ライフサイエンスコーパス: basal body

1 ed correlated fluctuations of the cilium and basal body.

2 rsion of the mother centriole into the cilia basal body.

3 tes proteasomal activity specifically at the basal body.

4 component macromolecular machine we call the basal body.

5 te protein Cep290 to both satellites and the basal body.

6 that Gle1 is enriched at the centrosome and basal body.

7 in in the region of the CP that contains the basal body.

8 vated Dishevelled-1 (Dvl-1) localized to the basal body.

9 alizing to a polar structure adjacent to the basal body.

10 d with the loss of Dvl-1 localization to the basal body.

11 d from the cytoplasm by the ciliary neck and basal body.

12 omponents participate in the assembly of the basal body.

13 the apical cell surface associated with the basal body.

14 n complexes at the cilia transition zone and basal body.

15 referentially near the posterior face of the basal body.

16 otein, which is located around the flagellar basal body.

17 1 is specifically expressed in photoreceptor basal body.

18 around an effective hinge located below the basal body.

19 ated Ca(2+)-binding proteins associated with basal bodies.

20 nction as microtubule-organizing centers and basal bodies.

21 is in directing the orientation of cilia and basal bodies.

22 n is crucial for the recruitment of TTBK2 to basal bodies.

23 m to be necessary for the patterning of cone basal bodies.

24 scale centriole amplification that generates basal bodies.

25 and a pattern of growth common to euconodont basal bodies.

26 e flagellar pocket collar and bi-lobe to the basal bodies.

27 pulation (E2) with only two cilia and unique basal bodies.

28 smic protein that co-purifies with flagellar basal bodies.

29 ojections that assemble on centriole-derived basal bodies.

30 established roles in the assembly of nascent basal bodies.

31 complement of Bld10 is required to stabilize basal bodies.

32 etric mechanical forces that are resisted by basal bodies.

33 evented the association of Inturned with the basal bodies.

34 ures demonstrates a marked compaction of the basal body (4 nm) occurs when the needle tip contacts th

35 were localized to the flagellar pocket, the basal bodies, a tripartite attachment complex linking th

36 Each flagellum consists of a basal body, a hook, and a filament.

37 uctural foundation of the injectisome is the basal body, a molecular lock-nut structure composed pred

38 Bld10 protein continues to accumulate at basal bodies after assembly, and we hypothesize that the

39 lular mechanism regulated the positioning of basal bodies after the transition to the adult mosaic an

40 bule glutamylation are required to stabilize basal bodies against ciliary beating forces.

41 acts to maintain the structural integrity of basal bodies against the forces of ciliary beating in ad

42 mylation and Bld10, Poc1, and Fop1 stabilize basal bodies against the forces produced by ciliary beat

43 om the basal-foot cap, together with loss of basal-body alignment and cilium orientation, defects in

44 e, Rpgrip1l was required for positioning the basal body along the planar polarity axis.

45 III secretion apparatus (T3SA) consists of a basal body, an extracellular needle, and a tip complex t

46 Basal bodies and centrioles play central roles in microt

47 ed, planar polarized MTs that originate from basal bodies and interact, via their plus ends, with mem

48 ed-coil protein that localizes to centrioles/basal bodies and plays a crucial role in the formation a

49 how TbRP2 is targeted and tethered at mature basal bodies and provide novel insight into TbRP2 functi

50 Cp110 localizes to cilia-forming basal bodies and rootlets, and is required for ciliary a

51 stead that pore proteins localize to ciliary basal bodies and that their perturbation leads to congen

52 esis due to impaired association between the basal bodies and the actin cytoskeleton, suggesting that

53 t that both mutants had wild type numbers of basal bodies and the flagellar motors were functional.

54 al adhesion (FA) proteins associate with the basal bodies and their striated rootlets and form comple

55 elated to the cell cycle and to flagella and basal bodies and to assign isoforms of duplicated enzyme

56 is cell assembles approximately 26 flagellar basal bodies and we show that basal body number is contr

57 bs a variety of signaling pathways along its basal body and axoneme that are critical for embryonic d

58 at the transition zone, located between the basal body and axoneme, to regulate the localization of

59 n is the transition zone, which lies between basal body and axoneme.

60 nexpected findings include the following: 1) basal body and bilobe duplication are concurrent; 2) mat

61 Here we report that Fuzzy localizes to the basal body and ciliary axoneme and is essential for cili

62 MARK4 associated with the basal body and ciliary axoneme in human and murine cell

63 In the mouse retina, ARL2BP localized to the basal body and cilium-associated centriole of photorecep

64 ting it to Rab8-positive vesicles and to the basal body and cilium.

65 Meckelin localizes to the primary cilium, basal body and elsewhere within the cell.

66 ved loss of attachments between the nucleus, basal body and mitochondria.

67 We show that CEP41 is localized to the basal body and primary cilia, and regulates ciliary entr

68 l for ciliogenesis by delivering Rab8 to the basal body and primary cilium.

69 lla is a structural intermediate between the basal body and the axoneme that regulates ciliary traffi

70 oreover, RNAi of SPBB1 confined TbPLK at the basal body and the bilobe structure, resulting in consti

71 n addition, AEE788 blocks duplication of the basal body and the bilobe without affecting mitosis.

72 SPBB1 localizes to both the mature basal body and the probasal body throughout the cell cyc

73 assembly is triggered by mature centrioles (basal bodies) and requires centrosomal protein 164kDa (C

74 egulated early, when most cells were forming basal bodies, and 73 genes genes upregulated late, when

75 izes to centrioles and to the distal ends of basal bodies, and interacts with septin2, a protein impl

76 The asymmetric positioning of basal bodies, and therefore cilia, is often critical for

77 gular deposition of proteins on spermatocyte basal bodies, and, consequently, distorted axonemal asse

78 a large cytoplasmic complex, a transmembrane basal body, and an extracellular needle.

79 le docks with the plasma membrane, becomes a basal body, and grows a cilia bud that we call a procili

80 d by localization of TbSAS6 at newly forming basal bodies; and 3) kinetoplast division is observed in

81 de-gated (CNG) channel, as well as disrupted basal body apical migration in postnatal olfactory senso

82 Basal bodies are assembled rapidly (< 5 min) but the ass

83 on tomography on mouse trachea, we show that basal bodies are collectively hooked at the cortex by a

84 3a function precedes ciliogenesis as ciliary basal bodies are mispositioned in mutant photoreceptors.

85 We conclude that pro-centrioles/pro-basal bodies are multipotent and not committed to form e

86 We find that basal bodies are not positioned randomly on the cell sur

87 Basal bodies are radially symmetric, microtubule-rich st

88 on, specific triplet microtubules within the basal body are more susceptible to loss, probably due to

89 ctural genes required for the flagellar hook-basal body are required for robust activation of sigma(D

90 the cilium and its anchoring structure, the basal body, are the major contributors to both disease c

91 e in the recruitment of Ttbk2 to the ciliary basal body as well as the removal of Cp110 from the cili

92 y TbRP2 recruitment, is coupled with nascent basal body assembly, monitored by localization of TbSAS6

93 beating in addition to its separable role in basal body assembly.

94 Chibby (Cby), a basal-body associated protein, regulates beta-catenin-me

95 d microtubule glutamylation incorporate into basal bodies at distinct stages of assembly, culminating

96 ctions as a scaffold to stabilize the entire basal body barrel.

97 ized at a different axial level bridging the basal body (BB) and other TZ proteins.

98 Planar cell polarity (PCP) regulates basal body (BB) docking and positioning during cilia for

99 surface is determined by the location of the basal body (BB) that templates the cilium.

100 is nucleated by the mother centriole-derived basal body (BB) via as yet poorly characterized mechanis

101 Cilia-organizing basal bodies (BBs) are microtubule scaffolds that are vi

102 nt the MT and actin-based network of ciliary basal bodies below the apical surface.

103 kinetoplast DNA), duplication of organelles (basal body, bilobe, kinetoplast, nucleus), and cytokines

104 f a membranous protrusion extending from the basal body but lacking axonemal structure and remains un

105 Root is required for cohesion of basal bodies, but the cilium structure appears normal in

106 e five centrins associate with the flagellar basal body, but no centrin has been found to regulate fl

107 Bld10 stabilizes basal bodies by promoting the stability of the A- and C-

108 d10/Cep135 in basal body maintenance so that basal bodies can withstand the forces produced by motile

109 actor C domain-containing protein TbRP2 is a basal body (centriolar) protein essential for axoneme fo

110 ein, a truncated paralogue of the ubiquitous basal body/centriole protein SAS6, has been characterise

111 oreceptor markers and exhibited axonemes and basal bodies characteristic of outer segments.

112 tion, we find that Presenilin-2 localizes to basal bodies/cilia through a conserved VxPx motif.

113 om its characterised roles at the centrosome/basal body/cilia network.

114 endent polarized exocytosis acts through the basal body-ciliary complex to spatially regulate Notch s

115 Basal body/ciliary perturbation in murine pancreatic isl

116 early simultaneously, and that refinement of basal body/cilium orientation continues during airway ep

117 nced by, but does not strictly require, hook-basal body completion and instead demands a minimal subs

118 Mice lacking the basal body component Chibby (Cby) exhibit impaired mucoc

119 ether, these results suggest that bbof1 is a basal body component required in MCCs to align and maint

120 ed into several families based on structural basal body components.

121 Removal of galectin-3, one of basal-body components, provokes misrecruitment of gamma-

122 Analyses of basal body composition in different species suggest that

123 Basal bodies comprise nine symmetric triplet microtubule

124 Centriole-to-basal body conversion, a complex process essential for c

125 ted FlaH binding to FlaI form the archaellar basal body core.

126 diffusion delays return of kinesin-II to the basal body, depleting kinesin-II available for anterogra

127 These sperm centrioles appear as vestigial basal bodies, destroyed in the mid-to-lower corpus.

128 a serovar Typhimurium pathogenicity island 1 basal body, determined using single-particle cryo-electr

129 forces generated by ciliary beating promote basal body disassembly in bld10Delta cells.

130 deficient in filamentary connections between basal bodies display markedly different synchronization

131 We find that basal bodies dock after polarity of PCP proteins is esta

132 n centrosome polarization at the synapse and basal body docking during ciliogenesis [1, 4-8], suggest

133 y regulator of ciliary vesicle formation and basal body docking during the differentiation of airway

134 kif3a is dispensable for basal body docking in otic vesicle sensory epithelia and

135 ding, but not a global loss of ciliogenesis, basal body docking or PCP signaling leads to dysfunction

136 ithelial cells, we show that Cby facilitates basal body docking to the apical cell membrane through p

137 t the distal end of centrioles to facilitate basal body docking to the plasma membrane.

138 npoint TAp73 as necessary and sufficient for basal body docking, axonemal extension, and motility dur

139 gulates centriole duplication in metazoa and basal body duplication in flagellated and ciliated organ

140 hat actin plays a role in IFT recruitment to basal bodies during flagellar elongation and that when a

141 robably due to force distribution within the basal body during ciliary beating.

142 ll cycle, and co-localizes with TbPLK at the basal body during early cell cycle stages.

143 t Bld10 protein first incorporates stably at basal bodies early during new assembly.

144 (emb)Kif3a(-/-) and in (emb)Ift88(-/-) mice, basal bodies failed to extend transition zones (connecti

145 ion between the cell cycle and its centriole-basal body-flagellar cycle.

146 We also demonstrate that basal body formation in the male testes and the producti

147 entiates ciliogenesis, but the regulation of basal body formation is not fully understood.

148 Furthermore, pro-basal bodies formed under conditions conducive for 9+2 a

149 conversion of the mother centriole into the basal body, from which axonemal microtubules extend to f

150 ctions associated with the centriole-derived basal body, from which axonemal microtubules grow and wh

151 tly, we show that Asl is required for proper basal body function and spermatid axoneme formation.

152 centrin 2 homologue, is required for proper basal body function.

153 d beta-cells along with misregulated ciliary/basal body gene expression in pancreatic islets in a dia

154 ns of the centriole, the centrosome, and the basal body have an impact upon many aspects of developme

155 ubtilis by fluorescently labelling flagellar basal bodies, hooks and filaments.

156 c novel component is linked to the flagellar basal body; however, nothing is known about the proteins

157 y expressed in cells that require functional basal bodies, i.e., sensory neurons and male germ cells.

158 erved component of centrioles in animals and basal bodies in flagellated organisms.

159 WDR34 concentrates around the centrioles and basal bodies in mammalian cells, also showing axonemal s

160 osome and transition zone marker, identified basal bodies in Nphp5(-/-) photoreceptors, but without f

161 s establish the asymmetrical localization of basal bodies in red-, green-, and blue-sensitive cones i

162 d concentrates at the centrosome and ciliary basal body in addition to the nucleus in interphase cell

163 We show here that AURKA activation at the basal body in ciliary disassembly requires interactions

164 aused the loss of ARL2BP localization at the basal body in ciliated nasal epithelial cells.

165 ns of the centriole, the centrosome, and the basal body in different tissues and cultured cells of Dr

166 nstrate KIAA0586 protein localization at the basal body in human and mouse photoreceptors, as is comm

167 tion of the maturation of a second flagellar basal body in late G1 phase, DNA replication in S phase,

168 ct--PtdIns(4)P--accumulate at the centrosome/basal body in non-ciliated, but not ciliated, cells.

169 racterization of the major components of the basal body in the assembled state, including that of the

170 cilium of stimulated beta-cells and ciliary/basal body integrity is required for activation of downs

171 to how the ciliopathy protein Poc1 maintains basal body integrity.

172 The recruitment of IFT components to basal bodies is a function of flagellar length, with inc

173 estor served as the rootlet of the flagellar basal bodies is required for parasite cell division.

174 The centriole/basal body is a eukaryotic organelle that plays essentia

175 ted cells segregation of the organelles i.e. basal bodies, kinetoplast and nucleus was disrupted.

176 till localizes to intercellular borders, but basal body localization is lost.

177 We show that basal body localization of TbRP2 is mediated by twinned,

178 small GTPase ARF4 is required for Presenilin basal body localization, Notch signaling, and subsequent

179 Among these, a role for basal body-localized AURKA in regulating ciliary disasse

180 ding a large inner circular structure in the basal body lumen, which functions as a scaffold to stabi

181 entify a novel mechanism for Bld10/Cep135 in basal body maintenance so that basal bodies can withstan

182 in cilia length and number, due to defective basal body maturation and apical docking.

183 cient MCCs restored ciliogenesis by rescuing basal body maturation and docking.

184 d cell surface projections that emanate from basal bodies, membrane-docked centrioles.

185 let microtubules, suggesting that the mother basal body microtubule structure does not template the d

186 cilia formation defects due to impairment of basal body migration and docking.

187 se in IFT mutants, which display much weaker basal body mispositioning.

188 h to monitor the movements of the kinocilium basal body (mother centriole) and its daughter centriole

189 aft that couples the torque generated by the basal body motor to the extracellular hook and filament.

190 cells were enlarged in size with duplicated basal bodies, multinuclei and new flagella that are deta

191 As the anchor for motile cilia, basal bodies must be resistant to the forces directed to

192 Basal bodies nucleate, anchor, and organize cilia.

193 y 26 flagellar basal bodies and we show that basal body number is controlled by SwrA.

194 Rather, basal bodies occupy a grid-like pattern organized symmet

195 Unexpectedly, basal bodies of injectisomes inside the bacterial cells

196 L, have aberrant localization of AHI1 at the basal bodies of PC and at cell-cell junctions, likely th

197 M92A colocalizes with Cby1 at the centrioles/basal bodies of primary cilia, while FAM92B is undetecta

198 We report that, in the adult retina, the basal bodies of red-, green-, and blue-sensitive cone ph

199 greater than 100 centrioles, which form the basal bodies of their motile cilia.

200 In contrast, no patterning was seen in the basal bodies of ultraviolet-sensitive cones or in rod ph

201 lls; IFT components are not recruited to the basal body of cilia.

202 We show that Pk3 is enriched at the basal body of GRP cells but is recruited by Vangl2 to an

203 dition, antibodies against TTLL5 stained the basal body of photoreceptor cells in rat and the centros

204 We show that SSX2IP localizes to the basal body of primary cilia in human and murine ciliated

205 tor, Cdc20, is specifically recruited to the basal body of primary cilia.

206 e we describe localization of SDCCAG3 to the basal body of primary cilia.

207 nohistology revealed KIZ localization at the basal body of the cilia in human fibroblasts, thus shedd

208 We show that ARMC9 localizes to the basal body of the cilium and is upregulated during cilio

209 the single-unit mitochondrial genome to the basal body of the flagellum and mediates the segregation

210 the linkage of the mitochondrial DNA to the basal body of the flagellum.

211 cular plate (CP), and they also encircle the basal body of the kinocilium.

212 sults show that the anchoring effects of the basal body on the cilium axoneme behave as a nonlinear r

213 called the BBSome which is localized at the basal body or ciliary axoneme and regulates the ciliary

214 ion to cell polarity, PCP components control basal body organization and function.

215 suggesting that Pk3 has a novel function in basal body organization.

216 homologue, has functions similar to Cen1 in basal body orientation, maintenance, and separation.

217 Bld10/Cep135 is a basal body outer cartwheel domain protein that has estab

218 hrough which the microtubule doublets of the basal body pass.

219 Basal body patterning was unaffected in the cones of the

220 ensity of multiciliated cells, the number of basal bodies per multiciliated cell, and the numbers of

221 Despite the severity of their basal body phenotype, kif3a mutant photoreceptors surviv

222 such as number control, the coordination of basal body placement and the regulation of beat patterns

223 sory microtubule structures that extend from basal bodies, plasma membrane-docked mother centrioles.

224 Basal body position is genetically determined by FlhF an

225 cal to those caused by meckelin loss, namely basal body positioning and ciliogenesis defects.

226 ng the length of the FAZ filament to control basal body positioning and life cycle transitions in T.

227 n 16 flagella, we find diverse symmetries of basal body positioning and of the flagellar apparatus th

228 in the normal processes of ciliogenesis and basal body positioning.

229 Taken together, our data indicate that basal body proteasomal regulation governs paracrine sign

230 Altogether, these results identified a basal body protein as a TbPLK substrate and its essentia

231 quitous but functionally enigmatic flagellar basal body protein FliL is involved in P. mirabilis surf

232 Here we demonstrate that the centriolar and basal body protein HYLS-1, the C. elegans orthologue of

233 Mutations in the basal body protein MKS1 account for 7% of cases of MKS.

234 The evolutionarily conserved centriole/basal body protein SAS-4 regulates centriole duplication

235 omes in neuroblasts, both requiring Bld10, a basal body protein with varied functions.

236 agellar proteins that includes the FliF/FliG basal body proteins, the flagellar type III export appar

237 hat a specialized autophagy pathway near the basal body regulates cilium assembly.

238 ver, the molecular components that stabilize basal bodies remain poorly defined.

239 anisms that contribute to the maintenance of basal bodies remain to be discovered.

240 d flagellum and repositioned kinetoplast and basal body, reminiscent of epimastigote-like morphology.

241 These results indicate that mammalian basal body replication and ciliogenesis occur independen

242 eukaryotes, Centrin2 (CETN2) is required for basal body replication and positioning, although its fun

243 Our data suggest that basal bodies require a centrin from both groups in order

244 on by the actin cytoskeleton surrounding the basal body results in active ciliary movement.

245 , including cell cycle control, flagella and basal bodies, ribosome biogenesis, and energy metabolism

246 in is secreted in response to flagellar hook-basal body secretion and can be used as a secretion sign

247 ubstrate and its essential role in promoting basal body segregation and flagellum attachment zone fil

248 st SPBB1 in procyclic trypanosomes inhibited basal body segregation, disrupted the new flagellum atta

249 n Trypanosoma brucei plays multiple roles in basal body segregation, flagellum attachment, and cytoki

250 cells display cilia with large, star-shaped basal bodies, similar to the Ecc cells described for the

251 ype III secretion apparatus is composed of a basal body spanning both bacterial membranes and an expo

252 functionally interacts with the established basal body stability components Bld10 and Poc1.

253 ass flagellar gene expression until the hook-basal body structural intermediate is completed and FlgM

254 Hence, CBY is essential for normal basal body structure and function in Drosophila, potenti

255 sis and chloroplast biogenesis, flagella and basal body structure/function, cell growth and division,

256 as significantly reduced at (tam)Arl13b(-/-) basal bodies, suggesting impairment of intraflagellar tr

257 distribution between cytoplasm and flagellar basal bodies, suggesting that FlhG effects flagellar loc

258 longitudinal structural variations along the basal body, suggesting a sequential and coordinated asse

259 ARL3, caused displacement of ARL2BP from the basal body, suggesting that ARL2 is vital for recruiting

260 ulin and Nedd1 no longer associated with the basal body, suggesting that Pk3 has a novel function in

261 We found that the Ppt1-KO mice had lower basal body temperature as they aged and developed hypoth

262 iscovered that TRPV1 receptors also regulate basal body temperature in multiple species from mice to

263 TRPV1 KO mice have a normal basal body temperature, indicative of developmental comp

264 Despite a normal basal body template, Ttbk2 mutants lack cilia.

265 Basal bodies that are missing triplets likely remain com

266 ly of small Ca(2+)-binding proteins found at basal bodies that are placed into two groups based on se

267 other cytoskeletal components including the basal bodies that seed the flagellum and the flagellar p

268 III secretion apparatus (T3SA) consists of a basal body that spans the bacterial envelope and an expo

269 These include the basal bodies, the bilobe, and the flagellar attachment z

270 ter FAZ, accompanied by repositioning of the basal body, the kinetoplast, Golgi, and flagellar pocket

271 s the core of the centrosome but also as the basal body, the structure that templates the formation o

272 The ciliopathy protein Poc1 stabilizes basal bodies through an unknown mechanism.

273 GCP2-GCP4, and is primarily localized in the basal body throughout the cell cycle.

274 ation of spindle poles in mitosis and act as basal bodies to assemble primary cilia in interphase.

275 l13b(-/-) photoreceptors revealed docking of basal bodies to cell membranes, but mature transition zo

276 ization of microtubules that polymerize from basal bodies to form the axoneme, which consists of hund

277 nt fashion and are responsible for anchoring basal bodies to the actin cytoskeleton during ciliogenes

278 a tripartite attachment complex linking the basal bodies to the kinetoplast, and a segment of microt

279 show that CP110 is required for anchoring of basal bodies to the membrane during cilia formation.

280 er these proteins help connect the flagellar basal body to the peptidoglycan wall, we surveyed a set

281 d transport system that moves cargo from the basal body to the tip of flagella [1].

282 Upon cell cycle exit, centriole-to-basal body transition facilitates cilia formation.

283 CBY was associated with the basal body transition zone (TZ) in these two cell types.

284 Here, we report the structure of the basal body triplet at 33 A resolution obtained by electr

285 the stability of the A- and C-tubules of the basal body triplet microtubules and by properly position

286 Our data indicate that Poc1 stabilizes basal body triplet microtubules through linkers between

287 ell as the removal of Cp110 from the ciliary basal body, two critical steps in initiating ciliogenesi

288 st these forces, distinct regions within the basal body ultrastructure and the microtubules themselve

289 show that CYLD localizes at centrosomes and basal bodies via interaction with the centrosomal protei

290 The asymmetric localization of basal bodies was consistent in all regions of the adult

291 2 cells of the lateral ventricles, but their basal bodies were different from those of E2 or E1 cells

292 en in 7-days-postfertilization (dpf) larvae; basal bodies were randomly distributed in all the photor

293 tood ciliopathy-associated protein Jbts17 at basal bodies, where they act to recruit a specific subse

294 We also found that Dlg5 is localized at the basal body, where it associates with another pathway com

295 at cell-cycle regulators target TTBK2 to the basal body, where it modifies specific targets to initia

296 TTBK2 acts at the distal end of the basal body, where it promotes the removal of CP110, whic

297 1 localizes to the centrosome and/or ciliary basal body, whereas defective TAPT1 mislocalizes to the

298 face, and for the rotation of the duplicated basal bodies, which positions the new flagellum so that

299 -tubulin kinase-2 (TTBK2) recruitment to the basal body, which promotes the removal of microtubule ca

300 lets likely remain competent to assemble new basal bodies with nine triplet microtubules, suggesting