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1 avidin compared to binding of HABA to native avidin.
2 iotin and its site-specific accessibility to avidin.
3 n streptavidin and is one residue removed in avidin.
4 er cooperative hydrogen-bonding effects than avidin.
5 on the well-known interactions of biotin and avidin.
6 cognition of a biotinylated protein layer by avidin.
7 ed to bind within the biotin-binding site of avidin.
8 rane by biotinylation and cross-linking with avidin.
9 rines forming a major physiological role for avidin.
10 the specific interaction between biotin and avidin.
11 steine, lysine, or arginine functions of the avidin.
12 f a biotin-derivatized caged-xenon sensor to avidin.
13 tion opposite to the protein analytes, as in avidin.
14 ondrial extracts by using peroxidase-coupled avidin.
15 nose and hybrid-type N-glycans purified from avidin.
16 blized, and exposed to fluorescently labeled avidin.
17 tivity between the anion and its position in avidin.
18 s then used to selectively detect the target avidin.
19 tin conjugate followed by staining with FITC-avidin.
20 inding interaction analyses, an avidin/HABA (avidin/4'-hydroxyazobenzene-2-carboxylic acid) competiti
22 ing a pH-sensitive fluorophore conjugated to avidin-Ab tetramers, we found that CD244 crosslinking in
23 configuration is consistent with the biotin-avidin accessibility and internal salt-bridge data used
25 f residues throughout the KvAP channel using avidin accessibility to different-length tethered biotin
26 th (imino)biotinylated cargo proteins via an avidin adaptor with a high degree of spatial control.
29 agent, which can be used in conjunction with avidin affinity chromatography to purify biotinylated pe
30 pirolactone biotinylation, and enrichment by avidin affinity chromatography with mass spectrometry.
31 e was purified from M. tuberculosis H37Rv by avidin affinity chromatography, and the three major prot
34 for the isolation of the labeled protein by avidin affinity precipitation, facilitating efforts to i
35 olumn, and after cyanogen bromide treatment, avidin affinity purification was used to capture Bio-DOP
37 dihydrofolate reductase (DHFR) locus, using avidin-affinity purification of biotinylated chromatin f
39 The detection was performed after adding avidin-ALP to perform avidin-biotin reaction; the signal
40 tion method where NP probe concentration and avidin analyte were unknown and both were determined.
43 n-ligand and protein-protein systems: biotin-avidin and biotin-streptavidin, barstar-dibarnase and Z
44 w that SBMs reduce nonspecific adsorption of avidin and BSA on PDMS by 2-3 orders of magnitude, as co
45 pecificity on bovine serum albumin (BSA) and avidin and detecting SEB in artificially contaminated mi
46 he dissociation constants (Kd) of the biotin-avidin and dopamine-boronic acid complexes were determin
48 ng, rather than nonspecific contact, between avidin and functionalized xenon leads to the effects on
50 We find that the interactive forces between avidin and graphene are mainly hydrophobic, along with s
51 erimental approach, the interactions between avidin and graphene for the development of a sensing pla
52 ence spectroscopy by utilizing conjugates of avidin and horseradish peroxidase in a microtiter plate
54 of DMSO on the structure and interactions of avidin and Mycobacterium tuberculosis (Mtb) CYP142A1 wer
56 ity in the hydrogen-bonding network for both avidin and streptavidin by greatly polarizing the urea o
57 ually strong reversible binding of biotin by avidin and streptavidin has been investigated by density
60 ved beta-barrel structure similar to that of avidin and the cation-dependent mannose 6-phosphate rece
61 target due to the interaction between biotin-avidin and the enzyme converts electro-inactive alpha na
63 fully employed to detect surface-immobilized avidin, and a detection limit of 10 ng/mL was achieved.
64 like cations of serum albumin, streptavidin, avidin, and alcohol dehydrogenase were probed using cati
65 proteins, including human thrombin, PDGF-BB, Avidin, and His-tagged recombinant protein, were studied
66 antibody, NeutrAvidin and biotinylated anti-avidin, and the signal for protein binding on the membra
68 duced BAD was biotinylated and accessible to avidin (approximately 60 kDa), myotubes were fixed, perm
71 lected from phage libraries and streptavidin/avidin as core protein were used for direct detection of
72 In contract, the intracellular delivery of avidin, as a non-covalent complex with a biotinylated Ta
74 pon binding of a biotin-containing sensor to avidin at 1.5 muM concentration, the free xenon T2 is re
82 on principles and biomedical applications of avidin-based nanoparticles in drug delivery and diagnosi
83 tic binding energy is predicted to favor the avidin-bicyclic urea complex due to the relatively large
85 exposed to thiolated biotin to introduce an avidin binding element on the surface of the gold beads.
86 mmunoglobulin G (Bt-IgG), which binds to the avidin binding sites distal to the surface and the F(c)
88 PE membranes were determined to be 33 nM for avidin binding to biotinylated lipids, 73.5 nM for chole
92 BSO, and BNB were quantitated by HPLC and an avidin-binding assay in CSF samples from a subset of 11
96 of biotin were determined initially as total avidin-binding substances (TABS) in CSF obtained by lumb
98 This methodology has been tested with the avidin-biocytin binding system for which the best-fit di
99 evaluated using the TS 106 antibody and the avidin biotin labeling immunohistochemical technique in
100 cific non-covalent interactions (e.g. strept(avidin)/biotin) or covalent bond formations (e.g. invers
101 The structures and biochemical properties of avidin, biotin and their respective analogues will also
103 oxylamine to enrich the modified peptides by avidin-biotin affinity chromatography and analyze them b
105 nates the contamination that can result from avidin-biotin based retrieval systems and simplifies dat
106 ignal was monitored in real-time based on an avidin-biotin binding between avidin coated QCM surface
107 ostmortem brain in molecular assays, we used avidin-biotin binding to couple superparamagnetic iron o
108 phospholipid were immobilized through biotin-avidin-biotin binding to the surface of a biotin-modifie
114 ific adsorption, specific adsorption via the avidin-biotin interaction, and immobilization of antibod
119 lds, with biotinylated aptamers attached via avidin-biotin linkages, and horseradish peroxidase (HRP)
121 performed after adding avidin-ALP to perform avidin-biotin reaction; the signal was generated through
122 properties of the coupled moieties makes the avidin-biotin system a versatile platform for nanotechno
126 uple whole-cell recordings with an optimized avidin-biotin-peroxidase staining technique, we carried
129 in ammonium carbonate buffer (which ensures avidin/biotin complexation) reveal that nonspecific inte
130 ed immobilization strategy (i.e., through an avidin/biotin linkage) is well-suited to immobilize a ne
131 IPAM) were conjugated to antibodies using an avidin/biotin system and deposited onto surfaces using a
132 ation as an alternative using either (strept)avidin/biotin, bispecific antibodies, or oligomers.
133 ASPA antibodies throughout the brain by the avidin/biotin-peroxidase detection method, and colocaliz
135 es and the protein varies and, unlike in the avidin:biotin complex, complete ordering of the protein
137 y for detection, and this is amplified by an avidin/biotinylated horseradish peroxidase complex.
138 ified human ATM protein, ATM-DNA and ATM-DNA-avidin bound complexes by single-particle electron micro
139 lobin-alpha, ovalbumin, human transthyretin, avidin, bovine serum albumin, concanavalin, human serum
143 d polymers (CPs) for sensing the presence of avidin by use of a biotin-modified fluorescence quencher
145 nd are specifically targeted to GPI-anchored avidin-CD14 chimeric proteins expressed on the membranes
146 nin I on silanized glass slides using biotin-avidin chemistry, as well as through direct covalent att
147 me based on an avidin-biotin binding between avidin coated QCM surface and specific biotinylated LAMP
149 Proteins were affinity isolated onto (strept)avidin-coated beads and then photoreleased (PC-SNAG).
150 MB probes, we have developed a DNA array on avidin-coated cover slips and have improved analytical s
154 streptavidin-coated magnetic beads (MB) and avidin-coated polystyrene microspheres/beads (PSB) entra
156 tin moieties and spacers were immobilized on avidin-coated surfaces and characterized by surface plas
157 By electron microscopy of the ATP synthase-avidin complex in negative stain and by subsequent image
158 mation and stability of the resulting biotin-avidin complex, it is useful to know the kinetics of the
159 t consistent with a simple mechanism whereby avidin complexation of BPP+ leads to encapsulation of th
160 model proteins, namely, transthyretin (TTR), avidin, concanavalin A (conA), and human serum amyloid P
161 y using either a fluorescent or a peroxidase avidin conjugate, or placement of a fluorescent lipophil
165 wich immunoassay, biotinylated antibody, and avidin-conjugated GOx for the selective naked-eye detect
168 n to the two probes and MALDI-MS analysis of avidin contained in the solution removed from the probe
171 c-coglycolic acid-based NP platform, wherein avidin-decorated NPs can be targeted to multiple human D
172 apture mechanism is specific, such as biotin-avidin, deposition is optimal at medium flow rates with
174 attached to one of two different neutralite avidin-derivatized mAbs directed against either human CD
176 pment of MHC tetramers, based on fluorescent avidins displaying biotinylated peptide-MHC complexes.
178 e by affinity interaction between biotin and avidin; electrochemical impedance measurements were perf
179 functional microstructures from crosslinked avidin enables submicrometer localization of controllabl
181 llected on six model glycoproteins (RNase B, avidin, fetuin, asialofetuin, transferrin, and AGP) as w
182 r dysgenic (alpha1S-null) myotubes, punctate avidin fluorescence co-localized with the XFP puncta for
183 biotinylated bovine serum albumin (BSA) and avidin, followed by binding of a biotinylated oligonucle
185 is described, and the selective isolation of avidin from a three-component mixture of avidin, lysozym
189 that an anti-human transferrin receptor IgG3-avidin fusion protein (anti-hTfR IgG3-Av) inhibits the p
191 aptamer of thrombin was immobilized onto an avidin-graphite epoxy composite (AvGEC) electrode surfac
192 tion-state, binding interaction analyses, an avidin/HABA (avidin/4'-hydroxyazobenzene-2-carboxylic ac
195 Hydrogen peroxide (H2O2) is then reduced by avidin-HRP in the presence of TMB (3,3',5,5'-tetramethyl
197 cal impedance spectroscopy (EIS) analysis of avidin immobilized on a graphenated polypyrrole (G-PPy)
201 dy, we have determined crystal structures of avidin in complex with 8-oxodeoxyguanosine and 8-oxodeox
203 fluidic network that generates a gradient of avidin in solution and immobilizes this protein on the s
205 lar, the ligand-receptor binding (biotin and avidin in this paper) was not interfered with when ancho
206 antibody and binding of fluorescein-labeled avidin indicated that guanine bases were oxidatively mod
208 ta(1-42)] oligomers incorporating the biotin-avidin interaction that has been a workhorse for screeni
211 The method is rapid, making use of biotin-avidin interactions and paramagnetic particles to purify
212 e bicyclic urea are much weaker than (strept)avidin interactions due to relatively low polarization o
213 ol (PIP) with its binding protein and biotin/avidin interactions were employed for array measurements
214 ause the binding affinity between biotin and avidin is large (Ka=10(15) M(-1)), biotin could also ser
216 t assay (ELISA)-based assays of the modified avidin, it was found that there are approximately three
218 nce energy transfer (FRET) experiments using avidin labeled with an Alexa488 fluorophore, which bound
224 (BBIR) composed of an extracellular-modified avidin linked to an intracellular T-cell signaling domai
228 aggregates were formed when Ru(II) subsetPSB/avidin<-->anti-CRP was mixed with anti-CRP<-->MB conjuga
230 t cross-linking of enzymes, by decoration of avidin matrixes with biotinylated enzymes, or by cross-l
231 the surface of cerebellar granule neurons by avidin-mediated cross-linking, and inwardly rectifying p
232 ion curve was achieved between the amount of avidin-metal complex on the cell surface and the emissio
233 ed with the metal-free avidin conjugate, the avidin-metal complex was observed to display a stronger
235 increased with an increase of the number of avidin-metal complexes on the cell surface but the lifet
237 dies illustrate the molecular basis by which avidin might act as a marker of DNA damage, although the
239 Notably, the structure and function of the avidin molecule are largely preserved after its adsorpti
241 distinct regions of a FAEP gel by protein A, avidin, monoclonal anti-BSA antibody, and concanavalin A
242 ano-Assembly (ANANAS) is a kind of soft poly avidin nanoparticle originating from the high affinity i
243 he superior anticoagulant efficacy and rapid avidin neutralizability of EP217609 compared with antico
246 sitive detection of four different proteins, avidin, O6-methylguanine DNA methyltransferase (MGMT), S
247 e nanocomposite confirming the adsorption of avidin on graphene nanoplatelets as observed from the Fo
249 ness of the membrane that forms a barrier to avidin penetration, allowing us to determine the magnitu
252 ried sequence and length were attached to an avidin platform and screened for affinity against the po
253 proteins were enriched by biotinylation and avidin precipitation and analyzed by tandem mass spectro
257 better selectivity than the one prepared via avidin, recognizing almost none of the tested Gram-posit
259 Instead, we propose that binding of BPP+ to avidin results in the quenching unit attaching to a posi
261 strategy is proposed whereby self-quenching avidin-rhodamine X, which has affinity for lectin on can
262 with a background summary of biotin/(strept)avidin self-assembly and the current design rules for cr
267 results show that the binding affinities of avidin, streptavidin, and neutrAvidin for biotin were al
269 A comparison of the binding properties of avidin, streptavidin, neutrAvidin, and antibiotin antibo
270 growth and show the ability to functionalize avidin structures with biotinylated reagents, an approac
273 rations higher than 4% (v/v) destabilize the avidin tetramer toward dissociation and unfolding, via b
274 cess, the geometry of peptide-MHC display by avidin tetramers was examined, as well as the stability
275 hrough strong inert linkages, such as biotin-avidin, that depolymerizing MTs exert a brief tug on the
276 les (pyranine and crystal violet) as well as avidin through melittin induced membrane pores and defec
277 It becomes biotinylated in vivo, and allows avidin to be bound quantitatively to the purified enzyme
279 ed to rapidly determine whether the ratio of avidin to biotinylated NP was optimal or whether additio
280 del is proposed for the selective binding of avidin to DNA containing oxidatively damaged deoxyguanos
282 ication of various surface concentrations of avidin to the two probes and MALDI-MS analysis of avidin
284 nding positively charged microdomains of the avidin, two polyanions, poly(acrylic acid-co-maleic acid
286 periment in which the biotin-binding site of avidin was blocked with native biotin showed no such spe
287 formation of bioconjugates of nanogels with avidin was confirmed using optical fluorescence microsco
288 teractions of biotin and fluorophore-labeled avidin was constructed on SIFs and was subsequently comp
291 arge freedom of movement of the anion inside avidin, we also investigated the substrate scope of this
292 experiments confirmed that poly(guanine) and avidin were immobilized on the surface of silica NPs.
293 (mucin, soybean peroxidase, collagen IV, and avidin) were compared with those of the constituent suga
294 In contrast, this site was accessible to avidin when the identical construct was expressed in dys
295 is effected by sequential immobilization of avidin, which binds to the biotin in the initial templat
296 gn of a long sought after monovalent form of avidin, which would be ideal for novel types of biotechn
299 to the polyacrylamide gel solution to embed avidin within the gel matrix by interaction with the ami
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