ライフサイエンスコーパス: basal promoter

1 taining the Gal4 binding sites upstream of a basal promoter.

2 region between the vitamin D(3) enhancer and basal promoter.

3 tical regulatory regions for the human NAG-1 basal promoter.

4 both orientations and at a distance from the basal promoter.

5 This repression was targeted to the basal promoter.

6 ld enhance luciferase expression driven by a basal promoter.

7 re expression when fused to a nonfunctioning basal promoter.

8 hree p53 DNA-binding sites but no additional basal promoter.

9 lement and over the proximal tissue-specific basal promoter.

10 TFII-I on the TATA box containing the c-fos basal promoter.

11 inhibition is achieved by suppression of the basal promoter.

12 pstream promoter or the first intron to this basal promoter.

13 ors SRC-1 and SRC-2 to the distal region and basal promoter.

14 en multimerized and linked to a heterologous basal promoter.

15 ssion when placed upstream of a heterologous basal promoter.

16 of the INS-VNTR by AIRE requires the insulin basal promoter.

17 ptional machinery to the chromatin-repressed basal promoter.

18 d by sequences within 300 bp upstream of the basal promoter.

19 tively acting upstream elements close to the basal promoter.

20 imately 135 nucleotides that was active as a basal promoter.

21 l types and in the context of a heterologous basal promoter.

22 cis-regulating sequence upstream of the CoAA basal promoter.

23 required for maintaining the activity of the basal promoter.

24 ent (TRE) approximately 1 kb upstream of the basal promoter.

25 lled by an enhancer that lies -3 kb from the basal promoter.

26 ssential for maintaining the activity of the basal promoter.

27 of acting over several kilobases to silence basal promoters.

28 essors act over several kilobases to silence basal promoters.

29 tin structure and a consequent activation of basal promoters.

30 lobal transcription regulator acting through basal promoters.

31 ribution of Htz1 from activated to repressed/basal promoters.

32 middle region upstream of p109 or a CaMV 35S basal promoter (-64 to +6) were fused to gus.

33 The basal promoter (-65/+11) lacks cell-type specificity, wh

34 ranscriptional activity driven by the PDGF-A basal promoter (-82 to +8).

35 For the hRFC-A basal promoter, a CRE/AP-1-like element was bound by the

36 es which do not express ARPP-21 identified a basal promoter active in both neuronal and non-neuronal

37 ncated construct (+1 to -99) functioned as a basal promoter active in leukocytes and fibroblasts.

38 transfected with alpha5 integrin resulted in basal promoter activities 5-10-fold higher than those of

39 inding sites were functionally redundant for basal promoter activities in both directions.

40 105/+32 and -283/-105 bp mediate minimal and basal promoter activities, respectively.

41 C-boxes are crucial for v-Src-responsive and basal promoter activities.

42 e deletion experiment, i.e. it increased the basal promoter activity and abolished the response to et

43 oter at the NF-kappaB-binding site increased basal promoter activity and abrogated the activating and

44 -responsive element (GFRE) that confers high basal promoter activity and activation by growth factors

45 er gene activity, whereas AML1/ETO represses basal promoter activity and blocks the response to TGF-b

46 t-negative Cdc42 and Rac constructs elevated basal promoter activity and blunted the LPS response.

47 binding sites (ATTA) that are essential for basal promoter activity and cell-specific expression of

48 Because region I possesses basal promoter activity and directly mediates iNOS gene

49 P response element (CRE); both contribute to basal promoter activity and each provides an independent

50 containing either C(-)92 or G(-)92 abolishes basal promoter activity and eliminates the binding of Sp

51 tation of either Sp1 site both decreases the basal promoter activity and eliminates the induction by

52 GH, while overexpressed C/EBPdelta elevates basal promoter activity and enhances the stimulation by

53 .7 kb of the 5'-flanking region demonstrated basal promoter activity and failed to show any cytokine-

54 ted that the hMRE-a element is essential for basal promoter activity and for induction by hypoxia, bu

55 Both basal promoter activity and IL-6 responsiveness are atte

56 vator protein-1 [AP-1] site at -72 abolished basal promoter activity and LPS/zymosan inducibility, wh

57 1,25-Dihydroxyvitamin D3 does not suppress basal promoter activity and marginally suppresses parath

58 of the consensus binding site decreased the basal promoter activity and nearly eliminated transactiv

59 in the Csrp2 promoter that was critical for basal promoter activity and response to TGFbeta.

60 in the PHB promoter is required for maximal basal promoter activity and responsiveness to IL-6.

61 es in the first intron mediate repression of basal promoter activity and stimulation by activin.

62 to the TATA box region of PEG-3 in mediating basal promoter activity and the enhanced expression of P

63 F1) response element proved critical both to basal promoter activity and to PTTG and PBF repression o

64 anscription factor activity is necessary for basal promoter activity and TPA response of the involucr

65 Knockdown of PC4 by siRNA inhibited the LHR basal promoter activity and trichostatin A (TSA)-induced

66 the topo II alpha promoter reduced both the basal promoter activity and wt p53-induced suppression.

67 C/EBPbeta active form (LAP) increased hiNOS basal promoter activity approximately sixfold in liver c

68 nto C3H10T1/2 and ATDC5 cells shows a strong basal promoter activity between 565 bp and 2 kb.

69 .8 kb upstream of the NOS2 gene demonstrated basal promoter activity but failed to show any cytokine-

70 st upstream of the CCAAT box, contributes to basal promoter activity but is not involved in the cAMP

71 e-directed mutagenesis to be dispensable for basal promoter activity but suppressed the ability of th

72 ding motif within the COX-2 promoter reduced basal promoter activity by 50% whereas mutation of the N

73 ation of IGF-1 and OP-1, which decreased the basal promoter activity by 60% and almost completely abr

74 luciferase reporter vector and characterized basal promoter activity by deletion analysis.

75 oximal promoters suggests that regulation of basal promoter activity by members of the NFI transcript

76 leasing hormone [TRH] genes), stimulation of basal promoter activity by unliganded TR beta was impair

77 on assays showed that sequences required for basal promoter activity extend no further than 112 bp up

78 t pheochromocytoma) and L2 (rat lung) cells, basal promoter activity has been associated with sequenc

79 an initiator element and a region conferring basal promoter activity has been identified.

80 n gene, -180C>G, had significantly increased basal promoter activity in adipocytes.

81 onal start site was found to be required for basal promoter activity in both HepG2 and MCF-7 cells.

82 hich we demonstrate is capable of conferring basal promoter activity in Chinese Hamster Ovary cells.

83 ic domain blocked this induction and reduced basal promoter activity in every colon cancer cell line

84 AP1 element located at -270 are required for basal promoter activity in liver cells.

85 kilobase DNA fragment binds Sp1 and confers basal promoter activity in the Crp2/SmLim gene.

86 entified a minimal 252-bp region with strong basal promoter activity in transient transfection assays

87 Functional analysis indicates that basal promoter activity is conferred by the sequence fro

88 Transfection studies showed that the basal promoter activity is quite potent, being similar i

89 luciferase reporter assay demonstrated that basal promoter activity lies between bp -158 and +30 of

90 Gene reporter assay showed potent basal promoter activity of a putative CysLTR2 promoter r

91 The basal promoter activity of FR-beta resulted from synergi

92 Full basal promoter activity of hsp84 was found to be associa

93 PPRE mutations decreased the basal promoter activity of MAT2A in activated HSCs indep

94 These findings localize the basal promoter activity of RPE65, identify potential cis

95 s much as 7- to 10-fold without altering the basal promoter activity of target reporter genes.

96 Our results show increased basal promoter activity of the hAT1R gene in cells (H295

97 o the LF silencer element serves to suppress basal promoter activity of the LF gene in non-LF-express

98 gonucleotide and, like Oct-1B, activated the basal promoter activity of the mouse beta-casein gene.

99 ultures as a model system, we found that the basal promoter activity of this gene is localized to a r

100 ot to be a consequence of inhibition of ICP6 basal promoter activity or aberrant nuclear localization

101 n significantly reduced the human PDGFR-beta basal promoter activity relative to the control.

102 The ability of 243R to repress HIV-1 basal promoter activity requires both an intact N-termin

103 Mutations in both sites reduced basal promoter activity to 7% of wild type promoter acti

104 TGF-beta or integrin alpha5, this increased basal promoter activity was blocked.

105 Basal promoter activity was confined to -1,252 bp upstre

106 One of the two Sp1 sites essential for basal promoter activity was identified as critical for t

107 Basal promoter activity was low in 80% of the clones and

108 ar pattern in B cells; however, as expected, basal promoter activity was much higher in B cells as co

109 Basal promoter activity was restricted to a region spann

110 air sub-fragment that confers 70% of maximal basal promoter activity was shown to contain two synergi

111 nts 0.9-6.2 kb long exhibited unusually high basal promoter activity when transfected into the liver

112 yc binding to the p53 promoter decreased the basal promoter activity without affecting the OM-mediate

113 e element at -50 to -43 markedly reduced the basal promoter activity, and a mutation of the activator

114 The Sp1 sites mediated basal promoter activity, and both Sp1 and EGR-1 sites we

115 eat (LTR), even in the absence of detectable basal promoter activity, and this transcriptional activa

116 f the UGDH gene promoter appear to determine basal promoter activity, as does a previously unrecogniz

117 Overexpression of SRF increased basal promoter activity, but activity was still stimulat

118 fragment at the GS upstream sequence showed basal promoter activity, but failed to show any TNF-alph

119 showed that HNF3 site 1 is not required for basal promoter activity, but is essential for HNF3gamma-

120 ressive effect of 1,25(OH)2D3 by attenuating basal promoter activity, indicating that this region med

121 sponsive elements resulted in suppression of basal promoter activity, it was not necessary for transc

122 OCT binding site, results in an increase in basal promoter activity, suggesting that endogenous POU

123 appa B binding site completely abrogated the basal promoter activity, thus also rendering the promote

124 One Sp1 site, essential for basal promoter activity, was identified as critical for

125 owed that intact Sp1 sites are necessary for basal promoter activity, whereas the integrity of Egr-1

126 the TATA-like elements are both required for basal promoter activity, with enhanced activity mediated

127 n this fragment (-340 to -345) did not alter basal promoter activity.

128 tinguishable from those required for minimal basal promoter activity.

129 e (-252) contained cis-elements required for basal promoter activity.

130 and USF-1 were involved in the regulation of basal promoter activity.

131 s required for repression by p53 but not for basal promoter activity.

132 t from position -221 to +178 responsible for basal promoter activity.

133 etermined its structure and investigated its basal promoter activity.

134 both C/EBPalpha-mediated transactivation and basal promoter activity.

135 the mutated Inr led to a 2-fold increase in basal promoter activity.

136 e most important of the three in maintaining basal promoter activity.

137 around -60, each of which is responsible for basal promoter activity.

138 exon 1 were equally important in sustaining basal promoter activity.

139 oter region responsible for maintaining high basal promoter activity.

140 microM) but not zinc responsiveness, and to basal promoter activity.

141 the human PDGFR-beta promoter is crucial for basal promoter activity.

142 on and diminishes both v-Src stimulation and basal promoter activity.

143 or Skn1i, results in a strong suppression of basal promoter activity.

144 ion (approximately 7-fold) with no effect on basal promoter activity.

145 n the MIF promoter that were responsible for basal promoter activity.

146 er binding site is the critical component of basal promoter activity.

147 eas the Sp1-binding sites were important for basal promoter activity.

148 be a regulatory region for the chicken EF-2 basal promoter activity.

149 recognition sites play an important role for basal promoter activity.

150 en position -239 and -219 is essential for a basal promoter activity.

151 fied a minimal 0.3-kb region that had strong basal promoter activity.

152 ation cannot be abolished without abolishing basal promoter activity.

153 crease in response to PSI, with no change of basal promoter activity; (3) PDEF upregulates p62 promot

154 ed in the three MRF knockout alleles, with a basal promoter and a lacZ reporter.

155 uorescent protein reporter gene containing a basal promoter and a single strong P3A2 target site.

156 only a single module can associate with the basal promoter and drive gene expression at any given ti

157 In the inactive template, only the basal promoter and enhancer element remain sensitive to

158 transcription start site (tsp) distal to the basal promoter and identified two new MRP7 transcripts w

159 regulatory subfunction when linked with the basal promoter and in some cases various other modules.

160 The basal promoter and proximal enhancer fragments contain p

161 patterns: primary enhancers located near the basal promoter and secondary, or 'shadow', enhancers loc

162 utations reveal an essential Sp1 site in the basal promoter and several dispersed upstream Sp1 sites

163 the critical regulatory regions for the hRFC basal promoters and stress the functional importance of

164 independent enhancers when fused to the phas basal promoter, and did not lower expression when insert

165 it displayed activity at a distance from the basal promoter, and in both orientations.

166 igate reporter gene expression driven by the basal promoter, and no nuclear factor binds to oligonucl

167 actosidase reporter gene containing the SpHE basal promoter, and provide strong evidence that the act

168 Mutimerized P3 sites in front of a basal promoter are able to drive the expression of a rep

169 or TH AP1 site fused upstream of the TH gene basal promoter are only modestly responsive or nonrespon

170 Two of the NRF-2 sites in this basal promoter are organized in a tandem repeat.

171 target sites fused upstream of a sea urchin basal promoter are sufficient to confer accurate mesench

172 re that consensus Otx binding sites fused to basal promoters are sufficient to activate CAT reporter

173 aring nucleosomes are deposited at repressed/basal promoters but facilitate activation through their

174 romoter possessed similar composition as the basal promoter, but also contains stably bound Brg1.

175 cells, 4E increased the activity of an ADH4 basal promoter by 50-fold.

176 ack through the intervening DNA to reach the basal promoter complex and activate efficient mRNA synth

177 e a direct contact to the DNA component of a basal promoter complex to promote changes in sigma54-RNA

178 The B7-1 basal promoter consists of three positively regulated re

179 OM had no effect on the basal promoter construct pLDLR-TATA; however, including

180 The basal promoter contains a non-canonical TATA-like motif

181 The 92-bp basal promoter contains sites for the nuclear respirator

182 The basal promoter contains two functionally essential, ETS-

183 The 90-base pair basal promoter contains two SP-1 sites, one SF-1 site, a

184 This basal promoter contains two YY1 sites and at least one s

185 on to the reporter gene independently of the basal promoter context and the light-triggered morpholog

186 Basal promoters dependent on the elements for upstream a

187 ion with an NRE-like element upstream of the basal promoter directed low level green fluorescent prot

188 The basal promoter drives high constitutive expression, alth

189 separate native CATAC elements upstream of a basal promoter driving expression of either the yeast Ga

190 interactions of the TFII-D complex with the basal promoter, E-box motifs contribute to the efficient

191 m promoter or the first intron, fused to the basal promoter, each supported glutamatergic-specific ex

192 multimerized RFX binding sites in front of a basal promoter efficiently functioned in a tissue- and l

193 We show that a novel basal promoter element (-17 GCCTGCCTGGCGA -5) positioned

194 owever, in contrast, E-box1 was not a strong basal promoter element nor was it metal ions responsive

195 ated that the TATA box sequence can act as a basal promoter element not only for RNA polymerase II (R

196 EBE, for eIF4E basal element) functions as a basal promoter element that binds hnRNP K.

197 d characterization of a conserved downstream basal promoter element that is present in a subset of Dr

198 ease III protection assays, we localized the basal promoter element to a 32-bp fragment.

199 clear RNA (snRNA) promoters contain a common basal promoter element, the proximal sequence element (P

200 ulate that the DPE is a distinct, downstream basal promoter element.

201 regulated events through the mutagenesis of basal promoter elements and by altering the position and

202 analysis revealed a similar organization of basal promoter elements compared with other gammaretrovi

203 e ANK-1 promoter is CpG rich, no discernable basal promoter elements had been identified.

204 hancer-independent, and is driven instead by basal promoter elements that provide a sufficient level

205 This element, as well as two additional basal promoter elements, is divergent in sequence from t

206 ion was not dependent on the identity of the basal promoter elements, the presence of a distal enhanc

207 distal sequence elements (DSEs), and similar basal promoter elements, the proximal sequence elements

208 lect upstream regulatory elements but not by basal promoter elements.

209 but unlike Knirps, CtBP is unable to repress basal promoter elements.

210 tation of NF-kappaB-like sites 1 and 2, both basal promoter expression and response to stimulation wi

211 ' of the transcription start site acted as a basal promoter for Cx40 and that there was a strong nega

212 +959 to +1158 (within intron 1) contains the basal promoter for HYAL-2 in chondrocytes.

213 presence of a functional CRE site within the basal promoter for its suppressive activity.

214 on between -96 to +68 base pair contains the basal promoter for mVGLUT2.

215 Therefore, LAP1 is apparently the basal promoter for PrV LAT gene expression during viral

216 ons for certain DNA-binding sites within the basal promoter fragment of the gene.

217 ive only to the linear distance separating a basal promoter from an array of bound activators on DNA

218 Thus, the coactivator CIITA rescues the basal promoter from the requirement for TAF(II)250, wher

219 se studies indicate that FKHR contributes to basal promoter function and is required to mediate effec

220 onsensus motif within this domain eliminates basal promoter function.

221 members of the AP-1 family are required for basal promoter function.

222 nding elements in this region did not affect basal promoter function; however, promoter responsivenes

223 Furthermore, the SpHE basal promoter functions effectively in vegetal cells in

224 3 isoform potently transactivated the hRFC-B basal promoter; however, the short Sp3 isoforms were tra

225 strated that T antigen controls the JC virus basal promoter in a glial cell-specific manner, since T

226 uggest that T antigen activates the JC virus basal promoter in nonglial cells by interaction with the

227 CoAA positively regulates its own basal promoter in transfection assays.

228 r mutations that increase transcription from basal promoters in vivo.

229 n, TSA enhanced transcription from a minimum basal promoter, independently of the RA-responsive eleme

230 enhancer driven GUS expression from the phas basal promoter, indicating a distance dependence of the

231 However, the liver-specific apo(a) basal promoter is extremely weak and does not exhibit co

232 The Kv1.4 basal promoter is GC-rich, contains three SP1 repeats (C

233 Similarly, the SV40 basal promoter is shown to require TAF(II)250, and the p

234 otor activity rhythms shortens if a stronger basal promoter is used.

235 within first 400 nucleotides, while minimal basal promoter is within 100 nucleotides upstream of its

236 n upstream repressor selectively targets the basal promoter leading to destabilized TCF-beta-catenin

237 ombinant HNF-4 stimulated transcription from basal promoters linked to site A.

238 An SV40 basal promoter-luciferase plasmid containing a minimal B

239 -B promoter was expressed as full-length and basal promoter-luciferase reporter constructs in K562(pT

240 between the activator binding sites and the basal promoter, nor alteration of the relative helical a

241 We show that the basal promoter of a major histocompatibility complex cla

242 The 2318 bp genomic fragment contains the basal promoter of human CRLR, including potential TATA-b

243 10 microM TMPyP4 reduced the activity of the basal promoter of PDGF-A approximately 40%, relative to

244 Elements within the basal promoter of the gene do not fully explain CFTR exp

245 nts achieving this appear to lie outside the basal promoter of the gene.

246 provide evidence that cis activation of the basal promoter of the human PKCalpha gene occurs through

247 dramatically activated transcription of the basal promoter only when lac operator sequences were pre

248 486-inducible promoter activity in the KLF11 basal promoter or distal PR-binding region, both of whic

249 to be associated with specific parts of the basal promoter or further upstream regions.

250 VNTR constructs linked to the human insulin basal promoter or SV40 heterologous promoter/enhancer an

251 b region of DNA between the enhancer and the basal promoter produced a 100-fold increase in skeletal

252 The results suggest that a basal promoter produces the 1.4-kb transcript and an arg

253 ctance regulator (CFTR) gene lie outside the basal promoter region and have not been characterized.

254 Deletion analysis revealed a nonspecific basal promoter region between nucleotides -122 and -56 u

255 A basal promoter region containing within it a promoter E-

256 The basal promoter region contains potential binding sites f

257 1.1 kb between the distal GATA sites and the basal promoter region led to deregulated expression of G

258 Gel mobility shift assays with the hRFC-B basal promoter region revealed specific DNA-protein comp

259 an HNF4alpha-binding site within the CYP2C8 basal promoter region that is cis-activated by cotransfe

260 1 and NM23-H2 also cleaved within the PDGF-A basal promoter region, again exhibiting preferences for

261 rom the transcription start site) in the HGF basal promoter region, which binds to inducible transcri

262 A gain-of-function assay using basal promoter-reporter fusions in stable transgenic Ara

263 On the basal promoter, RIP140 replaces coactivators GRIP1 and P

264 /CBFalpha-related cis-acting elements in the basal promoter sequence also occurs in osteoblasts.

265 The CoAA coding and basal promoter sequences are retained within the amplico

266 the activator binding sites relative to the basal promoter should affect the degree of transcription

267 nment of the activator binding sites and the basal promoter significantly affected in vitro transcrip

268 d with two phage-encoded proteins, gp55 (the basal promoter specificity factor) and gp33 (the coactiv

269 nscription activation by CBF is dependent on basal promoter structure.

270 repression are TFIID and TFIIH, while for a basal promoter, TFIIH is the major target for mitotic in

271 the 3' half of the U3 region, including the basal promoter, the enhancer, and the putative upstream

272 When present upstream of a thymidine kinase basal promoter, the FRE exhibits high transcriptional ac

273 When inserted as a single copy upstream of a basal promoter, this composite enhancer, termed the CD28

274 ement found in the chicken betaB1-crystallin basal promoter to activate the expression of this gene.

275 tory elements (Atoh1 3' enhancer/beta-globin basal promoter) to direct expression of Clrn1 in hair ce

276 in vivo when it is artificially targeted to basal promoters via the DNA-binding domain of the yeast

277 ription; the -0.5-kb fragment containing the basal promoter was inactive in five transgenic mouse lin

278 ion of the sequence defined as X-NotI in the basal promoter were observed only in the G0/G1 phase of

279 The regulatory sequences, except the basal promoter, were mutated by either deletion or seque

280 te S-I alone induced p53 responsiveness to a basal promoter when cloned upstream from the TATA box, b

281 synergistically increase transcription of a basal promoter when targeted to DNA by fusion to a Gal4

282 tream of noncoding exon A, including a 47-bp basal promoter with a CRE/AP-1-like consensus element th

283 of the basal transcription apparatus to the basal promoter with markedly different outcomes because

284 1 promoter with peak expression in G1/S or a basal promoter with six Forkhead DNA-binding sites with

285 uncated GLYT-1b promoter constructs reveal a basal promoter within 304 base pairs of the transcriptio

286 interfere with the activity of activators or basal promoters within approximately 100 bp.