ライフサイエンスコーパス: bacmid

1 cular disulfide could not rescue a GP64-null bacmid.

2 nt in cells transfected with the lef-11-null BACmid.

3 into the polyhedrin locus of the vAc(lef6KO) BACmid.

4 elayed in cells infected with the lef-6-null BACmid.

5 e levels seen with the repaired or wild-type bacmids.

6 lts seen with wild-type and vAcAN-KO/GUS-Res bacmids.

7 nation is approached, in which a recombinant bacmid, a shuttle vector that can be propagated in both

8 t in cells transfected with a vlf-1 knockout bacmid, aberrant tubular structures containing the capsi

9 The lef-11-null BACmid and control BACmids were transfected into Sf9 cel

10 ins, we deleted the gp64 gene from an AcMNPV bacmid and inserted F protein genes from three different

11 seen with the vAcAN-KO/GUS-Res and wild-type bacmids at early stages posttransfection.

12 This BACmid, BAC36DeltaK8, displayed an enhanced growth pheno

13 reverse genetics system, the "eight-in-one" bacmids (bcmd-RGFlu) showed higher efficiency of virus r

14 Bacmid bMON14272 contains nine highly homologous regions

15 g counter-selection recombineering to modify bacmid bMON14272, a recombinant baculoviral genome, as w

16 ates 10 of the 12 viral miRNAs from the KSHV bacmid by using recombineering methods.

17 In contrast, rescuing the vlf-1 knockout bacmid construct with a copy of VLF-1 that carries a mut

18 Moreover, methylation of bacmid constructs containing the EBV genome enhances BZL

19 mphenicol acetyltransferase gene (cat) and a bacmid containing the AcMNPV genome in Escherichia coli.

20 n human cytomegalovirus (HCMV, strain AD169) bacmid-derived viruses to test their effects on virus re

21 to those from wild-type AcMNPV or a control (BACmid-derived) virus.

22 re K-Rta is overexpressed, a K-bZIP knockout bacmid displayed an aberrant subcellular localization pa

23 Additionally, analysis of replicated bacmid DNA by field-inversion gel electrophoresis indica

24 Transfection of the gp64-null bacmid DNA into Sf9 cells does not generate infectious p

25 dividual ESCRT-I or ESCRT-III gene and viral bacmid DNA or viral bacmid DNA that expressed DN forms o

26 ESCRT-III gene and viral bacmid DNA or viral bacmid DNA that expressed DN forms of ESCRT-I and ESCRT-

27 However, ac92-knockout bacmid DNA-transfected cells lacked multiply enveloped o

28 says showed no virus spread in ac92-knockout bacmid DNA-transfected insect cells.

29 ither virus infection or the transfection of bacmid DNA.

30 generate influenza A virus in vivo by using bacmid DNAs.

31 We next used recombinant bacmids expressing WT or DN VPS4 proteins to examine vir

32 CMV bacterial artificial chromosome plasmid (BACmid) expressing the nonshuttling UL84 mutant (NS84 BA

33 kworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system.

34 coexpression experiments, both single-mutant bacmids gave rise to infectious virus but the double mut

35 bination in an AcMNPV genome propagated as a BACmid in Escherichia coli.

36 an AcMNPV genome propagated as an infectious BACmid in Escherichia coli.

37 expression assays, studies of a lef-11-null BACmid indicate that LEF-11 is required for viral DNA re

38 tial nature of LEF-4 by RNA interference and bacmid knockout technology.

39 A recombinant BACmid lacking the PAN RNA locus fails to express K-Rta

40 ansfection of insect cells with lef-4 mutant bacmid, no viral progeny was produced, further defining

41 AN-KO/GUS, vAcAN-KO/GUS-Res, and a wild-type bacmid showed that the vAcAN-KO/GUS bacmid was able to r

42 similar to that detected for a gp64 knockout bacmid that served as a noninfectious control virus.

43 MNPV, and a nonpropagating gp64-null control BACmid (vAc(GUSgp64KO)) were readily detected.

44 The viral DNA genome of the lef-11-null BACmid (vAc(lef11KO)) was not amplified, whereas replica

45 The resulting AcMNPV lef-11-null BACmid (vAc(lef11KO)) was unable to propagate in cell cu

46 in cell culture, although a "repair" AcMNPV BACmid (vAc(lef11KO-REP)), which was generated by transp

47 d amplification of the genomes of the repair BACmid (vAc(lef11KO-REP)), wild-type AcMNPV, and a nonpr

48 nexpectedly, the resulting AcMNPV lef-6-null BACmid (vAc(lef6KO)) was able to propagate in cell cultu

49 The lef-6-null BACmid (vAc(lef6KO)) was further examined to determine w

50 Two "repair" AcMNPV BACmids (vAc(lef6KO-REP-P) and vAc(lef6KO-REP-ie1P)) wer

51 thern analysis of Sf9 cells transfected with bacmid vAcAN-KO/GUS showed that transcription of late an

52 The AcMNPV an knockout bacmid (vAcAN-KO/GUS) was unable to propagate in Sf9 cel

53 opagate in Sf9 cells, although an an-rescued bacmid (vAcAN-KO/GUS-Res) propagated normally.

54 ocalization during infection using an AcMNPV bacmid virus that produces a functional AcMNPV FP25K-gre

55 ild-type bacmid showed that the vAcAN-KO/GUS bacmid was able to replicate to levels similar to those

56 n this report, we show that a vlf-1 knockout bacmid was able to synthesize viral DNA at levels simila

57 The vAc(lef11KO) BACmid was examined to determine if the defect in viral

58 o investigate its function, an ac79-knockout bacmid was generated through homologous recombination in

59 2 during virus replication, an ac92-knockout bacmid was generated through homologous recombination in

60 nto the polyhedrin locus of the vAc(lef11KO) BACmid, was able to replicate in a manner similar to wil

61 The lef-11-null BACmid and control BACmids were transfected into Sf9 cells, and viral DNA r

62 An HCMV BACmid with the second Py mutated failed to produce any