ライフサイエンスコーパス: autolysis

1 el and inner ear cells are very sensitive to autolysis.

2 XCP2 has a minor but distinct role in micro-autolysis.

3 r through trypsin-mediated cleavages, termed autolysis.

4 f AtlA, and the mutant was hyperresistant to autolysis.

5 els of AtlA protein and led to resistance to autolysis.

6 ytic cleavage site and protects MT1-MMP from autolysis.

7 system that is involved in the regulation of autolysis.

8 rected gene expression, cell separation, and autolysis.

9 d in cell separation, biofilm formation, and autolysis.

10 , biofilm formation, genetic competence, and autolysis.

11 s responsible for cell wall breakdown during autolysis.

12 s and is dependent on the intrinsic level of autolysis.

13 could be inhibited by the peptide product of autolysis.

14 nsitive to EDTA, a compound known to trigger autolysis.

15 of function of Pep27 or VncRS does not alter autolysis.

16 e virulence genes and more genes involved in autolysis.

17 owed reduced rates of cell wall turnover and autolysis.

18 that, when purified, it apparently underwent autolysis.

19 ctioning mitochondria by processes involving autolysis.

20 e regulators, involved in regulation of cell autolysis.

21 e loss of organelles as a result of cellular autolysis.

22 absence of active MMP-2, suggesting MT1-MMP autolysis.

23 ical content coevolved in proteases to avoid autolysis.

24 refore, it appeared to be an easy target for autolysis.

25 l enzymes that dissolve the cell wall during autolysis.

26 system affects murein hydrolase activity and autolysis.

27 a small but significant decrease in rates of autolysis.

28 analyze the changes in oligomerization upon autolysis.

29 is in the control pathway for triggering of autolysis.

30 umococcal physiology including adherence and autolysis.

31 rus migration through them, concomitant with autolysis.

32 lcium concentrations below that required for autolysis.

33 e cell wall synthesis machinery and triggers autolysis.

34 e associated only concomitant with bacterial autolysis.

35 in in protection against complement-mediated autolysis.

36 re, did not correlate with susceptibility to autolysis.

37 forms correlate with differences in rates of autolysis.

38 n1 correlated with a defect in LytA-mediated autolysis.

39 s was much lower than that for the classical autolysis.

40 ted with defects in cell shape and increased autolysis.

41 oxin pneumolysin through increased bacterial autolysis.

42 of extracellular LytA dictates the onset of autolysis.

43 ometry, which triggers the slower process of autolysis.

44 activity, and show increased sensitivity to autolysis.

45 on the autolytic sites of CAPN3, rather than autolysis.

46 hibition of cell wall synthesis, and trigger autolysis.

47 matic effect on murein hydrolase activity or autolysis.

48 ell growth under heat stress, cell division, autolysis, adherence and transformation.

49 essure, which is possibly related to reduced autolysis after exposure to subinhibitory concentrations

50 This strain was unique in showing decreased autolysis after growth in these conditions.

51 ith disintegrating cellular material as mega-autolysis, aided by additional lytic enzymes, destroyed

52 ependently down-regulated sarV (a marker for autolysis), although the alteration in sarV expression d

53 revealed that TiO2 NPs prevent or delay cell autolysis, an important survival and growth-regulating p

54 to be more resistant to Triton X-100-induced autolysis and also to lysis by lysostaphin.

55 ddition, the arlS mutant exhibited increased autolysis and altered peptidoglycan hydrolase activity c

56 The lytS mutant also exhibited increased autolysis and an altered level of murein hydrolase activ

57 toplasmic proteins are released by bacterial autolysis and become adsorbed to the surface of intact b

58 study, the effects of oxidation on calpain I autolysis and calpain-mediated proteolysis were examined

59 However, both autolysis and capsule shedding depend on the cell wall h

60 r postmortem developmental events, including autolysis and chromatin degradation.

61 potential mechanism of Gcp's involvement in autolysis and demonstrated that Gcp may function indepen

62 s membrane integrity, causing bacterial cell autolysis and DNA release.

63 In order to better understand autolysis and in hopes of creating a nonautolytic mutant

64 ant that exhibited a growth defect, enhanced autolysis and increased sensitivity to Triton X-100 and

65 ion prevented the mature enzyme from limited autolysis and irreversible inactivation.

66 late also had decreased Triton X-100-induced autolysis and killing when incubated in broth media cont

67 onomeric forms of MT6-MMP exhibited enhanced autolysis and metalloprotease-dependent degradation.

68 y, (i) an enzyme optimized for resistance to autolysis and oxidation, referred to as the cleavage-res

69 Post-mortem tissue analyses showed autolysis and retention of (210)Po at lethal doses in se

70 depends on the rates of cell wall synthesis, autolysis and the antimicrobial concentration.

71 GelE and SprE--is responsible for regulating autolysis and the release of high-molecular-weight eDNA,

72 ion of 19 potential target genes involved in autolysis and virulence, phenotypes affected by sarA and

73 in with apparent roles in biofilm formation, autolysis, and cell division.

74 differences such as faster growth, increased autolysis, and decreased intracellular hemolytic activit

75 lation of Stk1 function, hemolysin activity, autolysis, and GBS virulence.

76 isms, including specific secretion pathways, autolysis, and membrane vesicle formation.

77 ilm formation, fibronectin-binding capacity, autolysis, and protease and nuclease activities.

78 h, tendency to aggregate in culture, reduced autolysis, and reduced ability to grow under stress, inc

79 of calcium on the enzyme include activation, autolysis, and subunit dissociation.

80 echanism, but rather to bacterial leakage or autolysis, and that the extracellular abundance of these

81 iability following exponential growth due to autolysis, and the necessity for using high starting ino

82 aphylococcus aureus on cell wall metabolism, autolysis, and virulence, mostly in S. aureus laboratory

83 otonmotive-force and preventing the onset of autolysis; and (ii) indirectly, through adsorption of au

84 This study examined whether differences in autolysis are caused by differences in expression of the

85 beta-lactams were dramatically less prone to autolysis as a result of decreased transcription and enz

86 lysin LytA is a virulence factor involved in autolysis as well as in fratricidal- and penicillin-indu

87 asic mechanism of proteolysis and propeptide autolysis, as well as the evolutionary pressures that dr

88 Therefore, prior autolysis at 60 degrees C for 60 min increased the extra

89 e shrimp (Litopenaeus vannamei) subjected to autolysis at 60 degrees C for different times (0, 30, 60

90 For comparison, a classical yeast autolysis at mild temperature (25 degrees C) was perform

91 comparable, and the active enzymes suffered autolysis at similar rates, indicating that neither cata

92 ctive protein is readily inactivated through autolysis at specific internal arginine positions.

93 be determined, several factors influence the autolysis behavior of S. pneumoniae, including the bacte

94 s place in 3 distinct steps: (i) blockage of autolysis by reducing or anaerobic conditions, (ii) rapi

95 molecular-weight excreted molecule, triggers autolysis by self-perturbing the electron transfer react

96 1' position of this cleavage site attenuated autolysis by the enzyme and restored wild-type dimerizat

97 ia and has been conventionally attributed to autolysis by the LytA amidase.

98 o potential mechanisms for the disruption of autolysis by TiO2 NPs in a concentration dependent manne

99 Here we show that Ply released by autolysis cannot reassociate with intact cells, suggesti

100 heir growth, stress tolerances, respiration, autolysis, cell death, sterigmatocystin production, hyph

101 s involved in different functions, including autolysis, cell division, growth, and pathogenesis.

102 mutant underwent increased stationary-phase autolysis compared to the parental strain.

103 competitive oral biofilm environment, where autolysis could create open spaces for competitors to in

104 The autolysis deficient DeltalytA mutant and its isogenic ch

105 Isogenic wild-type (lyt+) and autolysis-deficient (lyt-) strains of S. aureus were equ

106 g an autolytic activity was identified in an autolysis-deficient mutant (Lyt-) of Staphylococcus aure

107 gether, these data showed that ArlRS impacts autolysis differently in MSSA and MRSA strains.

108 ometry indicate that the factor Xa underwent autolysis during crystallization and the first EGF-like

109 est that H. pylori cells undergo spontaneous autolysis during culture and that urease and HspB become

110 The role of autolysis during gonococcal infection is not known, but

111 r tools for the dissection of the process of autolysis during xylogenesis, and for the dissection of

112 ne and serine proteases might be involved in autolysis during xylogenesis.

113 In contrast to classical bacterial autolysis, during capsule shedding, LytA promotes bacter

114 Autolysis, eDNA release, and atlS expression increased s

115 appeared to be more resistant to postmortem autolysis effects.

116 Under conditions that prevent autolysis, embryos within the fertilization envelope can

117 cassette, and the mutants were analyzed for autolysis, extracellular DNA release, biofilm formation,

118 the ability to form disulfide bonds affected autolysis, extracellular DNA release, biofilm formation,

119 s release of the enzyme from bacteria due to autolysis followed by adsorption of the enzyme to the su

120 ing mitochondrial import, but was removed by autolysis following calpain activation.

121 ta-lactone resulted in partial uncoupling of autolysis from differentiation.

122 e also revealed that beta-lactams modify the autolysis function (the natural process of self-exfoliat

123 homogenization (HPH) was tested for inducing autolysis in a commercial strain of Saccharomyces bayanu

124 Ca(2+), Asn-21- and Ile-21-trypsins suffered autolysis in an indistinguishable manner, whereas Thr-21

125 The disruption of autolysis in B. subtilis cultures by TiO2 NPs suggests t

126 eath is a result of loss in cell wall due to autolysis in combination with stinted replenishing.

127 ctam resistance or in the regulation of cell autolysis in E. hirae.

128 It would also induce autolysis in many Gram-positive species, thereby releasi

129 m that has been shown to negatively regulate autolysis in methicillin-sensitive Staphylococcus aureus

130 Lower levels of autolysis in opaque variants, however, was associated wi

131 appears unaffected by conditions that cause autolysis in other eDNA-producing bacteria.

132 ys that control twitching motility, TTSS and autolysis in P. aeruginosa.

133 eB-null mutant exhibits an increased rate of autolysis in response to cell wall-targeting antibiotics

134 S and lytR gene products control the rate of autolysis in S. aureus by affecting the intrinsic murein

135 by which mgrA and sarA gene products control autolysis in S. aureus.

136 n calpain-mediated proteolysis and calpain I autolysis in situ were examined.

137 usly identified mgrA (rat) as a regulator of autolysis in Staphylococcus aureus.

138 However, amiC mutants still underwent autolysis in stationary phase, indicating that other gon

139 y is presented, as are the possible roles of autolysis in the viral replication cycle.

140 arlRS mutant via a multicopy plasmid induced autolysis in this MRSA strain.

141 of cell wall turnover and detergent-induced autolysis in virtual parallel with the increasing MIC fo

142 gest that agr and sar exert their effects on autolysis, in part, by modulating murein hydrolase expre

143 ed wild-type sensitivity to other classes of autolysis-inducing antibiotics.

144 ed the cell survival of Bacillus subtilis in autolysis-inducing buffer by 0.5 to 5 orders of magnitud

145 An intrinsic substrate of MTG is the dispase autolysis-inducing protein (DAIP).

146 eased enzymes, supporting both mechanisms of autolysis interference.

147 lactams, suggesting that HQNO-dependent cell autolysis is advantageous to the bacterial populations.

148 ng Newman, SH1000, RN6390, and 8325-4, where autolysis is affected by ArlRS.

149 nsitive to protein dilution, confirming that autolysis is intramolecular.

150 Membrane-dependent autolysis is markedly reduced for fXSt. Louis II.

151 ar DNA readily, suggesting that partial cell autolysis is not required for DNA degradation.

152 The dynamic equilibrium between growth and autolysis is perturbed by the presence of the antimicrob

153 Release of DNA without detectable autolysis is suggested to be an adaptation to the compet

154 Concomitant with autolysis is the generation of an N-terminally truncated

155 ivated proteases in post-necrotic myocardial autolysis is well characterized, their importance in hom

156 the surface of intact bacteria ("altruistic autolysis"), is essential for survival of H. pylori in a

157 The terminal process of xylogenesis, autolysis, is essential for the formulation of a tubular

158 Thus, HQNO-driven autolysis links programmed cell death with quorum sensin

159 ed enzyme: the N-terminus (Gly14-Gly19), the autolysis loop (Gly142-Thr154), and the 180s loop (Pro18

160 The autolysis loop (residues 143-154 in chymotrypsinogen num

161 ognition site for interaction with the basic autolysis loop (residues 143-154) of fXa.

162 Because the autolysis loop affects fibrinogen binding, but not prote

163 ed that Arg-143, Lys-147, and Arg-154 of the autolysis loop and Lys-96, Lys-169, and Lys-236 of the h

164 Conversely, the autolysis loop and sodium-binding site exchanged more re

165 Modeling indicated steric proximity of the autolysis loop and the activation peptide in trypsinogen

166 f fXa that 1) contained substitutions in the autolysis loop and the heparin binding exosite, 2) lacke

167 n additional cleavage site at Leu-148 in the autolysis loop and the lack of the conserved Cys-139-Cys

168 esults suggest that Arg143 and Lys147 of the autolysis loop are recognition sites for FX independent

169 arkable feature of the structure is that the autolysis loop assumes a helical conformation enabling W

170 croM), IXaE245V 225 microM (240 microM), and autolysis loop cleaved IXaE245V 330 microM (350 microM).

171 To investigate the role of this autolysis loop in fX function, a recombinant variant wit

172 bition of autoactivation via cleavage of the autolysis loop is the dominant mechanism that can mitiga

173 e autolysis loop; and (d) proteolysis in the autolysis loop leads to a loss of catalytic efficiency w

174 ptide in trypsinogen, suggesting the cleaved autolysis loop may directly interfere with activation.

175 These results suggest that Arg(150) of the autolysis loop may specifically interact with the activa

176 The autolysis loop occupies a position up to 10 A closer to

177 The so-called autolysis loop of APC (residues 301-316, equivalent to c

178 zed that differences in the structure of the autolysis loop of coagulation proteases (residues 143-15

179 s suggest that structural differences in the autolysis loop of coagulation proteases play a key role

180 ge at Arg-318-Ser-319 in the protease domain autolysis loop of factor IXa results in its diminished b

181 The autolysis loop of factor Xa (fXa) has four basic residue

182 Previously, we identified Arg(150) on the autolysis loop of FXa as a candidate residue that may sp

183 ts demonstrate that basic amino acids in the autolysis loop of fXIa are important determinants of ser

184 rg318-Ser319[150-151] in the protease domain autolysis loop of IXaE245V with a concomitant loss of co

185 aved the Phe-150-Gly-151 peptide bond in the autolysis loop of T8 and T9 and inhibited autoactivation

186 g-144, Lys-145, Arg-147, and Lys-149) in the autolysis loop of the coagulation protease factor XIa (f

187 The autolysis loop of thrombin comprises nine residues, from

188 shown that deletion of nine residues in the autolysis loop of thrombin produces a mutant with an ant

189 to thrombin-ABE I ligand complexes with the autolysis loop often most affected.

190 6A, 311A, 312A, and 314A) suggested that the autolysis loop provides for up to 15-fold discrimination

191 sumes an unprecedented conformation with the autolysis loop shifted 20 Angstroms away from its canoni

192 Des-44-Xa in which the autolysis loop was cleaved possessed </=5% of the amidol

193 Phe137 in euphauserase, localized in loop D (autolysis loop), is highly exposed on the surface of the

194 wo distinct exosites of fXa (36-loop and the autolysis loop).

195 tion of rTAP with the P2-binding pocket, the autolysis loop, and the Na(+)-binding loop is primarily

196 e, the Na(+) binding loop, the 186-loop, the autolysis loop, exosite I, and exosite II.

197 ected regions include segments of ABE-I, the autolysis loop, the edge of the active site region, and

198 mbined with deletion of nine residues in the autolysis loop, which by itself shifts the specificity o

199 The autolysis loop, which is disordered in the uninhibited f

200 .26 nM); IXaE245V, 2.5 microM (1.35 nM); and autolysis loop-cleaved IXaE245V, 15.6 microM (14.3 nM).

201 Additionally, autolysis loop-cleaved, active site-blocked native facto

202 he backbone away from exosite I and over the autolysis loop.

203 -332-Gln-333(150-151) in the protease domain autolysis loop.

204 e of the Leu-149-Ser-150 peptide bond in the autolysis loop.

205 ately 3-fold and prevents proteolysis in the autolysis loop; and (d) proteolysis in the autolysis loo

206 The autolysis loops (amino acids 143-154, chymotrypsinogen n

207 The zymogen positioning of both the 180s and autolysis loops are synergistic structural elements that

208 To test this hypothesis, the autolysis loops of both thrombin and the anticoagulant s

209 The mechanism of autolysis may involve activation of the endogenous proph

210 elements differentiate through a specialized autolysis mechanism.

211 This is consistent with our altruistic autolysis model in which H. pylori uses genetically prog

212 lysis of a calpain substrate alpha-spectrin, autolysis of activated calpain, and reduction of cell da

213 ish fillets was carried out by assessment of autolysis of cells using a cytosolic enzyme lactate dehy

214 Autolysis of H. pylori is an important phenomenon to rec

215 However, autolysis of human cationic trypsin is very slow in vitr

216 the MurA protein is involved in generalized autolysis of L. monocytogenes.

217 nactivation of arlRS does not play a role in autolysis of methicillin-resistant S. aureus (MRSA) stra

218 by crystallography and NMR spectra, prevents autolysis of MMP-12 and allows us to determine its NMR s

219 lood culture bottles may be difficult due to autolysis of pneumococci.

220 during high pressure homogenization-induced autolysis of Saccharomyces bayanus wine yeasts, treated

221 e importance of mgrA and sarA in controlling autolysis of Staphylococcus aureus, with MgrA and SarA b

222 ntration was greater than 40 microM, whereas autolysis of the 30-kDa subunit did not occur until the

223 Autopsy revealed autolysis of the kidneys and submassive bridging necrosi

224 has been suggested to play a pivotal role in autolysis of the parasitic cell wall of Coccidioides imm

225 disulfide interchange, transpeptidation, and autolysis of the protease itself may occur.

226 proteolytic fragments (including those from autolysis of the protease) can be severe, due to high pr

227 strated that NEG can be generated during the autolysis of the yeast used in the preparation of the ye

228 The autolysis of the ypfP::cat mutant in the presence of 0.0

229 the beta-lactam resistance, growth, and cell autolysis of wild-type strain ATCC 9790 and resistant st

230 PH seemed a promising technique for inducing autolysis of wine yeasts.

231 Establishing the mechanisms regulating the autolysis of xylem tracheary elements (TEs) is important

232 its inactive form, intermolecular cleavage (autolysis) of AtMCP2d could also occur under our assay c

233 uring gonococci by two different mechanisms: autolysis or type IV secretion.

234 ly has been hypothesized to be released upon autolysis or, alternatively, via a nonautolytic mechanis

235 ly has been hypothesized to be released upon autolysis or, alternatively, via a nonautolytic mechanis

236 At 20 microM Ca2+ many vesicles underwent autolysis, or were so weak that they burst instantly on

237 ent were observed within the first 60 min of autolysis (p>0.05), but subsequently increased up to 150

238 f nonapoptotic processes (necrosis, in vitro autolysis, peroxide damage, and heating) single-base 3'

239 ctionality (via delta-lysin production), and autolysis phenotypes were assessed in MRSA isolates from

240 Autolysis plays an essential role in bacterial cell divi

241 Autolysis plays an important role in spoilage of fish an

242 of the catalytically inactive 43-kDa MT1-MMP autolysis product and decline in the TIMP-2 levels in co

243 hat 3,3'-diindolylmethane (DIM), a vegetable autolysis product, promoted Fas-mediated apoptosis of ch

244 e coverage up to 90% within minutes; trypsin autolysis products are not detected.

245 e apparent molecular weights as the in vitro autolysis products.

246 pression did not correlate directly with the autolysis profiles of single mgrA and sarA mutants.

247 XCP1 could be involved in tracheary element autolysis, promoter activity and localization of XCP1 we

248 motif did not prevent autoactivation but the autolysis rate was somewhat reduced.

249 yer, resulting in a dramatic decrease of the autolysis rate.

250 olysis, resulting in decreased and increased autolysis rates, respectively.

251 y to benzylpenicillin or the growth and cell autolysis rates.

252 mutants in many aspects including increased autolysis, reduced levels of surface-assembled tfp and d

253 g the most profoundly upregulated genes were autolysis-related genes and those that encode bacterioci

254 two TEV protease mutants, inactive C151A and autolysis-resistant S219D, have now been solved at 2.2-

255 Several hydrolase types are implicated in autolysis responsible for the breakdown of cytoplasm.

256 factor regulatory genes, agr and sar, affect autolysis, resulting in decreased and increased autolysi

257 completely inhibited cell wall turnover and autolysis, resulting in the accumulation of cell wall ma

258 he design of an enzyme variant stabilized to autolysis should further the structural and mechanistic

259 MSP may be regulated in part by autolysis, since the active protein is readily inactivat

260 In addition, we identified an autolysis site between residues 95e and 95f in the 99-lo

261 g the herpesvirus proteases in possessing an autolysis site in the dimer interface, which removes the

262 An autolysis site of functional and structural significance

263 The autolysis site of the enzyme is separated from its catal

264 Four primary PR autolysis sites were blocked via substitution of either

265 se determined that blocking all four primary autolysis sites yielded a cleavage-resistant PR which wa

266 The fact that PQS levels correlated with autolysis suggests a fine balance in natural populations

267 would have a higher energy of activation for autolysis than chains aligned circumferentially.

268 rations (eg, thicker cell walls and abnormal autolysis) that are typical of in vivo VISA mutants.

269 , Pseudomonas aeruginosa is shown to control autolysis through the production of HQNO, a quorum-sensi

270 otenoid content was obtained with increasing autolysis time (p<0.05).

271 An autolysis time course determined that blocking all four

272 ne value (AV) of lipids were noticeable when autolysis time increased (p<0.05).

273 raction yield increased from 7.4% to 8.8% as autolysis time increased from 0 to 150 min.

274 distribution were not linked to age, gender, autolysis time, or subtype of schizophrenia.

275 hrenic subjects and their age-, gender-, and autolysis time-matched control subjects.

276 pylori uses genetically programmed bacterial autolysis to release urease and other cytoplasmic protei

277 we noted that it underwent autoproteolysis (autolysis) to give discrete cleavage products.

278 Unlike the classical autolysis, ultrasound led to a high cell disruption, and

279 Neisseria gonorrhoeae is prone to undergo autolysis under many conditions not conducive to growth.

280 grA is a pleiotropic regulator that controls autolysis, virulence, and efflux pump activity in Staphy

281 Autolysis was also postulated to play a protective role

282 ivity suggested that the observed incomplete autolysis was due to the ability of LLL to inhibit TE cy

283 Significant autolysis was induced in the Synechocystis sp. PCC 6803

284 ithin the intact central vacuole before mega-autolysis was initiated by tonoplast implosion.

285 ative stress; however, the extent of calpain autolysis was not altered.

286 pneumococci during log-phase growth, because autolysis was not believed to occur at this time.

287 than in the wild type) after the final mega-autolysis was otherwise complete.

288 biosynthesis, and in one suppressed mutant, autolysis was restored by addition of synthetic PQS.

289 Autolysis was suppressed by mutation of genes required f

290 Ser 4 His to stabilize the protease against autolysis) was determined to 2.0 A resolution in a new s

291 The rates of VacA secretion and autolysis were each influenced by medium composition, an

292 hepatopancreas without and with 60 min prior autolysis were observed.

293 activation, zymogen degradation, and trypsin autolysis were studied.

294 on of rpoN rendered E. faecalis resistant to autolysis, which in turn impaired eDNA release.

295 th, we examined the role of Gcp in bacterial autolysis, which is an important biological process for

296 EASE1 (XCP1) and XCP2, participated in micro-autolysis within the intact central vacuole before mega-

297 ons did result in a significant reduction of autolysis without altering calpain proteolytic activity.