ライフサイエンスコーパス: astral microtubule

1 parallel with a second pathway that involves astral microtubules.

2 nents of the signaling pathway downstream of astral microtubules.

3 cal machine that mediates a pulling force on astral microtubules.

4 recruited, and thus modulate the behavior of astral microtubules.

5 proteins and large sizes, rely primarily on astral microtubules.

6 latter delivered cytoplasmic aurora B along astral microtubules.

7 tained collapsed spindles with numerous long astral microtubules.

8 ruption of the organization of dynein and/or astral microtubules.

9 d also suggested a possible interaction with astral microtubules.

10 xplained by the conventional model involving astral microtubules.

11 totic centrosomes is not dependent upon long astral microtubules.

12 endent pulling forces exerted on the tips of astral microtubules.

13 around the interior of the cell via dynamic astral microtubules.

14 m aster and in the organization of the sperm astral microtubules.

15 tor complex, which exerts a pulling force on astral microtubules.

16 plus-end tracking, including localization to astral microtubules.

17 bule organization and a dramatic increase in astral microtubules.

18 rients the spindle through interactions with astral microtubules.

19 nisms of mitotic spindle orientation rely on astral microtubules.

20 g by affecting the stability and dynamics of astral microtubules.

21 determined site of cytokinesis by pulling on astral microtubules.

22 rces that act between the cell periphery and astral microtubules.

23 ndle misorientation accompanied by shortened astral microtubules.

24 teractions of cortical molecular motors with astral microtubules.

25 rganize in vitro into asters, which resemble astral microtubules.

26 ion plane and a reduced number and length of astral microtubules.

27 ioles and are not anchored to the cortex via astral microtubules.

28 tic polarization via the central spindle and astral microtubules.

29 Astral microtubules, about four to six in number for eac

30 contain mitotic actin bundles, which prevent astral microtubule-actin cortex attachments.

31 Finally, dynein-dependent sliding of astral microtubules along the cortex and oscillation of

32 Astral microtubules also inhibit RhoA accumulation at th

33 hem as kinetochore (KMTs), spindle (SMTs) or astral microtubules (AMTs) according to their positions,

34 n, is a dynamic complex whose recruitment to astral microtubules (aMTs) increases dramatically during

35 ll cycle) contacting the bud by its existing astral microtubules (aMTs) while the new pole delays ast

36 in to the cell cortex followed by capture of astral microtubules (aMTs).

37 -300 nuclear ring and a polarized network of astral microtubules (aMTs).

38 position in concert with the position of the astral microtubule anchoring complex LGN-NuMA to yield t

39 rk in mitotic cells with an extension of the astral microtubules and a reduction of kinetochore micro

40 rnover of LGN relies, at least partially, on astral microtubules and DYNC1H1.

41 nted spindles were associated with disrupted astral microtubules and near complete loss of a unique s

42 (TCJs) localize force generators, pulling on astral microtubules and orienting cell division via the

43 e involved in generating forces that pull on astral microtubules and position the spindle asymmetrica

44 CLASPs act partially redundantly to regulate astral microtubules and position the spindle during asym

45 microtubules with a significant increase in astral microtubules and reduction in K-fiber fluorescenc

46 l deposition of new furrow membrane requires astral microtubules and release of internal stores of Ca

47 centrin-gamma TuRC interaction and perturbed astral microtubules and spindle bipolarity.

48 howed that spindle orientation required both astral microtubules and the actin cytoskeleton.

49 ed to be governed by the interaction between astral microtubules and the cell cortex and involve cort

50 itioning depends on interactions between the astral microtubules and the cell cortex.

51 (+) plays a role in interactions between the astral microtubules and the cortical cytoskeleton.

52 enter to the cortex, a process that requires astral microtubules and the microtubule-based motor dyne

53 Cytokinesis is regulated by both astral microtubules and the midzone microtubules of the

54 molecular mechanism for the organization of astral microtubules and the mitotic spindle through Rab1

55 elial cells resulted in the disappearance of astral microtubules, and dividing spindle fiber formatio

56 short mitotic spindles, increased numbers of astral microtubules, and require the presence of the kin

57 As centrosome integrity and astral microtubules appeared unperturbed by (+)-1,2-bis(

58 While astral microtubules are believed to be primarily respons

59 The majority of astral microtubules are dynamically unstable.

60 Although astral microtubules are known to play roles in these pro

61 Our results indicate that astral microtubules are required for establishing bipola

62 physical centrosome removal demonstrate that astral microtubules are required for such spindle elonga

63 In addition, dynein-mediated forces on astral microtubules are sufficient to segregate a 2N chr

64 it as astrin because of its association with astral microtubule arrays assembled in vitro.

65 ntained bipolar spindles with dense and long astral microtubule arrays but with poorly organized kine

66 ction into cultured cells leads to organized astral microtubule arrays with expanded polar regions in

67 racted to form spindles joined in series via astral microtubules as revealed by live cell imaging.

68 he stabilization of cortical associations of astral microtubules at cell-cell adhesions to orient the

69 to examine the residence time of individual astral microtubules at the cell cortex of developing emb

70 raction at SPBs compromises the anchorage of astral microtubules at the SPB and surprisingly also inf

71 lin and late stage embryos often do not have astral microtubules at their spindle poles.

72 orylates MISP, thus stabilizing cortical and astral microtubule attachments required for proper mitot

73 e orientation via regulation of actin cortex-astral microtubule attachments.

74 s have shown that cortical actin may mediate astral microtubule-based movements of the mitotic spindl

75 ryogenesis is characterized by shifting from astral microtubule-based to central spindle-based positi

76 regions of the cell cortex that overlap with astral microtubules become enriched in actin and coronin

77 Comparison of astral microtubule behavior in mitosis with microtubule

78 f, while making contact with the cortex, the astral microtubules buckle as they exert compressive, pu

79 h MUG and normal mitosis, chromatin attracts astral microtubules but cannot induce spindle assembly.

80 om the cell cortex through interactions with astral microtubules, but neither the mechanism governing

81 bules nucleated along the length of existing astral microtubules, but this increase negatively affect

82 ugh the capture and subsequent shortening of astral microtubules by a site in the cortex.

83 absence of a functional exclusion mechanism, astral microtubules can associate with Pins over the ent

84 tic centrosomes and has few or no detectable astral microtubules, can develop into an adult fly.

85 entrosomes lose gamma-tubulin, spindles lose astral microtubules, chromosomes fail to reach a metapha

86 Remarkably, cells depleted of astral microtubules conduct accurate, complete cytokines

87 nd positioning around chromosomes depends on astral microtubule connections to a moving cell cortex.

88 These forces act before astral microtubules contact the cortex; thus, dynein mus

89 During mitosis, spindle poles align so the astral microtubules contact the lateral cortex.

90 cortical dynein-generated pulling forces on astral microtubules contribute to anaphase spindle elong

91 same cytokinetic signaling ensemble-opposed astral microtubules decorated with Ect2 and Cyk4.

92 We show that reducing astral microtubules decreases the frequency of spindle m

93 dominant-negative Rab11 expression disrupts astral microtubules, delays mitosis, and redistributes s

94 interactions with mutations that deactivate astral microtubule-dependent nuclear migration.

95 Caenorhabditis elegans one-cell embryo, the astral microtubule-dependent pathway requires anillin, N

96 Before cellularization, astral microtubules determine metaphase furrow position

97 ow the cortex causes the depolymerization of astral microtubules during asymmetric spindle positionin

98 obtain time-lapse recordings of fluorescent astral microtubule dynamics and nuclear movements over t

99 Astral microtubule dynamics are critical to the mechanis

100 yeast, Schizosaccharomyces pombe, depend on astral microtubule dynamics that drag the nucleus throug

101 ctility both preceded and was independent of astral microtubule elongation, suggesting that the initi

102 held assumptions that the centrosome and the astral microtubules emanating from it are essential for

103 The distance between astral microtubules ends and the anterior cortex was sig

104 contact geometry from "end-on" to "side-on." Astral microtubules engage cortically anchored motors al

105 rimary function of centrosomes is to provide astral microtubules for proper nuclear spacing and migra

106 Delta and gfh1Delta cells exhibit defects in astral microtubule formation and anchoring, suggesting t

107 Simultaneous contact of astral microtubules from both poles with the AMIZ direct

108 hate-bound Ran, stimulated polymerization of astral microtubules from centrosomes assembled on Xenopu

109 Astral microtubules from one pole only then contact the

110 or EB1 in processes that promote equality of astral microtubule function at both poles in a spindle.

111 g that these proteins have specific roles in astral microtubule function.

112 this amino acid, plays a role in a subset of astral microtubule functions during nuclear migration in

113 Astral microtubules grow out from the two spindle poles,

114 eck in S/G2 or G2/M was dependent on dynamic astral microtubules growing into the bud.

115 bly, although it is required for cytoplasmic astral microtubule growth during mitosis.

116 e nucleus moves in the opposite direction of astral microtubule growth in the mother cell, apparently

117 g that Ect2 migrates from spindle midzone to astral microtubules in anaphase and that Ect2 shapes the

118 port a role for both the central spindle and astral microtubules in cytokinesis in animal cells.

119 the first views of the dynamic properties of astral microtubules in live budding yeast.

120 sh embryos, where cells are unusually large, astral microtubules in metaphase are too short to positi

121 dundantly to suppress the formation of basal astral microtubules in neuroblasts.

122 robably a direct result of a failure to form astral microtubules in parthenogenetic embryos lacking c

123 r RNAi leads to a reduction in the length of astral microtubules in syncytial embryos, larval neurobl

124 the actomyosin cytoskeleton to plus ends of astral microtubules in the equatorial region of the cell

125 Because the plus-ends of many astral microtubules in the spindle point to the cell cor

126 es delayed development and a failure to form astral microtubules in the zygote.

127 000, consistent with the several thousand of astral microtubules in these cells.

128 absence of Cdc28-Clb4 activity (G1/S phase), astral microtubules interact with the bud tip in a manne

129 Dynamic plus ends of astral microtubules interact with the shmoo tip in matin

130 n surrounding the nucleus, which we term the astral microtubule interaction zone (AMIZ).

131 ctin cables are important for either guiding astral microtubules into the bud or anchoring them in th

132 eavage plane geometry in which the length of astral microtubules is limited by interaction with these

133 ted at the cell cortex to generate forces on astral microtubules is not clear.

134 We show that astral microtubules, kinesin Khc-73, and Discs large (Dl

135 Rather, confinement increases numbers of astral microtubules laterally contacting the cortex, shi

136 Disruption of astral microtubules leads to misalignment of the spindle

137 s to regulate cell cycle-specific changes in astral microtubule length to ensure proper spindle align

138 on we found that cenexin depletion decreased astral microtubule length, disrupted astral microtubule

139 equired for proper chromosome separation and astral microtubule length.

140 otic cells, including a drastic reduction in astral microtubules, malformed mitotic spindles, defocus

141 l release and transport of LGN complex along astral microtubules may contribute to spindle positionin

142 Transport on astral microtubules may provide a general mechanism for

143 Reestablishment of astral microtubule-mediated forces align the spindle pol

144 We provide evidence for dynein- and astral microtubule-mediated transport of Galphai/LGN/nuc

145 iated role for the Astrin/SKAP complex as an astral microtubule mediator of mitotic spindle positioni

146 We report that even in the absence of astral microtubules, metaphase spindles in MDCK and HeLa

147 creased astral microtubule length, disrupted astral microtubule minus-end organization, and increased

148 In contrast, mEg5 punctae on astral microtubules moved transiently toward microtubule

149 al imaging microscopy to examine cytoplasmic astral microtubules (Mts) and spindle dynamics during th

150 they grow, their minus ends are captured by astral microtubules (MTs) and transported poleward throu

151 Astral microtubules (MTs) are known to be important for

152 Contacts between cytoplasmic dynein and astral microtubules (MTs) at the cell cortex generate pu

153 cess begins with the capture of pole-derived astral microtubules (MTs) by the polarity determinant Bu

154 Astral microtubules (MTs) emanating from the mitotic app

155 e positioning in budding yeast by pulling on astral microtubules (MTs) from the cell cortex.

156 A), proteins that generate pulling forces on astral microtubules (MTs) through cytoplasmic dynein.

157 ynactin was sufficient to generate forces on astral microtubules (MTs) to orient the spindle, with Nu

158 ates MF-dependent cortical forces applied to astral microtubules (MTs).

159 nuclear complex (PNC) and its two associated astral microtubules (MTs).

160 though these cells lack the radial arrays of astral microtubules normally associated with each spindl

161 Moreover, astral microtubule number and length correlated with the

162 process thought to include direct capture by astral microtubules of kinetochores and/or noncentrosoma

163 (dynein-GFP), which fluorescently labels the astral microtubules of the budding yeast Saccharomyces c

164 yeast spindle pole body [SPB]) nucleate more astral microtubules on one of the two spindle poles than

165 Loss of either astral microtubules or Lis1/dynactin leads to spindle/co

166 primed to form furrows at either overlapping astral microtubules or the central spindle.

167 of SPB maturation, control the asymmetry of astral microtubule organization between the preexisting

168 ove the differential activity of the SPBs in astral microtubule organization rather than intrinsic di

169 icrotubules (aMTs) while the new pole delays astral microtubule organization.

170 NA is involved in negative regulation of the astral microtubule organizing capacity of the spindle po

171 The astral microtubule organizing function of Nud1p is media

172 ), Polo, and Fascetto (Prc1) localize to the astral microtubule overlap region.

173 As nuclei divide, continued transport on astral microtubules partitions germ plasm to daughter nu

174 gly, most of the suppressors that rescue the astral microtubule phenotype also reduce Cdk1-CycB activ

175 by gating the recruitment of dynactin to the astral microtubule plus end, a prerequisite for offloadi

176 Khc-73 localizes to astral microtubule plus ends, and Dlg/Khc-73 and Dlg/Pin

177 Cortical pulling on astral microtubules positions the mitotic spindle in res

178 ng abnormally stretched or fragmented due to astral microtubule pulling forces.

179 mal in these embryos, but reduced numbers of astral microtubules reach all regions of the cortex at t

180 ex and allow dynein to produce forces on the astral microtubules required for mitotic spindle alignme

181 cally, constitutively active Rab11 increases astral microtubules, restores gamma-tubulin spindle pole

182 A subset of astral microtubules stabilizes during anaphase, becoming

183 Second, astral microtubules, structures that undergo similar pat

184 e at approximately 11 microm/min, similar to astral microtubules, suggesting polymerization velocity

185 ely associated with both the cell cortex and astral microtubules, suggesting that it may directly int

186 cytoplasmic CENP-E toward chromosomes along astral microtubules that enter the nuclear volume.

187 the most dramatic effect on the lifetimes of astral microtubules that extend toward the cell cortex.

188 le treatment revealed a population of stable astral microtubules that formed during anaphase; among t

189 The long astral microtubules that occur in the absence of Kif18B

190 However, mitotic C377S tub1 cells displayed astral microtubules that often appeared excessive in num

191 phases due to a tethering force, mediated by astral microtubules that reach the anterior cell cortex.

192 ocess is likely driven by interactions among astral microtubules, the motor protein dynein, and the c

193 Considerations of the length of the astral microtubules, their diamagnetic anisotropy, and f

194 ed through a cortical machinery by capturing astral microtubules, thereby generating pushing/pulling

195 brane, where dynein captures and walks along astral microtubules to help orient the mitotic spindle.

196 The dynein/dynactin motor complex pulls astral microtubules to orient the spindle.

197 ar mitotic apparatus protein (NuMA)-positive astral microtubules to orientate the mitotic spindle.

198 chromosomes during anaphase, cooperates with astral microtubules to position the cleavage furrow.

199 anchored dynein generates pulling forces on astral microtubules to position the mitotic spindle acro

200 c delay and prevents appropriate assembly of astral microtubules to promote spindle misorientation.

201 However, the motors that pull astral microtubules toward these actin structures are no

202 one spindle pole body the task of organizing astral microtubules towards the mother cell.

203 eted embryos, but the polymerization rate of astral microtubules was not slower than in wild type.

204 eation rate in LLCPK cells and the number of astral microtubules was similar in stathmin +/+ and -/-

205 rosome and a subset of differentially stable astral microtubules were also observed.

206 ligned in latrunculin-treated cells and that astral microtubules were misoriented, confirming a role

207 er to move from this SPB to the plus ends of astral microtubules, where Cdc28-Clb4 regulates the inte

208 nhibition of dynein blocked mEg5 movement on astral microtubules, whereas depletion of the Eg5-bindin

209 l and cellular events, perhaps by nucleating astral microtubules which mediate interactions with othe

210 gamma-tubulin complex organizes spindle and astral microtubules, which, in turn, separate replicated

211 e alignment by regulating the interaction of astral microtubules with subdomains of the bud cortex.

212 tioned and oriented by interactions of their astral microtubules with the cellular cortex, followed b

213 rotation is dependent on the interaction of astral microtubules with the cortical actin cytoskeleton

214 rst, Peg1 was required to form a spindle and astral microtubules, yet destabilized interphase microtu