ライフサイエンスコーパス: auxotroph

1 lds a strain that is a stringent lyso-PtdEtn auxotroph.

2 unlike many microorganisms, was a glutamate auxotroph.

3 desaturate exogenous stearate and was an UFA auxotroph.

4 Chlamydia pneumoniae is a natural tryptophan auxotroph.

5 defunct glycolytic pathway of the bacterial auxotroph.

6 ed from a normally avirulent p-aminobenzoate auxotroph.

7 viously to support the growth of an inositol auxotroph.

8 roven by the reduced virulence of a cysteine auxotroph.

9 ds, but the lysC strain is not an amino acid auxotroph.

10 ior of Caenorhabditis elegans, a cholesterol auxotroph.

11 of SHAM-sensitive respiration of the E. coli auxotroph.

12 ied by complementation of a bacterial purine auxotroph.

13 ontain an insertion in ura3 and was a uracil auxotroph.

14 ngs demonstrate that P. carinii is an AdoMet auxotroph.

15 ional complementation of an Escherichia coli auxotroph.

16 , and shown to support growth of a cobalamin auxotroph.

17 One mutant, JJ104, was a leaky acetate auxotroph.

18 demethylation of lanosterol and is itself an auxotroph.

19 plsX double knockout strain was a fatty acid auxotroph.

20 pdxR mutant also exhibited as a vitamin B(6) auxotroph.

21 through expression in an E. coli methionine auxotroph.

22 es for C. trachomatis, which is a tryptophan auxotroph.

23 owth arrest, an unusual phenotype for an Mtb auxotroph.

24 tation, suggesting that blk1-R is a thiamine auxotroph.

25 nd met31 met32 double mutants are methionine auxotroph.

26 ssue by using Salmonella typhimurium leu-arg auxotrophs.

27 icted media, confirming them as his-negative auxotrophs.

28 absence of large collections of informative auxotrophs.

29 oteins, which rescued four different E. coli auxotrophs.

30 resulted in growth inhibition of lyso-PtdEtn auxotrophs.

31 ic mutants, including amino acid and vitamin auxotrophs.

32 than half of the algal kingdom are cobalamin auxotrophs.

33 tryptophan (7AW), using Escherichia coli Trp auxotrophs.

34 t the growth of the tryptophan and histidine auxotrophs.

35 ire1Delta and hac1Delta mutants are inositol auxotrophs.

36 pe3Delta, and spe4Delta are all beta-alanine auxotrophs.

37 h genes, however, are unsaturated fatty acid auxotrophs.

38 onal complementation of Escherichia coli His auxotrophs.

39 cleave SREBPs at Site-2 and are cholesterol auxotrophs.

40 mutants; these strains are therefore glucose auxotrophs.

41 bA2(Ts) or fabB15(Ts) unsaturated fatty acid auxotrophs.

42 a useful method for screening for polyamine auxotrophs.

43 r vaccine strategies using engineered uracil auxotrophs.

44 Chlamydia and Leishmania, natural tryptophan auxotrophs.

45 an adequate purine source for gastric purine auxotrophs.

46 f triple-negative samples that are glutamine auxotrophs.

47 depend on oxidative metabolism but are heme auxotrophs.

48 enterica yhhK strains are also pantothenate auxotrophs.

49 As predicted, DeltagcvH strains are lipoate auxotrophs.

50 Met31p and Met32p respond poorly, like other auxotrophs.

51 age pathways of Leishmania, which are purine auxotrophs.

52 ncorporated into proteins using L-methionine auxotrophs.

53 orting the growth of S. cerevisiae polyamine auxotrophs.

54 (iii) In derepressed auxotrophs, 100% of the codons containing the mutable ba

55 , hac1Delta and ire1Delta, are also inositol auxotrophs, a phenotype associated with defects in expre

56 We generated a diaminopimelic acid (DAP) auxotroph (AA400) of L. pneumophila by disruption of the

57 no longer be detected in mice receiving the auxotrophs after 16-32 weeks, and that infected SCID mic

58 The citB2 mutant was a glutamate auxotroph and accumulated citrate, while the citB7 mutan

59 l complementation of an Escherichia coli Tyr auxotroph and by enzyme assays.

60 ince Enterococcus faecalis is a natural heme auxotroph and cause of bloodstream infection, we explore

61 ion, both mutants complement an E. coli OSBS auxotroph and have measurable levels of OSBS activity.

62 ound that an ade13Delta mutant is an adenine auxotroph and is unable to successfully cause infections

63 a nicotinamide adenine dinucleotide (NAD(+)) auxotroph and its growth depends on the environmental su

64 an parasite Leishmania amazonensis is a heme auxotroph and must acquire this essential factor from th

65 L. monocytogenes is a lipoate auxotroph and must scavenge this critical cofactor, usin

66 Caenorhabditis elegans is a heme auxotroph and relies solely on environmental heme for su

67 d the resulting strain was both a fatty acid auxotroph and required de novo fatty acid synthesis for

68 thesis, which converts Mtb into a tryptophan auxotroph and restores the efficacy of a failed host def

69 sis challenge can be achieved with a leucine auxotroph and suggest that to induce optimal protection,

70 A BR2.024 dapB mutant is a diaminopimelate auxotroph and tabtoxin negative.

71 A Schu S4 DeltapanG strain is a pantothenate auxotroph and was genetically and chemically complemente

72 w, on average, 83% overlap between unrelated auxotrophs and 35% with previously published transcripto

73 for our studies because they are cholesterol auxotrophs and can be depleted of cholesterol by growth

74 paired mitochondrial function become uridine auxotrophs and can be maintained with high micromolar co

75 vaccine by constructing a lineage of guanine auxotrophs and conducted a double-blind, placebo-control

76 rnate host for alphaviruses, are cholesterol auxotrophs and contain very low levels of this sterol.

77 which the promoter is highly methylated are auxotrophs and express ASY at very low levels.

78 These mutants are choline auxotrophs and fall into two unlinked complementation gr

79 screen in Mycobacterium smegmatis for biotin auxotrophs and identified pyruvate carboxylase (Pyc) as

80 e ease with which we were able to screen for auxotrophs and mutants with pigmentation and capsular ph

81 the prevailing laboratory practice of using auxotrophs and nutrient supplemented media, these result

82 p1 mutants consist of two groups, tryptophan auxotrophs and prototrophs, that differ significantly in

83 firmed that zwf1Delta mutants are methionine auxotrophs and report that they also are oxygen-sensitiv

84 susceptible BALB/c mice were immunized with auxotrophs and subsequently challenged with virulent Myc

85 the conclusion that AM fungi are fatty acid auxotrophs and that plants provide their fungal endosymb

86 The resulting strain, CSDX1, was a glutamate auxotroph, and enzyme assays confirmed the absence of a

87 C. glabrata is a NAD(+) auxotroph, and its growth depends on the availability of

88 lciparum (PfADA) is essential in this purine auxotroph, and its inhibition is expected to have therap

89 C. glabrata is an NA auxotroph, and NA-induced EPA expression is likely the r

90 e ino1Delta/ino1Delta mutant was an inositol auxotroph, and the itr1Delta/itr1Delta mutant was unable

91 de conditional lethal mutations, conditional auxotrophs, and conditional mutants of the eut (ethanola

92 are viable, are not threonine or methionine auxotrophs, and express wild-type levels of aspartokinas

93 These strains are uracil auxotrophs, and their growth was attenuated on cysteine

94 t to mycorrhizal fungi, which are fatty acid auxotrophs, and this lipid export requires the glycerol-

95 Here we show that T. gondii uracil auxotrophs are completely avirulent not only in immune-c

96 We have characterized one of these auxotrophs as being disrupted in cysA.

97 The data for all auxotrophs at all GRs recapitulated the features of aero

98 growth, and by limiting its availability to auxotrophs, B12-producing organisms may facilitate coord

99 The sucA mutant is not a succinate auxotroph but has a reduced ability to use glutamate as

100 ccumulate in tryptophan, adenine, and uracil auxotrophs but not in prototrophic strains, indicating t

101 ions are induced during starvation of a hemA auxotroph, but the level of induction observed was maxim

102 aD) mutant was confirmed to be a strict B(6) auxotroph, but the pdxT (yaaE) mutant turned out to be a

103 dicated that gdhA mutants were not glutamate auxotrophs, but attempts to isolate similar mutants with

104 n that E. coli yhhK strains are pantothenate auxotrophs, but the role of YhhK in pantothenate biosynt

105 rst, growth complementation of an L-arginine auxotroph by D-arginine was abolished by a lesion at dau

106 Subcutaneous immunization with this auxotroph conferred protection in C57BL/6J mice against

107 smegmatis afforded a cysteine and methionine auxotroph consistent with a metabolic branch point cente

108 strain were isolated that were octanoic acid auxotrophs consistent with biochemical studies indicatin

109 iously shown that a P. aeruginosa tryptophan auxotroph could grow on tryptophan-depleted medium with

110 nation with the nonreplicating type I uracil auxotroph cps1-1 strain.

111 able to complement their respective E. coli auxotrophs, demonstrating that they encoded functional e

112 We constructed synthetic auxotrophs dependent on sAAs that were not rescued by cr

113 (yaaE) mutant turned out to be a conditional auxotroph depending on the availability of ammonium in t

114 The survival rates of nongrowing auxotrophs deprived of uracil or leucine depended on the

115 I results in an unsaturated fatty acid (UFA) auxotroph despite the presence of fabK, a gene encoding

116 This apparently simple auxotroph did not grow well in rich media containing exc

117 gle-amino-acid starvation, whereas a proline auxotroph did.

118 Tryptophan and histidine auxotrophs did not survive single-amino-acid starvation,

119 rates of two isogenic Escherichia coli K-12 auxotrophs differing only in relA have been determined i

120 meshifting was also prevalent in a polyamine auxotroph double mutant, a polyamine-independent regulat

121 tant strain was also found to be an inositol auxotroph due at least in part to an inability to proper

122 wth of E. coli strain BB26-36 which is a G3P auxotroph due to an altered G3P acyltransferase activity

123 epatocellular carcinoma (HCC) is an arginine auxotroph due to argininosuccinate synthetase I deficien

124 -/-) mice with an avirulent T. gondii uracil auxotroph elicited robust IFN-gamma responses and protec

125 Since humans are ascorbate auxotrophs, enhancing the nutritional quality of a widel

126 we demonstrate that treatment of the sterol auxotrophs erg25 slu1 or erg25 slu2 with azole antibioti

127 An ergosterol auxotroph, erg25, which fails to demethylate and concomi

128 ituted protein was induced in the methionine auxotroph, Escherichia coli DL41DE3, purified, and cryst

129 t signaling by growing, in chemostats, yeast auxotrophs for histidine, lysine, or uracil in excess of

130 Mutants lacking both of these enzymes were auxotrophs for isoleucine, whereas single mutants were c

131 Cells of the immune system are auxotrophs for most amino acids, including several nones

132 Many algae are auxotrophs for vitamin B(12) (cobalamin), which they nee

133 With the exception of heme auxotrophs, gentamicin-resistant SCVs displayed greater

134 medium-copy levels of abgAB, p-aminobenzoate auxotrophs grew on 50 nM p-aminobenzoyl-glutamate.

135 Two additional auxotrophs grew poorly in the absence of acetate but con

136 Six of the auxotrophs had an absolute growth requirement for acetat

137 In these sulfur auxotrophs, heavy sulfate is channeled exclusively into

138 gen, Entamoeba histolytica, is a nucleo-base auxotroph (i.e. lacks the ability to synthesize purines

139 er trypanosomatid parasites, they are purine auxotrophs (i.e. lack the ability to synthesize purines

140 genomic library in an E. coli DeltadapD DAP auxotroph identified ct390 as encoding an enzyme that re

141 ave a similar phenotype to the bio1 and bio2 auxotrophs identified using forward genetic screens for

142 test of the vaccine potential of the leucine auxotroph, immunocompetent BALB/c mice, susceptible to f

143 d by expressing the proteins in a methionine auxotroph in the presence of l-methionine with the side

144 the limited collection of well-characterized auxotrophs in flowering plants.

145 Growth of the auxotrophs in medium containing [(14)C]acetate yielded (

146 Psd1p that relies on isolating ethanolamine auxotrophs in suitable (psd2Delta) genetic backgrounds.

147 rocedure was developed for the enrichment of auxotrophs in the antibiotic-insensitive archaebacterium

148 Their results were obtained with a leucine auxotroph; in leucine prototrophs grown in a medium lack

149 ruption of ask was also possible in a lysine auxotroph incapable of converting DAP to lysine.

150 t of therapeutic agents in humans and purine auxotrophs, including malarial parasites.

151 Several classes of methionine auxotrophs, including metX and metW mutants, exhibit red

152 purine base salvage in humans and in purine auxotrophs, including Plasmodium falciparum, the leading

153 Strains lacking FabB are UFA auxotrophs indicating that the enzyme catalyzes an essen

154 Exploiting the ability of the inositol auxotroph, ino1, to use exogenous GroPIns as an inositol

155 A single injection of the uracil auxotroph into BALB/c mice induces long-term protective

156 The deltaleuD deltapanCD double auxotroph is fully attenuated in the SCID mouse model an

157 criptional growth-rate response (GRR) in the auxotrophs is only 40-50% of the magnitude in prototroph

158 A second acetate auxotroph, JJ117, had an absolute growth requirement for

159 Members of the Nematoda are heme auxotrophs, lacking the ability to synthesize heme; howe

160 ases to the growth of the 14-amino-acid (aa) auxotroph Lactobacillus helveticus CNRZ32, single- and m

161 Similar comparisons using leucine or uracil auxotrophs limited on leucine or uracil again showed pat

162 sed for S1P because all previous cholesterol auxotrophs manifest defects in S2P, which is encoded by

163 f E. faecalis in mice; the attenuated purine auxotroph may provide a system for developing vectors fo

164 unencapsulated, and para-amino benzoic acid auxotroph mutant TIGR4 strains to assess the effects of

165 and it pinpoints weaknesses of commonly used auxotroph mutants for investigating metabolism.

166 ion of fungal proteomes, which often require auxotroph mutants to fully metabolically label.

167 trains, such as type I nonreplicating uracil auxotroph mutants, are highly effective in eliciting lif

168 . < or =10 ng/ml), we have generated acetate auxotrophs NMBACE1 from encapsulated Neisseria meningiti

169 plementation of two C. nicotianae pyridoxine auxotrophs not mutant in PDX1.

170 i -100 mV; a respiration-deficient menadione auxotroph of 6850) were used to assess the influence of

171 er and transformed into a D-Ala-D-Ala ligase auxotroph of Escherichia coli possessing deletions of bo

172 ed by functional complementation of a purine auxotroph of Escherichia coli that also harbors deficien

173 A leucine auxotroph of M. tuberculosis was created by allelic exch

174 genicity of a double lysine and pantothenate auxotroph of Mycobacterium tuberculosis in mice.

175 In this study, we used an acetate auxotroph of Neisseria meningitidis serogroup B to facil

176 chC expression is greatly repressed in a B12 auxotroph of Rhodobacter capsulatus and that B12 regulat

177 It is known that auxotrophs of a variety of bacteria are attenuated in vi

178 e the results of a systematic screen for His auxotrophs of Arabidopsis (Arabidopsis thaliana).

179 d S167C complemented the growth of thymidine auxotrophs of E. coli in medium lacking thymidine.

180 s confirmed by complementing the orthologous auxotrophs of Escherichia coli (bioD and bioA).

181 Tryptophan auxotrophs of Escherichia coli in which mutations were m

182 measured autoinducer synthesis by amino acid auxotrophs of Escherichia coli that contained luxI on a

183 Riboflavin, pantothenate, and biotin auxotrophs of Histoplasma were generated to probe whethe

184 Screening for amino acid auxotrophs of Mycobacterium bovis BCG, obtained by trans

185 Complementary auxotrophs of Saccharomyces cerevisiae amino acid and nu

186 Transformation of tryptophan auxotrophs of Streptomyces coelicolor A3(2) and subseque

187 screened a number of independent pyrimidine auxotrophs of Sulfolobus acidocaldarius for deletions an

188 Uracil auxotrophs of two H. capsulatum restriction fragment len

189 otein enabling the growth of p-aminobenzoate auxotrophs on exogenous p-aminobenzoyl-glutamate.

190 sed transpositional mutagenesis of a proline auxotroph (PAO951) to isolate an ornithine utilization (

191 as vaginalis is a parasitic protozoan purine auxotroph possessing a unique purine salvage pathway con

192 t Mycobacterium tuberculosis, several of the auxotrophs produced comparable protection against intrav

193 Two spontaneous revertants of these latter auxotrophs regained the ability to grow normally in the

194 The M. tuberculosis lysine auxotroph requires 25-fold more lysine on defined medium

195 Plasmodium falciparum is a purine auxotroph requiring hypoxanthine as a key metabolic prec

196 n mutagenesis, was used to select methionine auxotrophs requiring a sulphur-containing amino acid for

197 nd the structural requirements necessary for auxotroph rescue, we investigated the biophysical proper

198 n menD or hemB, yielding menadione and hemin auxotrophs, respectively, and studied in the rabbit endo

199 ng from Chlamydia Thus, how Chlamydia, a Trp auxotroph, responds to Trp starvation in the absence of

200 his study, we constructed similar tryptophan auxotroph revertants and found that the reversion result

201 Plasmodium falciparum is a purine auxotroph, salvaging purines from erythrocytes for synth

202 Unlike the wild-type, both pyrimidine auxotrophs secreted substantial quantities of orotate, s

203 MIs for 16 mutable bases in auxotrophs, selected during starvation in derepressed ge

204 Studies with sterol auxotrophs show that, even in the presence of sterols wh

205 use carA mutants are arginine and pyrimidine auxotrophs, single-site insertions at the glmS attTn7 si

206 nt for the growth of S. cerevisiae polyamine auxotrophs (spe1Delta, spe2Delta, and spe3Delta).

207 he live, attenuated Toxoplasma gondii uracil auxotroph strain cps1-1 induces long-lasting immunity ag

208 ing cps, an avirulent, nonreplicating uracil auxotroph strain of the parasite Toxoplasma gondii (T. g

209 The biotin auxotroph strain S288c was able to grow on media lacking

210 develop an avirulent nonreverting pyrimidine auxotroph strain.

211 in protein half-lives between prototroph and auxotroph strains.

212 n by detecting infection with the riboflavin auxotroph Streptococcus pyogenes.

213 deletion of ORF Vng1577 was not a cobalamin auxotroph, suggesting that either there is redundancy of

214 ant was constructed and found to be a lysine auxotroph, suggesting that this DapL homolog in methanoc

215 SCID mice infected with the pantothenate auxotroph survived significantly longer (250 days) than

216 All three auxotrophs survived complete starvation.

217 trate that erythrocytes from these ascorbate auxotrophs switch the preference of their glucose transp

218 h selection, using an unsaturated fatty acid auxotroph system, led to the isolation of variants with

219 lanosterol synthase mutant provided a sterol auxotroph that could be genetically complemented with th

220 e LVSDeltaguaA, an F. tularensis LVS guanine auxotroph that fails to replicate in the absence of exog

221 The nudA mutant was an auxotroph that grew slowly in liquid and on solid media

222 l complementation of an Escherichia coli Ile auxotroph that has been engineered to express ACC deamin

223 e roundworm Caenorhabditis elegans is a heme auxotroph that requires the coordinated actions of HRG-1

224 cal mutagenesis, we isolated an ethanolamine auxotroph that we designate pstA1-1.

225 egans and related helminths are natural haem auxotrophs that acquire environmental haem for incorpora

226 s not secreted by SRD-12B cells, cholesterol auxotrophs that lack S1P.

227 African trypanosomes are lipid auxotrophs that live in the bloodstream of their human a

228 C and DeltaOMPDC DeltaUP strains were uracil auxotrophs that rapidly lost their viability during pyri

229 MP synthase, C. neoformans becomes a guanine auxotroph, the production of key virulence factors is co

230 Some methionine auxotrophs (those with blocks in the biosynthetic pathwa

231 ions also reduced the growth of this E. coli auxotroph to about 85% of the control cells containing w

232 ts suggest that the inability of the leucine auxotroph to grow in mice was due to its sequestration,

233 was devised with the use of the E. coli UFA auxotroph to isolate mutants of the castor delta(9)-18:0

234 e URA3 gene efficiently transformed the ura3 auxotroph to prototrophy.

235 ked the viability of an E. coli L-methionine auxotroph to the activity of the improved enzyme.

236 The ability of Mycobacterium tuberculosis auxotrophs to survive long-term starvation was measured.

237 ck carbamoyl phosphate synthetase II (uracil auxotrophs) to determine whether de novo pyrimidine bios

238 opy-number plasmid pACYC184; p-aminobenzoate auxotrophs transformed with the clone encoding abgAB dem

239 tarvation for leucine in an Escherichia coli auxotroph triggers metabolic activities that specificall

240 A new ergosterol auxotroph unable to grow on 3-ketosterols without the ad

241 An ergosterol auxotroph unable to synthesize sterol or grow without st

242 luciliae, a primitive trypanosomatid, purine auxotroph, up-expressed its unique, bi-functional, surfa

243 We measured the survival of yeast auxotrophs upon starvation for different nutrients and f

244 A live, nonreplicating avirulent uracil auxotroph vaccine strain (cps) of Toxoplasma triggers no

245 ting, nonreplicating, live attenuated uracil auxotroph vaccine strains in the type II Deltaku80 genet

246 A purine (but not a pyrimidine) auxotroph was considerably less lethal than wild type; g

247 A double auxotroph was generated that allowed for complete incorp

248 t lacking sulfate transport and a methionine auxotroph was hypersensitive to the CGL inhibitor propar

249 However, the leuD auxotroph was less effective than live BCG in reducing o

250 ng cultures of a homoserine and a methionine auxotroph was measured by mass spectrometry.

251 Growth of tryptophan (Trp-) auxotrophs was more strongly inhibited by phytosphingosi

252 ubiquinol oxidase from Escherichia coli with auxotrophs was used to characterize the hyperfine coupli

253 When a DeltasepS mutant (a cysteine auxotroph) was grown with (34)S-labeled sulfide and unla

254 fect observed in tumor cells, starving yeast auxotrophs wastes glucose as a consequence of the failur

255 ars to be limited in proline because proline auxotrophs were defective for intramacrophage survival a

256 Twenty-four adenine auxotrophs were identified that exhibited supersensitivi

257 When these auxotrophs were inoculated i.p. into C57B6 mice bearing

258 Hybrids able to complement the auxotrophs were purified to homogeneity and assayed for

259 mutants, S. cerevisiae purine and pyrimidine auxotrophs were severely deficient in survival, consiste

260 ngineering Salmonella typhimurium amino acid auxotrophs which grow in viable as well as necrotic area

261 nutritional mutants, including nicotinamide auxotrophs, which are found frequently among cystitis is

262 C. neoformans adenine auxotrophs, which are less virulent in mammals, were als

263 Previously, we showed that a BCG leucine auxotroph with a transposon disruption of the leuD gene

264 Likewise, a spontaneous revertant of an auxotroph with reduced levels of CO dehydrogenase and wi

265 plementation analysis of an E. coli cysteine auxotroph with the pMEK34-14 recombinant plasmid contain

266 Upon transformation of these auxotrophs with CoGeLs, cells growing without supplement

267 In contrast, methionine auxotrophs with defects in the transcription factors Met

268 elta orm2Delta cells: the cells are inositol auxotrophs with impaired transcriptional regulation of g

269 The isolation of thiamine auxotrophs with mutations in at least five different gen

270 d was used to construct a variety of proline auxotrophs with mutations in the proABC locus.

271 ersion of the corresponding Escherichia coli auxotrophs with the same equipment and procedures.

272 a portion of IJs grown on para-aminobenzoate auxotrophs, X. nematophila does not exhibit abortive col

273 It was established that a methionine auxotroph yeast strain could grow on either form of L-me

274 tionally lethal mutants of Escherichia coli (auxotrophs) yielded several de novo sequences, termed Sy