also depolymerizing prefo
46 mulate to high levels at sites distal to the asters and at reduced levels at cortical sites near the
47 buttercups [Ranunculaceae pro parte (p.p.)], asters and campanulas (Asterales), bluets (Rubiaceae p.p
49 nctional interaction between the microtubule asters and cortical actin has been largely analyzed in a
50 ng" that enforces radial organization within asters and generates boundaries to microtubule growth be
52 blocked the interpenetration of neighboring asters and recruited cytokinesis midzone proteins, inclu
53 anL43E) induced the formation of microtubule asters and spindle assembly, in the absence of sperm nuc
55 extracts results in compromised microtubule asters and spindles and the mislocalization of XMAP215,
56 atase Ran stimulates assembly of microtubule asters and spindles in mitotic Xenopus egg extracts.
58 xtracts depleted of Ndel1 are unable to form asters and that this defect can be rescued by the additi
59 ns of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mit
63 ilization, optimal starting distance between asters, and proximity to chromatin all favored CPC recru
64 (MTs) of the first mitotic spindle, spindle asters, and the cortical MTs, but not with microtubule r
66 expressed as transient assembly of cortical asters, and this cortical reorganization was altered in
67 two other microtubule structures: the sperm aster; and the radial, monastral array of microtubules e
70 on, lateral interactions between microtubule asters are assumed to be important for regular spatial o
74 ts, showing that microtubule linkages within asters are remarkably compliant (mean stiffness 0.025 pN
75 irst, the aggregation of microtubule foci or asters around the chromosomes, and second, the elongatio
76 ng and were specific for mitotic centrosomal asters as we observed little effect on interphase asters
77 lymerization of microtubules was measured in aster assays suggesting a role for MAP kinase in regulat
78 ved little effect on interphase asters or on asters assembled by the Ran-mediated centrosome-independ
79 h-TOGp is a major constituent of microtubule asters assembled in a mammalian mitotic extract and that
80 les in the microtubule pellet of the mitotic asters assembled in mammalian cell-free mitotic extract.
83 n mitotic extracts and reconstitutes mitotic aster assemblies in 4.1R-immunodepleted extracts in vitr
84 n vitro using a cell-free system for mitotic aster assembly and in vivo after injection into cultured
88 importin-beta is an inhibitor of microtubule aster assembly in Xenopus egg extracts and that Ran regu
89 oteins from the cell free system for mitotic aster assembly indicates that the plus end-directed acti
91 uRCs from centrosomes, inhibited microtubule aster assembly, and induced rapid disassembly of preasse
92 pADPr, extended from PARP-5a, also triggered aster assembly, suggesting a functional role of the pADP
98 function prevents the development of mitotic aster asymmetry and spindle pole movement towards the su
99 a key determinant in organizing microtubule aster asymmetry to power nuclear dynein-dependent separa
100 g the first phase (establishment), the sperm asters at one end of the embryo exclude the PAR-3/PAR-6/
103 broadened by attenuation of the centrosomal asters but was not affected by MP-GAP inhibition alone.
104 Here, we explore the contribution of the asters by analyzing the consequences of altering interas
109 lation is important to focus microtubules at aster centers and to facilitate the transition from aste
112 tubule (MT)-binding proteins, Orbit/multiple asters/cytoplasmic linker protein-associated protein, ha
113 gnaling-based cortical forces pulling on the asters, delays furrow formation and leads to the formati
115 ion from bull A resulted in an average sperm aster diameter of 101.4 microm (76.3% of oocyte diameter
116 ers (P < or = 0.0001) from the average sperm aster diameters produced after inseminations from bull B
117 anillin (ANI-1) promotes the formation of an aster-directed furrow in Caenorhabditis elegans embryos.
121 tile ring formation, with anaphase entry and aster disassembly also required for polar body biogenesi
122 onal alignment, we find that monopolar sperm asters do not show evidence for flux, partially contradi
124 (KIF2C) also resulted in ectopic microtubule asters during mitosis in C. elegans zygotes or HeLa cell
125 , we propose that signaling by the separated asters executes two critical functions: 1) it couples fu
127 We also show that (acentrosomal) microtubule asters fail to assemble in vitro without HSET activity,
128 In mutant embryos arrested in meiosis, sperm asters fail to form, and posterior is defined by the pos
130 rom Xenopus laevis eggs to study microtubule aster formation and microtubule dynamics in the presence
134 by specific antibodies impaired microtubule aster formation from purified mitotic centrosomes in vit
136 mation by blocking or reducing the degree of aster formation in chosen regions of the sample, without
139 increasing LIS1 concentration partly rescues aster formation, suggesting that Ndel1 is a recruitment
140 ized, tangle-shaped microtubules and reduced aster formation, which however did not alter appreciably
145 f an organized centrosome and its associated aster from one of the spindle poles, whereas the centros
148 rmal velocities, but reduced the ejection of asters from the spindles, blocked chromosome decondensat
150 growth is initiated by centrosomes but that asters grow by propagating a wave of microtubule nucleat
153 were also required for radial order of large asters growing in isolation, apparently to compensate fo
159 ch aster pairs from the same spindle (sister asters) have chromatin between them, whereas asters pair
161 icrotubules that either constitute the sperm aster in in vitro-fertilized (IVF) oocytes or originate
162 l F-actin ring that closely approximated the aster in location, measured diameter range, and pattern.
164 e addressed this question by imaging growing asters in a cell-free system derived from eggs, where as
166 to the mechanical properties of microtubule asters in a manner consistent with its localization to s
167 re, analysis of partially reconstituted MTOC asters in cells that escape complete repolymerization bl
169 an-GTP caused normal assembly of microtubule asters in depleted extracts, indicating that this defect
177 zation of Taxol-stabilized microtubules into asters in Xenopus meiotic extracts revealed motor-depend
178 ganized and centrosome-nucleated microtubule asters indicates that 4.1 is involved in regulating both
179 otofilament bundles emanating from different asters interconnect, mimicking the closure of the FtsZ r
180 monastral arrays of microtubules, the sperm aster is reduced in size, and the centrosomes often diss
183 en mating cells come into contact, they form aster-like actin structures that direct cell wall remode
184 in a mammalian mitotic extract organize into aster-like arrays in a centrosome-independent process th
187 Initially, FtsZ nucleation centers grow into aster-like structures that dramatically resemble microtu
192 ry revealed an important role of microtubule aster migration through cytoplasmic space, which depende
193 ve evolution, and we advocate for the use of aster modeling as a rigorous basis for achieving this go
196 Fus1, actin, and type V myosins revealed an aster of actin filaments whose barbed ends are focalized
199 m a cell free system for assembly of mitotic asters or antibody microinjection into cultured cells le
202 s as we observed little effect on interphase asters or on asters assembled by the Ran-mediated centro
203 ch noncentrosomal protein during microtubule aster organization and suggests that microtubule organiz
207 icrotubule plus-ends pushing the microtubule aster outward and that the balance of these forces posit
208 ation provides a natural experiment in which aster pairs from the same spindle (sister asters) have c
210 asters) have chromatin between them, whereas asters pairs from different spindles (nonsisters) do not
212 bly or taxol stabilization of the peripheral aster produced poorly defined rings or bulging anaphase
213 ation in vitro and in vivo and sequesters an aster promoting activity (APA) that consists of multiple
214 rm pronucleus and its associated centrosomal asters provide a cue that establishes the anterior-poste
215 that the large number of microtubules in the asters provides a highly precise mechanism for positioni
216 y (EPA) ASsessment Tool for Evaluating Risk (ASTER) QSAR (quantitative structure activity relationshi
220 y and promote the formation of interphase MT asters required for normal nuclear spacing, centrosome s
222 investigate the mechanism that keeps the two asters separate and forms a distinct boundary between th
227 s study describes a paternal effect on sperm aster size and microtubule organization during bovine fe
228 "early" step, manifested by greatly reduced aster size during early time points in maskin-depleted e
229 data support the hypothesis that peripheral aster spreading, perhaps dynein-driven, is causally rela
230 o quantify bundling in the whole microtubule aster structure and a way to compare the simulated resul
231 Retriever for Successful Revascularization (ASTER) study was a randomized, open-label, blinded end-p
232 phorylated APC/C associates with microtubule asters, suggesting that phosphatases are important.
233 uration during meiosis and growth of a sperm aster that could capture the oocyte meiotic spindle.
234 al model of the dynamic formin-filamin-actin asters that can self-organize into a contractile actomyo
236 gotes is positioned by two large microtubule asters that grow out from the poles of the first mitotic
237 ntation to the cell cortex using microtubule asters that grow out from the spindle poles during anaph
242 most feasible was found to be binding of the asters to cytoskeletal filaments and directed transport
243 modifies the landscape over time and allows asters to explore otherwise inaccessible configurations.
245 into a directional migration of centrosomal asters toward chromatin and their steady-state repositio
247 aying and attenuating the formation of sperm asters until after the period of reorganization, suggest
251 equired to organize acentrosomal microtubule asters, we show that addition of either active or kinase
252 d with NuMA and XMAP215 at the center of Ran asters where its activity is regulated by Aurora A-depen
253 is a radial array of microtubules called an aster, which is nucleated by a central organizing center
254 Like smaller cells, they are organized by asters, which grow, interact, and move to precisely posi
256 rsists and organizes an abnormal microtubule aster, while iMTOCs and satellites are greatly reduced.
257 losive transition from stationary to growing asters with a discontinuous jump of the aster velocity t
259 ced poorly defined rings or bulging anaphase asters within the ring center, respectively, inhibiting
261 and colleagues report that one PMU from the aster yellows phytoplasma strain Witches' Broom (AY-WB)
262 e 706,569-bp chromosome and four plasmids of aster yellows phytoplasma strain witches' broom (AY-WB)
263 all (+/- 10 kDa) virulence effector SAP11 of Aster Yellows phytoplasma strain Witches' Broom (AY-WB)
264 plete repeat among the PMUs in the genome of Aster Yellows phytoplasma strain Witches' Broom (AY-WB).
265 rabidopsis thaliana) expressing the secreted Aster Yellows phytoplasma strain Witches' Broom protein1
266 starvation responses, we found that secreted Aster Yellows phytoplasma strain Witches' Broom protein1
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