ライフサイエンスコーパス: bacteriophage lambda

1 ld higher than ejection forces measured with bacteriophage lambda.

2 r a complete narrative for the life cycle of bacteriophage lambda.

3 perspective on the lysis/lysogeny switch of bacteriophage lambda.

4 lls to foster lytic growth by red gam mutant bacteriophage lambda.

5 ble for maltose uptake and for attachment of bacteriophage lambda.

6 ase required for homologous recombination by bacteriophage lambda.

7 -mediated homologous recombination system of bacteriophage lambda.

8 on of the N and Q antiterminator proteins of bacteriophage lambda.

9 lease activity of the model exonuclease from bacteriophage lambda.

10 transcription through the tR2 terminator of bacteriophage lambda.

11 , a multifragment chloroplast DNA probe, and bacteriophage lambda.

12 ed for the naturally leaderless cI mRNA from bacteriophage lambda.

13 35 consensus regions of the P(R) promoter of bacteriophage lambda.

14 assembly inhibitory factors including Kil of bacteriophage lambda.

15 ages M13 or phiX174 nor of Uracil-containing bacteriophage lambda.

16 and their homologues in the closely related bacteriophage lambda.

17 sically disordered protein, the N protein of bacteriophage lambda.

18 ation signal, to an RNA binding peptide from bacteriophage lambda.

19 inor capsid protein UL25 in HSV-1 and gpD in bacteriophage lambda.

20 hanistic studies on the terminase motor from bacteriophage lambda.

21 UUAU-3' from the tR2 intrinsic terminator of bacteriophage lambda.

22 ern the dynamics of in vitro DNA ejection in bacteriophage lambda.

23 single-molecule studies of DNA packaging in bacteriophage lambda.

24 the lac UV5 promoter or the PR promoter from bacteriophage lambda.

25 of multiple modifications on the surface of bacteriophage lambda.

26 urements of single DNA molecule packaging in bacteriophage lambda.

27 vailing notions in this postgenomic era, the bacteriophage lambda, a paragon of simplicity, may still

28 We propagated bacteriophage lambda, a virus with rapid generations and

29 The N protein of bacteriophage lambda activates expression of the delayed

30 Protein N of bacteriophage lambda activates the lytic phase of phage

31 The tetrameric cII protein from bacteriophage lambda activates transcription from the ph

32 The N protein of bacteriophage lambda activates transcription of genes th

33 The beta protein of bacteriophage lambda acts in homologous genetic recombin

34 We consider a single network derived from bacteriophage lambda and construct a two-parameter deter

35 The development of bacteriophage lambda and double-stranded DNA viruses in

36 We have used a set of bacteriophage lambda and Escherichia coli replication pr

37 infection of Escherichia coli bacteria with bacteriophage lambda and following the establishment of

38 Both bacteriophage lambda and herpes simplex virus (HSV) disp

39 situations: on the chromosome of E. coli, in bacteriophage lambda and in high-copy-number pUC-based p

40 The repressor of bacteriophage lambda and its homolog, LexA, preferential

41 hia coli strains expressing the red genes of bacteriophage lambda and lacking recBCD function either

42 y of peptides and proteins on the surface of bacteriophage lambda and to facilitate the use of modifi

43 I began with bacteriophage lambda and tried to establish the colinear

44 We used the recombination systems from bacteriophages lambda and P1 to engineer each strain in

45 degrading transglycosylases (endolysins) of bacteriophages lambda and P2, suggesting that the encode

46 The temperate bacteriophages lambda and P22 share similarities in thei

47 tained from pure samples of adenovirus, from bacteriophages lambda and T4 GT7, and from a mixture of

48 regulatory pause of the late gene operon of bacteriophage lambda, and that this process is enhanced

49 lity and induction of the lysogenic state of bacteriophage lambda are balanced by a complex regulator

50 s of the decision structure in the temperate bacteriophage lambda are consistent with our conclusions

51 e results to the process of DNA packaging in bacteriophage lambda are discussed.

52 iochemical studies on genome packaging using bacteriophage lambda as a model system.

53 g to elucidate the target-finding process in bacteriophage lambda as it infects an Escherichia coli c

54 , together with a complex functional role in bacteriophage lambda assembly, suggest that gpW has been

55 We follow the postinfection decision in bacteriophage lambda at single-virus resolution, and sho

56 ia include (a) Red recombination mediated by bacteriophage lambda, (b) integration of group II mobile

57 Bacteriophage lambda begins its infection cycle by eject

58 in of prophage HK022 excludes superinfecting bacteriophage lambda by blocking transcription elongatio

59 of hydrodynamically sheared genomic DNA from bacteriophage lambda can be amplified linearly.

60 s located downstream of the p(L) promoter of bacteriophage lambda can induce cell-cycle synchrony in

61 Herpes simplex type 1 virus (HSV-1) and bacteriophage lambda capsids undergo considerable struct

62 ical stability of gpD-free and gpD-decorated bacteriophage lambda capsids.

63 The integrase protein (Int) from bacteriophage lambda catalyzes the insertion and excisio

64 Crosses between a non-replicating linear bacteriophage lambda chromosome and a replicating plasmi

65 The cos site of the bacteriophage lambda chromosome contains the sites requi

66 -specific recombination pathway by which the bacteriophage lambda chromosome is excised from its Esch

67 eaction used to site-specifically excise the bacteriophage lambda chromosome out of its E. coli host

68 Bacteriophage lambda chromosomes are processively packag

69 nificantly lower than when a fragment of the bacteriophage lambda cI gene, also encoding a leaderless

70 exclusively on the isomerization step is the bacteriophage lambda cI protein (lambdacI), which has be

71 o different DNA-binding domains, that of the bacteriophage lambda cI protein and that of the Escheric

72 re, we use long-range gene regulation by the bacteriophage lambda CI protein as a powerful system to

73 We replaced the bacteriophage lambda cI repressor C-terminal dimerizatio

74 Cooperative binding of the bacteriophage lambda cI repressor dimer to specific site

75 rization via their abilities to dimerize the bacteriophage lambda cI repressor DNA-binding domain.

76 1 self-association was demonstrated by using bacteriophage lambda cI repressor fusion and pull-down a

77 An example is the bacteriophage lambda cI repressor, a dimeric protein tha

78 as an example the directed evolution of the bacteriophage lambda cI TF against two synthetic bidirec

79 witches for orthogonal logic gates, based on bacteriophage lambda cI variants and multi-input promote

80 The bacteriophage lambda CII protein stimulates the activity

81 ere used as a reagent to isolate recombinant bacteriophage lambda clones expressing antigens of the o

82 The rightward regulatory region of bacteriophage lambda contains two promoters, pRM and pR,

83 enerated all eight possible code variants of bacteriophage lambda Cro and used electrophoretic mobili

84 The overall affinity of the bacteriophage lambda Cro repressor for its operator DNA

85 merization and DNA binding equilibria of the bacteriophage lambda Cro repressor have been characteriz

86 l protein homodimers (bacteriophage N15 Cro, bacteriophage lambda Cro, and bacteriophage P22 Arc) wit

87 tions affects the lysis-lysogeny decision of bacteriophage lambda despite variable infection times be

88 The "genetic switch" of bacteriophage lambda determines whether a prophage stays

89 The p(R) and p(RM) promoters of bacteriophage lambda direct transcription in divergent d

90 plify 199- and 500-base pair (bp) regions of bacteriophage lambda DNA and 346- and 410-bp regions of

91 n-chip PCR/CE analysis of a 500-bp region of bacteriophage lambda DNA was also demonstrated.

92 t with these steady-state kinetic data, when bacteriophage lambda DNA was used as a substrate, maxima

93 required for site-specific recombination of bacteriophage lambda DNA.

94 cI repressors at the right operator (OR) of bacteriophage lambda DNA.

95 li, DnaK is essential for the replication of bacteriophage lambda DNA; this in vivo activity provides

96 Efficient expression of most bacteriophage lambda early genes depends upon the format

97 ichia coli by homologous recombination using bacteriophage lambda-encoded enzymes.

98 Such recombination is possible because bacteriophage lambda-encoded functions, called Red, effi

99 The bacteriophage lambda-encoded site-specific recombinase i

100 Bacteriophage lambda encodes a 28 kDa protein called bet

101 To counteract the latter activity, bacteriophage lambda encodes a small protein inhibitor c

102 To avoid this fate, bacteriophage lambda encodes the gene Gam whose product

103 We examined how a virus, bacteriophage lambda, evolved to infect its host, Escher

104 The bacteriophage lambda excisionase (Xis) protein is requir

105 digestion of lambda-phage DNA by individual bacteriophage lambda exonuclease molecules.

106 A bacteriophage lambda fragment and the Igf2r DMR2 showed

107 The orf gene of bacteriophage lambda, fused to a promoter, was placed in

108 Bacteriophage lambda gene Q protein and the related prot

109 The bacteriophage lambda genetic switch is still yielding su

110 ing, for example, derives in large part from bacteriophage lambda genetics.

111 n origin of replication and a segment of the bacteriophage lambda genome comprising the red genes (ex

112 ident below 6000 basepairs for unzipping the bacteriophage lambda-genome.

113 The CW02 capsid consists of bacteriophage lambda gpD-like densities that likely cont

114 Bacteriophage lambda had two distinct peaks in its size

115 The Cro protein from bacteriophage lambda has a dimeric alpha+beta fold that

116 Bacteriophage lambda has for many years been a model sys

117 Bacteriophage lambda has four adjacent genes -S, R, Rz a

118 The study of bacteriophage lambda has provided key insights into fund

119 Bacteriophage lambda has served as an ideal model system

120 their functional similarities to the lambda bacteriophage (lambda) holin.

121 influence the lysis-lysogeny decision of the bacteriophage lambda(imm434).

122 The structure of the Cro protein from bacteriophage lambda in complex with a 19 base-pair DNA

123 milar to the use of Escherichia coli DnaJ by bacteriophage lambda in DNA replication.

124 s the quiescent (lysogenic) transcriptome of bacteriophage lambda in infected Escherichia coli.

125 ia coli chromosomes bearing the red genes of bacteriophage lambda in place of recBCD was tested in st

126 s were screened for the ability to propagate bacteriophage lambda in the background of a dnaK deficie

127 sically disordered protein, the N protein of bacteriophage lambda, in the presence of high concentrat

128 is different from what occurs in the related bacteriophage lambda, in which binding of lambda repress

129 ted in the random lysis/lysogeny decision of bacteriophage-lambda, in the loss of synchrony of circad

130 Bacteriophage lambda infection of Escherichia coli can r

131 Bacteriophage lambda integrase (Int) catalyzes at least

132 Bacteriophage lambda integrase (Int) catalyzes site-spec

133 Bacteriophage lambda integrase (Int) catalyzes the integ

134 Bacteriophage lambda integrase (Int) is a versatile site

135 Site-specific recombination catalyzed by bacteriophage lambda integrase (Int) is essential for es

136 rformed by tyrosine recombinases such as the bacteriophage lambda Integrase (Int) protein.

137 ccumulation of natural Holliday junctions of bacteriophage lambda Integrase (Int)-mediated reactions.

138 ecombinase family of enzymes, which includes bacteriophage lambda Integrase (Int).

139 Bacteriophage lambda integrase (lambda-Int) is the proto

140 Bacteriophage lambda integrase (lambda-Int), a phage-enc

141 The bacteriophage lambda integrase catalyzes four site-speci

142 for site-specific recombination catalysed by bacteriophage Lambda Integrase protein (Int).

143 The bacteriophage lambda integrase protein (lambda Int) belo

144 cific recombination reaction mediated by the bacteriophage lambda integrase protein.

145 lexes similar to intasomes, which consist of bacteriophage lambda integrase, Escherichia coli integra

146 res similarities with the well-characterized bacteriophage lambda integration, Cre-lox is in many way

147 tor include partition of the cI repressor of bacteriophage lambda into two functional domains separat

148 The c1 repressor of bacteriophage lambda is a classic example of a protein t

149 The bacteriophage lambda is a convenient source of high qual

150 Bacteriophage lambda is a double-stranded DNA virus that

151 Bacteriophage lambda is a double-stranded DNA virus that

152 The site-specific recombinase (Int) of bacteriophage lambda is a heterobivalent DNA-binding pro

153 The site-specific recombinase (Int) of bacteriophage lambda is a heterobivalent DNA-binding pro

154 The N protein from bacteriophage lambda is a key regulator of transcription

155 Bacteriophage lambda is a paradigm for understanding the

156 The Q protein of bacteriophage lambda is a transcription antiterminator t

157 The cro repressor from bacteriophage lambda is an important and classical trans

158 Bacteriophage lambda is assembled from preformed viral c

159 Terminase from bacteriophage lambda is composed of gpA (72.4 kDa) and g

160 The terminase enzyme from bacteriophage lambda is composed of two viral proteins (

161 The lysogenic state of bacteriophage lambda is exceptionally stable yet the pro

162 The prophage state of bacteriophage lambda is extremely stable and is maintain

163 The lysogenic state of bacteriophage lambda is maintained by CI repressor, whic

164 Bacteriophage lambda is one of the most exhaustively stu

165 Bacteriophage lambda is one of the most extensively stud

166 The cosQ site of bacteriophage lambda is required for DNA packaging termi

167 The terminase enzyme from bacteriophage lambda is responsible for excision of a si

168 The terminase enzyme from bacteriophage lambda is responsible for the insertion of

169 The terminase enzyme from bacteriophage lambda is responsible for the insertion of

170 in the early part of the late gene operon of bacteriophage lambda is subject to such cleavage and res

171 The integrase protein (Int) from bacteriophage lambda is the archetypal member of the tyr

172 The excisionase (Xis) protein from bacteriophage lambda is the best characterized member of

173 A critical step in the assembly of bacteriophage lambda is the excision of a single genome

174 an enzyme encoded by the Nu1 and A genes of bacteriophage lambda, is crucial for packaging concateme

175 he bacterium Escherichia coli and its virus, bacteriophage lambda, is paradigmatic for gene regulatio

176 Bacteriophage lambda lacking its Red recombination funct

177 sitive control" mutants of the cI protein of bacteriophage lambda (lambda cI) bind DNA but, unlike th

178 The protein phosphatase encoded by bacteriophage lambda (lambda PP) belongs to a family of

179 We also show that SarA binds to the bacteriophage lambda (lambda) attachment site, attL, pro

180 Bacteriophage lambda (lambda) permits the display of man

181 The bacteriophage lambda (lambda) recombination system Red h

182 sical and functional characterization of the bacteriophage lambda (lambda) scaffolding protein gpNu3.

183 The site-specific recombinase encoded by bacteriophage lambda [lambda Integrase (Int)] is respons

184 The cI protein of bacteriophage lambda (lambdacI) activates transcription

185 The cI protein of bacteriophage lambda (lambdacI) activates transcription

186 The Q protein of bacteriophage lambda (lambdaQ) is a transcription anti-t

187 the filtrates of M. tuberculosis cultures, a bacteriophage lambda library of M. tuberculosis H37Rv DN

188 Upon induction of a bacteriophage lambda lysogen, a site-specific recombinat

189 ent phenanthriplatin on Escherichia coli and bacteriophage lambda lysogens is reported.

190 Bacteriophage lambda makes two proteins with overlapping

191 The integrase protein of bacteriophage lambda mediates recombination via four dis

192 In the bacteriophage lambda model for site-specific recombinati

193 es to survey the roles of P-loop residues in bacteriophage lambda motor function.

194 Bacteriophage lambda moves its viral genome into and out

195 Bacteriophage lambda N and bacterial Nus proteins togeth

196 enhanced affinity and specificity using the bacteriophage lambda N peptide-boxB interaction as a mod

197 Transcription antitermination by the bacteriophage lambda N protein is stimulated in vitro by

198 The bacteriophage lambda N protein reduces transcriptional s

199 orter system based on antitermination by the bacteriophage lambda N protein, it has been possible to

200 al positive regulators of gene expression is bacteriophage lambda N protein.

201 does not extend to another ClpXP substrate, bacteriophage lambda O protein, suggesting that RssB act

202 e-specific DNA-binding proteins, such as the bacteriophage lambda O replication initiator or the E. c

203 Bacteriophage lambda of Escherichia coli has two alterna

204 mutations in the large terminase subunit in bacteriophage lambda on packaging motor dynamics.

205 The effects of Kil peptide from bacteriophage lambda on the assembly of Escherichia coli

206 d cosmid vector pLorist6Xh, which contains a bacteriophage lambda origin of replication for low-copy-

207 ein, which is critical for lysogenization by bacteriophage lambda, overlaps the -35 region of the P(R

208 The bacteriophage lambda P and Escherichia coli DnaC protein

209 cles in the lysogeny maintenance promoter of bacteriophage lambda, P(RM).

210 N proteins from bacteriophages lambda, P22, and phi21 modulate transcrip

211 containing the major Xis binding sites from bacteriophages lambda, P22, L5, HP1, and P2 and the conj

212 Rapid ejection of a 0.3(+) T7 genome from a bacteriophage lambda particle results in degradation of

213 lophosphoesterase superfamily exemplified by bacteriophage lambda phosphatase (lambda-Pase).

214 metallo-phosphoesterase fold (exemplified by bacteriophage lambda phosphatase) embellished by distinc

215 Bacteriophage lambda phosphoprotein phosphatase (lambdaP

216 formed on initiation complexes formed on the bacteriophage lambda PR promoter.

217 relief of TI by CI or Cro repressors in the bacteriophage lambda PR-PRE system show strong relief of

218 beta protein from bacteriophage lambda promotes a single-strand annealing

219 te-specific recombinase integrase encoded by bacteriophage lambda promotes integration and excision o

220 Bacteriophage lambda protein phosphatase (lambdaPP) is a

221 Bacteriophage lambda protein phosphatase (lambdaPP) is a

222 Bacteriophage lambda protein phosphatase (lambdaPP) with

223 Reconstitution of bacteriophage lambda protein phosphatase in the presence

224 Bacteriophage lambda protein phosphatase is also conside

225 The interaction of bacteriophage lambda protein phosphatase with Mn2+ was s

226 an asymmetrical folding transition state of bacteriophage lambda protein W, which has yet to be subj

227 4/beta-flap interaction is required for the bacteriophage lambda Q antiterminator protein (lambdaQ)

228 main of the beta subunit is required for the bacteriophage lambda Q antiterminator protein to contact

229 Bacteriophage lambda Q-protein stably binds and modifies

230 The bacteriophage lambda recombination system has proven to

231 ar mechanistic differences between Tn916 and bacteriophage lambda recombination.

232 hat process the recombining oligo and affect bacteriophage lambda Red-mediated oligo recombination.

233 Here, we used bacteriophage lambda Red-mediated recombination, or "rec

234 The system utilizes the well defined bacteriophage lambda regulatory circuit where the viral

235 The bacteriophage lambda relies on interactions of the cI an

236 Bacteriophage lambda repressor activates transcription f

237 The bacteriophage lambda repressor and its relatives bind co

238 MalK was also demonstrated in vivo using the bacteriophage lambda repressor fusion assay.

239 lcB polypeptide to the DNA-binding domain of bacteriophage lambda repressor leads to the formation of

240 s used to measure the oligomerization of the bacteriophage lambda repressor protein at micromolar con

241 Our data show that unlike bacteriophage lambda, repressor bound at O(L) of bacteri

242 stigated the binding interaction between the bacteriophage lambda-repressor CI and its target DNA usi

243 Under usual laboratory conditions, lysis by bacteriophage lambda requires only the holin and endolys

244 recently been identified as the factor from bacteriophage lambda responsible for the inhibition of b

245 hia coli with the red recombination genes of bacteriophage lambda results in a strain in which adapti

246 Bacteriophage lambda's N-protein includes a 17-amino-aci

247 ouble-stranded DNA from the Escherichia coli bacteriophage lambda served as the model DNA in our expe

248 The life cycle of bacteriophage lambda serves as a simplified paradigm for

249 manner similar to the excisionase protein of bacteriophage lambda, serving an architectural role in t

250 The bacteriophage lambda site-specific recombinase (Int), in

251 n Fis was previously shown to be involved in bacteriophage lambda site-specific recombination in vivo

252 Bacteriophage lambda site-specific recombination is cata

253 Bacteriophage lambda site-specific recombination require

254 ing of target sequences by making use of the bacteriophage lambda site-specific recombination system.

255 Bacteriophage lambda stably maintains its dormant propha

256 Several features of bacteriophage lambda suit it for the study of genetic re

257 Thus, the bacteriophage lambda surface display is powerful for iso

258 Bacteriophage lambda surface display was used to isolate

259 A bacteriophage lambda surface expression system, lambda f

260 und in specialized gene circuits such as the bacteriophage lambda switch and the Cyanobacteria circad

261 argeting various positions in the genomes of bacteriophages lambda, T5, T7, T4 and R1-37 and investig

262 Using fluorescently labelled bacteriophage lambda tails, we quantitatively described

263 Bacteriophage lambda terminase holoenzyme is a hetero-ol

264 Bacteriophage lambda terminase is composed of two subuni

265 Bacteriophage lambda terminase possesses a site-specific

266 The DNA packaging enzyme of bacteriophage lambda, terminase, is a heteromultimer com

267 Bacteriophage lambda-terminase, which serves as a protot

268 Terminase is an enzyme from bacteriophage lambda that is required for insertion of t

269 component of the Red recombination system of bacteriophage lambda that promotes a single strand annea

270 In bacteriophage lambda the catalytic terminase subunit is

271 In bacteriophage lambda, the DNA recognition protein is gpN

272 In the particular case of bacteriophage lambda, the ejection is 50% inhibited by o

273 In bacteriophage lambda, the overlapping open reading frame

274 In bacteriophage lambda, this frameshift controls productio

275 l-characterized (lambda)Xis excisionase from bacteriophage lambda, Tn916Xis stimulates excision in vi

276 mbinant plasmid, as judged by the ability of bacteriophage lambda to form plaques, indicating that th

277 newly replicated chromosomes, and is used by bacteriophage lambda to integrate or excise its genome i

278 elting, and variants of the P(R) promoter of bacteriophage lambda to model the closed complex interme

279 ing of 'decoration' proteins (such as gpD in bacteriophage lambda) to the viral shell.

280 e in terminating transcripts in vitro at the bacteriophage lambda tR1 terminator and had correspondin

281 The bacteriophage lambda tR1 terminator encodes a region of

282 xpress the HBV X protein (HBx) and possess a bacteriophage lambda transgene to evaluate the in vivo e

283 ansgenic mice that express HBx and possess a bacteriophage lambda transgene were sacrificed at 30, 90

284 at both express HBx (ATX mice) and possess a bacteriophage lambda transgene with the hepatocarcinogen

285 en evaluated by genetic footprinting using a bacteriophage lambda transposon delivery system.

286 fic recombination reactions catalyzed by the bacteriophage lambda tyrosine recombinase integrase (Int

287 Bacteriophage lambda uses a holin-endolysin system for h

288 transcription antiterminator N protein from bacteriophage lambda uses its arginine-rich motif to spe

289 Bacteriophage lambda uses site-specific recombination to

290 nsgenic fish that carry multiple copies of a bacteriophage lambda vector that harbors the cII gene as

291 SSA utilizes tagged retroviral libraries in bacteriophage lambda vectors (retrophages).

292 igenic structures in aqueous preparations of bacteriophage lambda, Venezuelan equine encephalitis vir

293 ightly regulated pathway for the excision of bacteriophage lambda viral DNA out of the E. coli host c

294 The attachment site (attlambda) of bacteriophage lambda was examined in wild strains of Esc

295 The rap gene of bacteriophage lambda was placed in the chromosome of an

296 pressure on the extent of DNA ejection from bacteriophage-lambda was recently investigated.

297 For bacteriophage lambda, we establish a mean ejection time

298 was found by measuring the size spectrum of bacteriophage lambda, which has a noncontractile tail.

299 F) is a protein that binds to the H' site of bacteriophage lambda with sequence specificity.

300 hich a whole genome library is cloned into a bacteriophage lambda ZAP Express vector which contains b