ライフサイエンスコーパス: atrial myocyte

1 orating a detailed ionic-current model of an atrial myocyte.

2 vations about the spread of Ca(2+) within an atrial myocyte.

3 nt within the three-dimensional volume of an atrial myocyte.

4 ptor clusters within a specific z disk of an atrial myocyte.

5 shown that SR Ca content is increased in old atrial myocytes.

6 monstrated TREK-1 protein in the membrane of atrial myocytes.

7 gnificantly smaller in +LMN compared to -LMN atrial myocytes.

8 alpha-adrenergic and cholinergic signals in atrial myocytes.

9 tois by specialized secretory cells, such as atrial myocytes.

10 ling of Kv1.5 in the HL-1 immortalized mouse atrial myocytes.

11 and an attenuation of I(Ca,L) in the canine atrial myocytes.

12 s can trigger arrhythmias in ventricular and atrial myocytes.

13 obal Ca2+ increase and muscle contraction in atrial myocytes.

14 tion of RyR2 clusters in rat and ventricular atrial myocytes.

15 .87 microm in ventricular and 1.69 microm in atrial myocytes.

16 icular myocytes and estimated 0.97 microm in atrial myocytes.

17 e voltage-dependent activation of I(Ca,L) in atrial myocytes.

18 (PE) on L-type Ca2+ current (I(Ca,L)) in cat atrial myocytes.

19 central release of Ca(2+) in this subset of atrial myocytes.

20 is the predominant IP3R isoform expressed in atrial myocytes.

21 e and uptake in intact and permeabilized cat atrial myocytes.

22 were not different in WT and IP3R2-deficient atrial myocytes.

23 neous Ca2+ release events in IP3R2-deficient atrial myocytes.

24 diversity of local Ca(2+) signalling in rat atrial myocytes.

25 oline (ACh) on intracellular NO (NOi) in cat atrial myocytes.

26 Ca(2+) release in subsarcolemmal domains of atrial myocytes.

27 te exposure to thyroid hormone (T(3)) on cat atrial myocytes.

28 to action potential repolarization of human atrial myocytes.

29 ve changes in the calcium handling system of atrial myocytes.

30 (2)-adrenergic receptor (AR) agonists in cat atrial myocytes.

31 with a loss of carbachol-induced current in atrial myocytes.

32 anisms of cardiac alternans in single rabbit atrial myocytes.

33 tivated, or T-type Ca(2+), channel in murine atrial myocytes.

34 lation of L-type Ca(2+) current (I(Ca,L)) in atrial myocytes.

35 icantly inhibited ICl,vol in most guinea-pig atrial myocytes.

36 patches from acutely dissociated guinea-pig atrial myocytes.

37 nt atrial pacemaker cells but not in working atrial myocytes.

38 from the IP3 R2 in the peripheral domains of atrial myocytes.

39 ar stress-sensitive current (Ishear ) in rat atrial myocytes.

40 key component of parasympathetic synapses on atrial myocytes.

41 ed in discrete bundles that appeared to abut atrial myocytes.

42 id communication and coordinate responses of atrial myocytes.

43 s a low voltage-activated calcium channel in atrial myocytes.

44 duced delayed afterdepolarizations (DADs) in atrial myocytes.

45 (ANP) is present in caveolae of in situ rat atrial myocytes.

46 d atrial myocytes and of freshly dissociated atrial myocytes.

47 rinic stimulation of native GIRK currents in atrial myocytes.

48 n ventricular myocytes were also observed in atrial myocytes.

49 dine block of IKr and IKur currents in human atrial myocytes.

50 pe-mediated Ca(2+) current in EHD3-deficient atrial myocytes.

51 also had opposing effects on ICa,L in human atrial myocytes.

52 ANP had any effect on Na(+) current in mouse atrial myocytes.

53 regulation controlling the repolarization of atrial myocytes.

54 1 expression in enzymatically isolated human atrial myocytes.

55 Ca2+ signaling in isolated rabbit and human atrial myocytes.

56 region of the SHF gives rise to both OFT and atrial myocytes.

57 ed ACs are functionally active in guinea-pig atrial myocytes.

58 K,ACh) is opposite to its effect on I(K1) in atrial myocytes.

59 ependent on CaMKII) that regulates I(CaL) in atrial myocytes.

60 (2+) release were recorded in isolated human atrial myocytes.

61 channel membrane targeting and regulation in atrial myocytes.

62 lexes among different SK channel subunits in atrial myocytes.

63 were altered consistently in ST8sia2((-/-)) atrial myocytes.

64 nd outward currents during repolarization in atrial myocytes.

65 of SK2 channels in cardiac repolarization in atrial myocytes.

66 ith the Cav3.1 channel in both HEK cells and atrial myocytes.

67 nt gap junction protein that is expressed in atrial myocytes.

68 We conclude that in cat atrial myocytes, ACh stimulates NOi release from local s

69 WT and IP3R2-deficient atrial myocytes also showed a significant and very simil

70 The canonical atrial myocyte (AM) is characterized by sparse transvers

71 nized transverse tubules (T-tubules) such as atrial myocytes (AMs).

72 luciferase activity in cultured neonatal rat atrial myocytes, an effect that was increased to 8-9-fol

73 PDE4 is expressed in human atrial myocytes and accounts for approximately 15% of to

74 KACh) deactivation kinetics in both neonatal atrial myocytes and adult sinoatrial nodal cells.

75 channels (SK, KCa 2) are expressed in human atrial myocytes and are responsible for shaping atrial a

76 ecreased L-type calcium current (I(Ca,L)) in atrial myocytes and decreased atrial contractility.

77 S107 reduced the diastolic SR Ca2+ leak in atrial myocytes and decreased burst pacing-induced AF in

78 Electrotonic coupling between atrial myocytes and Fbs may contribute to the formation

79 s the timing of parasympathetic influence on atrial myocytes and heart rate in mice.

80 eceptor when tested in cultured neonatal rat atrial myocytes and HeLa cells.

81 2+) signals ([Ca(2+)](Nuc)) in permeabilized atrial myocytes and in isolated cardiac nuclei.

82 via pertussis toxin-sensitive G proteins in atrial myocytes and in many neuronal cells.

83 of SCaEs and delayed afterdepolarizations in atrial myocytes and intact atria and prevented induction

84 potassium channel forms the IKur current in atrial myocytes and is functionally altered by coexpress

85 NP colocalize at the plasmalemma of cultured atrial myocytes and of freshly dissociated atrial myocyt

86 tes the I(Kur) repolarizing current in human atrial myocytes and regulates vascular tone in multiple

87 We used isolated rat atrial myocytes and related changes in their subcellular

88 nside the cells; they are expressed in human atrial myocytes and responsible for shaping atrial actio

89 the gamma(6) subunit is highly expressed in atrial myocytes and that it is capable of acting as a ne

90 the density of K+ currents in left and right atrial myocytes and the density of delayed rectifier K+

91 Ca(2+) spark frequency in atrial myocytes and the open probability of single RyR2

92 transfection of mouse C2C12 myoblasts, HL-1 atrial myocytes, and c-kit(+) cardiac progenitor cells d

93 welling-activated Cl- currents in guinea pig atrial myocytes, and Ca(2+)-activated Cl- currents in ca

94 g in high sensor expression in arterial ECs, atrial myocytes, and cardiac Purkinje fibers.

95 nt (Ik(ur)), a major repolarizing current in atrial myocytes, and regulating the resting membrane pot

96 Ankyrin-B is expressed in atrial myocytes, and we demonstrate its requirement for

97 etected in approximately 40 % of adult mouse atrial myocytes, and when expressed, the density of this

98 These experimental designs tested whether atrial myocyte ANP-RB colocalizes at the plasmalemma and

99 mANP (10-100 nmol/L) had opposing effects on atrial myocyte AP morphology and ICa,L.

100 Atrial myocytes are continuously exposed to mechanical f

101 trated that the functions of SK2 channels in atrial myocytes are critically dependent on the normal e

102 e properties of Ito,f and Iss in adult mouse atrial myocytes are similar to those of the analogous cu

103 Atrial myocytes are subjected to shear stress during the

104 T-current density that normally results when atrial myocytes are treated with insulin-like growth fac

105 g) was studied in isolated fluo-3-loaded rat atrial myocytes at 22 and 37 degrees C using rapid confo

106 ctance is essentially abolished in SUR1(-/-) atrial myocytes but is normal in SUR1(-/-) ventricular m

107 IK,ACh in uninfected and freshly dissociated atrial myocytes but the effect was larger and more consi

108 ould be stimulated in the central regions of atrial myocytes by application of 2.5 mM caffeine.

109 ediated Ca2+ influx in isolated canine right atrial myocytes by approximately 60%, but had no signifi

110 in adult isolated canine ventricular and and atrial myocytes by using whole-cell and perforated-patch

111 etion also reduced Kv currents in male mouse atrial myocytes, by >45% (P < 0.001).

112 ted in the HL-1 cell line derived from mouse atrial myocytes, by using small interfering RNA knockdow

113 oach successfully reproduces key features of atrial myocyte Ca(2+) signaling observed using confocal

114 ABSTRACT: In atrial myocytes Ca(2+) release during excitation-contrac

115 ERCA function was altered to reproduce human atrial myocyte Ca2+ transients.

116 Ferret atrial myocytes can display an E-4031-sensitive current

117 helin-1 (ET-1) stimulation of wild-type (WT) atrial myocytes caused an increase in basal [Ca2+]i, an

118 sed the increase in K+ current of guinea pig atrial myocytes caused by 100 microM adenosine (259 +/-

119 ed in the cytoplasmic microdomain underlying atrial myocyte caveolae may be the activation of cGMP-de

120 ification and [K+]o sensitivity in the mouse atrial myocyte cell line, AT-1 cells.

121 We established atrial myocyte cell lines expressing siRNA against desmo

122 tial distribution of Cav1.3 Ca2+ channels in atrial myocytes compared with ventricles.

123 .5 contribute to distinct K+ currents in rat atrial myocytes demonstrates that Kv1.2 and Kv1.5 also d

124 In HF atrial myocytes diastolic [Ca(2+)]i was increased, actio

125 We conclude that rat atrial myocytes display a predetermined spatiotemporal p

126 Moreover, ankyrin-B(+/-) atrial myocytes display shortened action potentials, con

127 Ca(2+) signals in indo-1- and fluo-4-loaded atrial myocytes during electrical pacing.

128 e centripetal Ca(2+) waves that occur within atrial myocytes during excitation-contraction coupling,

129 of Ca(2+) alternans in field-stimulated cat atrial myocytes employing fast two-dimensional fluoresce

130 In intact cat atrial myocytes, endothelin (ET-1) increased basal [Ca(2

131 In approximately 30 % of the atrial myocytes examined, 8-Br-cAMP increased macroscopi

132 te EHD3 as a key player in the regulation of atrial myocyte excitability and cardiac conduction.

133 KEY POINTS: In atrial myocytes excitation-contraction coupling is strik

134 S6 in G protein inactivation, RGS6-deficient atrial myocytes exhibited a significant reduction in the

135 investigate whether intracaveolar ANP of rat atrial myocytes exists within caveolae bound to type B A

136 nctions as a dominant negative, and from P15 atrial myocytes exposed to (1 microM) antisense oligodeo

137 urrents were attenuated significantly in rat atrial myocytes exposed to AsODNs targeted against Kv4.2

138 Atrial myocytes express three different gap junction cha

139 In this study, treatment of HL-1 atrial myocytes expressing Kv1.5-GFP with the class I an

140 In cultured adult rabbit left atrial myocytes, expression of S140G-IKs shortened actio

141 As with acetylcholine-activated current in atrial myocytes, external Cs+ blocked inward but not out

142 Immunostaining atrial myocytes for type II ryanodine receptors (RyRs) r

143 Atrial myocytes, for example, coexpress connexin (Cx) 40

144 ce microscopy applied to primary cultures of atrial myocytes from adult rats and to freshly dissociat

145 Incubation of atrial myocytes from AF patients (but not controls) with

146 oltage-gated outward K+ current densities in atrial myocytes from AF patients demonstrates the need t

147 Whole-cell patch-clamp studies of atrial myocytes from Akita mice exhibited a markedly dec

148 In vitro I-1c gene transfer in isolated left atrial myocytes from both pigs and rats increased calciu

149 excitation-contraction coupling in isolated atrial myocytes from cat heart.

150 nts (I(Ca,L)) recorded from single, isolated atrial myocytes from Cav1.3(-/-) mice showed a significa

151 Atrial myocytes from FKBP12.6-/- mice exhibited spontane

152 spark frequency, SR Ca(2+) leak, and DADs in atrial myocytes from FKBP12.6-/-:S2814A mice compared wi

153 ion, the data support the hypothesis that in atrial myocytes from hearts with left ventricular failur

154 is showed that miR-21 was expressed in human atrial myocytes from patients in sinus rhythm and that i

155 gical K(2P)3.1 inhibition prolonged APD90 in atrial myocytes from patients with chronic AF to values

156 I KACh, attenuated in atrial myocytes from SREBP-1 knockout mice, was stimulat

157 rominently in late cardiac repolarization in atrial myocytes from the heterozygous and homozygous nul

158 ced spontaneous diastolic Ca(2+) releases in atrial myocytes from the patients with SR that were abol

159 blotting) in tissue homogenates and isolated atrial myocytes from the right atrial appendage (RAA) of

160 n of GIRK1, SREBP-1, and I(KAch) activity in atrial myocytes from these mice to levels in wild-type m

161 isoprenaline-mediated regulation of I Ks in atrial myocytes from transgenic but not WT littermates.

162 (ICa,L), and Na(+) current were recorded in atrial myocytes from wild-type or natriuretic peptide re

163 Using single atrial myocytes from young and old Welsh Mountain sheep,

164 Atrial myocytes generally lack transverse tubules; howev

165 The results indicate that in atrial myocytes glycolysis regulates Ca(2+) release from

166 Isolated hypoxic atrial myocytes had 43% fewer dense surface secretory gr

167 To represent the system, a human atrial myocyte (hAM) coupled to a variable number of Fbs

168 Atrial myocytes have two functionally separate Ca2+ rele

169 or Ca(2+)-stimulated AC in the regulation of atrial myocyte I(CaL).

170 In atrial myocytes immunocytochemistry has shown two groups

171 granules and degenerative myelin figures in atrial myocytes; immunogold studies localized Rab1a to t

172 In contrast to atrial myocytes, in mink lung epithelial cells, in which

173 of gene expression similar to that of adult atrial myocytes, including expression of alpha-cardiac m

174 regular-type action potentials of PMCA1(cko) atrial myocytes increased significantly under Ca(2+) ove

175 tion and early after-depolarization in human atrial myocytes, increasing vulnerability to stress-prov

176 Recent experiments in atrial myocytes indicate that withdrawal of cholinergic

177 Similar results were obtained in +LMN atrial myocytes infected with Adv-FRNK.

178 ervations support the hypothesis that in rat atrial myocytes, intracaveolar ANP is bound to ANP-RB, a

179 The atrial natriuretic peptide secreted by atrial myocytes is a major adipogenic factor operating a

180 r the inwardly rectifying K+ current IK1, in atrial myocytes is affected by the expression of Kv4.2W3

181 that RyR2-mediated diastolic SR Ca2+ leak in atrial myocytes is associated with AF in CPVT mice.

182 In type 2 IP3 R (IP3 R2) knockout atrial myocytes, Ishear was 10-20% of that in wild-type

183 t of AF on human I(Ca), we compared I(Ca) in atrial myocytes isolated from 42 patients in normal sinu

184 Voltage-clamp studies on atrial myocytes isolated from adult and postnatal day 15

185 GIRK1 appeared intracellular in atrial myocytes isolated from GIRK4 knockout mice and wa

186 nt densities are attenuated significantly in atrial myocytes isolated from P15 and adult Kv2.1N216Fla

187 tward K+ current densities in left and right atrial myocytes isolated from patients in chronic AF, re

188 was a significant diastolic SR Ca2+ leak in atrial myocytes isolated from the CPVT mouse models.

189 veal that Ito,f is selectively eliminated in atrial myocytes isolated from transgenic mice expressing

190 In canine atrial myocytes, isoprenaline (1 microM) consistently re

191 strikingly different from ventricle because atrial myocytes lack a transverse tubule membrane system

192 In many species atrial myocytes lack a transverse tubule system, dividin

193 The finding that cat atrial myocytes lack t-tubules demonstrates the function

194 Cat atrial myocytes lack transverse tubules and contain sarc

195 acilitating the propagation of Ca2+ waves in atrial myocytes lacking t-tubular system and provide the

196 Atrial myocytes, lacking t-tubules, have two functionall

197 In embryonic chick atrial myocytes, lipid lowering by culture in lipoprotei

198 lated areas and markedly depressed in viable atrial myocytes near the ablation zones (group 1).

199 These results indicate that in atrial myocytes, NO released by selective beta(2)-AR sti

200 ndant apoJ mRNA and protein are expressed in atrial myocytes; no expression is detected in ventricula

201 om adult rats and to freshly dissociated rat atrial myocytes (not cultured).

202 In human atrial myocytes obtained from patients in sinus rhythm,

203 smic reticulum Ca(2+)-leak were increased in atrial myocytes of miR-106b-25(-/-) mice.

204 eported that short-term (2 h) plating of cat atrial myocytes on the extracellular matrix protein, lam

205 ormal excitation-contraction coupling in cat atrial myocytes, only Ca(2+) release from the j-SR is di

206 We conclude that in cat atrial myocytes PE acts via alpha1-ARs coupled to PTX-in

207 Studies were performed on acutely isolated atrial myocytes plated on uncoated coverslips (LMN) or c

208 ed in part on a previous model of the rabbit atrial myocyte published by our group and was motivated

209 Moreover, loss of ankyrin-B in atrial myocytes results in decreased Ca(v)1.3 expression

210 ecordings from isolated adult (C57BL6) mouse atrial myocytes reveal the presence of two prominent Ca2

211 electron micrographs of thin sections of rat atrial myocytes revealed a fraction of dystrophin molecu

212 -SR ([Ca(2+) ]SR ; fluo-5N) Ca(2+) in rabbit atrial myocytes revealed that Ca(2+) release from j-SR r

213 Immunocytochemical analysis of cultured rat atrial myocytes revealed that T-cadherin and caveolin ha

214 y, adenoviral expression of SREBP-1 in Akita atrial myocytes reversed the impaired I(KAch) to levels

215 ctedly leads to increased outward current in atrial myocytes, shortens atrial action potentials, and

216 Therefore all G protein-coupled receptors in atrial myocytes should be able to activate IK,ACh.

217 Electron micrographs of atrial myocytes show peripheral SR cisternae in close pr

218 tion (100 nM isoproterenol), only Casq2(-/-) atrial myocytes showed pacing-induced self-sustained rep

219 e SK channels are predominantly expressed in atrial myocytes, specific ligands of the different isofo

220 Insulin treatment of cultured atrial myocytes stimulated GIRK1 expression 2.68+/-0.12-

221 lting in electrophysiological alterations in atrial myocytes that may promote AF.

222 tion-PCR to identify partial clones from rat atrial myocytes that share high homology with a member o

223 he results demonstrate directly that, in cat atrial myocytes, the action potential-induced whole-cell

224 However, in atrial myocytes, the effects of shear stress are poorly

225 of the delayed rectifier K+ current in human atrial myocytes, the ultrarapid delayed rectifier K+ cur

226 In adult rat atrial myocytes, three kinetically distinct Ca2+-indepen

227 at beta-adrenoceptors can activate IK,ACh in atrial myocytes through the release of betagamma subunit

228 ectively, but did not alter the responses of atrial myocytes to carbachol.

229 (2+) buffers (EGTA) into voltage-clamped rat atrial myocytes to isolate the fast component of central

230 rated that TGFbeta regulates the response of atrial myocytes to parasympathetic stimulation.

231 We previously reported that attachment of atrial myocytes to the extracellular matrix protein lami

232 ol was significantly prolonged in PMCA1(cko) atrial myocytes under basal conditions, with Ca(2+) over

233 g and during Ca(2+) alternans was studied in atrial myocytes using fast confocal microscopy and measu

234 e shear stress-sensitive membrane current in atrial myocytes using the whole-cell patch clamp techniq

235 the central (CT) region of the cell) of cat atrial myocytes using whole-cell voltage-clamp together

236 l alpha1C-unassociated Ca2+-release sites of atrial myocytes, using rapid (240 Hz) two-dimensional co

237 and peripheral sites of voltage-clamped rat atrial myocytes, using rapid 2-dimensional (2-D) confoca

238 currents (Ito, IKur, Ins, and IK1) in human atrial myocytes, using the whole-cell configuration of t

239 activities of three myocyte subpopulations: atrial myocytes, ventricular myocytes, and cells of the

240 fact, for select cardiac cell types such as atrial myocytes, virtually nothing is known regarding en

241 It is concluded that in atrial myocytes voltage-dependent Ca2+ entry triggers Ca

242 re, the average RMP of embryonic day (ED) 11 atrial myocytes was -22 +/- 2 mV.

243 Native I(f) in nonexpressed atrial myocytes was 7+/-4 pA at -130 mV (n=5), whereas H

244 Basal activity of an AC in isolated atrial myocytes was demonstrated by the observations tha

245 bound gp91phox containing NAD(P)H oxidase in atrial myocytes was the main source of atrial superoxide

246 two-dimensional confocal Ca2+ imaging in rat atrial myocytes, we examine directly the role of I(Ca) o

247 e TATS in excitation-contraction coupling in atrial myocytes, we visualized the TATS (labelled with t

248 Mechanical properties of transgenic atrial myocytes were enhanced to the level of ventricula

249 Atrial myocytes were plated for at least 2 h on uncoated

250 Atrial myocytes were pretreated with vehicle (control) o

251 ency of spark occurrence in the periphery of atrial myocytes where the native alpha1C-RyR complexes a

252 In membrane-permeabilized (saponin-treated) atrial myocytes, where [Ca2+] can be experimentally cont

253 determining membrane potential in embryonic atrial myocytes, where I(K1) is absent.

254 excitation-contraction (E-C) coupling in cat atrial myocytes which lack transverse tubules and contai

255 action was also demonstrated in chick embryo atrial myocytes (which do not express endogenous A(3) re

256 s block the Ca(2+)-activated K(+) current in atrial myocytes, which is important for cardiac repolari

257 The data suggest that in atrial myocytes, which lack a t-tubular system, the nj-S

258 In atrial myocytes, which lack t-tubules, ICa inactivation

259 Pretreatment of atrial myocytes with 10 nM FSCPX reduced the maximal act

260 imulation of quiescent or electrically paced atrial myocytes with a membrane-permeant InsP3 ester, wh

261 Co-immunostaining of atrial myocytes with antibodies against type II ryanodin

262 Treatment of cultured atrial myocytes with CNTF resulted in a twofold increase

263 type Ca2+ current (beta-ICa,L) of guinea pig atrial myocytes with EC50 values of 2.17 and 0.20 microM

264 Pretreatment of atrial myocytes with FSCPX (50 nM) markedly attenuated t

265 tes lacking t-tubular system and provide the atrial myocytes with functional Ca2+ signaling diversity

266 Treatment of isolated atria or cultured atrial myocytes with recombinant human or avian CNTF res

267 We found two populations of rat atrial myocytes with respect to the ratio of central to

268 Treatment of atrial myocytes with the GSK3beta inhibitor Kenpaullone

269 s activated a large outward current from rat atrial myocytes, with a parallel decrease in action pote