ライフサイエンスコーパス: auxotrophic

1 ryptophan, for which our wild-type strain is auxotrophic.

2 thine transcarbamylase, making them arginine auxotrophic.

3 , whereas the DeltatyrA DeltafolX strain was auxotrophic.

4 Thus, an auxotrophic amber mutation in the pyrE2 gene can be comp

5 osome map was constructed by transduction of auxotrophic and cryptic transposon insertions, with know

6 loss of lgp-tubule formation identified one auxotrophic and nine prototrophic mutants.

7 orally administered live, attenuated, purine auxotrophic B. melitensis WR201 bacteria for their abili

8 here is also a strong stress response in the auxotrophic batch cultures, cell cycle arrest, if it occ

9 a previously uncharacterized mutant glycine auxotrophic cell line, rescued the glycine auxotrophy.

10 reening technology using bacterial phages or auxotrophic cell lines.

11 e to restore growth of Escherichia coli BCAA auxotrophic cells, but SlBCAT1 and -2 were less effectiv

12 activity using functional complementation of auxotrophic cells.

13 Arg-auxotrophic challenge also transcriptionally upregulates

14 s evaluated for its ability to complement an auxotrophic chorismate mutase deletion strain.

15 omologous recombination utilizing the uracil auxotrophic COM1 parent strain (DeltapyrF).

16 ptation to temperature stress were probed by auxotrophic complementation of yeast with prokaryotic, t

17 lishing communities successfully obtained an auxotrophic composition in a nutrition-dependent manner,

18 odel of NP-C disease during anaerobiosis, an auxotrophic condition that requires yeast to utilize exo

19 Furthermore, the auxotrophic cultures express autophagy genes at substant

20 rited the SXI2a-URA5 transgene became uracil-auxotrophic due to silencing of the URA5 gene.

21 Subsequent in vivo selection in an auxotrophic E. coli host yielded functional hybrid enzym

22 lected for by their ability to complement an auxotrophic E. coli strain.

23 essfully tested for functional hybrids in an auxotrophic E.coli host strain.

24 rofolate reductase (mDHFR), in an isoleucine auxotrophic Escherichia coli host strain suspended in 5T

25 Complementation of a cysteine auxotrophic Escherichia coli strain NK3 led to the isola

26 incorporation was achieved using a cysteine auxotrophic Escherichia coli strain.

27 ndosymbiosis, the marriage of an alga and an auxotrophic eukaryote.

28 Auxotrophic for 8 of the 10 essential amino acids, H. de

29 A cbiB mutant strain of Halobacterium was auxotrophic for adenosylcobinamide-GDP, a known de novo

30 archaeon Halobacterium sp. strain NRC-1 was auxotrophic for adenosylcobyric acid, a known intermedia

31 growth experiments, we found that S. suis is auxotrophic for Arg, Gln/Glu, His, Leu, and Trp in chemi

32 In vitro, S. gordonii is conditionally auxotrophic for arginine in monoculture but biosynthesis

33 he disruption of which renders the bacterium auxotrophic for arginine, was expressed and its recombin

34 ranscarbamoylase, several types of tumor are auxotrophic for arginine.

35 ular carcinomas (HCCs) have been shown to be auxotrophic for arginine.

36 ininosuccinate synthetase (ASS1) making them auxotrophic for arginine.

37 species require exogenous B vitamins (i.e., auxotrophic for B vitamins), the possible importance of

38 disruption mutants of ilvI and lrp were both auxotrophic for BCAA in CDM and attenuated compared to w

39 scenario in E. coli, L. lactis appears to be auxotrophic for biotin in that it lacks a full biotin bi

40 mutant of Burkholderia pseudomallei that is auxotrophic for branched chain amino acids.

41 nlike land plants, many eukaryotic algae are auxotrophic for certain B vitamins.

42 rtain mutant Chinese hamster ovary cells are auxotrophic for cholesterol because they fail to carry o

43 carrying a null allele of the cobY gene was auxotrophic for cobinamide-GDP, a known intermediate of

44 Disruption of cysH rendered Mtb auxotrophic for cysteine and methionine, and attenuated

45 trains containing null mutations in lipB are auxotrophic for either lipoic acid or octanoic acid.

46 ylserine (PtdSer) synthase/decarboxylase are auxotrophic for ethanolamine, which must be transported

47 ondrial function, filamentous growth, and is auxotrophic for ethanolamine.

48 ARPE-19 cells became auxotrophic for growth on uridine after eight passages i

49 (P. falciparum nucleoside transporter 1) are auxotrophic for hypoxanthine, inosine, and adenosine und

50 detectable GS protein or GS activity and was auxotrophic for L-glutamine.

51 of the S. typhimurium A1-R mutant, which is auxotrophic for leu-arg and has increased antitumor viru

52 The S. typhimurium A1-R mutant, which is auxotrophic for leu-arg, is tumor-seeking and has antitu

53 to redox cycling agents and dioxygen and are auxotrophic for lysine when grown in air, and expression

54 ressed in an Escherichia coli strain that is auxotrophic for methionine and grown in the presence of

55 cobacterium tuberculosis metA mutant that is auxotrophic for methionine is unlike other auxotrophic m

56 HMG-CoA synthase gene rendered the organism auxotrophic for mevalonate and severely attenuated in a

57 an malaria parasite Plasmodium falciparum is auxotrophic for most amino acids.

58 Bordetella bacterial species are known to be auxotrophic for nicotinamide or nicotinic acid.

59 ed an intracellular ornithine pool, and were auxotrophic for ornithine or polyamines.

60 nt promastigotes and axenic amastigotes were auxotrophic for polyamines and capable of robust growth

61 This Deltaspdsyn strain was auxotrophic for polyamines, required spermidine for grow

62 ender the insect vector form of the parasite auxotrophic for polyamines.

63 We then rendered the yeast auxotrophic for production of the donor nucleotide-sugar

64 rain, whereas the proC and trpD mutants were auxotrophic for proline and tryptophan, respectively.

65 he F. tularensis LVS delta purMCD mutant was auxotrophic for purines when grown in defined medium and

66 Leishmania are auxotrophic for purines, and consequently purine acquisi

67 ementation in Saccharomyces cerevisiae cells auxotrophic for purines, several highly functional noncy

68 ehyde 3-phosphate and E4P dehydrogenases are auxotrophic for pyridoxine.

69 ctron transport complex activities, and were auxotrophic for pyruvate and uridine.

70 s lacking a functional respiratory chain are auxotrophic for pyruvate, which serves as an exogenous e

71 Legionella pneumophila is auxotrophic for several amino acids including cysteine,

72 The symbionts appear to be auxotrophic for some vitamins, but have the potential to

73 an obligate intracellular bacterium that is auxotrophic for the aromatic amino acids and histidine,

74 All models were predicted to be auxotrophic for the vitamins niacin (vitamin B3) and thi

75 hibition, suggesting that T. gondii might be auxotrophic for this cofactor.

76 l growth within human macrophages, which are auxotrophic for Trp and Phe, but is dispensable for grow

77 mutagenized using Tn5, and strains that were auxotrophic for tryptophan (Trp(-)) were isolated.

78 P. horikoshii is auxotrophic for tryptophan and histidine and is unable t

79 d altered stress response, in cells that are auxotrophic for tryptophan.

80 Yeast are auxotrophic for unsaturated fatty acids when grown under

81 fabB mutants were isolated; the mutants were auxotrophic for unsaturated fatty acids.

82 These cells lack mtDNA and are auxotrophic for uridine and pyruvate, which may explain

83 of the strains modeled were predicted to be auxotrophic for vitamins niacin (vitamin B3), thiamin (v

84 ment length polymorphism (RFLP) markers, two auxotrophic genes (arg1, nic1), mating type (matA+/matA-

85 These auxotrophic GROs possess alternative genetic codes that

86 ants of yeast (Saccharomyces cerevisiae) and auxotrophic growth experiments.

87 Streptococcus pyogenes, a multiple-auxotrophic human pathogen, regulates virulence gene exp

88 Such marker-free auxotrophic Leishmania cells show potential as an attenu

89 S. typhimurium A1 is auxotrophic (Leu/Arg-dependent) but apparently receives

90 A1 is auxotrophic (leucine-arginine dependent) but apparently

91 Different auxotrophic marker genes can be used to separate co-clon

92 microarray hybridizations to reliably map an auxotrophic marker, a Mendelian trait, and a major-effec

93 d into the URA3 selectable/counterselectable auxotrophic marker.

94 Any of a variety of auxotrophic markers can recombine with each other, and t

95 The isolation of auxotrophic markers is important for molecular genetic s

96 However, the isolates differ in their auxotrophic markers, in the pI values of their dominant

97 In strains with several auxotrophic markers, loss of DOT4 ubiquitin hydrolase ac

98 The linear chromosome is almost devoid of auxotrophic markers, which probably explains why it was

99 prototrophs from strains with complementing auxotrophic markers; these contained both MTL alleles an

100 esidues, introduced by Met replacement using auxotrophic Met(-) Escherichia coli strains, with glycoa

101 or many years it has been known that thymine auxotrophic microorganisms undergo cell death in respons

102 bicin from a monolayer of two cell lines: an auxotrophic mutant of Chinese hamster ovary cells, AUXB1

103 Here we report that an auxotrophic mutant of M. tuberculosis defective in the d

104 h phenotype and vaccine efficacy of a lysine auxotrophic mutant of Mycobacterium tuberculosis strain

105 A purine-auxotrophic mutant of P. aeruginosa was unable to replic

106 We cloned the PA gene into an auxotrophic mutant of Salmonella enterica serovar Typhim

107 We tested a live, attenuated, purine-auxotrophic mutant strain of Brucella melitensis, WR201,

108 An adenine-requiring auxotrophic mutant strain of P. aeruginosa was isolated

109 We identified an auxotrophic mutant with a transposon insertion in the ge

110 Mice inoculated intraperitoneally with the auxotrophic mutant, 35 days prior to challenge, were pro

111 xS, but not pdxT, resulted in a vitamin B(6) auxotrophic mutant.

112 rated for a community of four E. coli double auxotrophic mutants and is then applied to a gut microbi

113 allele resulted in the generation of leucine auxotrophic mutants by homologous recombination in 6% of

114 h the DeltaglnA1EA2 and DeltaglnA1 glutamine auxotrophic mutants conferred protection on C57BL/6 mice

115 Auxotrophic mutants of Mycobacterium tuberculosis have b

116 When cells of two auxotrophic mutants of Sulfolobus acidocaldarius are mix

117 t by determining the symbiotic competence of auxotrophic mutants of the bioluminescent bacterium Vibr

118 We used auxotrophic mutants of the extremely thermoacidophilic a

119 s auxotrophic for methionine is unlike other auxotrophic mutants of this important species as methion

120 nes in the respective Escherichia coli m-DAP auxotrophic mutants restored the mutants to DAP prototro

121 tion for amino acids or other metabolites in auxotrophic mutants results in rapid loss of viability.

122 Growth media and auxotrophic mutants strongly influenced respiratory meta

123 serine was used to identify Escherichia coli auxotrophic mutants that exhibited limited growth in hum

124 Phagocytosis of the uracil auxotrophic mutants triggered a respiratory burst in neu

125 However, when attenuated, hyperinvasive auxotrophic mutants were used, the tumor-targeting and a

126 hrough the isolation and characterization of auxotrophic mutants, has emerged as a paradigm for the r

127 Tn-4351 insertions resulted in auxotrophic mutations and motility mutations.

128 date strain with two independent attenuating auxotrophic mutations in leucine and pantothenate biosyn

129 metabolic labeling with amino acids rely on auxotrophic mutations of one or more genes on the amino

130 These findings indicate that neither auxotrophic mutations, nor vaccinia virus deficient for

131 ia with mevalonate permitted isolation of an auxotrophic mvaA null mutant that was attenuated for vir

132 CAT enzymes to store cholesterol in a sterol-auxotrophic organism such as Toxoplasma, and furthermore

133 to defined media/feed as well as amino acid auxotrophic organisms.

134 targets for inhibitor design in these purine-auxotrophic organisms.

135 Second, a putrescine auxotrophic, ornithine decarboxylase-deficient Chinese h

136 s proposed to inhibit the in vitro growth of auxotrophic pathogens.

137 Lyso-PtdCho supports the growth of a choline auxotrophic pem1Delta pem2Delta strain.

138 invasiveness was independent of the guanine auxotrophic phenotype and fully recovered when the chrom

139 bsence of polyamine supplementation, but the auxotrophic phenotype could be bypassed by addition of e

140 ivity in vitro and complement its methionine auxotrophic phenotype in vivo.

141 ty to complement the sn-glycerol-3-phosphate auxotrophic phenotype of a plsB mutant strain of E. coli

142 sense mutations are unable to complement the auxotrophic phenotype of met11(-) yeast cells and show l

143 as conducted for suppressors of the inositol auxotrophic phenotype of the ino4-8 mutant of yeast.

144 the SCON2 F-box, we have generated a sulfur auxotrophic phenotype previously unobserved in any scon-

145 found that deletion of bioW caused a biotin auxotrophic phenotype whereas deletion of bioI did not.

146 de 1) an assay for APLT function based on an auxotrophic phenotype, 2) direct demonstration of APLT a

147 treptococcus pneumoniae did not result in an auxotrophic phenotype.

148 observed striking differences in the lysine auxotrophic phenotypes of these three species of mycobac

149 ular metabolism of marine vitamin-requiring (auxotrophic) phytoplankton and bacteria would likely be

150 mutants of S. pneumoniae which have lost the auxotrophic requirement for choline and which were also

151 The auxotrophic requirement for cobinamide-GDP was corrected

152 6Chi and R6Cho(-)--that have lost the unique auxotrophic requirement of this bacterial species for ch

153 rows well in mineral salts medium and has no auxotrophic requirement.

154 a sole source of an amino acid to satisfy an auxotrophic requirement.

155 educed colony size, 4% had died out, 55% had auxotrophic requirements (increasing to 80% after 60 cyc

156 an utilize enkephalins to satisfy amino acid auxotrophic requirements for growth.

157 Starvation of yeast cultures limited by auxotrophic requirements results in glucose wasting and

158 wth of GBS, we assessed the stk1-mutants for auxotrophic requirements.

159 were regulated by the availability of their auxotrophic requirements.

160 report that an immediate-early event of Arg-auxotrophic response involves reactive oxygen species-me

161 ed multiple inter-regulatory pathways in Arg-auxotrophic response, consisting of Axl, c-Myc and ASS1,

162 The mice immunized with the auxotrophic Salmonella vaccine strain with the deletion

163 ementation of the culture medium of the heme-auxotrophic SCV with heme, but not iron, restored growth

164 e with the mutated gene to create an adenine-auxotrophic strain (LSU-E2).

165 Because a sulfur auxotrophic strain is the only requirement of the approa

166 A methionine auxotrophic strain lacking CYS3 was able to grow in medi

167 to alkaliphilic Bacillus firmus OF4811M (an auxotrophic strain of B. firmus OF4) facilitated the ext

168 In this study, an adenine-auxotrophic strain of Edwardsiella ictaluri was construc

169 ntitative levels into proteins made in a Met-auxotrophic strain of Escherichia coli cultured in Met-d

170 We report here a modified auxotrophic strain of Salmonella typhimurium that can ta

171 li and by complementation of wdura5Delta, an auxotrophic strain of Wangiella dermatitidis (Wd) with a

172 est this concept, a budding yeast spermidine auxotrophic strain was found to grow almost normally wit

173 e free PABA and thus allows the growth of an auxotrophic strain.

174 organism, we have constructed ura3 and his3 auxotrophic strains by deleting the relevant coding regi

175 yl-tRNA synthetase (TrpRS) purified from six auxotrophic strains for changes in amino acid activation

176 odeficient individuals, we have studied five auxotrophic strains of BCG produced by insertional mutag

177 These results suggest that auxotrophic strains of BCG represent a potentially safe

178 to hemoglobin (Hb) by expressing the gene in auxotrophic strains of Escherichia coli.

179 d a collection of spontaneous mutants of DAP-auxotrophic strains of Mycobacterium smegmatis that can

180 s been shown to cause toxicity in tryptophan auxotrophic strains of yeast because this bioactive cera

181 bilities that indicate both prototrophic and auxotrophic strains.

182 ests that this gene product is absent in the auxotrophic strains.

183 -2H1,13C1]-tryptophan was fed to the Trp-His auxotrophic Streptomyces coelicolor strain WH101.

184 in these tumors and how they respond to Arg-auxotrophic stress are poorly understood.

185 ormed by residues conserved among tryptophan-auxotrophic TDOs.

186 Because Plasmodium parasites are purine auxotrophic, they must import purines from their host to

187 Analysis of auxotrophic thiH mutants defined two classes, those that

188 two Salmonella vaccine strains attenuated by auxotrophic traits or by the regulated delayed attenuati

189 as been in clinical trials for targeting Arg auxotrophic tumors by Arg starvation therapy.

190 nine, may be a useful treatment for arginine auxotrophic tumors including melanoma and HCC.

191 s for improving the efficacy of treating Arg-auxotrophic tumors using Arg-deprivation strategies.

192 ding the production of recombinant vaccines, auxotrophic vaccines, DNA vaccines and subunit vaccines.

193 These results suggest that amino acid auxotrophic virulent bacteria, which selectively infect

194 We expressed PbENT1 in purine auxotrophic yeast and used radiolabeled substrate uptake

195 Complementation in auxotrophic yeast demonstrated reduced pyrimidine synthe

196 Inositol starvation of auxotrophic yeast interrupts glycolipid biosynthesis and

197 und that the deletion of OLE1 resulted in an auxotrophic yeast strain (designated OLE1 KO) that requi

198 Lysine auxotrophic yeast was grown in the presence of 13C-label

199 ain and compared them with those of a lysine auxotrophic yeast.