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1 cell wall synthesis and diaminopimelic acid auxotrophy.
2 synthesize asparagine and confers asparagine auxotrophy.
3 PDX2 in C. nicotianae results in pyridoxine auxotrophy.
4 ce the succinyl-CoA requirement relieved the auxotrophy.
5 loss of the URA3 marker and return to uracil auxotrophy.
6 desaturases are unable to complement the UFA auxotrophy.
7 nositol phospholipid metabolism and inositol auxotrophy.
8 protein receptors and second for cholesterol auxotrophy.
9 e pyrimidine biosynthetic pathway and uracil auxotrophy.
10 equently, deletion of ilvA causes isoleucine auxotrophy.
11 romycin B-resistant transformants for uracil auxotrophy.
12 deficient DHODH gene alleles induced uracil auxotrophy.
13 produces temperature-sensitive myristic acid auxotrophy.
14 e; (ii) thermosensitive lethality; and (iii) auxotrophy.
15 ol methyl oxidase ERG25 gene leads to sterol auxotrophy.
16 gesterone toxicity resulted from cholesterol auxotrophy.
17 o exhibit yet another phenotype, an inositol auxotrophy.
18 phosphate pathway can rescue sod1 methionine auxotrophy.
19 novo pyrimidine biosynthesis induced uracil auxotrophy.
20 donii and the basis for conditional arginine auxotrophy.
21 ankia microsymbionts to atypical patterns of auxotrophy.
22 f one or a few key genes have led to vitamin auxotrophy.
23 rulence in mice despite retaining pyrimidine auxotrophy.
24 rotein production costs and compensating for auxotrophy.
25 etic cost, and did not consider lifestyle or auxotrophy.
26 e auxotrophic cell line, rescued the glycine auxotrophy.
27 of FPP leading to growth defects and sterol auxotrophy.
28 t genetic linkage to swarming mutations, and auxotrophy.
29 nolamine dehydratase, PhhB, rescues tyrosine auxotrophy.
30 duced INO1 expression and conferred inositol auxotrophy.
31 cell surface polysaccharides and a cysteine auxotrophy.
32 te to 3-phosphoglycerate, exhibited inositol auxotrophy.
33 re more sensitive to heat-induced methionine auxotrophy.
34 the SepRS-encoding gene resulted in cysteine auxotrophy.
35 ith numerous putative amino acid and vitamin auxotrophies.
36 of the seo mutants to suppress the sod1Delta auxotrophies.
37 he known air-dependent methionine and lysine auxotrophies.
38 y, GEMs were used to predict strain-specific auxotrophies.
39 trains carrying one (CAI-4) to three (BWP17) auxotrophies.
40 e enzymes in sod1 Delta yeast results in the auxotrophies.
41 we present evidence that the human Inositol auxotrophy 80 (Ino80) SNF2 ATPase is subject to regulati
42 ylogenetically unrelated distribution of B12 auxotrophy across the algal lineages suggests that the M
45 The Deltacps lesion conferred pyrimidine auxotrophy and a growth requirement for medium supplemen
46 he 2-hybrid system, as assessed by histidine auxotrophy and beta-galactosidase activity, was disrupte
48 droxyl of the inositol ring, causes inositol auxotrophy and decreased intracellular inositol and phos
49 The gua mutations caused guanine/guanosine auxotrophy and led to partial derepression of direct Cod
52 mitochondrial DNA was determined by uridine auxotrophy and quantitative real-time polymerase chain r
53 e lacking exon 1 overcame the cell's glycine auxotrophy and restored intracellular glycine concentrat
54 C; all of these characteristics, as well as auxotrophy and slow-growth rate, were reversed by transc
55 e that we have defined the cause of the glyB auxotrophy and that the glyB mft mutation identified a r
56 in clinical medicine whereby both amino acid auxotrophy and the immunoregulatory pathways controlled
57 n complementation of the strain's fatty acid auxotrophy and the production of Delta11Z-unsaturated fa
58 imilation control protein (NAC) relieved the auxotrophy, and addition of compounds that could increas
59 y, one mutant (D335A) did not complement the auxotrophy, and another (R377A) allowed only minimal gro
60 agenized the cells, selected for cholesterol auxotrophy, and identified two mutant cell lines (SRD-12
61 d by amplification phylotyping, nicotinamide auxotrophy, and outer membrane protein patterns (OMPs).
63 I; confer phosphate, galactose, and inositol auxotrophies; and fail to activate PHO5, GAL10, and INO1
65 ial-diatom model system based on vitamin B12 auxotrophy as a sensitive assay for metabolite exchange
66 hesis, in the pyc::tn strain reverted biotin auxotrophy, as did reconstituting the last step of the p
68 owever, previous attempts to generate uracil auxotrophy by genetically deleting the mitochondrion-ass
69 ll wall peptides, we have found that proline auxotrophy can be satisfied with the peptide Pro-Phe-Lys
71 expression rescued aco1 yeast from glutamate auxotrophy, cells remained growth-limited by glutamate,
72 elatively small genome and multiple putative auxotrophies characteristic of Ca. U. copiosus suggest t
73 oach uses yeast homologous recombination, an auxotrophy complementation-based yeast selection system
74 rowth in medium without polyamines; however, auxotrophy could be rescued by spermidine but not by put
75 5-diaminopentane (cadaverine), but polyamine auxotrophy could not be overcome by other aliphatic diam
77 ne biosynthesis, and in addition to cysteine auxotrophy, cys4 mutants have much lower levels of intra
80 ments that led to complementation of thymine auxotrophy during intracellular growth of the bacterium
81 ts show that the clk-1 mutations result in Q auxotrophy evident only when Q is removed from the diet,
82 ation of [O2-] imposes, on Escherichia coli, auxotrophies for branched chain, sulfur-containing, and
84 toplasmic superoxide dismutase (SOD) exhibit auxotrophies for sulfur-containing, branched-chain, and
87 creened for a range of phenotypes, including auxotrophy for amino acids, inability to reduce Fe(III)
92 ic conditions the deletion of IscS caused an auxotrophy for thiamine and nicotinic acid, whereas unde
94 ockout strains were evaluated for pyrimidine auxotrophy, for attenuation of virulence, and for their
96 dent methionine synthase, and that cobalamin auxotrophy has arisen numerous times throughout evolutio
97 llectively, our data demonstrate that uracil auxotrophy has cell type-specific effects on the fate of
99 affects all markers tested: three different auxotrophies (histidine, purine, and cobalamin) and resi
101 alanine completely eliminates the methionine auxotrophy imposed by diamide treatment, suggesting that
102 mal gene is capable of complementing leucine auxotrophy in a leuB(-) strain lacking the paralogous is
104 ng constitutive promoter leads to methionine auxotrophy in B. subtilis, suggesting that S-adenosylmet
106 studies of SCV S. maltophilia have suggested auxotrophy in hemin, methionine, and thymidine associate
107 a novel model for the evolution of metabolic auxotrophy in microorganisms that arises as a result of
109 , and are shown here to result in a thiamine auxotrophy in some of the strains tested, including S. e
111 itivity to oxidative stress, (ii) a thiamine auxotrophy in the absence of the YggX protein, and (iii)
115 ds, substantially, current knowledge of haem auxotrophy in ticks and underscores the importance of ha
116 oxide dismutase (Sod1p) is an aerobic lysine auxotrophy; in the current work we show an additional le
117 met6) exhibits methionine as well as adenine auxotrophy indicating that MS is required for methionine
118 A fur mutation only partially relieved the auxotrophies, indicating that Fur derepression assists b
119 tion of P. aeruginosa cysH produced cysteine auxotrophy, indicating its role in sulfate assimilation.
120 H, and proI) were required to confer proline auxotrophy, indicating that the products of these genes
121 conclude that when Sod1p is absent a lysine auxotrophy is induced because Lys4p is inactivated in th
122 genetic make-up of the cell, as reflected in auxotrophy, is hence likely to be a determinant of gene
123 Given the indispensable role of heme, this auxotrophy may be exploited to develop drugs that interf
124 tant, mc21278 (ask1::aph), exhibits multiple auxotrophy (Met-, Thr-, DAP-, and Lys-) and is complemen
125 f haploid selection is mating-type-regulated auxotrophy (MRA), by which prototrophy is restricted to
126 or fermentable carbon and the branched-chain auxotrophy occur because superoxide (O2-) leaches iron f
127 t in opposition: (i) complete suppression of auxotrophy occurs by overexpression of GreA or DksA only
129 antial evidence that the sulfur and aromatic auxotrophies of SOD mutants are also directly or indirec
130 es of ATX2 were found to reverse the aerobic auxotrophies of sod1(delta) mutants for lysine and methi
131 Overexpression of the alsK gene relieves the auxotrophy of a glucokinase-deficient bacterium, demonst
132 -Huet anomaly fail to rescue the cholesterol auxotrophy of a LBR-deficient human cell line, consisten
133 DksA overexpression partially suppresses the auxotrophy of a ppGpp-deficient strain; (iii) microarray
134 icum panD(+) gene corrected the pantothenate auxotrophy of a S. enterica yhhK strain, supporting in v
135 and was capable of suppressing the inositol auxotrophy of a second ino4 missense mutant, ino4-26, as
136 active (i.e. failed to rescue the methionine auxotrophy of a shm2Delta ade3 strain) complemented the
137 e ability of PfPMT to complement the choline auxotrophy of a yeast mutant defective in phospholipid m
139 thaliana is able to complement the cysteine auxotrophy of an Escherichia coli cysH [3'-phosphoadenos
141 aromyces cerevisiae mutant to model the heme auxotrophy of C. elegans and demonstrate that, under hem
142 The subcloned cDNA complemented the glycine auxotrophy of glyB cells and reinstated folate accumulat
143 ne suppresses the choline-sensitive inositol auxotrophy of mpk1Delta cells, whereas overexpression of
144 ver, M+ mutations, which suppress amino acid auxotrophy of ppGpp degrees strains and which have been
145 f PpMS and its ability to reverse methionine auxotrophy of Ppmet6 Thus, association of two PpMS monom
146 is unable to reverse methionine and adenine auxotrophy of Ppmet6 Thus, nuclear localization is essen
147 pathway activity contributes to the inositol auxotrophy of sac1 strains in a novel manner that does n
148 hat rescues the sphingolipid long-chain base auxotrophy of Saccharomyces cerevisiae SPT mutants when
149 ns explain the characteristic nicotinic acid auxotrophy of Shigella organisms and are consistent with
150 r, these findings indicate that the inositol auxotrophy of snf1Delta strains arises in part from elev
154 temperature-sensitive unsaturated fatty acid auxotrophy of strain M6 [fabA6 (Ts)] were isolated from
157 e or orotate could circumvent the pyrimidine auxotrophy of the Deltacps/Deltauprt double knockout.
158 (ino1Delta/hINO1) complemented the inositol auxotrophy of the mutant and led to inositol excretion.
163 ate lyase mRNA, suggesting that the arginine auxotrophy of these cells was a result of an inability t
166 udy, we determined the effect of nutritional auxotrophy on the ability of L. pneumophila to survive i
167 in sensitivity to ampicillin, in nutritional auxotrophies, or temperature-sensitive growth were mappe
168 intracellular replication was due to leucine auxotrophy per se and not to a polar effect of the trans
169 nzymatically inactive gdhA1 allele showed no auxotrophy, repression of GDH expression by the nitrogen
171 a coli WP2 bacteria with an ochre amino acid auxotrophy show no evidence of growth during the first f
172 17 double disruption also leads to histidine auxotrophy, similar to the adenine/histidine auxotrophy
173 to be a bradytrophy, rather than an absolute auxotrophy, since lack of Cys merely imposed a growth la
175 d research, strategies to harness amino acid auxotrophy so as to block cancerous lymphocyte growth ha
177 25% of the insertions resulted in amino acid auxotrophy, suggesting that insertion was random at a gr
178 tructed stable haploid strains with multiple auxotrophies that will facilitate molecular and genetic
179 how that loss of Ino1 results in an inositol auxotrophy that can be rescued only partially by exogeno
181 e TPP (null mutants) and those with thiamine auxotrophy that was corrected by either L-tyrosine or th
182 high-copy-number clones of gdhA displayed an auxotrophy that was interpreted as a limitation for alph
183 ion, inducible toxin switches and engineered auxotrophies, these approaches are compromised by cross-
185 Using Northern analysis, we have traced this auxotrophy to a complete shutdown of cys-3+ gene express
187 ic assay based on phenotypic conversion from auxotrophy to prototrophy was established to select effi
188 c capacity and exploitation of the resulting auxotrophy to signal a particular host environment.
189 of visceral leishmaniasis, confer polyamine auxotrophy to the insect vector or promastigote form of
190 that suppression of the sod1Delta methionine auxotrophy was dependent on the pentose phosphate pathwa
193 hiamin (vitamin B1), whereas strain-specific auxotrophies were predicted for riboflavin (vitamin B2),
195 ve growth phenotype of pos5 and its arginine auxotrophy were repaired by plasmid-borne POS5 but not U
196 it of the dehydrogenase) exhibit a glutamate auxotrophy when ammonium is the sole source of nitrogen.
198 hat it can repair the unsaturated fatty acid auxotrophy when it is expressed in a Saccharomyces scspt
199 ese two chromosomal genes results in adenine auxotrophy, whereas expression of either gene alone is s
200 Deletion of OMPDC induced a severe uracil auxotrophy with loss of replication, loss of virulence i
201 hat synchronization of bacterial amino acids auxotrophy with the host is a driving force in pathogeni
203 pathway exhibit a choline-sensitive inositol auxotrophy, yet fully derepress INO1 and other Opi1p-reg
204 linositol 4,5-bisphosphate, exhibit inositol auxotrophy, yet fully derepress INO1, encoding inositol-
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